Dosage of phenolic compounds and evaluation of anti-inflammatory activity in vivo of Rubia tinctorum extract

Year 2025, Volume: 12 Issue: 4, 861 - 867, 05.12.2025

Amine Bengag , Rouam Djawed , Meziane Ahmed Malika

https://doi.org/10.21448/ijsm.1611679

Abstract

This study is part of the search for new biologically active molecules of plant origin. The aim of this study is the in vivo evaluation of the anti-inflammatory activity of the hydro-methanolic extract of the underground part (roots) of Rubia tinctorum from the Wilaya of Chlef (Algeria). The quantitative estimation of polyphenols, flavonoids, and tannins by the colorimetric method showed that the extract studied is rich in these compounds, with contents equal to 199.17 ± 0.074 mg GAE/g, 53.65 ± 0.042 mg QE/g, and 45.74 ± 0.033 mg CE/g, respectively. The anti-inflammatory activity was evaluated using the model of paw edema induced by the intraperitoneal (IP) route in mice (Morini strain) by carrageenan. The results obtained were compared to those of the reference treatment (diclofenac sodium). The evaluation of the percentage of inhibition of the hydro-methanolic extract of the root of Rubia tinctorum at doses of 25, 50, and 100 mg/kg significantly prevented paw edema (p<0.05) after 2 hours (25.07 ± 0.054%, 30.05 ± 0.06%, and 36.31 ± 0.014%, respectively) and after 3 hours (30.50 ± 0.08%, 34.25 ± 0.003%, and 37.8 ± 0.012%, respectively). The anti-edematous effect of the dry extract is due to its richness in tannins and phenolic compounds, which would have an inhibitory action on the mediators of inflammation. These compounds would therefore have interesting anti-inflammatory properties, suggesting a therapeutic application to prevent the inflammatory process.

Keywords

Anti-inflammatory , Rubia tinctorum , Polyphenols

Ethical Statement

ANDRS-NRP-EC- No: 1049/2024. National Agency for the Development of Health Research (ANDRS), National Research Project (NRP).

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