Antioxidant and anti-inflammatory activity of capitula, leaf and stem extracts of Tanacetum cilicicum (Boiss.) Grierson

Year 2019, Volume: 6 Issue: 2, 211 - 222, 15.07.2019

Aybeniz Yıldırım , Ali Şen , Ahmet Doğan , Leyla Bitis

https://doi.org/10.21448/ijsm.510316

Cited By: 17

Abstract

In this study, various extracts obtained different parts of Tanacetum cilicicum were investigated for in vitro antioxidant and anti-inflammatory activity. Antioxidant activity was tested with three methods; namely 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, 2.2′‐azinobis (3‐ethylbenzothiazolin‐6‐sulphonic acid) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP) assays. Total phenolic and flavonoid contents of extracts were determined by Folin-Ciocalteu and aluminum chloride methods, respectively. Also, anti-inflammatory activity of these extracts was evaluated by 5-lipoxygenase inhibition assay. Ethyl acetate extract of capitula of T. cilicicum (TCCEA) showed the highest antioxidant activity with IC50 values of 22.44 and 30.86 µg/mL against DPPH and ABTS radicals, respectively. At the same time, the highest ferric reducing power was found in the TCCEA (42.2 mg TE/g extract). The highest total phenolic contents have been detected in TCCEA and ethyl acetate extract of leaves of T. cilicicum (TCLEA) with value of 174.1 and 175.6 mg GAE/g extract, respectively. Similarly, the highest total flavonoid contents have been detected in TCCEA and TCLEA with values of 26.94 and 30.48 mg QE/g extract, respectively. TCCEA exhibited strong anti-inflammatory activity with IC50 value of 9.44 µg/mL when compared to standard indomethacine (22.39 µg/mL). These results demonstrate that TCCEA has a significant antioxidant and anti-inflammatory activity. Also, the results show that TCCEA is a good candidate for further bioactivity-guided fractionation in the search for new active anti-inflammatory and antioxidant compounds.

Antioxidant and anti-inflammatory activity of capitula, leaf and stem extracts of Tanacetum cilicicum (Boiss.) Grierson

Abstract

In
this study, various extracts obtained different parts of Tanacetum cilicicum were investigated for in vitro antioxidant and anti-inflammatory activity. Antioxidant
activity was tested with three methods; namely 2.2-diphenyl-1-picrylhydrazyl
(DPPH) radical scavenging capacity, 2.2′‐azinobis
(3‐ethylbenzothiazolin‐6‐sulphonic acid) (ABTS) radical cation scavenging
capacity, and ferric-reducing antioxidant power (FRAP) assays. Total phenolic
and flavonoid contents of extracts were determined by Folin-Ciocalteu and
aluminum chloride methods, respectively. Also, anti-inflammatory activity of
these extracts was evaluated by 5-lipoxygenase inhibition assay. Ethyl acetate
extract of capitula of T. cilicicum
(TCCEA) showed the highest antioxidant activity with IC₅₀ values of
22.44 and 30.86 µg/mL against DPPH and ABTS radicals, respectively. At the same
time, the highest ferric reducing power was found in the TCCEA (42.2 mg TE/g
extract). The highest total phenolic contents have been detected in TCCEA and
ethyl acetate extract of leaves of T.
cilicicum (TCLEA) with value of 174.1 and 175.6 mg GAE/g extract,
respectively. Similarly, the highest total flavonoid contents have been
detected in TCCEA and TCLEA with values of 26.94 and 30.48 mg QE/g extract,
respectively. TCCEA exhibited strong anti-inflammatory activity with IC₅₀
value of 9.44 µg/mL when compared to standard indomethacine (22.39 µg/mL).
These results demonstrate that TCCEA has a significant antioxidant and
anti-inflammatory activity. Also, the results show that TCCEA is a good
candidate for further bioactivity-guided fractionation in the search for new
active anti-inflammatory and antioxidant compounds.

Keywords

Tanacetum cilicicum, Anti-inflammatory activity, Antioxidant activity, Total phenol content

References

• [1]. Sosa, S., Balick, M.J., Arvigo, R., Esposito, R.G., Pizza, C., Altinier, G., Tubaro, A. (2002). Screening of the topical anti-inflammatory activity of some Central American plants. J Ethnopharmacol., 81(2), 211-215.
• [2]. Tunon, H., Olavsdotter, C., Bohlin, L. (1995). Evaluation of anti-inflammatory activity of some Swedish medicinal plants. Inhibition of prostaglandin biosynthesis and PAF-induced exocytosis. J Ethnopharmacol., 48(2), 61-76.
• [3]. Li, R.W., Myers, S.P., Leach, D.N., Lin, G.D., Leach, G. (2003). A cross-cultural study: anti-inflammatory activity of Australian and Chinese plants. J Ethnopharmacol., 85(1), 25-32.
• [4]. Sheeja, K., Shihab, P.K., Kuttan G. (2006). Antioxidant and Anti-Inflammatory Activities of the Plant Andrographis Paniculata Nees. Immunopharmacol Immunotoxicol, 28. 129–140.
• [5]. Schinella, G.R., Tournier, H.A., Prieto, J.M., Mordujovich de Buschiazzo, P., Ríos, J.L. (2002). Antioxidant activity of anti-inflammatory plant extracts. Life Sci., 70, 1023–1033.
• [6]. Aruoma, O.I. (1998). Free radicals, oxidative stress, and antioxidants in human health and disease. J Am Oil Chem Soc., 75(2), 199–212.
• [7]. Pham-Huy, L.A., He, H., Pham-Huy, C. (2008). Free Radicals, Antioxidants in Disease and Health. Int J Biomed Sci., 4(2), 89–96.
• [8]. Diaz, P., Jeong, S.C., Lee, S., Khoo, C., Koyyalamudi, S.R. (2012). Antioxidant and anti-inflammatory activities of selected medicinal plants and fungi containing phenolic and flavonoid compounds. Chin Med., 7(1), 1-13.
• [9]. Arıtuluk, Z.C., Çankaya, İ.İ.T., Özkan, A.M.G. (2016). Antioxidant Activity, Total Phenolic and Flavonoid Contents of Some Tanacetum L. (Asteraceae) Taxa Growing in Turkey. FABAD J Pharm Sci., 41, 17-25.
• [10]. Özbilgin, S., Akkol, E.K., Öz, B.E., Ilhan, M., Saltan, G., Acıkara, Ö.B., Tekin, M., Keleş, H., Süntar, İ. (2018). In vivo activity assessment of some Tanacetum species used as traditional wound healer along with identification of the phytochemical profile by a new validated HPLC method. Iran J Basic Med Sci., 21(2), 145–152.
• [11]. Davis, P.H. (1975). Flora of Turkey, Volume 5.; Edinburgh University Press: Edinburgh, 1975; pp. 261; ISBN: 0 85224 280 8.
• [12]. Gecibesler, İ.H. (2017). In Vıtro Bıologıcal Actıvıty Studıes on Tanacetum abrotanıfolıum (L.) Druce (Asteraceae). Anadolu Univ J of Sci and Technology A – Appl Sci and Eng, 18(2), 439- 455.
• [13]. Williams, C.A., Harborne, J.B., Geiger, H., Hoult, J.R. (1999). The flavonoids of Tanacetum parthenium and T. vulgare and their anti-inflammatory properties. Phytochemistry, 51, 417–423.
• [14]. Albayrak, G., Nalbantsoy, A., Baykan, Ş. (2017). In Vitro Cytotoxic and Anti-inflammatory Activities of Tanacetum argenteum (Lam.) Willd. subsp. argenteum Extract. Turk J Pharm Sci., 14(3), 231-236.
• [15]. Abad, M.J., Bermejo, P., Villar, A. (1995). An approach to the genus Tanacetum L. (Compositae): Phytochemical and pharmacological review. Phytother Res., 9(2), 79-92.
• [16]. Gören, N., Arda, N., Çaliskan, Z. (2002). Chemical characterization and biological activities of the genus Tanacetum (Compositae). Stud in Nat Prod Chem., 27, 547-658.
• [17]. Sen, A., Ozakpinar, Ö.B, Tan, S.B., Kültür, S., Uras, F., Bitis, L. (2017). Biological activities of aerial parts extracts of endemic Tanacetum argenteum subsp. Argenteum. Marmara Pharm J, 21(2), 286-290.
• [18]. Gecibesler, İ.H., Kocak, A., Demirtas, I. (2016). Biological activities, phenolic profiles and essential oil components of Tanacetum cilicicum (BOISS.) GRIERSON. Nat Prod Res., 30(24), 2850-2855.
• [19]. Goren, N., Arda, N., Caliskan, Z. (2002). Chemical characterization and biological activities of the genus Tanacetum (Compositae). Stud Nat Prod Chem., 27, 547-658.
• [20]. Polatoglu, K., Demirci, F., Demirci, B., Goren, N., Baser, K.H.C. (2010). Essential oil composition and antibacterial activity of Tanacetum argenteum (Lam.) Willd. ssp. argenteum and T. densum (Lab.) Schultz Bip. ssp. amani heywood from Turkey. J Oleo Sci., 59, 361-7.
• [21]. Ulukanli, Z., Demirci, S., Yilmaztekin, M. (2017). Essential Oil Constituents of Tanacetum cilicicum: Antimicrobial and Phytotoxic Activities. J Food Qual., 2017, 1-11.
• [22]. Zou, Y., Chang, S.K., Gu, Y., Qian, S.Y. (2011). Antioxidant activity and phenolic compositions of lentil (Lens culinaris var. morton) extract and its fractions. J Agric Food Chem., 59(6), 2268-2276.
• [23]. Gao, X., Ohlander, M., Jeppsson, N., Björk, L., Trajkovski, V. (2000). Changes in antioxidant effects and their relationship to phytonutrients in fruits of Sea Buckthorn (Hippophae rhamnoides L.) during maturation. J Agric Food Chem., 48, 1485-1490.
• [24]. Zhang, R., Zeng, Q., Deng, Y., Zhang, M., Wei, Z., Zhang, Y., Tang, X. (2013). Phenolic profiles and antioxidant activity of litchi pulp of different cultivars cultivated in Southern China. Food Chem., 136, 1169-1176.
• [25]. Phosrithong, N., Nuchtavorn, N. (2016). Antioxidant and anti-inflammatory activites of Clerodendrum leaf extracts collected in Thailand. Eur J Integr Med., 8, 281-285.
• [26]. See, I., Ee, G.C.L., Mah, S.H., Jong, V.Y.M., The, S.S. (2017). Effect of Solvents on Phytochemical Concentrations and Antioxidant Activity of Garcinia benthamiana Stem Bark Extracts. J Herbs Spices Med Plants, 23, 117-127.
• [27]. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 26(9-10), 1231-1237.
• [28]. Çalişkan, Z., Gören, N. (2018). Sesquiterpene Lactones and Other Constituents from The Aerial Parts of Tanacetum abrotonifolium Druce, Collected in East Turkey. J Turkish Chem Soc Sec Chem., 5, 511-520.
• [29]. Martins, A., Mignon, R., Bastos, M., Batista, D., Neng, NR., Nogueira, J.M., Vizetto-Duarte, C., Custódio, L., Varela, J., Rauter, A.P. 2014. In vitro antitumoral activity of compounds isolated from Artemisia gorgonum Webb. Phytother Res., 28(9), 1329-1334.
• [30]. Tournier, H., Schinella, G., de Balsa, E.M., Buschiazzo, H., Mañez, S., Mordujovich de Buschiazzo, P. (1999). Effect of the chloroform extract of Tanacetum vulgare and one of its active principles, parthenolide, on experimental gastric ulcer in rats. J Pharm Pharmacol. 51(2), 215-219.
• [31]. Ghasemi, P.A., Momeni, M., Bahmani, M. (2013). Ethnobotanical study of medicinal plants used by Kurd tribe in Dehloran and Abdanan districts, Ilam province, Iran. Afr J Tradit Complement Altern Med., 10(2), 368-385.
• [32]. Serafini, M., Peluso, I., Raguzzini, A. (2010). Flavonoids as anti-inflammatory agents. Proc Nutr Soc., 69(3), 273-278.