Research Article
BibTex RIS Cite

Antioxidant and anti-inflammatory activity of capitula, leaf and stem extracts of Tanacetum cilicicum (Boiss.) Grierson

Year 2019, Volume: 6 Issue: 2, 211 - 222, 15.07.2019
https://doi.org/10.21448/ijsm.510316

Abstract

In this study, various extracts obtained different parts of Tanacetum cilicicum were investigated for in vitro antioxidant and anti-inflammatory activity. Antioxidant activity was tested with three methods; namely 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, 2.2′‐azinobis (3‐ethylbenzothiazolin‐6‐sulphonic acid) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP) assays. Total phenolic and flavonoid contents of extracts were determined by Folin-Ciocalteu and aluminum chloride methods, respectively. Also, anti-inflammatory activity of these extracts was evaluated by 5-lipoxygenase inhibition assay. Ethyl acetate extract of capitula of T. cilicicum (TCCEA) showed the highest antioxidant activity with IC50 values of 22.44 and 30.86 µg/mL against DPPH and ABTS radicals, respectively. At the same time, the highest ferric reducing power was found in the TCCEA (42.2 mg TE/g extract). The highest total phenolic contents have been detected in TCCEA and ethyl acetate extract of leaves of T. cilicicum (TCLEA) with value of 174.1 and 175.6 mg GAE/g extract, respectively. Similarly, the highest total flavonoid contents have been detected in TCCEA and TCLEA with values of 26.94 and 30.48 mg QE/g extract, respectively. TCCEA exhibited strong anti-inflammatory activity with IC50 value of 9.44 µg/mL when compared to standard indomethacine (22.39 µg/mL). These results demonstrate that TCCEA has a significant antioxidant and anti-inflammatory activity. Also, the results show that TCCEA is a good candidate for further bioactivity-guided fractionation in the search for new active anti-inflammatory and antioxidant compounds.

References

  • [1]. Sosa, S., Balick, M.J., Arvigo, R., Esposito, R.G., Pizza, C., Altinier, G., Tubaro, A. (2002). Screening of the topical anti-inflammatory activity of some Central American plants. J Ethnopharmacol., 81(2), 211-215.
  • [2]. Tunon, H., Olavsdotter, C., Bohlin, L. (1995). Evaluation of anti-inflammatory activity of some Swedish medicinal plants. Inhibition of prostaglandin biosynthesis and PAF-induced exocytosis. J Ethnopharmacol., 48(2), 61-76.
  • [3]. Li, R.W., Myers, S.P., Leach, D.N., Lin, G.D., Leach, G. (2003). A cross-cultural study: anti-inflammatory activity of Australian and Chinese plants. J Ethnopharmacol., 85(1), 25-32.
  • [4]. Sheeja, K., Shihab, P.K., Kuttan G. (2006). Antioxidant and Anti-Inflammatory Activities of the Plant Andrographis Paniculata Nees. Immunopharmacol Immunotoxicol, 28. 129–140.
  • [5]. Schinella, G.R., Tournier, H.A., Prieto, J.M., Mordujovich de Buschiazzo, P., Ríos, J.L. (2002). Antioxidant activity of anti-inflammatory plant extracts. Life Sci., 70, 1023–1033.
  • [6]. Aruoma, O.I. (1998). Free radicals, oxidative stress, and antioxidants in human health and disease. J Am Oil Chem Soc., 75(2), 199–212.
  • [7]. Pham-Huy, L.A., He, H., Pham-Huy, C. (2008). Free Radicals, Antioxidants in Disease and Health. Int J Biomed Sci., 4(2), 89–96.
  • [8]. Diaz, P., Jeong, S.C., Lee, S., Khoo, C., Koyyalamudi, S.R. (2012). Antioxidant and anti-inflammatory activities of selected medicinal plants and fungi containing phenolic and flavonoid compounds. Chin Med., 7(1), 1-13.
  • [9]. Arıtuluk, Z.C., Çankaya, İ.İ.T., Özkan, A.M.G. (2016). Antioxidant Activity, Total Phenolic and Flavonoid Contents of Some Tanacetum L. (Asteraceae) Taxa Growing in Turkey. FABAD J Pharm Sci., 41, 17-25.
  • [10]. Özbilgin, S., Akkol, E.K., Öz, B.E., Ilhan, M., Saltan, G., Acıkara, Ö.B., Tekin, M., Keleş, H., Süntar, İ. (2018). In vivo activity assessment of some Tanacetum species used as traditional wound healer along with identification of the phytochemical profile by a new validated HPLC method. Iran J Basic Med Sci., 21(2), 145–152.
  • [11]. Davis, P.H. (1975). Flora of Turkey, Volume 5.; Edinburgh University Press: Edinburgh, 1975; pp. 261; ISBN: 0 85224 280 8.
  • [12]. Gecibesler, İ.H. (2017). In Vıtro Bıologıcal Actıvıty Studıes on Tanacetum abrotanıfolıum (L.) Druce (Asteraceae). Anadolu Univ J of Sci and Technology A – Appl Sci and Eng, 18(2), 439- 455.
  • [13]. Williams, C.A., Harborne, J.B., Geiger, H., Hoult, J.R. (1999). The flavonoids of Tanacetum parthenium and T. vulgare and their anti-inflammatory properties. Phytochemistry, 51, 417–423.
  • [14]. Albayrak, G., Nalbantsoy, A., Baykan, Ş. (2017). In Vitro Cytotoxic and Anti-inflammatory Activities of Tanacetum argenteum (Lam.) Willd. subsp. argenteum Extract. Turk J Pharm Sci., 14(3), 231-236.
  • [15]. Abad, M.J., Bermejo, P., Villar, A. (1995). An approach to the genus Tanacetum L. (Compositae): Phytochemical and pharmacological review. Phytother Res., 9(2), 79-92.
  • [16]. Gören, N., Arda, N., Çaliskan, Z. (2002). Chemical characterization and biological activities of the genus Tanacetum (Compositae). Stud in Nat Prod Chem., 27, 547-658.
  • [17]. Sen, A., Ozakpinar, Ö.B, Tan, S.B., Kültür, S., Uras, F., Bitis, L. (2017). Biological activities of aerial parts extracts of endemic Tanacetum argenteum subsp. Argenteum. Marmara Pharm J, 21(2), 286-290.
  • [18]. Gecibesler, İ.H., Kocak, A., Demirtas, I. (2016). Biological activities, phenolic profiles and essential oil components of Tanacetum cilicicum (BOISS.) GRIERSON. Nat Prod Res., 30(24), 2850-2855.
  • [19]. Goren, N., Arda, N., Caliskan, Z. (2002). Chemical characterization and biological activities of the genus Tanacetum (Compositae). Stud Nat Prod Chem., 27, 547-658.
  • [20]. Polatoglu, K., Demirci, F., Demirci, B., Goren, N., Baser, K.H.C. (2010). Essential oil composition and antibacterial activity of Tanacetum argenteum (Lam.) Willd. ssp. argenteum and T. densum (Lab.) Schultz Bip. ssp. amani heywood from Turkey. J Oleo Sci., 59, 361-7.
  • [21]. Ulukanli, Z., Demirci, S., Yilmaztekin, M. (2017). Essential Oil Constituents of Tanacetum cilicicum: Antimicrobial and Phytotoxic Activities. J Food Qual., 2017, 1-11.
  • [22]. Zou, Y., Chang, S.K., Gu, Y., Qian, S.Y. (2011). Antioxidant activity and phenolic compositions of lentil (Lens culinaris var. morton) extract and its fractions. J Agric Food Chem., 59(6), 2268-2276.
  • [23]. Gao, X., Ohlander, M., Jeppsson, N., Björk, L., Trajkovski, V. (2000). Changes in antioxidant effects and their relationship to phytonutrients in fruits of Sea Buckthorn (Hippophae rhamnoides L.) during maturation. J Agric Food Chem., 48, 1485-1490.
  • [24]. Zhang, R., Zeng, Q., Deng, Y., Zhang, M., Wei, Z., Zhang, Y., Tang, X. (2013). Phenolic profiles and antioxidant activity of litchi pulp of different cultivars cultivated in Southern China. Food Chem., 136, 1169-1176.
  • [25]. Phosrithong, N., Nuchtavorn, N. (2016). Antioxidant and anti-inflammatory activites of Clerodendrum leaf extracts collected in Thailand. Eur J Integr Med., 8, 281-285.
  • [26]. See, I., Ee, G.C.L., Mah, S.H., Jong, V.Y.M., The, S.S. (2017). Effect of Solvents on Phytochemical Concentrations and Antioxidant Activity of Garcinia benthamiana Stem Bark Extracts. J Herbs Spices Med Plants, 23, 117-127.
  • [27]. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 26(9-10), 1231-1237.
  • [28]. Çalişkan, Z., Gören, N. (2018). Sesquiterpene Lactones and Other Constituents from The Aerial Parts of Tanacetum abrotonifolium Druce, Collected in East Turkey. J Turkish Chem Soc Sec Chem., 5, 511-520.
  • [29]. Martins, A., Mignon, R., Bastos, M., Batista, D., Neng, NR., Nogueira, J.M., Vizetto-Duarte, C., Custódio, L., Varela, J., Rauter, A.P. 2014. In vitro antitumoral activity of compounds isolated from Artemisia gorgonum Webb. Phytother Res., 28(9), 1329-1334.
  • [30]. Tournier, H., Schinella, G., de Balsa, E.M., Buschiazzo, H., Mañez, S., Mordujovich de Buschiazzo, P. (1999). Effect of the chloroform extract of Tanacetum vulgare and one of its active principles, parthenolide, on experimental gastric ulcer in rats. J Pharm Pharmacol. 51(2), 215-219.
  • [31]. Ghasemi, P.A., Momeni, M., Bahmani, M. (2013). Ethnobotanical study of medicinal plants used by Kurd tribe in Dehloran and Abdanan districts, Ilam province, Iran. Afr J Tradit Complement Altern Med., 10(2), 368-385.
  • [32]. Serafini, M., Peluso, I., Raguzzini, A. (2010). Flavonoids as anti-inflammatory agents. Proc Nutr Soc., 69(3), 273-278.

Antioxidant and anti-inflammatory activity of capitula, leaf and stem extracts of Tanacetum cilicicum (Boiss.) Grierson

Year 2019, Volume: 6 Issue: 2, 211 - 222, 15.07.2019
https://doi.org/10.21448/ijsm.510316

Abstract

In
this study, various extracts obtained different parts of Tanacetum cilicicum were investigated for in vitro antioxidant and anti-inflammatory activity. Antioxidant
activity was tested with three methods; namely 2.2-diphenyl-1-picrylhydrazyl
(DPPH) radical scavenging capacity, 2.2′‐azinobis
(3‐ethylbenzothiazolin‐6‐sulphonic acid) (ABTS) radical cation scavenging
capacity, and ferric-reducing antioxidant power (FRAP) assays. Total phenolic
and flavonoid contents of extracts were determined by Folin-Ciocalteu and
aluminum chloride methods, respectively. Also, anti-inflammatory activity of
these extracts was evaluated by 5-lipoxygenase inhibition assay. Ethyl acetate
extract of capitula of T. cilicicum
(TCCEA) showed the highest antioxidant activity with IC50 values of
22.44 and 30.86 µg/mL against DPPH and ABTS radicals, respectively. At the same
time, the highest ferric reducing power was found in the TCCEA (42.2 mg TE/g
extract). The highest total phenolic contents have been detected in TCCEA and
ethyl acetate extract of leaves of T.
cilicicum
(TCLEA) with value of 174.1 and 175.6 mg GAE/g extract,
respectively. Similarly, the highest total flavonoid contents have been
detected in TCCEA and TCLEA with values of 26.94 and 30.48 mg QE/g extract,
respectively. TCCEA exhibited strong anti-inflammatory activity with IC50
value of 9.44 µg/mL when compared to standard indomethacine (22.39 µg/mL).
These results demonstrate that TCCEA has a significant antioxidant and
anti-inflammatory activity. Also, the results show that TCCEA is a good
candidate for further bioactivity-guided fractionation in the search for new
active anti-inflammatory and antioxidant compounds.

References

  • [1]. Sosa, S., Balick, M.J., Arvigo, R., Esposito, R.G., Pizza, C., Altinier, G., Tubaro, A. (2002). Screening of the topical anti-inflammatory activity of some Central American plants. J Ethnopharmacol., 81(2), 211-215.
  • [2]. Tunon, H., Olavsdotter, C., Bohlin, L. (1995). Evaluation of anti-inflammatory activity of some Swedish medicinal plants. Inhibition of prostaglandin biosynthesis and PAF-induced exocytosis. J Ethnopharmacol., 48(2), 61-76.
  • [3]. Li, R.W., Myers, S.P., Leach, D.N., Lin, G.D., Leach, G. (2003). A cross-cultural study: anti-inflammatory activity of Australian and Chinese plants. J Ethnopharmacol., 85(1), 25-32.
  • [4]. Sheeja, K., Shihab, P.K., Kuttan G. (2006). Antioxidant and Anti-Inflammatory Activities of the Plant Andrographis Paniculata Nees. Immunopharmacol Immunotoxicol, 28. 129–140.
  • [5]. Schinella, G.R., Tournier, H.A., Prieto, J.M., Mordujovich de Buschiazzo, P., Ríos, J.L. (2002). Antioxidant activity of anti-inflammatory plant extracts. Life Sci., 70, 1023–1033.
  • [6]. Aruoma, O.I. (1998). Free radicals, oxidative stress, and antioxidants in human health and disease. J Am Oil Chem Soc., 75(2), 199–212.
  • [7]. Pham-Huy, L.A., He, H., Pham-Huy, C. (2008). Free Radicals, Antioxidants in Disease and Health. Int J Biomed Sci., 4(2), 89–96.
  • [8]. Diaz, P., Jeong, S.C., Lee, S., Khoo, C., Koyyalamudi, S.R. (2012). Antioxidant and anti-inflammatory activities of selected medicinal plants and fungi containing phenolic and flavonoid compounds. Chin Med., 7(1), 1-13.
  • [9]. Arıtuluk, Z.C., Çankaya, İ.İ.T., Özkan, A.M.G. (2016). Antioxidant Activity, Total Phenolic and Flavonoid Contents of Some Tanacetum L. (Asteraceae) Taxa Growing in Turkey. FABAD J Pharm Sci., 41, 17-25.
  • [10]. Özbilgin, S., Akkol, E.K., Öz, B.E., Ilhan, M., Saltan, G., Acıkara, Ö.B., Tekin, M., Keleş, H., Süntar, İ. (2018). In vivo activity assessment of some Tanacetum species used as traditional wound healer along with identification of the phytochemical profile by a new validated HPLC method. Iran J Basic Med Sci., 21(2), 145–152.
  • [11]. Davis, P.H. (1975). Flora of Turkey, Volume 5.; Edinburgh University Press: Edinburgh, 1975; pp. 261; ISBN: 0 85224 280 8.
  • [12]. Gecibesler, İ.H. (2017). In Vıtro Bıologıcal Actıvıty Studıes on Tanacetum abrotanıfolıum (L.) Druce (Asteraceae). Anadolu Univ J of Sci and Technology A – Appl Sci and Eng, 18(2), 439- 455.
  • [13]. Williams, C.A., Harborne, J.B., Geiger, H., Hoult, J.R. (1999). The flavonoids of Tanacetum parthenium and T. vulgare and their anti-inflammatory properties. Phytochemistry, 51, 417–423.
  • [14]. Albayrak, G., Nalbantsoy, A., Baykan, Ş. (2017). In Vitro Cytotoxic and Anti-inflammatory Activities of Tanacetum argenteum (Lam.) Willd. subsp. argenteum Extract. Turk J Pharm Sci., 14(3), 231-236.
  • [15]. Abad, M.J., Bermejo, P., Villar, A. (1995). An approach to the genus Tanacetum L. (Compositae): Phytochemical and pharmacological review. Phytother Res., 9(2), 79-92.
  • [16]. Gören, N., Arda, N., Çaliskan, Z. (2002). Chemical characterization and biological activities of the genus Tanacetum (Compositae). Stud in Nat Prod Chem., 27, 547-658.
  • [17]. Sen, A., Ozakpinar, Ö.B, Tan, S.B., Kültür, S., Uras, F., Bitis, L. (2017). Biological activities of aerial parts extracts of endemic Tanacetum argenteum subsp. Argenteum. Marmara Pharm J, 21(2), 286-290.
  • [18]. Gecibesler, İ.H., Kocak, A., Demirtas, I. (2016). Biological activities, phenolic profiles and essential oil components of Tanacetum cilicicum (BOISS.) GRIERSON. Nat Prod Res., 30(24), 2850-2855.
  • [19]. Goren, N., Arda, N., Caliskan, Z. (2002). Chemical characterization and biological activities of the genus Tanacetum (Compositae). Stud Nat Prod Chem., 27, 547-658.
  • [20]. Polatoglu, K., Demirci, F., Demirci, B., Goren, N., Baser, K.H.C. (2010). Essential oil composition and antibacterial activity of Tanacetum argenteum (Lam.) Willd. ssp. argenteum and T. densum (Lab.) Schultz Bip. ssp. amani heywood from Turkey. J Oleo Sci., 59, 361-7.
  • [21]. Ulukanli, Z., Demirci, S., Yilmaztekin, M. (2017). Essential Oil Constituents of Tanacetum cilicicum: Antimicrobial and Phytotoxic Activities. J Food Qual., 2017, 1-11.
  • [22]. Zou, Y., Chang, S.K., Gu, Y., Qian, S.Y. (2011). Antioxidant activity and phenolic compositions of lentil (Lens culinaris var. morton) extract and its fractions. J Agric Food Chem., 59(6), 2268-2276.
  • [23]. Gao, X., Ohlander, M., Jeppsson, N., Björk, L., Trajkovski, V. (2000). Changes in antioxidant effects and their relationship to phytonutrients in fruits of Sea Buckthorn (Hippophae rhamnoides L.) during maturation. J Agric Food Chem., 48, 1485-1490.
  • [24]. Zhang, R., Zeng, Q., Deng, Y., Zhang, M., Wei, Z., Zhang, Y., Tang, X. (2013). Phenolic profiles and antioxidant activity of litchi pulp of different cultivars cultivated in Southern China. Food Chem., 136, 1169-1176.
  • [25]. Phosrithong, N., Nuchtavorn, N. (2016). Antioxidant and anti-inflammatory activites of Clerodendrum leaf extracts collected in Thailand. Eur J Integr Med., 8, 281-285.
  • [26]. See, I., Ee, G.C.L., Mah, S.H., Jong, V.Y.M., The, S.S. (2017). Effect of Solvents on Phytochemical Concentrations and Antioxidant Activity of Garcinia benthamiana Stem Bark Extracts. J Herbs Spices Med Plants, 23, 117-127.
  • [27]. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 26(9-10), 1231-1237.
  • [28]. Çalişkan, Z., Gören, N. (2018). Sesquiterpene Lactones and Other Constituents from The Aerial Parts of Tanacetum abrotonifolium Druce, Collected in East Turkey. J Turkish Chem Soc Sec Chem., 5, 511-520.
  • [29]. Martins, A., Mignon, R., Bastos, M., Batista, D., Neng, NR., Nogueira, J.M., Vizetto-Duarte, C., Custódio, L., Varela, J., Rauter, A.P. 2014. In vitro antitumoral activity of compounds isolated from Artemisia gorgonum Webb. Phytother Res., 28(9), 1329-1334.
  • [30]. Tournier, H., Schinella, G., de Balsa, E.M., Buschiazzo, H., Mañez, S., Mordujovich de Buschiazzo, P. (1999). Effect of the chloroform extract of Tanacetum vulgare and one of its active principles, parthenolide, on experimental gastric ulcer in rats. J Pharm Pharmacol. 51(2), 215-219.
  • [31]. Ghasemi, P.A., Momeni, M., Bahmani, M. (2013). Ethnobotanical study of medicinal plants used by Kurd tribe in Dehloran and Abdanan districts, Ilam province, Iran. Afr J Tradit Complement Altern Med., 10(2), 368-385.
  • [32]. Serafini, M., Peluso, I., Raguzzini, A. (2010). Flavonoids as anti-inflammatory agents. Proc Nutr Soc., 69(3), 273-278.
There are 32 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Aybeniz Yıldırım 0000-0002-5801-4726

Ali Şen 0000-0002-2144-5741

Ahmet Doğan 0000-0003-0603-5100

Leyla Bitis This is me 0000-0003-1167-6666

Publication Date July 15, 2019
Submission Date January 8, 2019
Published in Issue Year 2019 Volume: 6 Issue: 2

Cite

APA Yıldırım, A., Şen, A., Doğan, A., Bitis, L. (2019). Antioxidant and anti-inflammatory activity of capitula, leaf and stem extracts of Tanacetum cilicicum (Boiss.) Grierson. International Journal of Secondary Metabolite, 6(2), 211-222. https://doi.org/10.21448/ijsm.510316

Cited By
















International Journal of Secondary Metabolite

e-ISSN: 2148-6905