Research Article
BibTex RIS Cite

Electromagnetic radiation of low intensity as a factor of change of phenolic compounds content

Year 2018, Volume: 5 Issue: 3, 252 - 258, 15.09.2018
https://doi.org/10.21448/ijsm.459102

Abstract

The influence of low-intensity electromagnetic radiation (EMR) on the content of phenolic compounds (PC) in plants on different stages of ontogeny is discussed. A medicinal plant (Calendula officinalis L.) and agricultural crop Fagopyrum sagittatum G., diploid varieties and tetraploid varieties were chosen as objects of study. Microwave EMR in two frequency bands, 53.57-78.33 GHz (wide range mode) and 64.00-66.00 GHz (narrow range mode), has been selected to learn the physical effects on seeds, with a treatment exposure time of 20, 12, or 8 min. The experiments were conducted under laboratory and field conditions. Treated and control seeds were germinated by 100 pcs in a growth chamber on moistened filter paper at 22–24˚C in the dark and placed on the daylight from the third day. The level of PC in the seedlings was measured with the Folin-Ciocalteu reagent. It was found that in the EMR pretreated C. officinalis seedlings the level of PC is maximal on 14th day of germination. The wide mode treatment for 12 min results in a two-fold increase in the level of PC in juvenile shoots with respect to control. Different constitutional levels of phenolic compounds in the leaves of diploid and tetraploid varieties of F. sagittatum in the most critical phases of ontogeny were found. It is recommended to use EMR with a 12 min exposure time for C. officinalis. The narrow frequency mode with an exposure time of 20 min is preferable to use for most varieties of F. sagittatum.

References

  • Zaprometov, M.N. (1993). Physiology and biochemistry of flavonoids. J. Physiology of plants, 40(6), 921–931. (in Russian)
  • Gorgun, Y., Mazets, Z., Shysh, S., Elovskaya, N. (2017). Influence of low-intensity electromagnetic radiation on the dynamics of accumulation of phenolic compounds in buckwheat plants tetraploid. «Modern Problems of Natural Science in Science and the Educational Process» Materials of the Republican Scientific and Practical Conference. Minsk (in Russian).
  • Jambazishvili, S.N. (1974). Presowing treatment of seeds of sugar beet by various electrophysical methods and their influence on productivity and quality of root crops: the dissertation author's abstract the candidate of agricultural sciences: 06.01.09, Tbilisi (in Russian)
  • Novikova, G.V. (1980). Substantiation and research of technological process of influence of EMF microwave on seeds of vegetable cultures: the dissertation of the candidate tehn. sciences: 05.20.01., Chelyabinsk (in Russian)
  • Tambiev, A.H., Gusev, M.V., Kirikova, N.N., Beckiy, O.V., Gulaev, U.V. (1986). Stimulation of growth of cyanobacteria by millimeter electromagnetic radiation of low intensiveness. Trade Exibition Microbe-86. XIX Intern. Congr. Microbiol., September 7-13:Abstr. – Manchester, England, 1986. P. 380.
  • Deviatkov, N.D., Golant, M.B., Beckiy, O.V. (1991). Millimeter waves and their role in life processes, Moscow (in Russian)
  • Karpovich, V.A., Ermolovich, A.A., Mikhalenko, E.G., Barashkova, G.P., Novikov, O.T. (2004). New stimulating and disinfecting microwave technology for presowing processing of vegetable seeds. Agropanorama. 4, 17. (in Russian)
  • Klunduk, G.A. (2004). Substantiation of electrotechnological regimes of microwave treatment of flax seeds: the dissertation of the candidate tehn. sciences: 05.20.02. Krasnoyarsk, 156. (in Russian)
  • Tambiev, A.H., Kirikova, N.N., Beckiy, O.V., Gulaev, U.V. (2003). Millimeter waves and photosynthesizing organisms. J. Radio engineering, 175. (in Russian)
  • Tambiev, A.H. (2009). Regularities manifested by the action of low-intensity waves on photosynthetic organisms. J. Millimeter waves in biology and medicine, 56(4), 56-68. (in Russian)
  • Chilochi, A.A., Tyurina, Zh. P., Klapko, S.F., Stratan, M.V., Lablyuk, S.V., Dvornina, E.G., Kondruk, V.F. (2011). Effect of millimeter-range electromagnetic radiation on the biosynthesis of extracellular hydrolytic enzymes in Aspergillus and Penicillium micromycetes J. Surface Engineering and App. Electrochem, 47(6), 558–564.
  • Debouzya, J.-C., Crouziera, D., Dabouisa, V., Malabiaub, R., Bacheleta, C., Perrina, A. (2007) Effets biologiques des rayonnements millimétriques (94 GHz). Quelles conséquences à long terme? Biologic effects of millimeteric waves (94 GHz). Are there long term consequences? J. Pathologie biologie, 55(5), 246–255.
  • Volynets, A.P. (2013). Phenolic compounds in the life of plants. Minsk (in Russian)
  • Karpovich, B.A., Rodionova, V.N. (2003). The method of presowing treatment of seeds of vegetable or grain crops. Patent of Belarus, №5580. (in Russian)
  • State Pharmacopoeia of the Republic of Belarus (2008) V.2, p.381. (in Russian)
  • Wang, M., Simon, J., Aviles, I., He, K., Zheng, Q., Tadmor, Y. (2003). Analysis of antioxidative phenolic compounds in artichoke (Cynara scolymus L.) J. of agricultural and food chemistry, 51(3), 601-608.
  • Fuksman, I.L. (1999) Influence of natural and anthropogenic factors on the metabolism of substances of secondary origin in woody plants: the dissertation author's abstract of the doctor of biol. sciences: 03.00.16, St. Petersburg. (in Russian)
  • Zaprometov, M.N. (1974) Basics of biochemistry of phenolic compounds. Moscow. (in Russian)
  • Lomboeva, S.S., Tankhaeva, L.M., Olennikov, D.N. (2008) Dynamics of accumulation of flavonoids in the aerial part of the ortilia with a one-sided (Orthilia secunda (L.) House) J. Chemistry of plant raw material, 3, 86-88. (in Russian)
  • Kopach, O.V., Pushkina, N.V., Kuzovkova, A.A., Karpovich, V.A. (2015). Exposure of ultra high frequency low power electromagnetic field on the biologically active substances biosynthesis in Silybum marianum L. cell cultures. J. Proceedings of the National Academy of Sciences of Belarus, Biological Series, 2, 5-8. (in Russian)
  • Kovzunova, O.V., Pushkina, N.V., Аzizbekian, S.G. (2017) In vitro culture Silybum marianum (L.) as a potential source of target BAS. J. Problems of biological, medical and pharmaceutical chemistry, 20(7), 3-6. (in Russian)
  • Shysh, S., Mazets, Z., Shutava, H. (2017). Efficiency of water and water-alcohol extraction of Сalendula officinalis raw by using various kinds of presowing processing. J. Problems of biological, medical and pharmaceutical chemistry, 6, 35- 40. (in Russian)

Electromagnetic radiation of low intensity as a factor of change of phenolic compounds content

Year 2018, Volume: 5 Issue: 3, 252 - 258, 15.09.2018
https://doi.org/10.21448/ijsm.459102

Abstract

The
influence of low-intensity electromagnetic radiation (EMR) on the content of
phenolic compounds (PC) in plants on different stages of ontogeny is discussed.
A medicinal plant (Calendula officinalis L.) and agricultural crop Fagopyrum
sagittatum G., diploid varieties and tetraploid varieties  were chosen as objects of study. Microwave
EMR in two frequency bands, 53.57-78.33 GHz (wide range mode) and 64.00-66.00
GHz (narrow range mode), has been selected to learn the physical effects on
seeds, with a treatment exposure time of 20, 12, or 8 min. The experiments were
conducted under laboratory and field conditions. Treated and control seeds were
germinated by 100 pcs in a growth chamber on moistened filter paper at 22–24˚C
in the dark and placed on the daylight from the third day. The level of PC in
the seedlings was measured with the Folin-Ciocalteu reagent. It was found that
in the EMR pretreated C. officinalis seedlings the level of PC is maximal on
14th day of germination. The wide mode treatment for 12 min results in a
two-fold increase in the level of PC in juvenile shoots with respect to
control. Different constitutional levels of phenolic compounds in the leaves of
diploid and tetraploid varieties of F. sagittatum in the most critical phases
of ontogeny were found. It is recommended to use EMR with a 12 min exposure
time for C. officinalis. The narrow frequency mode with an exposure time of 20 min
is preferable to use for most varieties of F. sagittatum.

References

  • Zaprometov, M.N. (1993). Physiology and biochemistry of flavonoids. J. Physiology of plants, 40(6), 921–931. (in Russian)
  • Gorgun, Y., Mazets, Z., Shysh, S., Elovskaya, N. (2017). Influence of low-intensity electromagnetic radiation on the dynamics of accumulation of phenolic compounds in buckwheat plants tetraploid. «Modern Problems of Natural Science in Science and the Educational Process» Materials of the Republican Scientific and Practical Conference. Minsk (in Russian).
  • Jambazishvili, S.N. (1974). Presowing treatment of seeds of sugar beet by various electrophysical methods and their influence on productivity and quality of root crops: the dissertation author's abstract the candidate of agricultural sciences: 06.01.09, Tbilisi (in Russian)
  • Novikova, G.V. (1980). Substantiation and research of technological process of influence of EMF microwave on seeds of vegetable cultures: the dissertation of the candidate tehn. sciences: 05.20.01., Chelyabinsk (in Russian)
  • Tambiev, A.H., Gusev, M.V., Kirikova, N.N., Beckiy, O.V., Gulaev, U.V. (1986). Stimulation of growth of cyanobacteria by millimeter electromagnetic radiation of low intensiveness. Trade Exibition Microbe-86. XIX Intern. Congr. Microbiol., September 7-13:Abstr. – Manchester, England, 1986. P. 380.
  • Deviatkov, N.D., Golant, M.B., Beckiy, O.V. (1991). Millimeter waves and their role in life processes, Moscow (in Russian)
  • Karpovich, V.A., Ermolovich, A.A., Mikhalenko, E.G., Barashkova, G.P., Novikov, O.T. (2004). New stimulating and disinfecting microwave technology for presowing processing of vegetable seeds. Agropanorama. 4, 17. (in Russian)
  • Klunduk, G.A. (2004). Substantiation of electrotechnological regimes of microwave treatment of flax seeds: the dissertation of the candidate tehn. sciences: 05.20.02. Krasnoyarsk, 156. (in Russian)
  • Tambiev, A.H., Kirikova, N.N., Beckiy, O.V., Gulaev, U.V. (2003). Millimeter waves and photosynthesizing organisms. J. Radio engineering, 175. (in Russian)
  • Tambiev, A.H. (2009). Regularities manifested by the action of low-intensity waves on photosynthetic organisms. J. Millimeter waves in biology and medicine, 56(4), 56-68. (in Russian)
  • Chilochi, A.A., Tyurina, Zh. P., Klapko, S.F., Stratan, M.V., Lablyuk, S.V., Dvornina, E.G., Kondruk, V.F. (2011). Effect of millimeter-range electromagnetic radiation on the biosynthesis of extracellular hydrolytic enzymes in Aspergillus and Penicillium micromycetes J. Surface Engineering and App. Electrochem, 47(6), 558–564.
  • Debouzya, J.-C., Crouziera, D., Dabouisa, V., Malabiaub, R., Bacheleta, C., Perrina, A. (2007) Effets biologiques des rayonnements millimétriques (94 GHz). Quelles conséquences à long terme? Biologic effects of millimeteric waves (94 GHz). Are there long term consequences? J. Pathologie biologie, 55(5), 246–255.
  • Volynets, A.P. (2013). Phenolic compounds in the life of plants. Minsk (in Russian)
  • Karpovich, B.A., Rodionova, V.N. (2003). The method of presowing treatment of seeds of vegetable or grain crops. Patent of Belarus, №5580. (in Russian)
  • State Pharmacopoeia of the Republic of Belarus (2008) V.2, p.381. (in Russian)
  • Wang, M., Simon, J., Aviles, I., He, K., Zheng, Q., Tadmor, Y. (2003). Analysis of antioxidative phenolic compounds in artichoke (Cynara scolymus L.) J. of agricultural and food chemistry, 51(3), 601-608.
  • Fuksman, I.L. (1999) Influence of natural and anthropogenic factors on the metabolism of substances of secondary origin in woody plants: the dissertation author's abstract of the doctor of biol. sciences: 03.00.16, St. Petersburg. (in Russian)
  • Zaprometov, M.N. (1974) Basics of biochemistry of phenolic compounds. Moscow. (in Russian)
  • Lomboeva, S.S., Tankhaeva, L.M., Olennikov, D.N. (2008) Dynamics of accumulation of flavonoids in the aerial part of the ortilia with a one-sided (Orthilia secunda (L.) House) J. Chemistry of plant raw material, 3, 86-88. (in Russian)
  • Kopach, O.V., Pushkina, N.V., Kuzovkova, A.A., Karpovich, V.A. (2015). Exposure of ultra high frequency low power electromagnetic field on the biologically active substances biosynthesis in Silybum marianum L. cell cultures. J. Proceedings of the National Academy of Sciences of Belarus, Biological Series, 2, 5-8. (in Russian)
  • Kovzunova, O.V., Pushkina, N.V., Аzizbekian, S.G. (2017) In vitro culture Silybum marianum (L.) as a potential source of target BAS. J. Problems of biological, medical and pharmaceutical chemistry, 20(7), 3-6. (in Russian)
  • Shysh, S., Mazets, Z., Shutava, H. (2017). Efficiency of water and water-alcohol extraction of Сalendula officinalis raw by using various kinds of presowing processing. J. Problems of biological, medical and pharmaceutical chemistry, 6, 35- 40. (in Russian)
There are 22 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Svetlana Shysh This is me

Zhanna Mazets This is me

Hanna Shutava This is me

Olga Sysha This is me

Yulia Gorgun This is me

Publication Date September 15, 2018
Submission Date March 28, 2018
Published in Issue Year 2018 Volume: 5 Issue: 3

Cite

APA Shysh, S., Mazets, Z., Shutava, H., Sysha, O., et al. (2018). Electromagnetic radiation of low intensity as a factor of change of phenolic compounds content. International Journal of Secondary Metabolite, 5(3), 252-258. https://doi.org/10.21448/ijsm.459102
International Journal of Secondary Metabolite

e-ISSN: 2148-6905