A Comparative Study on Antioxidant and Antibacterial Activities of Four Senecio Species From Turkey

Abstract

The present study was conducted to investigate total phenolic content, antioxidant and antibacterial activity of four Senecio L. (Asteraceae). The in vitro antioxidant activities of extracts were evaluated with different antioxidant testing systems. Their antioxidant activities were assessed by phosphomolybdenum reduction, DPPH and β-carotene bleaching assay. The extracts exhibited strong antioxidant activity and high scavenging activities against DPPH free radicals. While the extracts exhibited weak to moderate β-carotene bleaching activity. The antimicrobial activity of Senecio extracts was tested against 15 microorganisms using agar diffusion and broth microdilution assays. The extracts were found to be weak to moderate effective against 8 out of the 15 microorganisms tested with minimal inhibitory concentration (MIC) values ranging from 1.5 to 12.5 mg/ml. In conclusion, it can be concluded that Senecio extracts may be considered as natural sources in many industry such as food and pharmacy.

Keywords

• Senecio
• phenolic
• antioxidant activity
• antimicrobial activity

References

1. Ak, T., & Gulcin, İ. (2008). Antioxidant and radical scavenging properties of curcumin. Chemico Biological Interaction, 174, 27-37.
2. Albayrak, S., Aksoy, A., Hamzaoğlu, E., Ekici, L., & Budak, U. (2008). Antimicrobial and antioxidant activities of Senecio species growing in the black Sea region. Turkey. Acta Botonica Gallica, 155 (3), 447-456.
3. Albayrak, S., Aksoy, A., Yurtseven, L., & Yaşar, A. (2014). A comparative study on phenolic components and biological activity of some Senecio species in Turkey. Journal of Pharmacy and Pharmacology, 66, 1631-1640.
4. Cao, L., Si, J. Y., Liu, Y., Sun, H., Jin, W., Li, Z., Zhao, X. H., & Pan, R. L. (2009). Essential oil composition, antimicrobial and antioxidant properties of Mosla chinensis Maxim. Food Chemistry, 115, 801-805.
5. Christov, V., Mikhova, B., Alexandrova, R., Dimitrova, D., Nikolova, E., & Evstatieva, L. (2002). Alkaloids from the Roots of Senecio macedonicus Griseb. Verlag der Zeitschrift für Naturforschung, 57c, 780-784.
6. Conforti, F., Loizzo, M. R., Statti, G. A., Houghton, P.J., & Menichini, F. (2006b). Biological properties of different extracts of two Senecio species. International Journal of Food Sciences and Nutrition, 57(1/2),1-8.
7. Conforti, F., Marrelli, M., Statti, G., & Meninchini, F. (2006 a). Antioxidant and cytotoxic activities of methanolic extract and fractions from Senecio gibbosus subsp. gibbosus (GUSS) DC. Natural Product Research, 20(9), 805-812.
8. El-Shazly, A., Dorai, G., & Wink, M. (2002). Chemical composition and biological activity of the essenial oils of Senecio aegyptius var. discoideus. Boiss. Verlag der Zeitschrift für Naturforschung, 57c, 434-439.

9. Kiprono, P. C., Kaberia, F., Keriko, J. M., & Karanja, J. N. (2000). The in vitro antifungal and anti-bacterial activities of β–sitosterol from Senecio lyratus (Asteraceae). Verlag der Zeitschrift für Naturforschung, 55c, 485-488.
10. Kulisic, T., Radonic, A., Katalinic, V., & Milos, M. (2004). Use of different methods for testing antioxidative activity of oregano essential oil. Food Chemistry, 85, 633-640.
11. Lee, S. K., Mbwambo, Z. H., Chung, H. S., Luyengi, L., Games, E. J., Mehta, R. G., Kinghorn, A. D., & Pezzuto, J. M. (1998). Evaluation of the antioxidant potential of natural products. Combinatorial Chemistry & High Throughput Screening, 1, 35-46.
12. Liu, F., & Ng, T. B. (2000). Antioxidative and Free Radical Scavenging Activities of Selected Medicinal Herbs. Life Sciences, 66(8), 725-735.
13. Loizzo, M.R., Statti, G.A., Tundis, R., Conforti, F., Bonesi, M., Autelitano, G., Houghton, P.J., Miljkovic-Brake, A., & Menichini, F. (2004). Antibacterial and antifungal activity of Senecio inaediquans DC and Senecio vulgaris L. Phytotherapy Research, 18, 777- 779.
14. Loizzo, M. R., Tundis, R., Statti, G. A., Miljkovic-Brake, A., Menichini, F., & Houghton, P. J. (2006). Bioactive extracts from Senecio samnitum Huet. Natural Product Research, 20(3), 265-269.
15. Lu Y., &Foo L. Y. (2001). Antioxidant activities of polyphenols from sage (Salvia officinalis). Food Chemistry, 75, 197-202.
16. Maisarah, A. M., Nurul Amira, B., Asmah, R., & Fauziah, O. (2013). Antioxidant analysis of different parts of Carica papaya. International Food Research Journal, 20(3), 1043- 1048.
17. Pérez-Castorena, A. L., Arciniegas, A., Villaseñor, J. L., & de Vivar A. R. (1999). Alkaloids from three Senecio species. Biochemical Systematics and Ecology, 27, 835-837.
18. Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of phosphomolybdenum complex: specific application to determination of vitamin E. Analytical Biochemistry, 269, 337-341.
19. Sagdic, O., Aksoy, A., & Ozkan G. (2006). Evaluation of the antibacterial and antioxidant potentials of gilaburu (Viburnum opulus L.) fruit extract. Acta Alimentaria, 35(4), 487- 492.
20. Singleton, V. L., & Rossi, J. A. (1995). Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-15.
21. Sokmen, A., Gulluce, M., Akpulat, H. A., Daferera, D., Tepe, B., Polissiou, M., Sokmen, M., & Sahin, F. (2004). The in vitro antimicrobial and antioxidant activities of the essential oils and methanol extracts of endemic Thymus spathulifolius. Food Control, 15, 627- 634.
22. SPSS; SPSS Version 10.0., (2001). SPSS Inc, 233 S. Wacker Drive, Chcago, Illinois.
23. Tundis, R., Loizzo, M. R., Statti, G. A., Houghton, P. J., Miljkovic-Brake, A., & Menichini, F. (2006). In vitro hypoglycemic and antimicrobial activities of Senecio leucanthemifolius Poiret. Natural Product Research, 1-5.
24. Uğur, A., Ertem, H., & Beyatlı, Y. (2006). Antibacterial Properties of Senecio sandrasicus on Multidrug-Resistant Stenotrophomonas maltophilia. Pharmaceutical Biology, 44(4), 253-257.
25. Unal E. L., Mavi A., Kara A. A, Cakir A., & Şengül, M., Yildirim, A. (2008). Antimicrobial and Antioxidant Activities of Some Plants Used as Remedies in Turkish Traditional Medicine. Pharmaceutical Biology, 46 (3), 207-224.
26. Uzun, E., Sariyar, G., Karakoc, B., Otuk, G., Oktayoglu, E., & Pirildar, S. (2004). Traditional medicine in Sakarya province (Turkey) and antimicrobial activities of selected species. Journal of Ethnopharmacology, 95, 287-296.
27. Wang S. Y., & Ballington, J. R. (2007). Free radical scavenging capacity and antioxidant enzyme activity in deerberry (Vaccinium stamineum L.). LWT- Food Science and Technology, 40, 1352-1361.
28. Wu C., Chen F., Wang X., Kim H. J., He G., Haley-Zitlin V., & Huang G. (2006a). Antioxidant constituents in feverfew (Tanacetum parthenium) extract and their chromatographic quantification. Food Chemistry, 96, 220-227.
29. Wu, B., Lin, W. H., Gao, H. Y., Zheng, L., Wu, L. J., & Kim, C. S. (2006b). Four New Antibacterial Constituents from Senecio cannabifolius. Pharmaceutical Biology, 44(6), 440-444.
30. Zampini I. C., Vattuone M. A., Isla M. I. (2005). Antibacterial activity of Zuccagnia punctata Cav. ethanolic extracts. Journal of Ethnopharmacology, 102, 450-456.
31. Zhao G. R., Xiang Z. J., Ye T. X., Yuan Y. J., Guo Z. X. (2006). Antioxidant activities of Salvia miltiorrhiza and Panax notoginseng. Food Chemistry, 99, 767-774.