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Phytochemical Characterization and Biological Activities of Nepeta cadmea Boiss.

Year 2020, Volume 7, Issue 1, 28 - 34, 20.03.2020
https://doi.org/10.21448/ijsm.675128

Abstract

The genus Nepeta (Lamiaceae) is represented by 39 species, in total 50 taxa, 19 of which are endemic, in Turkey. Nepeta species are known as “kedinanesi” in Turkish and has many traditional usages such as antispasmodic, diuretic, antiseptic, antitussive, and antiasthmatic activities. The aerial parts of N. cadmea were hydrodistillated for 3 hours by using Clevenger apparatus to gather essential oil. To determine its chemical characterization, the essential oil was analyzed by GC-FID and GC-MS, simultaneously. 4aα,7α,7aβ-Nepetalactone (74.0%), 4aα,7α,7aα-nepetalactone (4.5%) and caryophyllene oxide (2.5%) were found as major components for essential oil. The potential in vitro antibacterial activity of the essential oil was evaluated using the broth microdilution assay. A panel of human pathogenic strains Escherichia coli NRRL B-3008, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 13311, Bacillus cereus NRRL B-3711 and Streptococcus sanguinis ATCC 10556 were used. Minimal Inhibitory Concentrations (MIC) of the samples were determined, where in ciprofloxacin was used as a positive control in the assay. MIC values were found 2500, 1000, 600, 600, 600 μg/mL against E. coli, P. aeruginosa, S. typhimurium, B. cereus and S. sanguinis, respectively. Compared the literature, it was seen that the essential oil had lower effective against these strains.

References

  • The Plant List (2013). Version 1.1. Available online: http://www.theplantlist.org/ (accessed on 29 October 2019).
  • Hedge, I.C. (1986). Labiatae of South-West Asia: Diversity, Distribution and Endemism. P Roy Soc B-Biol Sci, 89, 23-35. doi:10.1017/S0269727000008873
  • Davis, P.H. (1982). Flora of Turkey and the East Aegean Islands, Volume 7; Edinburgh University Press, Edinburgh, UK, pp. 264-288; ISBN 978-085-2243-96-1.
  • Aytaç, Z., Yıldız, G. (1996). A new record for the Flora of Turkey. Turk J Bot, 20(4), 385-387.
  • Dirmenci, T. (2012). Nepeta. In: Türkiye Bitkileri Listesi (Damarlı Bitkiler). Edits., Güner, A., Aslan, S., Ekim, T., Vural, M.and Babaç, M.T., Eds.; Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul, Türkiye, 2012; pp. 564-568, ISBN: 978-605-60425-7-7.
  • Dirmenci, T. (2003). Nepeta cadmea Boiss. ile Nepeta sulfuriflora P.H. Davis Türlerinin Morfolojik Olarak Karşılaştırılması. BAÜ Fen Bil. Enst. Dergisi, 5(2), 38-46.
  • Baytop, T. (1999). Türkiye’de Bitkiler ile Tedavi, Geçmişte ve Bugün, 2. baskı; Nobel Tıp Kitabevleri, İstanbul, Türkiye, pp. 480; ISBN 975-420-0211.
  • Tuzlacı, E. (2016). Türkiye Bitkileri Geleneksel İlaç Rehberi, İstanbul Tıp Kitabevleri, İstanbul, Türkiye, pp. 373; ISBN 978-605-4949-71-7.
  • Kaya, A., Dirmenci, T. (2008). Nutlet Surface Micromorphology of The Genus Nepeta L. (Lamiaceae) in Turkey. Turk J Bot, 32(2), 103-112.
  • Yılmaz, A., Boğa, M., Topçu, G. (2016). Novel Terpenoids with Potential Anti-Alzheimer Activity from Nepeta obtusicrena. Rec Nat Prod, 10(5), 530-541.
  • Kaska, A., Deniz, N., Çiçek, M., Mammadov, R. (2018). Evaluation of Antioxidant Properties, Phenolic Compounds, Anthelmintic and Cytotoxic Activities of Various Extracts Isolated from Nepeta Cadmea: An Endemic Plant for Turkey. J. Food Sci., 83(6), 1552-1559. doi: 10.1111/1750-3841.14167
  • Sammataro, D., Avitabile, A. (1998). The Beekeeper’s Handbook, 3rd ed.; Cornell University Press: USA, 1998; p. 8; ISBN 0-8014-8503-7.
  • Saraç, N., Uğur, A. (2009). The in vitro Antimicrobial Activities of The Essential Oils of Some Lamiaceae Species from Turkey. J. Med Food, 12(4), 902-907. doi:10.1089/jmf.2008.0089
  • Zomorodian, K., Saharkhiz, M.J., Shariati, S., Pakshir, K., Rahimi, M.J., Reza Khashei, R. (2012). Chemical Composition and Antimicrobial Activities of Essential Oils from Nepeta Cataria L. Against Common Causes of Food-Borne Infections. International Scholarly Research Notices, (Article ID 591953), 1-6. doi: 10.5402/2012/591953
  • Yavuz, C., Kılıç, D.D.D., Ayar, A., Yıldırım, T. (2017). Antibacterial Effects of Methanol Extracts of Some Plant Species Belonging to Lamiaceae Family. Int J Sec Metabolite, 4(3, special issue 2), 429-433. doi: 10.21448/ijsm.376691.
  • Demirci, F., Güven, K., Demirci, B., Dadandı, M.Y., Başer, K.H.C. (2008). Antibacterial Activity of Two Phlomis Essential Oils Against Food Pathogens. Food Control, 19(12), 1159-1164. doi: 10.1016/j.foodcont.2008.01.001
  • McLafferty, W., Stauffer, D.B. (1989). The Wiley/NBS Registry of Mass Spectral Data, J. Wiley and Sons, New York, USA.
  • Hochmuth, D.H. (2008). MassFinder 4.0, Hochmuth Scientific Consulting, Hamburg, Germany.
  • Joulain, D., Konig, W.A. (1998). The Atlas of Spectral Data of Sesquiterpene Hydrocarbons. J Nat Prod, 62(8), 1212-1213. doi:10.1021/np990755n
  • CLSI-Clinical and Laboratory Standards Institute M27-A2 (2002). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard-Second Edition, CLSI document 22(15).
  • Başer, K.H.C., Demirçakmak, B., Altıntaş, A., Duman, H. (1998). Composition of the Essential Oils of Nepeta Cadmea Boiss. J Essent Oil Res, 10(3), 327-328. doi:10.1080/10412905.1998.9700911
  • Başer, K.H.C., Kırımer, N., Kürkçüoğlu, M., Demirci, B. (2000). Essential Oils of Nepeta Species Growing in Turkey. Chem Nat Compd, 36(4), 356-359. doi:10.1023/A:1002832628159
  • Çelik, A., Mercan, N, Arslan, İ. Davran, H. (2008). Chemical Composition and Antimicrobial Activity of Essential Oil from Nepeta Cadmea. Chem Nat Compd, 44(1), 119-120. doi:10.1007/s10600-008-0036-6
  • Öz, E., Koç, S., Çinbilgel, İ., Yanıkoğlu, A., Çetin, H. (2018). Chemical Composition and Larvicidal Activity of Essential Oils from Nepeta cadmea Boiss. and Pimpinella Anisum L. on The Larvae of Culex pipiens L. Marmara Pharm J, 22(2), 322-327. doi:10.12991/mpj.2018.70

Phytochemical Characterization and Biological Activities of Nepeta cadmea Boiss.

Year 2020, Volume 7, Issue 1, 28 - 34, 20.03.2020
https://doi.org/10.21448/ijsm.675128

Abstract

The genus Nepeta (Lamiaceae) is represented by 39 species, in total 50 taxa, 19 of which are endemic, in Turkey. Nepeta species are known as “kedinanesi” in Turkish and has many traditional usages such as antispasmodic, diuretic, antiseptic, antitussive, and antiasthmatic activities. The aerial parts of N. cadmea were hydrodistillated for 3 hours by using Clevenger apparatus to gather essential oil. To determine its chemical characterization, the essential oil was analyzed by GC-FID and GC-MS, simultaneously. 4aα,7α,7aβ-Nepetalactone (74.0%), 4aα,7α,7aα-nepetalactone (4.5%) and caryophyllene oxide (2.5%) were found as major components for essential oil. The potential in vitro antibacterial activity of the essential oil was evaluated using the broth microdilution assay. A panel of human pathogenic strains Escherichia coli NRRL B-3008, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 13311, Bacillus cereus NRRL B-3711 and Streptococcus sanguinis ATCC 10556 were used. Minimal Inhibitory Concentrations (MIC) of the samples were determined, where in ciprofloxacin was used as a positive control in the assay. MIC values were found 2500, 1000, 600, 600, 600 μg/mL against E. coli, P. aeruginosa, S. typhimurium, B. cereus and S. sanguinis, respectively. Compared the literature, it was seen that the essential oil had lower effective against these strains.

References

  • The Plant List (2013). Version 1.1. Available online: http://www.theplantlist.org/ (accessed on 29 October 2019).
  • Hedge, I.C. (1986). Labiatae of South-West Asia: Diversity, Distribution and Endemism. P Roy Soc B-Biol Sci, 89, 23-35. doi:10.1017/S0269727000008873
  • Davis, P.H. (1982). Flora of Turkey and the East Aegean Islands, Volume 7; Edinburgh University Press, Edinburgh, UK, pp. 264-288; ISBN 978-085-2243-96-1.
  • Aytaç, Z., Yıldız, G. (1996). A new record for the Flora of Turkey. Turk J Bot, 20(4), 385-387.
  • Dirmenci, T. (2012). Nepeta. In: Türkiye Bitkileri Listesi (Damarlı Bitkiler). Edits., Güner, A., Aslan, S., Ekim, T., Vural, M.and Babaç, M.T., Eds.; Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul, Türkiye, 2012; pp. 564-568, ISBN: 978-605-60425-7-7.
  • Dirmenci, T. (2003). Nepeta cadmea Boiss. ile Nepeta sulfuriflora P.H. Davis Türlerinin Morfolojik Olarak Karşılaştırılması. BAÜ Fen Bil. Enst. Dergisi, 5(2), 38-46.
  • Baytop, T. (1999). Türkiye’de Bitkiler ile Tedavi, Geçmişte ve Bugün, 2. baskı; Nobel Tıp Kitabevleri, İstanbul, Türkiye, pp. 480; ISBN 975-420-0211.
  • Tuzlacı, E. (2016). Türkiye Bitkileri Geleneksel İlaç Rehberi, İstanbul Tıp Kitabevleri, İstanbul, Türkiye, pp. 373; ISBN 978-605-4949-71-7.
  • Kaya, A., Dirmenci, T. (2008). Nutlet Surface Micromorphology of The Genus Nepeta L. (Lamiaceae) in Turkey. Turk J Bot, 32(2), 103-112.
  • Yılmaz, A., Boğa, M., Topçu, G. (2016). Novel Terpenoids with Potential Anti-Alzheimer Activity from Nepeta obtusicrena. Rec Nat Prod, 10(5), 530-541.
  • Kaska, A., Deniz, N., Çiçek, M., Mammadov, R. (2018). Evaluation of Antioxidant Properties, Phenolic Compounds, Anthelmintic and Cytotoxic Activities of Various Extracts Isolated from Nepeta Cadmea: An Endemic Plant for Turkey. J. Food Sci., 83(6), 1552-1559. doi: 10.1111/1750-3841.14167
  • Sammataro, D., Avitabile, A. (1998). The Beekeeper’s Handbook, 3rd ed.; Cornell University Press: USA, 1998; p. 8; ISBN 0-8014-8503-7.
  • Saraç, N., Uğur, A. (2009). The in vitro Antimicrobial Activities of The Essential Oils of Some Lamiaceae Species from Turkey. J. Med Food, 12(4), 902-907. doi:10.1089/jmf.2008.0089
  • Zomorodian, K., Saharkhiz, M.J., Shariati, S., Pakshir, K., Rahimi, M.J., Reza Khashei, R. (2012). Chemical Composition and Antimicrobial Activities of Essential Oils from Nepeta Cataria L. Against Common Causes of Food-Borne Infections. International Scholarly Research Notices, (Article ID 591953), 1-6. doi: 10.5402/2012/591953
  • Yavuz, C., Kılıç, D.D.D., Ayar, A., Yıldırım, T. (2017). Antibacterial Effects of Methanol Extracts of Some Plant Species Belonging to Lamiaceae Family. Int J Sec Metabolite, 4(3, special issue 2), 429-433. doi: 10.21448/ijsm.376691.
  • Demirci, F., Güven, K., Demirci, B., Dadandı, M.Y., Başer, K.H.C. (2008). Antibacterial Activity of Two Phlomis Essential Oils Against Food Pathogens. Food Control, 19(12), 1159-1164. doi: 10.1016/j.foodcont.2008.01.001
  • McLafferty, W., Stauffer, D.B. (1989). The Wiley/NBS Registry of Mass Spectral Data, J. Wiley and Sons, New York, USA.
  • Hochmuth, D.H. (2008). MassFinder 4.0, Hochmuth Scientific Consulting, Hamburg, Germany.
  • Joulain, D., Konig, W.A. (1998). The Atlas of Spectral Data of Sesquiterpene Hydrocarbons. J Nat Prod, 62(8), 1212-1213. doi:10.1021/np990755n
  • CLSI-Clinical and Laboratory Standards Institute M27-A2 (2002). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard-Second Edition, CLSI document 22(15).
  • Başer, K.H.C., Demirçakmak, B., Altıntaş, A., Duman, H. (1998). Composition of the Essential Oils of Nepeta Cadmea Boiss. J Essent Oil Res, 10(3), 327-328. doi:10.1080/10412905.1998.9700911
  • Başer, K.H.C., Kırımer, N., Kürkçüoğlu, M., Demirci, B. (2000). Essential Oils of Nepeta Species Growing in Turkey. Chem Nat Compd, 36(4), 356-359. doi:10.1023/A:1002832628159
  • Çelik, A., Mercan, N, Arslan, İ. Davran, H. (2008). Chemical Composition and Antimicrobial Activity of Essential Oil from Nepeta Cadmea. Chem Nat Compd, 44(1), 119-120. doi:10.1007/s10600-008-0036-6
  • Öz, E., Koç, S., Çinbilgel, İ., Yanıkoğlu, A., Çetin, H. (2018). Chemical Composition and Larvicidal Activity of Essential Oils from Nepeta cadmea Boiss. and Pimpinella Anisum L. on The Larvae of Culex pipiens L. Marmara Pharm J, 22(2), 322-327. doi:10.12991/mpj.2018.70

Details

Primary Language English
Subjects Biology
Published Date March
Journal Section Articles
Authors

Gülderen YILMAZ>
ANKARA ÜNİVERSİTESİ
0000-0002-6569-4766
Türkiye


Gözde ÖZTÜRK>
Anadolu Üniversitesi
0000-0002-3998-8859
Türkiye


Mehmet ÇİÇEK> (Primary Author)
Pamukkale Üniversitesi
0000-0003-4058-3717
Türkiye


Betül DEMİRCİ>
Anadolu Üniversitesi
0000-0003-2343-746X
Türkiye

Publication Date March 20, 2020
Published in Issue Year 2020, Volume 7, Issue 1

Cite

APA Yılmaz, G. , Öztürk, G. , Çiçek, M. & Demirci, B. (2020). Phytochemical Characterization and Biological Activities of Nepeta cadmea Boiss. . International Journal of Secondary Metabolite , 7 (1) , 28-34 . DOI: 10.21448/ijsm.675128

International Journal of Secondary Metabolite (IJSM)

ISSN-e: 2148-6905