In vitro and in silico Evaluation of Some Natural Molecules as Potent Glutathione Reductase Inhibitors

Year 2019, Volume: 6 Issue: 4, 310 - 316, 15.01.2020

Tuba Aydin

https://doi.org/10.21448/ijsm.628043

Cited By: 1

Abstract

Glutathione
reductase inhibitors are very popular antimalarial and anticancer agents. In
this study, in vitro inhibition effects of β-sitosterol,
stigmasterol, diosgenin and jervine which containing steroidal structure were
determined against glutathione reductase enzyme. β-sitosterol, diosgenin
and jervine were isolated from Veratrum album and stigmasterol was
isolated from Artemisia dracunculus L. by chromatographic methods.
According to the results obtained, IC₅₀ values of β-sitosterol,
stigmasterol, diosgenin and jervine were found as 1.2580, 5.2116, 0.1916 and
0.7701 µM, respectively. Among test compounds, diosgenin showed the strongest
inhibitory effect against glutathione reductase with Swissdock docking figure.
In current study first time, β-sitosterol, stigmasterol, diosgenin and
jervine were found to be much more glutathione reductase inhibitors.

Keywords

Secondary metabolites, Glutathione reductase, Inhibition, Docking

Thanks

The author gratefully thanks to Swissdock for data. The author faithfully thanks to Dr. Ahmet Cakir for supportings in the structure characterization and Dr. Murat Senturk for supportings in the enzyme inhibition researchs.

In vitro and in silico Evaluation of Some Natural Molecules as Potent Glutathione Reductase Inhibitors

Abstract

Glutathione reductase inhibitors are very popular antimalarial and anticancer agents. In this study, in vitro inhibition effects of β-sitosterol, stigmasterol, diosgenin and jervine which containing steroidal structure were determined against glutathione reductase enzyme. β-sitosterol, diosgenin and jervine were isolated from Veratrum album and stigmasterol was isolated from Artemisia dracunculus L. by chromatographic methods. According to the results obtained, IC50 values of β-sitosterol, stigmasterol, diosgenin and jervine were found as 1.2580, 5.2116, 0.1916 and 0.7701 µM, respectively. Among test compounds, diosgenin showed the strongest inhibitory effect against glutathione reductase with Swissdock docking figure. In current study first time, β-sitosterol, stigmasterol, diosgenin and jervine were found to be much more glutathione reductase inhibitors.

Keywords

Secondary metabolites, Glutathione reductase, Inhibition, Docking

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