Extraction, purification, antioxidant properties and stability conditions of phytomelanin pigment on the sunflower seeds

Year 2018, Volume: 5 Issue: 2, 140 - 148, 05.07.2018

Yuksel Keles , Önder Özdemir

https://doi.org/10.21448/ijsm.377470

Cited By: 11

Abstract

Phytomelanin
pigment, a rare secondary matabolite in plants, has potential for use in the
cosmetic and food sectors. This pigment has antioxidant, antimicrobial and
ultraviolet (UV) light absorbing properties, so it can be used as natural food
coloring and in the field of cosmetics. In this study, extraction,
purification, antioxidant properties and stability conditions of phytomelanin
pigment found in sunflower (Helianthus
annuus L.) seed coat were investigated. NaOH, KOH and NH₄OH
solutions were tested at different concentrations for extraction. It has been
determined that the most suitable solvent for extraction is 0.3 M NaOH. The
purification process involves precipitation with HCl followed by washing with
ethyl alcohol, ethyl acetate and acetone. The findings show that the proportion
of phytomelanin in sunflower seeds is 1.95% and that the antioxidant capacity
is 9.8% ascorbic acid equivalent. The purity degree of the purified
phytomelanin pigment and that of the synthetic phytomelanin pigment were
compared by thin layer chromatography (TLC). Chromatography findings have shown
that the purification performance is quite high. It was determined that the
pigment was slowly deteriorated in temperature and light, and was not affected
by air. Consequently, sunflower seed coat can be a convenient and economical
source of producing pure phytomelanin for industrial use.

Keywords

Helianthus annuus, rare pigments, seconder metabolite, seed coat, TLC

Extraction, purification, antioxidant properties and stability conditions of phytomelanin pigment on the sunflower seeds

Abstract

Phytomelanin pigment, a rare secondary matabolite in plants, has potential for use in the cosmetic and food sectors. This pigment has antioxidant, antimicrobial and ultraviolet (UV) light absorbing properties, so it can be used as natural food coloring and in the field of cosmetics. In this study, extraction, purification, antioxidant properties and stability conditions of phytomelanin pigment found in sunflower (Helianthus annuus L.) seed coat were investigated. NaOH, KOH and NH4OH solutions were tested at different concentrations for extraction. It has been determined that the most suitable solvent for extraction is 0.3 M NaOH. The purification process involves precipitation with HCl followed by washing with ethyl alcohol, ethyl acetate and acetone. The findings show that the proportion of phytomelanin in sunflower seeds is 1.95% and that the antioxidant capacity is 9.8% ascorbic acid equivalent. The purity degree of the purified phytomelanin pigment and that of the synthetic phytomelanin pigment were compared by thin layer chromatography (TLC). Chromatography findings have shown that the purification performance is quite high. It was determined that the pigment was slowly deteriorated in temperature and light, and was not affected by air. Consequently, sunflower seed coat can be a convenient and economical source of producing pure phytomelanin for industrial use.

Keywords

Helianthus annuus, Rare pigments, Seconder metabolite, Seed coat, TLC

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