Phytochemistry and Antioxidant Activities of the Methanolic Extract Obtained from the Leaves of Citrus limon (L.) Osbeck

Year 2020, Volume: 7 Issue: 1, 47 - 53, 20.03.2020

Bouzenna Hafsia Dhibi Sabah Samout Noura Elfeki Abdelfattah Hfaiedh Najla

https://doi.org/10.21448/ijsm.590522

Abstract

This study was aimed to identify the phytochemistry and antioxidant activities of the methanol extract obtained from the leaves of Citrus limon (L.) Osbeck (CLM). HPLC analyses revealed the presence of various phenolics (gallic acid, catechin, vanillic acid, coumaric, resveratrol) and flavonoids (rutin and apigenin).Qualitative analyses showed phenolics (302.91 μg EAG/mg), tannins (36.86 ± 0.71μg ECT/mg) and flavonoids (19.77 ± 0.06 μg EQ/mg) and coumarins. CLM exhibited a significant concentration-dependent in vitro antioxidant activity against DPPH radical and reducing power (FRAP test). This study concluded that the methanol extract obtained from the leaves of C. limon possess an antioxidant potential.

Keywords

Citrus limon L., Antioxidant Activity, HPLC, Phenolics, Flavonoid

References

• Sofowora, A., Ogunbodede, E., Onayade, A. (2013). The Role and Place of Medicinal Plants in the Strategies for Disease Prevention. Afr J Tradit Complement Altern Med., 10 (5), 210-229.
• Solecki, R., Shanidar, I.V. (1975). Aneanderthal Flower Burial in Northern Iraq. Science, 190(4217), 880-881.
• Shoemaker, M., Hamilton, B., Dairkee, S.H., Cohen, I., Campbell, M.J. (2005). In vitro Anticancer Activity of Twelve Chinese Medicinal Herbs. Phytother. Res., 19, 649-651.
• Farnsworth, N.R., Akerele, O., Bingel, A.S., Soejarto, D.D., Guo, Z. (1986). Places des plantes médicinales dans la thérapeutique. Bulletin de l'organisation mondiale de la santé., 64(2), 159-164.
• Diab, K. (2016). In Vitro studies on Phytochemical Content, Antioxidant, Anticancer, Immunomodulatory, and Antigenotoxic Activities. Asian Pac J Cancer Prev., 17(7), 3559-3567.
• Wu, T., Luo, J., Xu B. (2015). In vitro Antidiabetic Effects of Selected Fruits and Vegetables Against Glycosidase and Aldose Reductase. Food Science and Nutrition., 3(6), 459-505.
• Belletti, N., Ndagijimana, M., Sisto, C., Guerzoni, M. E., Lanciotti, R., Gardini, F. (2004). Evaluation of the Antimicrobial Activity of Citrus Essences on Saccharomy Cescerevisiae. J. Agric. Food Chem., 52, 6932-6938.
• Jing, L., Lei, Z., Li, L., Xie, R., Xi, W., Guan, Y., Zhou, Z. (2014). Antifungal Activity of Citrus Essential Oils. J Agric Food Chem., 62(14), 3011-3033.
• Bouzenna, H., Dhibi, S., Samout, N., Rjeibi, I., Talarmin, H., Elfeki, A., Hfaiedh, N. (2016). The Protective Effect of Citrus Limon Essential Oil on Hepatotoxicity and Nephrotoxicity Induced by Aspirin in Rats. Biomed. Pharmacother., 83, 1327-1334.
• Bouzenna, H., Hfaiedh, N., Giroux-Metges, M-A., Elfeki, A., Talarmin, H. (2017). Protective Effects of Essential Oil of Citrus Limon against Aspirin-Induced Toxicity in IEC-6 Cells. Appl Physiol Nutr Metab., 15, 479-486.
• Bouzenna, H., Samout, N., Dhibi, S., Mbarki, S., Akermi, S., Khdhiri, A., Elfeki, A., Hfaiedh, N. (2018). Protective Effect of Essential Oil from Citrus Limon against Aspirin-Induced Toxicity in Rats. Hum Exp Toxicol., 1-11.
• Ravishankara, M.N., Shrivastava, N., Padh, H., Rajani, M. (2002). Evaluation of Antioxidant Properties of Root Bark of Hemidesmes indicus. Phytomedicine. 9, 153-60.
• Singleton, V.L., Rossi, J.A. (1965). Colorimetry of Total Phenolics with Phosphomolybdic Phosphotungstic Acid Reagents. Am j Enol Viticult., 16, 144-158.
• Dewanto, V., Wu, X., Adom, K.K., Liu, R.H. (2002). Thermal Processing Enhances the Nutritional Value of Tomatoes by Increasing Total Antioxidant Activity. J Agric Food Chem., 50 (10), 3010-3014.
• Heimler, D., Vignolini, P., Dini, M. G., Vincieri, F., Rmani, A. (2006). Antiradical Activity and Polyphenol Composition of Local Brassicaceae Edible Varieties. Food Chemistry, 99, 464-469.
• Blois, M.S. (1958). Antioxidant Determinations by the Use of a Stable Free Radical. Nature. 26, 1199-1200.
• Chu, Y.H., Chang, C.L., Hsu, H.F. (2000). Flavonoid Content of Several Vegetables and Their Antioxidant Activity. J. Sci. Food Agric., 80, 561–566.
• Mcharek, N., Hanchi, B. (2017). Maturational Effects on Phenolic Constituents, Antioxidant Activities and LC-MS/MS Profiles of Lemon (Citrus limon) Peels. J Appl Bot Food Qual., 90, 1-9.
• Bocco, A., Cuvelier, M. E., Richard, H., Berset, C. (1998). Antioxidant Activity and Phenolic Composition of Citrus Peel and Seed Extracts. J Agric Food Chem., 46(6), 2123-2129.
• Tumane, P., Meshram, V., Wasnik, D. (2014). Comparative Study of Antibacterial Activity of Peel Extracts of Citrus Aurantium L. (Bitter orange) and Citrus medica L. (Lemon) Against Clinical Isolates from Wound Infection. Int J Pharm Bio Sci., 5(1), 382-7.
• Levizou, E., Drilias, P., Kyparissis, A. (2004). Exceptional Photosynthetic Performance of Capparisspinosa L. Under Adverse Conditions of Mediterranean Summer. Photosynthetica., 42(2), 229-235.