Callus Induction and Micropropagation of Lilium candidum L. Using Stem Bulbils and Confirmation of Genetic Stability via SSR-PCR

Year 2020, Volume: 7 Issue: 4, 286 - 296, 15.12.2020

Hilal Büşra Tokgöz , Filiz Altan

https://doi.org/10.21448/ijsm.753053

Cited By: 5

Abstract

Natural populations of Lilium candidum L. are remarkably affected by biotic and abiotic factors therefore there is a requirement to develop effective micropropagation protocol to provide mass production, multiplication and conservation of these plants. For this reason, this study was aimed to develop an efficient micropropagation method for multiple shoot production via somatic embryogenesis induced from L. candidum stem bulbils and also to determine the genetic stability of in vitro grown plants using SSR markers. The obtained results of this study are the first comprehensive reports including an investigation of genetic fidelity on somatic embryogenesis of L. candidum. After surface sterilization of bulbils, the calculated regeneration percentage of them was 89.5% and the callus induction was achieved using leaf segments of in vitro grown bulbils. The well formed somatic embryos were obtained from smooth whitish-yellow colored calli and these somatic embryos produced well formed healthy L. candidum seedlings with adventitious roots. All rooted seedlings were easily adapted to greenhouse conditions and the genetic stability of in vitro grown seedlings were determined by using SSR-PCR technique and it was calculated as 100%.

Keywords

Callus Culture, Genetic Stability, Lilium candidum L., Somatic Embryogenesis, SSR

Supporting Institution

The Scientific and Technological Resarch Council of Turkey (TUBİTAK)

Project Number

TOVAG- 217O009

Thanks

We thank to Assoc. Prof. Dr. Ergun KAYA for support and we also thank to Assoc. Prof. Dr. Hasan YILDIRIM and Assist Prof. Dr. Ademi PİRHAN for material support.

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