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Analysis of Phytochemical Composition and Biological Activities of Verbascum cheiranthifolium var. cheiranthifolium stem and flowers

Year 2018, Volume 5, Issue 3, 233 - 242, 15.09.2018
https://doi.org/10.21448/ijsm.401127

Abstract

Within this study phytochemical composition, antioxidant and enzyme inhibitory activities of extracts obtained from stem and flower of Verbascum cheiranthifolium var. cheiranthifolium were analysed. Both of the extracts were detected as rich sources of phenolics (verbascoside and luteolin hexoside), various volatile and fatty acid compounds. Luteolin hexoside rich stem extract had pronounced FCR, FRAP and α-glucosidase inhibitory activities. Flower extract had high levels of ORAC assay and effectively suppressed activity of pancreatic lipase enzyme, which was rich in verbascoside compound. Phenolic compounds and volatile compounds present in the extracts might be the main contributors of antioxidant capacity and enzyme inhibitory activities of the stem and flower extracts. Pronounced antioxidant and enzyme inhibitory activities and rich bioactive composition determined in this study reveal that Verbascum cheiranthifolium var. cheiranthifolum extracts might be a good source for natural health attributing sources.

References

  • Tatlı, İ.İ., Akdemir, Z.Ş. (2004). Chemical constituents of Verbascum L. species, FABAD Journal of Pharmaceutical Sciences, 29, 93-107.
  • Mükemre, M., Behçet, L., Çakılcıoğlu, U. (2015). Ethnobotanical study on medicinal plants in villages of Çatak (Van-Turkey), Journal of Ethnopharmacology, 166, 361-374.
  • Dalar, A., Guo, Y., Konczak, I. (2014). Phenolic composition and potential anti inflammatory properties of Verbascum cheiranthifolium var. cheiranthifolium leaf, Journal of Herbal Medicine, 4(4), 195-200.
  • Tatlı, İ.İ., Akdemir, Z.Ş. (2006). Traditional uses and biological activities of Verbascum species, FABAD Journal of Pharmaceutical Sciences, 31, 85-96.
  • Küçük, S., Özdemir, F., İşcan, G., İncesu, Z. (2016) Determination of Cytotoxic and Anticandidal Activities of Three Verbascum L. Species from Turkey: V. cheiranthifolium Boiss. var. asperulum (Boiss.) Murb. Monorg., V. pycnostachyum Boiss. & Heldr and V. orgyale Boiss. & Heldr., Turkish Journal of Pharmaceutical Sciences, 13(3), 318-322.
  • Dalar, A., Konczak, I. (2013). Phenolic contents, antioxidant capacities and inhibitory activities against key metabolic syndrome relevant enzymes of herbal teas from Eastern Anatolia, Industrial Crops and Products, 44, 383-390.
  • Uzun, Y., Dalar, A., Konczak, I. (2017). Sempervivum davisii: phytochemical composition, antioxidant and lipase-inhibitory activities, Pharmaceutical Biology, 55(1), 532-540.
  • Shahidi F., Zhong Y. (2015). Measurement of antioxidant activity, Journal of Functional Foods, 18, 757-781.
  • Tadera, K., Minami, Y., Takamatsu, K., Matsuoka, T. (2006). Inhibition of α-glucosidase and α-amylase by flavonoids, Journal of Nutritional Science and Vitaminology, 52(2), 149-153.
  • Aldini, G., Piccoli, A., Beretta, G., Morazzoni, P., Riva, A., Marinello, C., Facino, R.M. (2006). Antioxidant activity of polyphenols from solid olive residues of cv Coratina, Fitoterapia, 77(2), 121-128.
  • Piné, R., Herranz-López, M., Funes, L., Borrás-Linares, I., Micol, V., Segura-Carretero, A., Fernández- Gutiérrez, A. (2013). Phenylpropanoids and their metabolites are the major compounds responsible for blood-cell protection against oxidative stress after administration of Lippia citriodora in rats, Phytomedicine, 20(12), 1112-1118.
  • Wong, I.Y.F., He, Z.D., Huang, Y., Chen, Z.Y. (2001). Antioxidative activities of phenylethanoid glycosides from Ligustrum purpurascens, Journal of Agricultural and Food Chemistry, 49, 3113–3119.
  • Chen, C.H., Lin, Y.S., Chien, M.Y., Hou, W.C., Hu, M.L. (2012). Antioxidant and antihypertensive activities of acteoside and its analogs. Botanical Studies, 53, 421–429.
  • Koo, K.A., Kim, S.H., Oh, T.H., Kim, Y.C. (2006). Acteoside and its aglycones protect primary cultures of rat cortical cells from glutamate-induced excitotoxicity. Life Sciences, 79, 709–716.
  • Manju, V., Balasubramaniyan, V., Nalini, N. (2005). Rat colonic lipid peroxidation and antioxidant status: the effect of dietary luteolin on 1, 2-dimethylhydrazine challenge, Cellular and Molecular Biology Letters, 10, 535-551.
  • Chen, C.Y., Peng, W.H., Tsai, K.D., Hsu, S.L. (2007). Luteolin supresses inflammation-associated gene expression by blocking NF-kappaB and AP-1 activation pathway in mouse alveolar macrophages, Life Sciences, 81, 1602-1614.
  • López-Lázaro, M. (2009). Distribution and Biological Activities of the Flavonoid Luteolin, Mini Reviews in Medicinal Chemistry, 9, 31-59.
  • Ruberto, J., Baratta, M.T. (2000). Antioxidant activity of selected essential oil components in two lipid model systems, Food Chemistry, 69, 167−74.

Analysis of Phytochemical Composition and Biological Activities of Verbascum cheiranthifolium var. cheiranthifolium stem and flowers

Year 2018, Volume 5, Issue 3, 233 - 242, 15.09.2018
https://doi.org/10.21448/ijsm.401127

Abstract

Within this study phytochemical composition, antioxidant and enzyme inhibitory activities of extracts obtained from stem and flower of Verbascum cheiranthifolium var. cheiranthifolium were analysed. Both of the extracts were detected as rich sources of phenolics (verbascoside and luteolin hexoside), various volatile and fatty acid compounds. Luteolin hexoside rich stem extract had pronounced FCR, FRAP and α-glucosidase inhibitory activities. Flower extract had high levels of ORAC assay and effectively suppressed activity of pancreatic lipase enzyme, which was rich in verbascoside compound. Phenolic compounds and volatile compounds present in the extracts might be the main contributors of antioxidant capacity and enzyme inhibitory activities of the stem and flower extracts. Pronounced antioxidant and enzyme inhibitory activities and rich bioactive composition determined in this study reveal that Verbascum cheiranthifolium var. cheiranthifolum extracts might be a good source for natural health attributing sources.

References

  • Tatlı, İ.İ., Akdemir, Z.Ş. (2004). Chemical constituents of Verbascum L. species, FABAD Journal of Pharmaceutical Sciences, 29, 93-107.
  • Mükemre, M., Behçet, L., Çakılcıoğlu, U. (2015). Ethnobotanical study on medicinal plants in villages of Çatak (Van-Turkey), Journal of Ethnopharmacology, 166, 361-374.
  • Dalar, A., Guo, Y., Konczak, I. (2014). Phenolic composition and potential anti inflammatory properties of Verbascum cheiranthifolium var. cheiranthifolium leaf, Journal of Herbal Medicine, 4(4), 195-200.
  • Tatlı, İ.İ., Akdemir, Z.Ş. (2006). Traditional uses and biological activities of Verbascum species, FABAD Journal of Pharmaceutical Sciences, 31, 85-96.
  • Küçük, S., Özdemir, F., İşcan, G., İncesu, Z. (2016) Determination of Cytotoxic and Anticandidal Activities of Three Verbascum L. Species from Turkey: V. cheiranthifolium Boiss. var. asperulum (Boiss.) Murb. Monorg., V. pycnostachyum Boiss. & Heldr and V. orgyale Boiss. & Heldr., Turkish Journal of Pharmaceutical Sciences, 13(3), 318-322.
  • Dalar, A., Konczak, I. (2013). Phenolic contents, antioxidant capacities and inhibitory activities against key metabolic syndrome relevant enzymes of herbal teas from Eastern Anatolia, Industrial Crops and Products, 44, 383-390.
  • Uzun, Y., Dalar, A., Konczak, I. (2017). Sempervivum davisii: phytochemical composition, antioxidant and lipase-inhibitory activities, Pharmaceutical Biology, 55(1), 532-540.
  • Shahidi F., Zhong Y. (2015). Measurement of antioxidant activity, Journal of Functional Foods, 18, 757-781.
  • Tadera, K., Minami, Y., Takamatsu, K., Matsuoka, T. (2006). Inhibition of α-glucosidase and α-amylase by flavonoids, Journal of Nutritional Science and Vitaminology, 52(2), 149-153.
  • Aldini, G., Piccoli, A., Beretta, G., Morazzoni, P., Riva, A., Marinello, C., Facino, R.M. (2006). Antioxidant activity of polyphenols from solid olive residues of cv Coratina, Fitoterapia, 77(2), 121-128.
  • Piné, R., Herranz-López, M., Funes, L., Borrás-Linares, I., Micol, V., Segura-Carretero, A., Fernández- Gutiérrez, A. (2013). Phenylpropanoids and their metabolites are the major compounds responsible for blood-cell protection against oxidative stress after administration of Lippia citriodora in rats, Phytomedicine, 20(12), 1112-1118.
  • Wong, I.Y.F., He, Z.D., Huang, Y., Chen, Z.Y. (2001). Antioxidative activities of phenylethanoid glycosides from Ligustrum purpurascens, Journal of Agricultural and Food Chemistry, 49, 3113–3119.
  • Chen, C.H., Lin, Y.S., Chien, M.Y., Hou, W.C., Hu, M.L. (2012). Antioxidant and antihypertensive activities of acteoside and its analogs. Botanical Studies, 53, 421–429.
  • Koo, K.A., Kim, S.H., Oh, T.H., Kim, Y.C. (2006). Acteoside and its aglycones protect primary cultures of rat cortical cells from glutamate-induced excitotoxicity. Life Sciences, 79, 709–716.
  • Manju, V., Balasubramaniyan, V., Nalini, N. (2005). Rat colonic lipid peroxidation and antioxidant status: the effect of dietary luteolin on 1, 2-dimethylhydrazine challenge, Cellular and Molecular Biology Letters, 10, 535-551.
  • Chen, C.Y., Peng, W.H., Tsai, K.D., Hsu, S.L. (2007). Luteolin supresses inflammation-associated gene expression by blocking NF-kappaB and AP-1 activation pathway in mouse alveolar macrophages, Life Sciences, 81, 1602-1614.
  • López-Lázaro, M. (2009). Distribution and Biological Activities of the Flavonoid Luteolin, Mini Reviews in Medicinal Chemistry, 9, 31-59.
  • Ruberto, J., Baratta, M.T. (2000). Antioxidant activity of selected essential oil components in two lipid model systems, Food Chemistry, 69, 167−74.

Details

Primary Language English
Subjects Biology
Published Date September
Journal Section Articles
Authors

Abdullah DALAR (Primary Author)
Van Yuzuncu Yil University
0000-0002-0080-2519
Türkiye


Aydin Sukru BENGU This is me
University of Bingol
Türkiye


Oruc ALLAHVERDİYEV This is me
Van Yuzuncu Yil University
Türkiye

Publication Date September 15, 2018
Published in Issue Year 2018, Volume 5, Issue 3

Cite

APA Dalar, A. , Bengu, A. S. & Allahverdiyev, O. (2018). Analysis of Phytochemical Composition and Biological Activities of Verbascum cheiranthifolium var. cheiranthifolium stem and flowers . International Journal of Secondary Metabolite , 5 (3) , 233-242 . DOI: 10.21448/ijsm.401127

International Journal of Secondary Metabolite (IJSM)

ISSN-e: 2148-6905