First report on determination of bioelement, vitamin content and antioxidant properties of Verbascum golawanense

Abstract

This study was designed to investigate the antioxidant, antiradical potential and element and vitamin (A, E, K and C) levels in leaf extracts of Verbascum golawanense belonging to Scrophulariaceae family. For this purpose, DPPH, ABTS, anti-haemolytic activity, total flavonoid, phenolic content, total antioxidant capacity and vitamin C content were determined and measured spectrophotometrically. Vitamin A, E and K were analysed by HPLC and elemental analysis was performed by ICP-OES method. Phenolic, flavonoid and antioxidant capacity of the plant were determined as 5.861 ± 0.212 mg GA/g, 26.422 ± 0.613 mg QE/g and 124.359 ± 2.562 mM AA/g, respectively. K, Na, Fe, V, retinol, α-tocopherol, phylloquinone and vitamin C were determined as 3.086 ± 0.0131 mmol/kg, 2.260 ± 0.0266 mmol/kg, 1.1465 ± 0.0731 mmol/kg, 29.326 ± 3.072 µmol/kg, 0.1107 ± 0.013 µmol/kg, 0.4002 ± 0.16 µmol/kg, 1.101 ± 0.118 µmol/kg and 2552.0126 ± 187.056 mg/100g, respectively. The IC50 values related to the in vitro antioxidant properties of DPPH, ABTS and anti-haemolytic activity assays were 19.170 ± 0.615 μg/mL, 26.877 ± 0.461 μg/mL and 64.87 ± 2.24 μg/mL. In vitro studies revealed that the plant has a good antioxidant potential. It was determined that the vitamin and element content was high and total phenol, flavonoid and antioxidant potential were at reasonable levels. ABTS and DPPH tests showed a promising antioxidant power. These results indicated that the extract could be a potential alternative and could be investigated for the discovery and development of new chemical compounds for the treatment of various diseases.

Keywords

• Verbascum golawanense
• Antioxidant activity
• Methanol extract
• Bioelement
• Vitamin

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