2,4-Dimetilpirol’ün Başak Yanıklığı Hastalığı Etmeni Fusarium culmorum’un Üzerine Etkilerinin İncelenmesi

Year 2017, Volume: 4 Issue: 2, 103 - 113, 01.07.2017

Özlem Sefer Emre Yörük Elif Sedef Develi Ayşe Server Sezer Zeynep Konukcu

https://doi.org/10.21448/ijsm.307943

Cited By: 5

Abstract

Bitki patojeni Fusarium
culmorum dünyada ve ülkemizde tahıllar üzerinde kök çürüklüğü ve başak yanıklığı
başta olmak üzere çeşitli hastalıklara neden olmaktadır. Bu çalışmada F. culmorum ile mücadele kullanılabilecek
yeni ve potansiyel bir ajan olarak 2,4-Dimetilpirol’ün patojen üzerindeki etkileri
incelenmiştir. Bu amaçla farklı konsantrasyonlarda 2,4-Dimetilpirol (0, 0.5, 1,
2 ve 4 mg mL^-1) uygulanmış F. culmorum
20F izolatında artan konsantrasyonlarda doğrusal büyüme oranındaki (DBO)  değişim incelenmiş ve kontrol ve deney grupları
arasında anlamlı bir düşüş olduğu görülmüştür. 
Ayrıca seksüel üreme ve hücre çeperi bütünlüğünden sorumlu Mgv1 geninin anlatımı gerçek zamanlı kantitatif
polimeraz zincir reaksiyonu (k-PZR) ve ters transkripsiyon PZR (RT- PZR) ile incelenmiştir.
Deney gruplarında kontrol gruplarına göre Mgv1
geni için 5.21±0.05x10² kat artma saptanmıştır. Elde edilen bulgulara
göre 2,4-Dimetilpirol’ün, F. culmorum türünde
potansiyel bir antifungal etkili ajan olabileceği gösterilmiştir. İleriki çalışmalarda
bu ajanın tarladaki fungal biyokütlenin ve toksin üretiminin azaltılmasını sağlayarak
hastalık ile mücadelede yeni bir yaklaşım olarak kullanılabileceği düşünülmektedir.

Keywords

Antifungal, Başak Yanıklığı, Fusarium, 2.4-Dimetilpirol

Investigation of the Effects of 2,4-Dimethylpyrrole on Fusarium culmorum Causal Agent of Head Blight Disease

Abstract

Fusarium culmorum, a phytopathogen, cause several
diseases including root rot and head blight on cereals in worldwide and our country.
In this study, effects of 2,4-Dimethylpyrrole, as a novel and potential agent on
pathogen which can be used in fight with F. culmorum were investigated. For this
purpose, alteration in linear growth rate (LGR) in F. culmorum 20F isolate, subjected to different concentrations of 2,4-Dimethylpyrrole
(0, 0.5, 1, 2, 4 mg mL^-1) was investigated and the significant decrase
between control and experiment sets was determined. Besides, expression of Mgv1 gene, responsible for sexual stage and
cell wall integrity, was investigated via quantitative real time polymerase chain
reaction (q-PCR) and reverse transcription PCR (RT-PCR). In comparison to control
groups, 5.21±0.05x10² fold increases in experiment set for Mgv1 gene was detected. According to findings
obtained, it was shown that 2,4-Dimethylpyrrole could be a potential antifungal
agent for F. culmorum. In further works,
it is suggested that this agent could be used as a novel approach in disease control
including inhibition of fungal biomass and toxin production in field.

Keywords

Antifungal, Head Blight, Fusarium, 2.4-Dimethylpyrrole

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