Effects of Osmotic Conditioning Treatments of Lavender (Lavandula angustifolia) Seeds on Mean Germination Time and Germination Rate

Öz

Lavender is an important perfume, cosmetic and pharmaceutical plant worldwide because of its high quality and quantity essential oil content. Besides, it is an important ornamental plant with pleasant fragrance and beautiful vegetative pattern in bush form. In seed propagation, there are some problems such as low germination times and germination rate. The aim of this study was to investigate the effects of some selected osmotic conditioners on lavender seeds. Osmotic conditioning (OC) was performed in seeds with methyl jasmonate (MeJA) (1.0 mM) and seaweed (Ascophyllum nodosum) extract (1:500 seaweed extract) at 20°C for 1 and 2 days. After the treatment, seeds were characterized for germination tests at 25°C. Germination indexes of the seeds, germination rates and mean germination time were determined. Seaweed treatment for 1 and 2 day applications and MeJA treatment for 2 days significantly increased the germination rates in lavender seeds compared to the control. In addition seaweed 2 day treatment and MeJA 2 day treatment also increased the germination index. In conclusion, this study indicated that both MeJA and seaweed treatments had positive effects on germination parameters studied in lavender seeds.

Anahtar Kelimeler

• Lavender
• osmotic conditioning
• MeJA
• seaweed
• germination time
• germination rate

Effects of Osmotic Conditioning Treatments of Lavender (Lavandula angustifolia) Seeds on Mean Germination Time and Germination Rate

Abstract

Lavender is an important perfume, cosmetic and pharmaceutical plant worldwide because of its high quality and quantity essential oil content.  Besides, it is an important ornamental plant with pleasant fragrance and beautiful vegetative pattern in bush form. In seed propagation, there are some problems such as low germination times and germination rate. The aim of this study was to investigate the effects of some selected osmotic conditioners on lavender seeds. Osmotic conditioning (OC) was performed in seeds with methyl jasmonate (MeJA) (1.0 mM) and seaweed (Ascophyllum nodosum) extract (1:500 seaweed extract) at 20°C for 1 and 2 days. After the treatment, seeds were characterized for germination tests at 25°C. Germination indexes of the seeds, germination rates and mean germination time were determined. Seaweed treatment for 1 and 2 day applications and MeJA treatment for 2 days significantly increased the germination rates in lavender seeds compared to the control. In addition seaweed 2 day treatment and MeJA 2 day treatment also increased the germination index. In conclusion, this study indicated that both MeJA and seaweed treatments had positive effects on germination parameters studied in lavender seeds.

Keywords

• Lavender
• osmotic conditioning
• MeJA
• seaweed
• germination time
• germination rate

References

1. Guenther, E. (1952). The essential oils. Vol. 5.
2. Kara, N. (2011). Determination of lavender (Lavandula sp.) cultivars suitable for essential oil production and studies on micropropagation possibilities. Suleyman Demirel University, Graduate School of Applied and Natural Sciences, Department of Field Crops. PhD, Süleyman Demirel University, Isparta, Turkey (In Turkish with an abstract in English).
3. Duda, M. M., Imbrea, F., Vârban, D., & Muntean, S. (2010). Research on values of 1000 seeds weight in some medicinal plants. Research Journal of Agricultural Science, 42(1), 101-104.
4. Andrade, L. B., Echeverrigaray, S., Fracaro, F., Pauletti, G. F., & Rota, L. (1999). The effect of growth regulators on shoot propagation and rooting of common lavender (Lavandula vera DC). Plant cell, tissue and organ culture, 56(2), 79-83.
5. Arin, L., & Kiyak, Y. (2003). The effects of pre-sowing treatments on emergence and seedling growth of tomato seed (Lycopersicon esculentum Mill.) under several stress conditions. Pakistan Journal of Biological Sciences, 6(11), 990-994.
6. Sivritepe, H. Ö. (2000). Deniz Yosunu Ekstraktı (Ascophyllum nodosum) ile Yapılan Ozmotik Koşullandırma Uygulamalarının Biber Tohumlarında Canlılık Üzerine Etkileri. III. Sebze Tarımı Sempozyumu, 11-13.
7. Demirkaya, M. (2016). Deniz yosunu (Ascophyllum nodosum) ekstraktı uygulamalarının biber ve soğan tohumlarının canlılığı ve gücüne etkileri/Effects of Seaweed (Ascophyllum nodosum) extract application on viability and vigour of pepper and onion seeds. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 26(3).
8. Demirkaya, M. (2012). Deniz Yosunu (Ascophyllum nodosum) Ekstraktı Uygulamalarının Domates Tohumlarının Canlılığı ve Gücüne Etkileri. alatarım, 11(1), 2012.

9. Korkmaz, A., Tiryaki, I., Nas, M. N., & Ozbay, N. (2004). Inclusion of plant growth regulators into priming solution improves low-temperature germination and emergence of watermelon seeds. Canadian journal of plant science, 84(4), 1161-1165.
10. Demirkaya, M. (2016). Metil Jasmonat ve Deniz Yosunu (Ascophyllum nodosum) Ekstraktı ile Ozmotik Koşullandırma Uygulamalarının Düşük Sıcaklıta Biber Tohumlarının Çimlenme ve Ortalama Çimlenme Süresi Üzerine Etkileri. Selçuk Tarım Bilimleri Dergisi, 3(1), 42-47.
11. Coşkun, G., Gülşen, O., & Demirkaya, M. (2016). Çerezlik kabak tohumlarında bazı ön uygulamaların çimlenme üzerine etkileri/Effects of some pretreatments on germination of pumpkin seed. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 32(1).
12. Sivritepe, H. Ö., & Eris, A. (1998, August). The effects of post-storage priming treatments on viability and repair of genetic damage in pea seeds. In XXV International Horticultural Congress, Part 7: Quality of Horticultural Products, 517 (pp. 143-150).
13. Özkan Sivritepe, H., & Demirkaya, M. (2011, October). Does humidification technique accomplish physiological enhancement better than priming in onion seeds?. In V Balkan Symposium on Vegetables and Potatoes 960 (pp. 237-244).
14. Cayuela, E., Pérez‐Alfocea, F., Caro, M., & Bolarin, M. C. (1996). Priming of seeds with NaCl induces physiological changes in tomato plants grown under salt stress. Physiologia Plantarum, 96(2), 231-236.
15. Demirkaya, M. (2014). Improvement in tolerance to salt stress during tomato cultivation. Turkish Journal of Biology, 38(2), 193-199.
16. ISTA (2005) International Seed Testing Association, p. 10. Bassersdorf, Switzerland,
17. Bekendam, J., & Grob, R. (1979). Handbook for seedling evaluation. Handbook for seedling evaluation., (Ed. 2).
18. Ellis, R. H., & Roberts, E. H. (1981). The quantification of ageing and survival in orthodox seeds. Seed Science and Technology (Netherlands).
19. Copeland, L. O., & McDonald, M. F. (2012). Principles of seed science and technology. Springer Science & Business Media.
20. Robu, S., Aprotosoaie, A. C., Spac, A., Cioancă, O., Hăncianu, M., & Stănescu, U. (2011). Studies regarding chemical composition of lavender volatile oils. Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi, 115(2), 584-589.