Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures

Year 2018, Volume: 5 Issue: 3, 210 - 216, 15.09.2018

Özlem Aras Aşci Tunhan Demirci , Nilgün Göktürk Baydar

https://doi.org/10.21448/ijsm.453016

Cited By: 2

Abstract

Madder (Rubia tinctorum L.) is a valuable plant rich in anthraquinones having dying properties and biological activities. This study was carried out to determine the effect of sodium chloride (NaCl) applications on the root growth and secondary metabolite accumulation in adventitious roots of madder. For this aim, adventitious roots derived from stem explants in in vitro conditions were cultured in MS medium containing different concentrations of NaCl (0, 1, 2, 3 and 4 g/l) for 7 days. Then roots were evaluated in terms of root growth index, total AQ, alizarin, purpurin and total phenolic contents. Based on the results, root growth decreased in line with the elevating level of NaCl while secondary metabolite accumulation significantly increased with NaCl applications compared to the controls. It was determined that NaCl at 3 g/l concentration was the most effective application in terms of total AQ, alizarin, purpurin and phenolic accumulation.

Keywords

Rubia tinctorum, root culture, in vitro, sodium chloride, secondary metabolite

Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures

Abstract

Madder
(Rubia tinctorum L.) is a valuable
plant rich in anthraquinones having dying properties and biological activities.
This study was carried out to determine the effect of sodium chloride (NaCl) applications
on the root growth and secondary metabolite accumulation in adventitious roots
of madder. For this aim, adventitious roots derived from stem explants in in vitro conditions were cultured in MS
medium containing different concentrations of NaCl (0, 1, 2, 3 and 4 g/l) for 7
days. Then roots were evaluated in terms of root growth index, total AQ,
alizarin, purpurin and total phenolic contents. Based on the results, root
growth decreased in line with the elevating level of NaCl while secondary
metabolite accumulation significantly increased with NaCl applications compared
to the controls. It was determined that NaCl at 3 g/l concentration was the
most effective application in terms of total AQ, alizarin, purpurin and
phenolic accumulation.

Keywords

Rubia tinctorum, root culture, in vitro, sodium chloride, secondary metabolite

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