Cytotoxic and Apoptotic Activities of Rhizopogon roseolus (Corda) Th.Fr. Extracts

Abstract

Many species of mushrooms have been used since ancient times, especially in Asian countries, as a food supplement and in the medical field due to their different biological activities. Nowadays, especially in Japan, Korea and China, various mushroom extracts have been used as potential additives in chemotherapy and radiation treatments. In this study, anticancer activity and apoptotic effect of Rhizopogon roseolus were investigated. The methanol and water extracts of mushroom were tested against HL- 60 human cancer cell line. Antiproliferative effects of the extracts were evaluated by using MTT method and apoptosis and necrosis ratios of the cells treated with extracts were determined by using Hoechst/Propidium iodide (HO/PI) staining method. According to obtained data, antiprolifarative effect of the methanol extract was higher than water extract and this effect was a concentration depending manner. Both of the extracts were shown higher apoptotic effect than necrotic effect on the HL-60 cell line.

Keywords

• Rhizopogon roseolus
• Mushroom
• Apoptosis
• Cell culture
• HL-60

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