Antimicrobial activity of ozonated sunflower oil against Pseudomonas aeruginosa strains isolated from mares, and interactions with antimicrobial drugs

Arthur Azevedo Perpétuo; Gabriel Souza Dos Santos; Marcus Vinícius Dias Souza

doi:10.21448/ijsm.1606326

Antimicrobial activity of ozonated sunflower oil against Pseudomonas aeruginosa strains isolated from mares, and interactions with antimicrobial drugs

Abstract

Infectious endometritis in mares can result in reduced fertility, early embryonic loss, and general health decline, implicating extensive economic loss. Pseudomonas aeruginosa is an opportunistic pathogen that is among the most frequent bacterial pathogens associated with infectious endometritis in mares. Here we explored the antimicrobial potential of ozonated sunflower seed oil, compared to the conventional form (both widely explored for wound healing), against clinical isolates of Pseudomonas aeruginosa obtained from mares with endometritis. The effects of combining the oils with clinically relevant antimicrobial drugs were also investigated. The conventional form of the oil was active against the strains, whereas the ozonated oil was not. When combined with the antimicrobials, both oils significantly decreased the pharmacological activity of the drugs. This study opens doors for discussions on clinical protocols for treating fertility issues in mares, as well as bacterial resistance in the intrauterine environment.

Keywords

Supporting Institution

No institution supported financially this study.

Ethical Statement

We have no ethical issues to report in this paper.

Thanks

No thanks to inform.

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Details

Primary Language

English

Subjects

Pharmaceutical Microbiology

Journal Section

Research Article

Authors

Arthur Azevedo Perpétuo This is me
0000-0002-3863-8718
Brazil

Gabriel Souza Dos Santos This is me
0000-0002-2801-0214
Brazil

Marcus Vinícius Dias Souza ^*
0000-0001-5723-5095
Brazil

Early Pub Date

September 1, 2025

Publication Date

December 5, 2025

Submission Date

December 28, 2024

Acceptance Date

May 9, 2025

Published in Issue

Year 2025 Volume: 12 Number: 4

DOI

https://doi.org/10.21448/ijsm.1606326

IZ

https://izlik.org/JA64LB64PW

Cite

RIS / Bibtex

APA

Azevedo Perpétuo, A., Souza Dos Santos, G., & Dias Souza, M. V. (2025). Antimicrobial activity of ozonated sunflower oil against Pseudomonas aeruginosa strains isolated from mares, and interactions with antimicrobial drugs. International Journal of Secondary Metabolite, 12(4), 808-819. https://doi.org/10.21448/ijsm.1606326

AMA

1.Azevedo Perpétuo A, Souza Dos Santos G, Dias Souza MV. Antimicrobial activity of ozonated sunflower oil against Pseudomonas aeruginosa strains isolated from mares, and interactions with antimicrobial drugs. Int. J. Sec. Metabolite. 2025;12(4):808-819. doi:10.21448/ijsm.1606326

Chicago

Azevedo Perpétuo, Arthur, Gabriel Souza Dos Santos, and Marcus Vinícius Dias Souza. 2025. “Antimicrobial Activity of Ozonated Sunflower Oil Against Pseudomonas Aeruginosa Strains Isolated from Mares, and Interactions With Antimicrobial Drugs”. International Journal of Secondary Metabolite 12 (4): 808-19. https://doi.org/10.21448/ijsm.1606326.

EndNote

Azevedo Perpétuo A, Souza Dos Santos G, Dias Souza MV (December 1, 2025) Antimicrobial activity of ozonated sunflower oil against Pseudomonas aeruginosa strains isolated from mares, and interactions with antimicrobial drugs. International Journal of Secondary Metabolite 12 4 808–819.

IEEE

[1]A. Azevedo Perpétuo, G. Souza Dos Santos, and M. V. Dias Souza, “Antimicrobial activity of ozonated sunflower oil against Pseudomonas aeruginosa strains isolated from mares, and interactions with antimicrobial drugs”, Int. J. Sec. Metabolite, vol. 12, no. 4, pp. 808–819, Dec. 2025, doi: 10.21448/ijsm.1606326.

ISNAD

Azevedo Perpétuo, Arthur - Souza Dos Santos, Gabriel - Dias Souza, Marcus Vinícius. “Antimicrobial Activity of Ozonated Sunflower Oil Against Pseudomonas Aeruginosa Strains Isolated from Mares, and Interactions With Antimicrobial Drugs”. International Journal of Secondary Metabolite 12/4 (December 1, 2025): 808-819. https://doi.org/10.21448/ijsm.1606326.

JAMA

1.Azevedo Perpétuo A, Souza Dos Santos G, Dias Souza MV. Antimicrobial activity of ozonated sunflower oil against Pseudomonas aeruginosa strains isolated from mares, and interactions with antimicrobial drugs. Int. J. Sec. Metabolite. 2025;12:808–819.

MLA

Azevedo Perpétuo, Arthur, et al. “Antimicrobial Activity of Ozonated Sunflower Oil Against Pseudomonas Aeruginosa Strains Isolated from Mares, and Interactions With Antimicrobial Drugs”. International Journal of Secondary Metabolite, vol. 12, no. 4, Dec. 2025, pp. 808-19, doi:10.21448/ijsm.1606326.

Vancouver

1.Arthur Azevedo Perpétuo, Gabriel Souza Dos Santos, Marcus Vinícius Dias Souza. Antimicrobial activity of ozonated sunflower oil against Pseudomonas aeruginosa strains isolated from mares, and interactions with antimicrobial drugs. Int. J. Sec. Metabolite. 2025 Dec. 1;12(4):808-19. doi:10.21448/ijsm.1606326

Cited By

Exploring Ozonated Vegetable Oils as Antimicrobial and Functional Agents in Food Systems: A Systematic Narrative Review

Foods

https://doi.org/10.3390/foods15111850