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Transgenerational Transmission of Radiation-Induced Expression Patterns of Arabidopsis Thaliana (L.) Heynh. Rad51 and Rad1 Genes

Year 2018, Volume: 5 Issue: 2, 149 - 155, 05.07.2018
https://doi.org/10.21448/ijsm.415191

Abstract

Transcription rates of the genes AtKu70, AtRAD51, AtRad1, involved in maintaining Arabidopsis thaliana genome stability, in relation to the modification of phenotypic characteristics in irradiated plants and their progeny after the action of acute and fractionated X-ray radiation were studied. Differences in the transcription rate were measured by densitometric analysis of cDNA, synthesized by reverse transcription at the template of mRNAs, extracted from fresh leaves after 2 hours irradiation treatment. The doses 3 Gy, 12 Gy, 15 Gy and 21 Gy with 1.48 Gy/s specific dose rate were applied. Significant correlation between phenotype modifications in F0 and F1 generations, between phenotype traits and caretaker genes activity in irradiated F0 plants were shown. Also preservation of changes in the pattern of AtRad1 and AtRAD51 but not AtKu70 expression in F1 plant leaves had been revealed. Changes in F1 compared with F0 generation do not correspond to the extrapolation of dependence between the phenotypic modifications and DNA repair genes transcription rate in the leaves of irradiated plants. Based on the obtained data it could be suggested that the altered transcriptional activity of AtRAD51 and AtRad1 reflects the transfer of DNA lesions from parent to offspring.

References

  • Mannuss, A., Trapp, O., Puchta, H. (2012). Gene Regulation in Response to DNA Damage, Biochimica et Biophysica Acta, 1819(2), 154-165. http://dx.doi.org/10.1016/j.bbagrm.2011.08.003
  • Vannier, J.-B., Depeiges, A., White, C., Gallego, M.E. (2009). ERCC1/XPF Protects Short Telomeres from Homologous Recombination in Arabidopsis thaliana, PLOS Genetics, 5(2), e1000380. http://dx.doi.org/10.1371/journal.pgen.1000380
  • Yoshiyama, K., Sakaguchi, K., Kimura, S. (2013). DNA Damage Response in Plants: Conserved and Variable Response Compared to Animals, Biology, 2 (4), 1338-1356. http://dx.doi.org/10.3390/biology2041338
  • Boulton, S., Jackson, S. (1998) Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencing, The EMBO Journal, 17(6), 1819-1828. http://dx.doi.org/10.1093/emboj/17.6.1819
  • Frankenberg-Schwager, M., Gebauer, A., Koppe, C., Wolf, H., Pralle, E., Frankenberg, D. (2009). Single-strand annealing, conservative homologous recombination, nonhomologous DNA end joining, and the cell cycle-dependent repair of DNA double-strand breaks induced by sparsely or densely ionizing radiation, Radiation Research, 171(3), 265-273. http://dx.doi.org/10.1667/RR0784.1
  • Lieber, M.R. (2010). NHEJ and Its Backup Pathways: Relation to Chromosomal Translocations, Nature Structural & Molecular Biology, 17(4), 393-395. http://dx.doi.org/10.1038/nsmb0410-393
  • Waterworth, W., Drury, G., Bray, C., West, C. (2011). Repairing Breaks in the Plant Genome: The Importance of Keeping It Together, New Phytologist, 192 (4), 805-822. http://dx.doi.org/10.1111/j.1469-8137.2011.03926.x
  • Li, J., Harper, L.C., Golubovskaya, I., Wang, C.R., Weber, D., Meeley, R.B., McElver, J., Bowen, B., Cande, W.Z., Schnable, P.S. (2007). Functional Analysis of Maize RAD51 in Meiosis and Double–Strand Break Repair, Genetics, 176(3), 1469-1482. http://dx.doi.org/10.1534/genetics.106.062604
  • Ma, W., Westmoreland J. W., Resnick M. A. (2013). Homologous recombination rescues ssDNA gaps generated by nucleotide excision repair and reduced translesion DNA synthesis in yeast G2 cells, PNAS, 110(31), 2895-2904. http://dx.doi.org/10.1073/pnas.1301676110
  • Besse, B., Olaussen, K.A., Soria, J.C. (2013). ERCC1 and RRM1: ready for prime time?, Journal of Clinical Oncology, 31(8), 1050-1060. http://dx.doi.org/10.1200/JCO.2012.43.0900
  • Hwang, J.-Y., Smith, S., & Myung, K. (2005). The Rad1-Rad10 Complex Promotes the Production of Gross Chromosomal Rearrangements from Spontaneous DNA Damage in Saccharomyces cerevisiae, Genetics, 169(4), 1927-1937. http://dx.doi.org/10.1534/genetics.104.039768
  • Ma, J.L., Kim, E.M., Haber, J.E., Lee, S.E. (2003). Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequences, Molecular and Cellular Biology, 23(23), 8820-8828. http://dx.doi.org/10.1128/MCB.23.23.8820-8828.2003
  • McVey, M., Lee, S.E. (2008). MMEJ repair of double-strand breaks (director's cut): deleted sequences and alternative endings, Trends in Genetics, 24(11), 529-538. http://dx.doi.org/10.1016/j.tig.2008.08.007
  • Scuric, Z., Chan, C.Y., Hafer, K., Schiestl, R.H. (2009). Ionizing Radiation Induces Microhomology-Mediated End Joining in trans in Yeast and Mammalian Cells, Radiation Research, 171(4), 454-463. http://dx.doi.org/10.1667/RR1329.1
  • Zhang, Y., Rohde, L.H., Wu, H. (2009). Involvement of nucleotide excision and mismatch repair mechanisms in double strand break repair, Current Genomics, 10(4), 250-258. http://dx.doi.org/10.2174/138920209788488544
  • Litvinov, S., Rashydov, N. (2017) The transcriptional response of Arabidopsis thaliana L. AtKu70, AtRAD51 and AtRad1 genes to X-rays, Journal of Agricultural Science and Technology A, 7 (1), 52-60. http://dx.doi.org/10.17265/2161-6256/2017.01.008
  • Bradford, W., Cahoon, L., Freel, S., Mays Hoopes, L., Eckdahl, T. (2005) An Inexpensive Gel Electrophoresis-Based Polymerase Chain Reaction Method for Quantifying mRNA Levels, Cell Biology Education, 4(2), 157-168. http://dx.doi.org/10.1187/cbe.04-09-0051
  • Litvinov, S. (2014). Effects of Chronic Exposure of Seeds and Seedlings of Arabidopsis thaliana by Low Doses of γ-Radiation on Plant Growth and Development, Nuclear Physics and Atomic Energy, 15(4), 406-414.

Transgenerational Transmission of Radiation-Induced Expression Patterns of Arabidopsis Thaliana (L.) Heynh. Rad51 and Rad1 Genes

Year 2018, Volume: 5 Issue: 2, 149 - 155, 05.07.2018
https://doi.org/10.21448/ijsm.415191

Abstract

Transcription
rates of the genes AtKu70, AtRAD51, AtRad1, involved in maintaining Arabidopsis
thaliana
genome stability, in relation to the modification of phenotypic
characteristics in irradiated plants and their progeny after the action of
acute and fractionated X-ray radiation were studied. Differences in the
transcription rate were measured by densitometric analysis of cDNA, synthesized
by reverse transcription at the template of mRNAs, extracted from fresh leaves
after 2 hours irradiation treatment. The doses 3 Gy, 12 Gy, 15 Gy and 21 Gy
with 1.48 Gy/s specific dose rate were applied. Significant correlation between
phenotype modifications in F0 and F1 generations, between
phenotype traits and caretaker genes activity in irradiated F0
plants were shown. Also preservation of changes in the pattern of AtRad1 and AtRAD51 but not AtKu70
expression in F1 plant leaves had been revealed. Changes in F1
compared with F0 generation do not correspond to the extrapolation
of dependence between the phenotypic modifications and DNA repair genes
transcription rate in the leaves of irradiated plants. Based on the obtained
data it could be suggested that the altered transcriptional activity of AtRAD51 and AtRad1 reflects the transfer of DNA lesions from parent to
offspring.

References

  • Mannuss, A., Trapp, O., Puchta, H. (2012). Gene Regulation in Response to DNA Damage, Biochimica et Biophysica Acta, 1819(2), 154-165. http://dx.doi.org/10.1016/j.bbagrm.2011.08.003
  • Vannier, J.-B., Depeiges, A., White, C., Gallego, M.E. (2009). ERCC1/XPF Protects Short Telomeres from Homologous Recombination in Arabidopsis thaliana, PLOS Genetics, 5(2), e1000380. http://dx.doi.org/10.1371/journal.pgen.1000380
  • Yoshiyama, K., Sakaguchi, K., Kimura, S. (2013). DNA Damage Response in Plants: Conserved and Variable Response Compared to Animals, Biology, 2 (4), 1338-1356. http://dx.doi.org/10.3390/biology2041338
  • Boulton, S., Jackson, S. (1998) Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencing, The EMBO Journal, 17(6), 1819-1828. http://dx.doi.org/10.1093/emboj/17.6.1819
  • Frankenberg-Schwager, M., Gebauer, A., Koppe, C., Wolf, H., Pralle, E., Frankenberg, D. (2009). Single-strand annealing, conservative homologous recombination, nonhomologous DNA end joining, and the cell cycle-dependent repair of DNA double-strand breaks induced by sparsely or densely ionizing radiation, Radiation Research, 171(3), 265-273. http://dx.doi.org/10.1667/RR0784.1
  • Lieber, M.R. (2010). NHEJ and Its Backup Pathways: Relation to Chromosomal Translocations, Nature Structural & Molecular Biology, 17(4), 393-395. http://dx.doi.org/10.1038/nsmb0410-393
  • Waterworth, W., Drury, G., Bray, C., West, C. (2011). Repairing Breaks in the Plant Genome: The Importance of Keeping It Together, New Phytologist, 192 (4), 805-822. http://dx.doi.org/10.1111/j.1469-8137.2011.03926.x
  • Li, J., Harper, L.C., Golubovskaya, I., Wang, C.R., Weber, D., Meeley, R.B., McElver, J., Bowen, B., Cande, W.Z., Schnable, P.S. (2007). Functional Analysis of Maize RAD51 in Meiosis and Double–Strand Break Repair, Genetics, 176(3), 1469-1482. http://dx.doi.org/10.1534/genetics.106.062604
  • Ma, W., Westmoreland J. W., Resnick M. A. (2013). Homologous recombination rescues ssDNA gaps generated by nucleotide excision repair and reduced translesion DNA synthesis in yeast G2 cells, PNAS, 110(31), 2895-2904. http://dx.doi.org/10.1073/pnas.1301676110
  • Besse, B., Olaussen, K.A., Soria, J.C. (2013). ERCC1 and RRM1: ready for prime time?, Journal of Clinical Oncology, 31(8), 1050-1060. http://dx.doi.org/10.1200/JCO.2012.43.0900
  • Hwang, J.-Y., Smith, S., & Myung, K. (2005). The Rad1-Rad10 Complex Promotes the Production of Gross Chromosomal Rearrangements from Spontaneous DNA Damage in Saccharomyces cerevisiae, Genetics, 169(4), 1927-1937. http://dx.doi.org/10.1534/genetics.104.039768
  • Ma, J.L., Kim, E.M., Haber, J.E., Lee, S.E. (2003). Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequences, Molecular and Cellular Biology, 23(23), 8820-8828. http://dx.doi.org/10.1128/MCB.23.23.8820-8828.2003
  • McVey, M., Lee, S.E. (2008). MMEJ repair of double-strand breaks (director's cut): deleted sequences and alternative endings, Trends in Genetics, 24(11), 529-538. http://dx.doi.org/10.1016/j.tig.2008.08.007
  • Scuric, Z., Chan, C.Y., Hafer, K., Schiestl, R.H. (2009). Ionizing Radiation Induces Microhomology-Mediated End Joining in trans in Yeast and Mammalian Cells, Radiation Research, 171(4), 454-463. http://dx.doi.org/10.1667/RR1329.1
  • Zhang, Y., Rohde, L.H., Wu, H. (2009). Involvement of nucleotide excision and mismatch repair mechanisms in double strand break repair, Current Genomics, 10(4), 250-258. http://dx.doi.org/10.2174/138920209788488544
  • Litvinov, S., Rashydov, N. (2017) The transcriptional response of Arabidopsis thaliana L. AtKu70, AtRAD51 and AtRad1 genes to X-rays, Journal of Agricultural Science and Technology A, 7 (1), 52-60. http://dx.doi.org/10.17265/2161-6256/2017.01.008
  • Bradford, W., Cahoon, L., Freel, S., Mays Hoopes, L., Eckdahl, T. (2005) An Inexpensive Gel Electrophoresis-Based Polymerase Chain Reaction Method for Quantifying mRNA Levels, Cell Biology Education, 4(2), 157-168. http://dx.doi.org/10.1187/cbe.04-09-0051
  • Litvinov, S. (2014). Effects of Chronic Exposure of Seeds and Seedlings of Arabidopsis thaliana by Low Doses of γ-Radiation on Plant Growth and Development, Nuclear Physics and Atomic Energy, 15(4), 406-414.
There are 18 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Sergey Litvinov This is me

Namik Rashydov

Publication Date July 5, 2018
Submission Date January 15, 2018
Published in Issue Year 2018 Volume: 5 Issue: 2

Cite

APA Litvinov, S., & Rashydov, N. (2018). Transgenerational Transmission of Radiation-Induced Expression Patterns of Arabidopsis Thaliana (L.) Heynh. Rad51 and Rad1 Genes. International Journal of Secondary Metabolite, 5(2), 149-155. https://doi.org/10.21448/ijsm.415191
International Journal of Secondary Metabolite

e-ISSN: 2148-6905