Research Article
BibTex RIS Cite

In vitro production of tropane alkaloids from Brugmansia suaveolens

Year 2022, Volume: 9 Issue: 1, 1 - 13, 10.03.2022
https://doi.org/10.21448/ijsm.934222

Abstract

For thousands of years, secondary metabolites have been utilized as medications, flavors, pesticides, and dyes. For the generation of valuable secondary metabolites, in vitro plant culture techniques have been viewed as beneficial alternatives to whole plants. Brugmansia suaveolens is an ornamental plant including anticholinergic agents which are employed in medicine. Callus induction was performed from leaf and internode explants cultured on Murashige and Skoog’s medium supplemented with different concentrations and combinations of plant growth regulators (PGRs) with 6 treatments. The highest callus induction response was obtained from the leaf-originated explants (73%) on the medium supplemented with 0.4 mg/L KIN and 0.2 mg/L NAA which produced friable callus in 4 weeks. The cell suspension culture of B. suaveolens was established in shake flasks using friable calli. The extraction protocol of tropane alkaloids was optimized, atropine and scopolamine were obtained efficiently. The data could provide technical support for the large-scale production of valuable alkaloids of B. suaveolens in vitro systems with improved strategies.

Supporting Institution

TUBITAK

Project Number

117H001

References

  • Alves, M.N., Sartoratto, A., & Trigo J.R. (2007). Scopolamine in Brugmansia Suaveolens (Solanaceae): Defense, Allocation, Costs, and Induced Response. J Chem. Ecol., 33, 297-309.
  • Anthony, S.J., Zuchowski, W., & Setzer, W.N. (2009). Composition of the floral essential oil of Brugmansia suaveolens. Rec. Nat. Prod., 3, 76–81.
  • Baque, A., Moh, S. Lee, E., et al. (2012). Production of biomass and useful compounds from adventitious roots of high-value added medicinal plants using bioreactor. Biotechnol. Adv., 30, 1255-1267.
  • Cardillo, A.B., Giulietti, A.M., Palazón, J. et al., (2013). Influence of hairy root ecotypes on production of tropane alkaloids in Brugmansia candida. Plant Cell Tiss. Organ Cult., 114, 305–312.
  • Chandran, H., Meena, M., Barupal, T., & Sharma, K. (2020). Plant tissue culture as a perpetual source for production of industrially important bioactive compounds Biotechnology Reports, 26, e00450. https://doi.org/10.1016/j.btre.2020.e00450
  • Dandin, V.S., & Murth, H.N. (2012). Enhanced in vitro multiplication of Nothapodytes nimmoniana Graham using semisolid and liquid cultures and estimation of camptothecin in the regenerated plants. Acta Physiol. Plant., 34, 1381–1386.
  • Dehghan, E., Hakkinen, S.T., Oksman-Caldentey, K.M., & Ahmadi, F.S. (2012). Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.). Plant Cell Tissue Organ Cult., 110, 35–44.
  • Dong, Y.S., Fu, C.H., Su, P., et al. (2015). Mechanisms and effective control of physiological browning phenomena in plant cell cultures. Physiol. Plant., 156, 13-28.
  • Ikeuchi, M., Sugimoto, K., & Iwase, A. (2013). Plant callus: mechanisms of induction and repression. Plant Cell, 25, 3159-3173.
  • Ghorbanpour, M., Omidi, M., Etminan A., Hatami, M., & Shooshtari, L. (2013). In Vitro Hyoscyamine and Scopolamine Production of Black Henbane (Hyoscyamus niger) from shoot tip culture under various plant growth regulators and aulture media. J. Trakia Science., 2, 125-134. Corpus ID: 30713657.
  • Kamada, H., Okamura, N., Satake, M., Harada, H. & and Shimomura, K. (1986). Alkaloid production by hairy root cultures in Atropa belladonna. Plant Cell Rep., 5, 239-242. https://doi.org/10.1007/BF00269811
  • Koetz, M., Santos, T.G., Rayane, M., & Henriques, A.T. (2017). Quantification of atropine in leaves of Atropa belladonna: development and validation of method by High Perfomance Liquid Chromatography. Drug Analytical Research, 1, 44 49. https://doi.org/10.22456/2527-2616.74150
  • Lee, C.W.T., & Shuler, M.L. (2000). The effect of inoculum density and conditioned medium on the production of ajmalicine and catharanthine from immobilized Catharanthus roseus cells. Biotechnol. Bioengr., 67, 61-71.
  • Liu, J., Feng. H., & Ma, Y. (2018). Effects of different plant hormones on callus induction and plant regeneration of miniature roses (Rosa hybrida L.). Horticult Int J., 2, 201-206. https://doi.org/10.15406/hij.2018.02.00053
  • Montanucci, C.A.R., Furlan, F., Neiverth, A.A., Neiverth, et al. (2012). Evaluation of seed germination and plant regeneration in Brugmansia suaveolens- a tropane alkaloid producer plant. International Journal of Medicinal and Aromatic Plants, 2, 396-405.
  • Murashige. T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant, 15, 473–497.
  • Pitta-Alvarez, S., Spollansky, T., & Giulietti, A. (2000). The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzyme Microb. Technol., 26, 252 258. https://doi.org/10.1023/A:1005638029034
  • Praveen, N., Naik, P.M., Manohar, S.H. et al. (2009). In vitro regeneration of brahmi shoots using semisolid and liquid cultures and quantitative analysis of bacoside A. Acta Physiol Plant, 31, 723–728.
  • Sarin, R. (2005). Useful Metabolites from Plant Tissue Cultures. Biotechnology, 4, 79-93. https://scialert.net/abstract/doi=biotech.2005.79.93

In vitro production of tropane alkaloids from Brugmansia suaveolens

Year 2022, Volume: 9 Issue: 1, 1 - 13, 10.03.2022
https://doi.org/10.21448/ijsm.934222

Abstract

For thousands of years, secondary metabolites have been utilized as medications, flavors, pesticides, and dyes. For the generation of valuable secondary metabolites, in vitro plant culture techniques have been viewed as beneficial alternatives to whole plants. Brugmansia suaveolens is an ornamental plant including anticholinergic agents which are employed in medicine. Callus induction was performed from leaf and internode explants cultured on Murashige and Skoog’s medium supplemented with different concentrations and combinations of plant growth regulators (PGRs) with 6 treatments. The highest callus induction response was obtained from the leaf-originated explants (73%) on the medium supplemented with 0.4 mg/L KIN and 0.2 mg/L NAA which produced friable callus in 4 weeks. The cell suspension culture of B. suaveolens was established in shake flasks using friable calli. The extraction protocol of tropane alkaloids was optimized, atropine and scopolamine were obtained efficiently. The data could provide technical support for the large-scale production of valuable alkaloids of B. suaveolens in vitro systems with improved strategies.

Project Number

117H001

References

  • Alves, M.N., Sartoratto, A., & Trigo J.R. (2007). Scopolamine in Brugmansia Suaveolens (Solanaceae): Defense, Allocation, Costs, and Induced Response. J Chem. Ecol., 33, 297-309.
  • Anthony, S.J., Zuchowski, W., & Setzer, W.N. (2009). Composition of the floral essential oil of Brugmansia suaveolens. Rec. Nat. Prod., 3, 76–81.
  • Baque, A., Moh, S. Lee, E., et al. (2012). Production of biomass and useful compounds from adventitious roots of high-value added medicinal plants using bioreactor. Biotechnol. Adv., 30, 1255-1267.
  • Cardillo, A.B., Giulietti, A.M., Palazón, J. et al., (2013). Influence of hairy root ecotypes on production of tropane alkaloids in Brugmansia candida. Plant Cell Tiss. Organ Cult., 114, 305–312.
  • Chandran, H., Meena, M., Barupal, T., & Sharma, K. (2020). Plant tissue culture as a perpetual source for production of industrially important bioactive compounds Biotechnology Reports, 26, e00450. https://doi.org/10.1016/j.btre.2020.e00450
  • Dandin, V.S., & Murth, H.N. (2012). Enhanced in vitro multiplication of Nothapodytes nimmoniana Graham using semisolid and liquid cultures and estimation of camptothecin in the regenerated plants. Acta Physiol. Plant., 34, 1381–1386.
  • Dehghan, E., Hakkinen, S.T., Oksman-Caldentey, K.M., & Ahmadi, F.S. (2012). Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.). Plant Cell Tissue Organ Cult., 110, 35–44.
  • Dong, Y.S., Fu, C.H., Su, P., et al. (2015). Mechanisms and effective control of physiological browning phenomena in plant cell cultures. Physiol. Plant., 156, 13-28.
  • Ikeuchi, M., Sugimoto, K., & Iwase, A. (2013). Plant callus: mechanisms of induction and repression. Plant Cell, 25, 3159-3173.
  • Ghorbanpour, M., Omidi, M., Etminan A., Hatami, M., & Shooshtari, L. (2013). In Vitro Hyoscyamine and Scopolamine Production of Black Henbane (Hyoscyamus niger) from shoot tip culture under various plant growth regulators and aulture media. J. Trakia Science., 2, 125-134. Corpus ID: 30713657.
  • Kamada, H., Okamura, N., Satake, M., Harada, H. & and Shimomura, K. (1986). Alkaloid production by hairy root cultures in Atropa belladonna. Plant Cell Rep., 5, 239-242. https://doi.org/10.1007/BF00269811
  • Koetz, M., Santos, T.G., Rayane, M., & Henriques, A.T. (2017). Quantification of atropine in leaves of Atropa belladonna: development and validation of method by High Perfomance Liquid Chromatography. Drug Analytical Research, 1, 44 49. https://doi.org/10.22456/2527-2616.74150
  • Lee, C.W.T., & Shuler, M.L. (2000). The effect of inoculum density and conditioned medium on the production of ajmalicine and catharanthine from immobilized Catharanthus roseus cells. Biotechnol. Bioengr., 67, 61-71.
  • Liu, J., Feng. H., & Ma, Y. (2018). Effects of different plant hormones on callus induction and plant regeneration of miniature roses (Rosa hybrida L.). Horticult Int J., 2, 201-206. https://doi.org/10.15406/hij.2018.02.00053
  • Montanucci, C.A.R., Furlan, F., Neiverth, A.A., Neiverth, et al. (2012). Evaluation of seed germination and plant regeneration in Brugmansia suaveolens- a tropane alkaloid producer plant. International Journal of Medicinal and Aromatic Plants, 2, 396-405.
  • Murashige. T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant, 15, 473–497.
  • Pitta-Alvarez, S., Spollansky, T., & Giulietti, A. (2000). The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzyme Microb. Technol., 26, 252 258. https://doi.org/10.1023/A:1005638029034
  • Praveen, N., Naik, P.M., Manohar, S.H. et al. (2009). In vitro regeneration of brahmi shoots using semisolid and liquid cultures and quantitative analysis of bacoside A. Acta Physiol Plant, 31, 723–728.
  • Sarin, R. (2005). Useful Metabolites from Plant Tissue Cultures. Biotechnology, 4, 79-93. https://scialert.net/abstract/doi=biotech.2005.79.93
There are 19 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Tijen Talas Oğraş 0000-0001-6608-8728

Elif Tahtasakal

Selma Öztürk 0000-0002-7949-8993

Project Number 117H001
Publication Date March 10, 2022
Submission Date May 7, 2021
Published in Issue Year 2022 Volume: 9 Issue: 1

Cite

APA Talas Oğraş, T., Tahtasakal, E., & Öztürk, S. (2022). In vitro production of tropane alkaloids from Brugmansia suaveolens. International Journal of Secondary Metabolite, 9(1), 1-13. https://doi.org/10.21448/ijsm.934222
International Journal of Secondary Metabolite

e-ISSN: 2148-6905