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Determination of Genetic Polymorphism Among Soybean (Glycine max. L. Merrill) Cultivars Developed in Recent Years through RAPD Markers

Year 2015, Volume 2, Issue 1, 3 - 7, 14.01.2015

Abstract

Genotype or cultivar identification is the first step to ensure plant breeder’s rights. If registered cultivars or germplasms were not distinguished by any mechanism, then seed production or novel varieties could not be controlled. The RAPD markers are chosen to distinguish seven soybean ( Glycine max. L. Merrill ) cultivars from each other and to assign the polymorphisms among of them in this study. Eight primers were used and DNA fingerprinting of all cultivars were determined with that primers. According to that fingerprinting all cultivars were distingusihed from each other in DNA banding pattern. Using these markers results, similarity index in between soybean cultivars were calculated. Dwight and Maverick were the most similar cultivars ( These two cultivars were similar at 0,70 point to each other. ) In addition that Maverick and Defiance cultivars have shown the highest polymorphism rate ( These two cultivars were similar at 0,39 point each other. ).

References

  • Arumuganathan K. and Earle E.D., (1991). Nuclear DNA content of some important plant species. Plant Mol. Bio. Rep., 9: pp.-218.
  • Cregan P.B., Jaruik T., Bush A.L., Shoemaker R.C., Lark K.G., Kohler A.L., Kaya N.,Vantoai T.T., Lohnesd G., Chung J., and Specht J.E., (1999). An integrated genetic linkage map of soybean genome. Crop Sci., 39: pp.1464-1490.
  • Dellaporta S.L., Woods J. and Hicks J.B., (1983). A plant DNA mini preparation. Plant Mol. Biol. Rep., 1: pp.19-21.
  • Fehr W.R., (1987). Breeding methods for cultivar development. In B.E. Caldwell (Ed) Soybeans: Improvement, production, and uses. Agronomy, 16: pp.249-294.
  • Gizlice Z., Carter T.E. and Burton J.W., JR.,(1994). Genetic base for North American Public soybean cultivars released between 1947 and 1988. Crop Sci, 34: pp.1143-1151.
  • Hymowitz T. and Singh R.J., (1987). Taxomony and speciation. P.23-48. In RJ Wilcox (ed) Soybeans: Improvement, production. And uses. 2nd ed. Agron. Monogr. 16.
  • Markley S. K., (1950). Soybeans and soybean products. Volum 1. New york. USA. P. 15-210.
  • Özden Y., (2000). Buğday’da (Triticum aestivum L.) DNA parmakizi çalışmaları. G.Y.T.E. Yükseklisans Tezi. Gebze/KOCAELİ.
  • Özüdoğru E.A.,(2000). Arpa (Hordeum vulgare L.) döl analizlerinde genetik markırların kullanımı. G.Y.T.E. Yükseklisans Tezi. Gebze/KOCAELİ.
  • Rafalski J.A., and Tingey S.V., (1993). Genetic diagnostic in plant breeding: RAPDs, microsatellites and machines. Reviews. 9(8): pp.275-279.
  • Sneath H.A., and Sokal R.R.,(1973). Numerical Taxonomy. Freeman, San Francisco, USA.
  • Williams J.K.G., Rafalski J.A. and Tingey S.V., (1990a). Genetic analysis using RAPD markers. In: Methods in Enzymology. Academic Press, Orlando.

Determination of Genetic Polymorphism Among Soybean (Glycine max. L. Merrill) Cultivars Developed in Recent Years through RAPD Markers

Year 2015, Volume 2, Issue 1, 3 - 7, 14.01.2015

Abstract

Genotype or cultivar identification is the first step to ensure plant breeder’s rights. If registered cultivars or germplasms were not distinguished by any mechanism, then seed production or novel varieties could not be controlled. The RAPD markers are chosen to distinguish seven soybean ( Glycine max. L. Merrill ) cultivars from each other and to assign the polymorphisms among of them in this study. Eight primers were used and DNA fingerprinting of all cultivars were determined with that primers. According to that fingerprinting all cultivars were distingusihed from each other in DNA banding pattern. Using these markers results, similarity index in between soybean cultivars were calculated. Dwight and Maverick were the most similar cultivars ( These two cultivars were similar at 0,70 point to each other. ) In addition that Maverick and Defiance cultivars have shown the highest polymorphism rate ( These two cultivars were similar at 0,39 point each other. ).

References

  • Arumuganathan K. and Earle E.D., (1991). Nuclear DNA content of some important plant species. Plant Mol. Bio. Rep., 9: pp.-218.
  • Cregan P.B., Jaruik T., Bush A.L., Shoemaker R.C., Lark K.G., Kohler A.L., Kaya N.,Vantoai T.T., Lohnesd G., Chung J., and Specht J.E., (1999). An integrated genetic linkage map of soybean genome. Crop Sci., 39: pp.1464-1490.
  • Dellaporta S.L., Woods J. and Hicks J.B., (1983). A plant DNA mini preparation. Plant Mol. Biol. Rep., 1: pp.19-21.
  • Fehr W.R., (1987). Breeding methods for cultivar development. In B.E. Caldwell (Ed) Soybeans: Improvement, production, and uses. Agronomy, 16: pp.249-294.
  • Gizlice Z., Carter T.E. and Burton J.W., JR.,(1994). Genetic base for North American Public soybean cultivars released between 1947 and 1988. Crop Sci, 34: pp.1143-1151.
  • Hymowitz T. and Singh R.J., (1987). Taxomony and speciation. P.23-48. In RJ Wilcox (ed) Soybeans: Improvement, production. And uses. 2nd ed. Agron. Monogr. 16.
  • Markley S. K., (1950). Soybeans and soybean products. Volum 1. New york. USA. P. 15-210.
  • Özden Y., (2000). Buğday’da (Triticum aestivum L.) DNA parmakizi çalışmaları. G.Y.T.E. Yükseklisans Tezi. Gebze/KOCAELİ.
  • Özüdoğru E.A.,(2000). Arpa (Hordeum vulgare L.) döl analizlerinde genetik markırların kullanımı. G.Y.T.E. Yükseklisans Tezi. Gebze/KOCAELİ.
  • Rafalski J.A., and Tingey S.V., (1993). Genetic diagnostic in plant breeding: RAPDs, microsatellites and machines. Reviews. 9(8): pp.275-279.
  • Sneath H.A., and Sokal R.R.,(1973). Numerical Taxonomy. Freeman, San Francisco, USA.
  • Williams J.K.G., Rafalski J.A. and Tingey S.V., (1990a). Genetic analysis using RAPD markers. In: Methods in Enzymology. Academic Press, Orlando.

Details

Primary Language English
Subjects Biology
Other ID JA37RD96GK
Journal Section Articles
Authors

Mine PAKYÜREK This is me
SIIRT UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF HORTICULTURE


Abdülkadir AKÇİN This is me
Gebze Institute of Technology, Faculty of Science, Department of Molecular Biology and Genetics, Kocaeli, Turkey

Publication Date January 14, 2015
Published in Issue Year 2015, Volume 2, Issue 1

Cite

APA Pakyürek, M. & Akçin, A. (2015). Determination of Genetic Polymorphism Among Soybean (Glycine max. L. Merrill) Cultivars Developed in Recent Years through RAPD Markers . International Journal of Secondary Metabolite , 2 (1) , 3-7 . Retrieved from https://dergipark.org.tr/en/pub/ijsm/issue/22525/240710

International Journal of Secondary Metabolite (IJSM)

ISSN-e: 2148-6905