2,4-Dimetilpirol’ün Başak Yanıklığı Hastalığı Etmeni Fusarium culmorum’un Üzerine Etkilerinin İncelenmesi

Öz

Bitki patojeni Fusarium culmorum dünyada ve ülkemizde tahıllar üzerinde kök çürüklüğü ve başak yanıklığı başta olmak üzere çeşitli hastalıklara neden olmaktadır. Bu çalışmada F. culmorum ile mücadele kullanılabilecek yeni ve potansiyel bir ajan olarak 2,4-Dimetilpirol’ün patojen üzerindeki etkileri incelenmiştir. Bu amaçla farklı konsantrasyonlarda 2,4-Dimetilpirol (0, 0.5, 1, 2 ve 4 mg mL^-1) uygulanmış F. culmorum 20F izolatında artan konsantrasyonlarda doğrusal büyüme oranındaki (DBO)  değişim incelenmiş ve kontrol ve deney grupları arasında anlamlı bir düşüş olduğu görülmüştür.  Ayrıca seksüel üreme ve hücre çeperi bütünlüğünden sorumlu Mgv1 geninin anlatımı gerçek zamanlı kantitatif polimeraz zincir reaksiyonu (k-PZR) ve ters transkripsiyon PZR (RT- PZR) ile incelenmiştir. Deney gruplarında kontrol gruplarına göre Mgv1 geni için 5.21±0.05x10² kat artma saptanmıştır. Elde edilen bulgulara göre 2,4-Dimetilpirol’ün, F. culmorum türünde potansiyel bir antifungal etkili ajan olabileceği gösterilmiştir. İleriki çalışmalarda bu ajanın tarladaki fungal biyokütlenin ve toksin üretiminin azaltılmasını sağlayarak hastalık ile mücadelede yeni bir yaklaşım olarak kullanılabileceği düşünülmektedir.

Anahtar Kelimeler

• Antifungal,Başak Yanıklığı,Fusarium,2.4-Dimetilpirol

Investigation of the Effects of 2,4-Dimethylpyrrole on Fusarium culmorum Causal Agent of Head Blight Disease

Abstract

Fusarium culmorum, a phytopathogen, cause several diseases including root rot and head blight on cereals in worldwide and our country. In this study, effects of 2,4-Dimethylpyrrole, as a novel and potential agent on pathogen which can be used in fight with F. culmorum were investigated. For this purpose, alteration in linear growth rate (LGR) in F. culmorum 20F isolate, subjected to different concentrations of 2,4-Dimethylpyrrole (0, 0.5, 1, 2, 4 mg mL^-1) was investigated and the significant decrase between control and experiment sets was determined. Besides, expression of Mgv1 gene, responsible for sexual stage and cell wall integrity, was investigated via quantitative real time polymerase chain reaction (q-PCR) and reverse transcription PCR (RT-PCR). In comparison to control groups, 5.21±0.05x10² fold increases in experiment set for Mgv1 gene was detected. According to findings obtained, it was shown that 2,4-Dimethylpyrrole could be a potential antifungal agent for F. culmorum. In further works, it is suggested that this agent could be used as a novel approach in disease control including inhibition of fungal biomass and toxin production in field.

Keywords

• Antifungal,Head Blight,Fusarium,2.4-Dimethylpyrrole

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