Curvularia lunata: A fungus for possible berberine transformation

Year 2022, Volume: 9 Issue: 1, 66 - 73, 10.03.2022

Deniz Yılmaz , Fatma Gizem Avcı , Berna Sarıyar Akbulut

https://doi.org/10.21448/ijsm.996589

Cited By: 2

Abstract

The prevalence of multidrug-resistant microorganisms results in an urgent need for the development of new antimicrobial agents or new treatment strategies. In this sense, plants serve different alternatives. Berberine, a plant-derived compound, is one of the alkaloids known to display antimicrobial activity against several types of microorganisms, while its being a substrate of various efflux pumps causes a decrease in its efficacy. Biotransformation makes it possible to obtain novel or more effective compounds with only minor structural modifications using enzyme systems. In this study, biotransformation of berberine by Curvularia lunata was examined. The working concentration of berberine was determined by observing the microbial growth on agar plates. The concentration of residual berberine in the media was analyzed by HPLC. In addition, laccase and beta-glucosidase enzyme activities were followed for their possible roles during the biotransformation of berberine. The results show that at the end of 14 days, C. lunata consumed 99% and 87% of berberine with the initial concentrations of 0.35 mg/mL and 0.5 mg/mL, respectively. Enzyme activities were not affected significantly. Since the concentration of berberine decreased, the biotransformation of berberine by C. lunata could be mentioned. Monitoring of biotransformation products plays a crucial role in discovering novel antimicrobial compounds and new valuable molecules.

Keywords

Biotransformation, Berberine, Curvularia lunata, Antimicrobial resistance

Supporting Institution

Marmara University, Scientific Research Projects Committee

Project Number

FEN-C- 070317-0110

References

• Avci, F.G., Sayar, N.A., & Sariyar Akbulut, B. (2018). An OMIC approach to elaborate the antibacterial mechanisms of different alkaloids. Phytochemistry, 149, 123–131. https://doi.org/10.1016/j.phytochem.2017.12.023
• Banerjee, U. C. (1992). Immobilized beta-glucosidase from Curvularia lunata. Folia Microbiologica, 37(4), 256–260. https://doi.org/10.1007/BF02814559
• Bianchini, L.F., Arruda, M.F.C., Vieira, S.R., Campelo, P.M.S., Grégio, A.M.T., & Rosa, E.A.R. (2015). Microbial biotransformation to obtain new antifungals. Frontiers in Microbiology, 6, 1433. https://doi.org/10.3389/fmicb.2015.01433
• Bukvicki, D., Novaković, M., Ilić-Tomić, T., Nikodinović-Runić, J., Todorović, N., Veljić, M., & Asakawa, Y. (2021). Biotransformation of Perrottetin F by Aspergillus niger: New Bioactive Secondary Metabolites. Records of Natural Products, 15(4), 281-292. https://doi.org/10.25135/rnp.215.20.09.1812
• Collins, D.O., Buchanan, G.O., Reynolds, W.F., & Reese, P.B. (2001). Biotransformation of squamulosone by Curvularia lunata ATCC 12017. Phytochemistry, 57(3), 377–383. https://doi.org/10.1016/S0031-9422(01)00060-7
• Coman, C., Moţ, A.C., Gal, E., Pârvu, M. & Silaghi-Dumitrescu, R. (2013). Laccase is upregulated via stress pathways in the phytopathogenic fungus Sclerotinia sclerotiorum. Fungal Biology, 117(7–8), 528–539. https://doi.org/10.1016/j.funbio.2013.05.005
• Eliwa, D., Albadry, M. A., Ibrahim, A.R.S., Kabbash, A., Meepagala, K., Khan, I.A., El-Aasr, M., & Ross, S.A. (2021). Biotransformation of papaverine and in silico docking studies of the metabolites on human phosphodiesterase 10a. Phytochemistry, 183, 112598. https://doi.org/10.1016/j.phytochem.2020.112598
• Fura, A. (2006). Role of pharmacologically active metabolites in drug discovery and development. Drug Discovery Today, 11(3–4), 133–142. https://doi.org/10.1016/S1359-6446(05)03681-0
• Giri, A., Dhingra, V., Giri, C.C., Singh, A., Ward, O.P., & Narasu, M.L. (2001). Biotransformations using plant cells, organ cultures and enzyme systems: Current trends and future prospects. Biotechnology Advances, 19(3), 175–199. https://doi.org/10.1016/S0734-9750(01)00054-4
• Liu, J.H., & Yu, B.Y. (2010). Biotransformation of bioactive natural products for pharmaceutical lead compounds. Current Organic Chemistry, 14(14), 1400–1406. https://doi.org/10.2174/138527210791616786
• Pervaiz, I., Ahmad, S., Madni, M.A., Ahmad, H., & Khaliq, F.H. (2013). Microbial biotransformation: a tool for drug designing (Review). Prikladnaia Biokhimiia Mikrobiologiia, 49(5), 435–449. https://doi.org/10.7868/s0555109913050097
• Rozzell, J.D. (1999). Commercial scale biocatalysis: myths and realities. Bioorganic & Medicinal Chemistry, 7(10), 2253–2261. https://doi.org/10.1016/S0968-0896(99)00159-5
• Schmeda-Hirschmann, G., Astudillo, L., & Palenzuela, J.A. (2004). Biotransformation of solidagenone by Alternaria alternata, Aspergillus niger, and Curvularia lunata cultures. World Journal of Microbiology and Biotechnology, 20(1), 93 97. https://doi.org/10.1023/B:WIBI.0000013317.60257.33
• Singh, S.K., Khajuria, R., & Kaur, L. (2017). Biodegradation of ciprofloxacin by white rot fungus Pleurotus ostreatus. 3 Biotech, 7(1), 1–8. https://doi.org/10.1007/s13205-017-0684-y
• Sultana, N. (2018). Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation. Steroids, 136, 76–92. https://doi.org/10.1016/j.steroids.2018.01.007
• Venisetty, R., & Ciddi, V. (2003). Application of microbial biotransformation for the new drug discovery using natural drugs as substrates. Current Pharmaceutical Biotechnology, 4(3), 123–140. https://doi.org/10.2174/1389201033489847