Agrobacterium – caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L.

Year 2018, Volume: 5 Issue: 4, 312 - 318, 29.12.2018

Olha Mikolaivna Yaroshko Mykola Vyktorovych Kuchuk

https://doi.org/10.21448/ijsm.478267

Cited By: 10

Abstract

The procedure for vacuum infiltration of cultivars A. caudatus L. and hybrids of A. caudatus L. xA. paniculatus L. was optimized. The functioning of gene construction pCBv19 in the Amaranthus leaves was evaluated by the transient expression after vacuum infiltration with Agrobacterium rhizogenes A4. After hypocotyl transformation of the varieties of amaranth species A. caudatus L.: Helios, Karmin, Kremovyi rannii, and hybrids A. caudatus x A. paniculatus L. – cv. Sterkh, A. caudatus x Sterkh- cv. Zhaivir with the wild strain A. rhizogenes A4, the culture of "hairy roots" was obtained. Embedding and transcription of genes in the roots are confirmed by the results of the PCR analysis.

Keywords

Amaranthus, Agrobacterium, transformation, transgenic roots.

Agrobacterium – caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L.

Abstract

The procedure for vacuum infiltration of cultivars
A. caudatus L. and hybrids of A. caudatus
L. _xA. paniculatus L. was
optimized. The functioning of
gene construction pCBv19 in the Amaranthus
leaves was evaluated by the transient expression after vacuum infiltration with
Agrobacterium rhizogenes A4. After hypocotyl transformation of the varieties
of amaranth species  A. caudatus L.: Helios, Karmin, Kremovyi rannii, and hybrids A. caudatus x A. paniculatus L. – cv. Sterkh,
A. caudatus x Sterkh- cv. Zhaivir with the wild strain A. rhizogenes A4, the culture of "hairy roots" was obtained. Embedding and transcription of genes in the
roots are confirmed by the results of the PCR analysis.

Keywords

Amaranthus, Agrobacterium, transformation, transgenic roots

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