Antibacterial Effects of Methanol Extracts of Some Plant Species Belonging to Lamiaceae Family

Abstract

Species belonging to Lamiaceae family are used as an antibacterial agent among medical plants. The aim of the present study was to determinate the antibacterial effects of the methanolic extracts of some plant species belonging Lamiaceae family which distributed to different ecological conditions in Amasya. The antibacterial effects of Stachys annua, Scutellaria salviifolia and Nepata nuda plant extracts were determined by disc diffusion and micro dilution methods. The antibacterial effects of plant extracts were tested against standard strains of Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218, Klebsiella pneumoniae ATCC 70600, Pseudomonas aeruginosa ATCC 27853 and Salmonella enteritidis ATCC 13076. We observed that plant extracts had antibacterial effect when they compared with control group antibiotics. According to the results of disc diffusion method, the highest antibacterial effect was identified in Scutellaria salviifolia, Stachys annua and Nepata nuda, respectively. The microdilution method was studied concentration range from 6.25 - to 50 mg/ml. MICs of the most effective Scutellaria salviifolia plant extract were 12.5 mg/ml for S. aureus, S. enteritidis and E. coli; 25 mg/ml for K. pneumoniae and P. aeruginosa. The MICs of Stachys annua extract were 12.5 mg/ml for P. aeruginosa, S. enteritidis and E. coli; 25 mg/ml for S. aureus and 50 mg/ml for K. pneumoniae. MICs of Nepata nuda were 12.5 mg/ml K. pneumonia; 25 mg/ml for S. aureus; 50 mg/ml for P. aeruginosa, S. enteritidis and E. coli. This study showed that Scutellaria salviifolia, Stachys annua and Nepata nuda species have in vitro antibacterial activities.

Keywords

• Lamiaceae
• Disc diffusion
• Micro dilution

Antibacterial Effects of Methanol Extracts of Some Plant Species Belonging to Lamiaceae Family

Abstract

Species belonging to Lamiaceae family are used as an antibacterial agent among medical plants. The aim of the present study was to determinate the antibacterial effects of the methanolic extracts of some plant species belonging Lamiaceae family which distributed to different ecological conditions in Amasya. The antibacterial effects of Stachys annua, Scutellaria salviifolia and Nepata nuda plant extracts were determined by disc diffusion and micro dilution methods. The antibacterial effects of plant extracts were tested against standard strains of Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218, Klebsiella pneumoniae ATCC 70600, Pseudomonas aeruginosa ATCC 27853 and Salmonella enteritidis ATCC 13076. We observed that plant extracts had antibacterial effect when they compared with control group antibiotics. According to the results of disc diffusion method, the highest antibacterial effect was identified in Scutellaria salviifolia, Stachys annua and Nepata nuda, respectively. The microdilution method was studied concentration range from 6.25 - to 50 mg/ml. MICs of the most effective Scutellaria salviifolia plant extract were 12.5 mg/ml for S. aureus, S. enteritidis and E. coli; 25 mg/ml for K. pneumoniae and P. aeruginosa. The MICs of Stachys annua extract were 12.5 mg/ml for P. aeruginosa, S. enteritidis and E. coli; 25 mg/ml for S. aureus and 50 mg/ml for K. pneumoniae. MICs of Nepata nuda were 12.5 mg/ml K. pneumonia; 25 mg/ml for S. aureus; 50 mg/ml for P. aeruginosa, S. enteritidis and E. coli.  This study showed that Scutellaria salviifolia, Stachys annua and Nepata nuda species have in vitro antibacterial activities.

Keywords

• Lamiaceae
• Disc diffusion
• Micro dilution

References

1. Kumarasamy, Y., Cox, P. J., Jaspars, M., Nahar, L., & Sarker, S. D. (2002). Screening seeds of Scottish plants for antibacterial activity. Journal of Ethnopharmacology, 83(1), 73-77.
2. Kianbakht, S., & Jahaniani, F. (2003). Evaluation of antibacterial activity of Tribulus terrestris L. growing in Iran. Iranian Journal of Pharmacology & Therapeutics (IJPT), 2(1), 22-24.
3. Chung, P. Y., Chung, L. Y., Ngeow, Y. F., Goh, S. H., & Imiyabir, Z. (2004). Antimicrobial activities of Malaysian plant species. Pharmaceutical biology, 42(4-5), 292-300.
4. Unal, E. L., Mavi, A., Kara, A. A., Cakir, A., Şengül, M., & Yildirim, A. (2008). Antimicrobial and antioxidant activities of some plants used as remedies in Turkish traditional medicine. Pharmaceutical Biology, 46(3), 207-224.
5. Kunduhoglu, B., Pilatin, S., & Caliskan, F. (2011). Antimicrobial screening of some medicinal plants collected from Eskisehir, Turkey. Fresenius Environmental Bulletin, 20(4), 945-952.
6. Essawi, T., & Srour, M. (2000). Screening of some Palestinian medicinal plants for antibacterial activity. Journal of Ethnopharmacology, 70(3), 343-349.
7. Saxena, V. K., & Sharma, R. N. (1999). Antimicrobial activity of the essential oil of Toddalia asiatica. Fitoterapia, 70(1), 64-66.
8. Nimri, L. F., Meqdam, M. M., & Alkofahi, A. (1999). Antibacterial activity of Jordanian medicinal plants. Pharmaceutical biology, 37(3), 196-201.

9. Sarac, N., & Ugur, A. (2007). Antimicrobial activities and usage in folkloric medicine of some Lamiaceae species growing in Mugla, Turkey. EurAsian Journal of BioSciences, 4, 28-37.
10. Hammer, K. A., Carson, C. F., & Riley, T. V. (1999). Antimicrobial activity of essential oils and other plant extracts. Journal of applied microbiology, 86(6), 985-990.
11. Tipu, M. A., Akhtar, M. S., Anjum, M. I., & Raja, M. L. (2006). New dimension of medicinal plants as animal feed. Pak Vet J, 26(26), 144-148.
12. Mitscher, L. A., Drake, S., Gollapudi, S. R., & Okwute, S. K. (1987). A modern look at folkloric use of anti-infective agents. Journal of natural products, 50(6), 1025-1040.
13. Eloff, J. N. (1998). Which extractant should be used for the screening and isolation of antimicrobial components from plants?. Journal of ethnopharmacology, 60(1), 1-8.
14. Bhaskarwar, B. H. U. S. H. A. N., Itankar, P., & Fulke, A. (2008). Evaluation of antimicrobial activity of medicinal plant Jatropha podagrica (Hook). Roumanian Biotechnological Letters, 13(5), 3873-3877.
15. Raja, R. R. (2012). Medicinally potential plants of Labiatae (Lamiaceae) family: an overview. Research journal of medicinal plant, 6(3), 203-213.
16. Baser, K. H. C. (1992, July). Essential oils of Anatolian Labiatae: a profile. In WOCMAP I-Medicinal and Aromatic Plants Conference: part 1 of 4, 333, 217-238.
17. Nemeth, E., & Bernath, J. (2008). Biological activities of yarrow species (Achillea spp.). Current pharmaceutical design, 14(29), 3151-3167.
18. Si, X. T., Zhang, M. L., Shi, Q. W., & Kiyota, H. (2006). Chemical constituents of the plants in the genus Achillea. Chemistry & biodiversity, 3(11), 1163-1180.
19. Baytop, T. (1999). Therapy with medicinal plants in Turkey (Past and Present).
20. Salem, M. Z., Ali, H. M., El-Shanhorey, N. A., & Abdel-Megeed, A. (2013). Evaluation of extracts and essential oil from Callistemon viminalis leaves: Antibacterial and antioxidant activities, total phenolic and flavonoid contents. Asian Pacific journal of tropical medicine, 6(10), 785-791.
21. Wayne, P. A. (2010). Clinical and Laboratory Standards Institute (CLSI); 2010. Performance standards for antimicrobial susceptibility testing, 20.