Year 2016, Volume 3 , Issue 1, Pages 3 - 13 2016-07-14

New Approaches for Effective Microwave Assisted Extraction of Caffeine and Catechins from Green Tea

Gönül SERDAR [1] , Ezgi DEMİR [2] , Serhat BAYRAK [3] , Münevver SÖKMEN [4]


This study was designed to develop an effective microwave assisted extraction (MAE) method for extraction of caffeine and catechins from green tea samples. A series of solvents (water, ethanol:water mixture or citric acid:water mixture) were used for extraction of green tea samples (fresh, frozen or dried) collected in three collection periods (first, second and third collection periods). Tea samples were extracted using water in a close microwave system under the certain extraction conditions. Extraction was carried out under a controlled 600 W microwave power at 80 oC temperature for 4 min irradiation time. Alternative to water, an ethanol-water mixture (1:1) or a citric acid-water (1:1) mixture was used as extracting solvent under the same conditions. After MAE crude aqueous extract was partitioned first with chloroform to separate caffeine then ethyl acetate for catechins. Both caffeine and catechin extraction was quite successful with microwave assisted system employing only 4 minutes treatment. The highest caffeine yield was obtained in the second collection period using frozen green tea samples and ethanol-water as extracting solvent. The yield of catechin extracts was between 0.84-3.96% depending on the solvent system used for extraction. Ethanol-water mixture seems to be appropriate for effective extraction in the basis of extract yields. However, HPLC results showed that individual catechin content of each extraction is more important criteria for the evaluation of most effective extraction medium rather than the mass of the extract. Using citric acid as extracting solvent in MAE seem to be more fruitful providing 100% catechin mixture with the highest EGCG content.
Caffeine extraction, catechin extraction, microwave assisted extraction
Subjects Biology
Other ID JA37PF85EC
Published Date January
Journal Section Articles
Authors

Author: Gönül SERDAR

Author: Ezgi DEMİR

Author: Serhat BAYRAK

Author: Münevver SÖKMEN

Dates

Publication Date : July 14, 2016

Bibtex @research article { ijsm240697, journal = {International Journal of Secondary Metabolite}, issn = {}, eissn = {2148-6905}, address = {Prof. Dr. İzzet KARA}, publisher = {İzzet KARA}, year = {2016}, volume = {3}, pages = {3 - 13}, doi = {10.21448/ijsm.240697}, title = {New Approaches for Effective Microwave Assisted Extraction of Caffeine and Catechins from Green Tea}, key = {cite}, author = {SERDAR, Gönül and DEMİR, Ezgi and BAYRAK, Serhat and SÖKMEN, Münevver} }
APA SERDAR, G , DEMİR, E , BAYRAK, S , SÖKMEN, M . (2016). New Approaches for Effective Microwave Assisted Extraction of Caffeine and Catechins from Green Tea. International Journal of Secondary Metabolite , 3 (1) , 3-13 . DOI: 10.21448/ijsm.240697
MLA SERDAR, G , DEMİR, E , BAYRAK, S , SÖKMEN, M . "New Approaches for Effective Microwave Assisted Extraction of Caffeine and Catechins from Green Tea". International Journal of Secondary Metabolite 3 (2016 ): 3-13 <https://dergipark.org.tr/en/pub/ijsm/issue/22523/240697>
Chicago SERDAR, G , DEMİR, E , BAYRAK, S , SÖKMEN, M . "New Approaches for Effective Microwave Assisted Extraction of Caffeine and Catechins from Green Tea". International Journal of Secondary Metabolite 3 (2016 ): 3-13
RIS TY - JOUR T1 - New Approaches for Effective Microwave Assisted Extraction of Caffeine and Catechins from Green Tea AU - Gönül SERDAR , Ezgi DEMİR , Serhat BAYRAK , Münevver SÖKMEN Y1 - 2016 PY - 2016 N1 - doi: 10.21448/ijsm.240697 DO - 10.21448/ijsm.240697 T2 - International Journal of Secondary Metabolite JF - Journal JO - JOR SP - 3 EP - 13 VL - 3 IS - 1 SN - -2148-6905 M3 - doi: 10.21448/ijsm.240697 UR - https://doi.org/10.21448/ijsm.240697 Y2 - 2015 ER -
EndNote %0 International Journal of Secondary Metabolite New Approaches for Effective Microwave Assisted Extraction of Caffeine and Catechins from Green Tea %A Gönül SERDAR , Ezgi DEMİR , Serhat BAYRAK , Münevver SÖKMEN %T New Approaches for Effective Microwave Assisted Extraction of Caffeine and Catechins from Green Tea %D 2016 %J International Journal of Secondary Metabolite %P -2148-6905 %V 3 %N 1 %R doi: 10.21448/ijsm.240697 %U 10.21448/ijsm.240697
ISNAD SERDAR, Gönül , DEMİR, Ezgi , BAYRAK, Serhat , SÖKMEN, Münevver . "New Approaches for Effective Microwave Assisted Extraction of Caffeine and Catechins from Green Tea". International Journal of Secondary Metabolite 3 / 1 (July 2016): 3-13 . https://doi.org/10.21448/ijsm.240697
AMA SERDAR G , DEMİR E , BAYRAK S , SÖKMEN M . New Approaches for Effective Microwave Assisted Extraction of Caffeine and Catechins from Green Tea. Int. J. Sec. Metabolite. 2016; 3(1): 3-13.
Vancouver SERDAR G , DEMİR E , BAYRAK S , SÖKMEN M . New Approaches for Effective Microwave Assisted Extraction of Caffeine and Catechins from Green Tea. International Journal of Secondary Metabolite. 2016; 3(1): 13-3.