Molecular identification and phytochemical profiling of kamiling (wild toxic plant) using thin layer chromatography

Abstract

Some plants may only be known by the people in their own respective place. Consequently, there is a need for the authentication and further study of wild toxic plant known by indigenous people. The genomic DNA from the leaves of the plant Kamiling collected in Imugan, Santa Fe, Nueva Vizcaya, Philippines was extracted and amplified using matK and rbcL gene markers. Sample sequences was queried on GenBank using Basic Local Alignment Search Tool (BLAST) revealed that the sample sequences were identified as Semecarpus cuneiformis using both on matK and rbcL markers. The leaves of Kamiling was subjected through Thin Layer Chromatography (TLC) and it revealed important phytochemicals such as alkaloids, coumarins, anthraquinones, anthrones, tannins, flavonoids, higher alcohols, phenols, steroids and essential oils. Since there is a growing awareness in correlating the phytochemical constituents of plants with their pharmacological activity, wild toxic plant can be one of the preferences for medicinal uses.

Keywords

• TLC
• matK
• rbcL
• wild toxic plant

Molecular identification and phytochemical profiling of kamiling (wild toxic plant) using thin layer chromatography

Abstract

Some plants may only be known by the people in their own respective place. Consequently, there is a need for the authentication and further study of wild toxic plant known by indigenous people. The genomic DNA from the leaves of the plant Kamiling collected in Imugan, Santa Fe, Nueva Vizcaya, Philippines was extracted and amplified using matK and rbcL gene markers. Sample sequences was queried on GenBank using Basic Local Alignment Search Tool (BLAST) revealed that the sample sequences were identified as Semecarpus cuneiformis using both on matK and rbcL markers. The leaves of Kamiling was subjected through Thin Layer Chromatography (TLC) and it revealed important phytochemicals such as alkaloids, coumarins, anthraquinones, anthrones, tannins, flavonoids, higher alcohols, phenols, steroids and essential oils. Since there is a growing awareness in correlating the phytochemical constituents of plants with their pharmacological activity, wild toxic plant can be one of the preferences for medicinal uses.

Keywords

• TLC
• matK
• rbcL
• wild toxic plant

References

1. Godofredo, S. (2015). Semecarpus cuneiformis Blanco. Philippine Medicinal Plant. Retrieved from http://www.stuartxchange.com/Ligas.html.
2. Bhatia, K., Kataria R., Singh A., Zainab HS. & Kumar R. (2014) Allergic Contact Dermatitis by Semecarpus anacardium for Evil Eye: A prospective study from Central India. Indian Journal of Basic and Applied Medical Research, 3(3) P-122-127.
3. Cobiac, L. (2006). Herbal Medicines: A guide for health-care professionals. Pharmaceutical Press, 28(1), 303-335.
4. Aguinaldo A.M., Espeso E.I., Guevara B.Q., and Nonato M. G. (2005). A Guidebook to Plant Screening: Phytochemical and Biological/ Beatrice Q. Guevara, ed. -Revised ed.- Manila: Research Center for the Natural Sciences, University of Sto Tomas.
5. Waksmundzka-Hajnos, M & M.A., Hawryl & Ciesla, Lukasz. (2014). Analysis of Plant Material. Instrumental Thin-Layer Chromatography, 505-554. doi.org/10.10166/B978-0-12-417223-4.00019-4.
6. Techen, N., Parveen, I., Pan, Z., Khan, I. (2014). DNA barcoding of medicinal plant material for identification. Current Opinion in Biotechnology, 25, 103-110.
7. Murray, M. G., and Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8(19), 4321-4326.\
8. Bele, A.A., & Khale, A. (2011) An overview on thin layer chromatography, IJPSR, 2(2), 2011, 256-267.

9. Low, T., (1991) Wild Foods of Australia, ISBN 0-207-16930-6
10. Pelser, P. (2012). Co’s Digital Flora of the Philippines; Anacardiaceae. Semecarpus cuneiformis Blanco Fl, Filip. Retrieved from www.philippineplants.org/Families/Anacardiaceae.html
11. Pell, K. S. (2004). Molecular systematics of the cashew family (Anacardiaceae). Plant Biology. Electronic Thesis and Dissertation. Retrieved from http://etd.lsu.edu/docs/available/etd-04152004-101232/unrestricted/Pell_dis.pdf
12. Mazimba, O., Wale, K., Kwape, T. E. Mihigo, S.O., Bokolo, M.K. (2015). Cinnamomum verum: ethylacetate and methanol extracts antioxidant and antimicrobial activity. Journal of Medicinal Plants Studies, 3(3), 28-32.
13. Talapatra, S.K., Talapatra, B. (2015). Chemistry of plant natural products: Stereochemistry, Conformation, Synthesis, Biology, and Medicine. Springer, Heidelberg, New York.
14. Hoffman, D. (2003). Medical Herbalism, the Science and Practice of Herbal Medicine. Healing Arts Press, 1, 303-360.