Effect of fixation methods and various clones of Camellia sinensis var. sinensis (L) properties and antioxidant activity of Indonesian green tea

Year 2022, Volume: 9 Issue: 3, 278 - 289, 26.09.2022

M Iqbal Prawira-atmaja , Fadhilatul Ula Hilman Maulana Sugeng Harianto S Shabri Dede Zainal Arief

https://doi.org/10.21448/ijsm.1014894

Cited By: 3

Abstract

Fixation is essential in green tea processing to inactivate the polyphenol oxidase enzyme. In Indonesia, green tea is made from the Assam variety and produced using the panning method. Few studies are reported on green tea made from Indonesian clones of the Sinensis variety. This study aims to identify chemical characteristics, antioxidant activity, and sensory evaluation of green tea from local clones of the Sinensis variety (GMBS 2, GMBS 4, and GMBS 5) with different fixation methods (panning and steaming). The results show that the caffeine content of green tea products ranged from 2.51-2.59% and 2.67-2.74% for panning and steaming methods. The panning method produced green tea with higher total polyphenol and flavonoid content than the steaming method. Green tea with the panning method has an IC50 value of 14.45; 14.41; and 17.41 mg/L for GMBS 2, GMBS 4, and GMBS 5, respectively. The panning method resulted in a smaller IC50 value than the steaming method for GMBS 2 and GMBS 4 clones. The steaming method produced green tea with a higher taste, aroma, and total score than those the panning method. However, different fixation methods did not significantly affect the appearance, liquor color, and leaf infusion. In conclusion, different fixation methods on GMBS 2, GMB 4, and GMB 5 produced green tea products that met the Indonesian National Standard 3945:2016. Further research is needed to determine the role of the plucking period/season and the characteristics of volatile compounds of green tea from GMBS clones with different fixation methods.

Keywords

Fixation methods, Indonesian green tea, Sinensis variety, GMBS clones, Antioxidant activity

Supporting Institution

Indonesia Research Institute for Tea and Cinchona, Dep. Food Technology, Faculty of Engineering, Universitas Pasundan

Project Number

This research did not receive any specific grant from funding sources agencies.

Thanks

The authors would like to thank Adhi Irianto Mastur as Gambung Experimental Garden Manager for providing a block area experimental tea plantation. We are also grateful to Mr. Heri Syahrian, as tea breeder Researcher, for identifying GMBS clones.

References

• Adnan, M., Ahmad, A., Ahmed, A., Khalid, N., Hayat, I., & Ahmed, I. (2013). Chemical composition and sensory evaluation of tea (Camellia sinensis) commercialized in Pakistan. Pakistan Journal of Botany, 45(3), 901–907.
• Ahmed, S., & Stepp, J.R. (2013). Pu-erh Tea: Botany, Production, and Chemistry. In V.R. Preedy (Ed.), Tea in Health and Disease Prevention (pp. 59–71). Elsevier. https://doi.org/10.1016/B978-0-12-384937-3.00005-7
• Alam, K.M.M., Rashid, R.A.C., Uddin, M.S., & Chowdhury, M.A.M. (2015). Screening for Qualitative Status of Five Popular Marketed Brands of Tea Produced in Bangladesh. Universal Journal of Plant Science, 3(3), 43–48. https://doi.org/10.13189/ujps.2015.030301
• Anesini, C., Ferraro, G.E., & Filip, R. (2008). Total Polyphenol Content and Antioxidant Capacity of Commercially Available Tea (Camellia sinensis) in Argentina. Journal of Agricultural and Food Chemistry, 56(19), 9225–9229. https://doi.org/10.1021/jf8022782
• Aroyeun, S.O. (2013). Crude fibre, water extracts, total ash, caffeine and moisture contents as diagnostic factors in evaluating green tea quality. World Sustainable Development Outlook 2012, 25(1), 337–348.
• Balasooriya, R., Kooragoda, M., & Jayawardhane, P. (2019). Comparative analysis on physical and chemical characteristics of commercially manufactured/processed green tea in Sri Lanka. International Journal of Food Science and Nutrition, 4(4), 43 47. https://doi.org/10.13140/RG.2.2.11002.85441
• Balentine, D.A. (2000). Tea. In Kirk-Othmer Encyclopedia of Chemical Technology (Issue 1). John Wiley & Sons, Inc. https://doi.org/10.1002/0471238961.20050102011205.a01
• Carloni, P., Tiano, L., Padella, L., Bacchetti, T., Customu, C., Kay, A., & Damiani, E. (2013). Antioxidant activity of white, green and black tea obtained from the same tea cultivar. Food Research International, 53(2), 900–908. https://doi.org/10.1016/j.foodres.2012.07.057
• Chan, E.W.C., Lim, Y.Y., & Chew, Y.L. (2007). Antioxidant activity of Camellia sinensis leaves and tea from a lowland plantation in Malaysia. Food Chemistry, 102(4), 1214–1222. https://doi.org/10.1016/j.foodchem.2006.07.009
• Chaturvedula, V.S.P., & Prakash, I. (2011). The aroma, taste, color and bioactive constituents of tea. Journal of Medicinal Plants Research, 5(11), 2110–2124.
• de Oliveira, A., Prince, D., Lo, C.-Y., Lee, L.H., & Chu, T.-C. (2015). Antiviral activity of theaflavin digallate against herpes simplex virus type 1. Antiviral Research, 118(March), 56–67. https://doi.org/10.1016/j.antiviral.2015.03.009
• Diniz, P.H.G.D., Pistonesi, M.F., Alvarez, M.B., Band, B.S.F., & de Araújo, M.C.U. (2015). Simplified tea classification based on a reduced chemical composition profile via successive projections algorithm linear discriminant analysis (SPA-LDA). Journal of Food Composition and Analysis, 39(2015), 103–110. https://doi.org/10.1016/j.jfca.2014.11.012
• Engelhardt, U.H. (2010). Chemistry of tea. In Comprehensive Natural Products II: Chemistry and Biology (Vol. 3, pp. 999–1032). https://doi.org/10.1016/B978-008045382-8.00089-7
• Erol, N.T., Sarı, F., & Velioglu, Y.S. (2010). Polyphenols , Alkaloids and Antioxidant Activity of Different Grades Turkish Black Tea. Gıda, 35, 161 168. https://dergipark.org.tr/en/pub/gida/issue/6879/92067
• Faizasa, K.K., Koushki, M., & Haghighat, S.R. (2017). Physicochemical Properties, Microbial Quality and Sensory Attributes of Different Black Tea Brands. Current Nutrition & Food Science, 13(3), 212–218. https://doi.org/10.2174/1573401313666170126154654
• Hajiaghaalipour, F., Kanthimathi, M.S., Sanusi, J., & Rajarajeswaran, J. (2015). White tea (Camellia sinensis) inhibits proliferation of the colon cancer cell line, HT-29, activates caspases and protects DNA of normal cells against oxidative damage. Food Chemistry, 169, 401–410. https://doi.org/10.1016/j.foodchem.2014.07.005
• Hicks, M.B., Hsieh, Y.-H.P., & Bell, L.N. (1996). Tea preparation and its influence on methylxanthine concentration. Food Research International, 29(3 4), 325 330. https://doi.org/10.1016/0963-9969(96)00038-5
• Jayawardhane, S.A.D.P.S., Madushanka, K.P.C., Mewan, K.M., Jayasinghe, S.K., Karunajeewa, D.G.N.P., & Edirisinghe, E.N.U. (2016). Determination of Quality Characteristics in Different Green Tea Products Available in Sri Lankan Supermarkets. 6th Symposium on Plantation Crop Research, 57–68.
• Lin, S.-D., Udompornmongkol, P., Yang, J.-H., Chen, S.-Y., & Mau, J.-L. (2014). Quality and Antioxidant Property of Three Types of Tea Infusions. Journal of Food Processing and Preservation, 38(4), 1401–1408. https://doi.org/10.1111/jfpp.12099
• Lin, Y.-S., Tsai, Y.-J., Tsay, J.-S., & Lin, J.-K. (2003). Factors Affecting the Levels of Tea Polyphenols and Caffeine in Tea Leaves. Journal of Agricultural and Food Chemistry, 51(7), 1864–1873. https://doi.org/10.1021/jf021066b
• Martono, B., & Udarno, L. (2015). Caffeine Content and Characteristics of Shoot Morphology of Six Tea Genotypes. Jurnal Tanaman Industri Dan Penyegar, 2(2), 69. https://doi.org/10.21082/jtidp.v2n2.2015.p69-76
• Maulana, H., Prawira-Atmaja, M.I., Shabri, Hamdini, N., Alyanisa, J., Harianto, S., & Rohdiana, D. (2020). Changes of chemical contents during the withering process of white tea. IOP Conference Series: Earth and Environmental Science, 443(1), 012023. https://doi.org/10.1088/1755-1315/443/1/012023
• Nadiah, N.I., Cheng, L.H., Azhar, M.E., Karim, A.A., Uthumporn, U., & Ruri, A.S. (2015). Determination of phenolics and antioxidant properties in tea and the effects of polyphenols on alpha-amylase activity. Pakistan Journal of Nutrition, 14(11), 808–817. https://doi.org/10.3923/pjn.2015.808.817
• Owuor, P.O., & Chavanji, A.M. (1986). Caffeine contents of clonal tea; seasonal variations and effects of plucking standards under Kenyan conditions. Food Chemistry, 20(3), 225–233. https://doi.org/10.1016/0308-8146(86)90175-5
• Ozturk, B., Seyhan, F., Ozdemir, I. S., Karadeniz, B., Bahar, B., Ertas, E., & Ilgaz, S. (2016). Change of enzyme activity and quality during the processing of Turkish green tea. LWT - Food Science and Technology, 65, 318–324. https://doi.org/10.1016/j.lwt.2015.07.068
• Paiva, L., Rego, C., Lima, E., Marcone, M., & Baptista, J. (2021). Comparative analysis of the polyphenols, caffeine, and antioxidant activities of green tea, white tea, and flowers from azorean camellia sinensis varieties affected by different harvested and processing conditions. Antioxidants, 10(2), 1–16. https://doi.org/10.3390/antiox10020183
• Prawira-Atmaja, M.I., Azhary, B., Harianto, S., Maulana, H., Shabri, & Rohdiana, D. (2019). Total Polyphenol, Rehydration Ratio, and Liquor Color of Different Grade Green Tea. Jurnal Ilmu Pangan Dan Hasil Pertanian, 3(2), 159 169. https://doi.org/10.26877/jiphp.v3i2.5116
• Prawira-Atmaja, M.I., Maulana, H., Shabri, S., Riski, G.P., Fauziah, A., Harianto, S., & Rohdiana, D. (2021). Evaluation of the conformity of the quality of tea products with the requirements of the Indonesian National Standard. Jurnal Standardisasi, 23(1), 43. https://doi.org/10.31153/js.v23i1.845
• Prawira-Atmaja, M.I., Shabri, S., Khomaini, H.S., Maulana, H., Harianto, S., & Rohdiana, D. (2018). Changes in chlorophyll and polyphenols content in Camellia sinensis var. sinensis at different stage of leaf maturity. IOP Conference Series: Earth and Environmental Science, 131(1). https://doi.org/10.1088/1755-1315/131/1/012010
• Prayoga, M.K., Syahrian, H., Rahadi, V.P., Prawira-Atmaja, M.I., Maulana, H., & Anas, A. (2022). Quality diversity of 35 tea clones (Camellia sinensis var. sinensis) processed for green tea. Biodiversitas Journal of Biological Diversity, 23(2), 810 816. https://doi.org/10.13057/biodiv/d230227
• Reygaert, W. (2017). An Update on the Health Benefits of Green Tea. Beverages, 3(4), 6. https://doi.org/10.3390/beverages3010006
• Roshanak, S., Rahimmalek, M., & Goli, S.A.H. (2016). Evaluation of seven different drying treatments in respect to total flavonoid, phenolic, vitamin C content, chlorophyll, antioxidant activity and color of green tea (Camellia sinensis or C. assamica) leaves. Journal of Food Science and Technology, 53(1), 721–729. https://doi.org/10.1007/s13197-015-2030-x
• Sharangi, A.B., Siddiqui, W., & Dávila Aviña, J.E. (2014). Black Tea Magic: Overview of Global Research on Human Health and Therapeutic Potentialities. Journal of Tea Science Research, 4(1), 1–16. https://doi.org/10.5376/jtsr.2014.04.0001
• Sharma, P.K., Ali, M., & Yadav, D.K. (2011). Physicochemical and phytochemical evaluation of different black tea brands. Journal of Applied Pharmaceutical Science, 1(3), 121–124.
• Skotnicka, M., Chorostowska-Wynimko, J., Jankun, J., & Skrzypczak-Jankun, E. (2011). The black tea bioactivity: An overview. Central-European Journal of Immunology, 36(4), 284–292.
• Suprihatini, R. (2015). Supply chain analysis of Indonesia tea. Jurnal Penelitian Teh Dan Kina, 18(2), 107–118. https://doi.org/10.22302/pptk.jur.jptk.v18i2.72
• Temple, S.J., & van Boxtel, A.J.B. (1999). Thin Layer Drying of Black Tea. Journal of Agricultural Engineering Research, 74(2), 167–176. https://doi.org/10.1006/jaer.1999.0448
• Teshome, K. (2019). Effect of tea processing methods on biochemical composition and sensory quality of black tea (Camellia sinensis (L.) O . Kuntze): A review. Journal of Horticulture and Forestry, 11(6), 84–95. https://doi.org/10.5897/JHF2019.0588
• Theppakorn, T., Luthfiyyah, A., & Ploysri, K. (2014). Comparison of the Composition and Antioxidant Capacities of Green Teas Produced From the Assam and the Chinese Varieties Cultivated in Thailand. Journal of Microbiology, Biotechnology and Food Sciences, 3(5), 364–370. https://office2.jmbfs.org/index.php/JMBFS/article/view/6996
• Trinovani, E., Prawira-Atmaja, M. I., Kusmiyati, M., Harianto, S., Shabri, & Maulana, H. (2022). Total polyphenols and antioxidant activities of green tea powder from GMB 7 and GMB 9 tea clones. IOP Conference Series: Earth and Environmental Science, 974(1), 012113. https://doi.org/10.1088/1755-1315/974/1/012113
• Wachira, F.N., Kamunya, S., Karori, S., Chalo, R., & Maritim, T. (2013). The Tea Plants: botanical Aspects. In Tea in Health and Disease Prevention (pp. 3–17). Elsevier. https://doi.org/10.1016/B978-0-12-384937-3.00001-X
• Wang, H., Hua, J., Jiang, Y., Yang, Y., Wang, J., & Yuan, H. (2020). Influence of fixation methods on the chestnut-like aroma of green tea and dynamics of key aroma substances. Food Research International, 136, 109479. https://doi.org/10.1016/j.foodres.2020.109479
• Wang, L.-F., Park, S.-C., Chung, J.-O., Baik, J.-H., & Park, S.-K. (2004). The Compounds Contributing to the Greenness of Green Tea. Journal of Food Science, 69(8), S301–S305. https://doi.org/10.1111/j.1365-2621.2004.tb09894.x
• Wong, M., Sirisena, S., & Ng, K. (2022). Phytochemical profile of differently processed tea: A review. Journal of Food Science. https://doi.org/10.1111/1750-3841.16137
• Xu, J., Wang, M., Zhao, J., Wang, Y. H., Tang, Q., & Khan, I. A. (2018). Yellow tea (Camellia sinensis L.), a promising Chinese tea: Processing, chemical constituents and health benefits. In Food Research International (Vol. 107). https://doi.org/10.1016/j.foodres.2018.01.063
• Yadav, K., Parajuli, A., Khatri, B.B., & Shiwakoti, L.D. (2020). Phytochemicals and Quality of Green and Black Teas from Different Clones of Tea Plant. Journal of Food Quality, 2020, 1–13. https://doi.org/10.1155/2020/8874271
• Yang, J., & Liu, R.H. (2013). The phenolic profiles and antioxidant activity in different types of tea. International Journal of Food Science and Technology, 48(1), 163–171. https://doi.org/10.1111/j.1365-2621.2012.03173.x
• Yang, Z.-F., Bai, L.-P., Huang, W., Li, X.-Z., Zhao, S.-S., Zhong, N.-S., & Jiang, Z.-H. (2014). Comparison of in vitro antiviral activity of tea polyphenols against influenza A and B viruses and structure activity relationship analysis. Fitoterapia, 93, 47 53. https://doi.org/10.1016/j.fitote.2013.12.011
• Zhang, L., Ho, C., Zhou, J., Santos, J.S., Armstrong, L., & Granato, D. (2019). Chemistry and Biological Activities of Processed Camellia sinensis Teas: A Comprehensive Review. Comprehensive Reviews in Food Science and Food Safety, 18(5), 1474–1495. https://doi.org/10.1111/1541-4337.12479
• Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4), 555–559. https://doi.org/10.1016/S0308-8146(98)00102-2
• Zhu, N., Sang, S., Huang, T.-C., Bai, N., Yang, C.S., & Ho, C.-T. (2000). Antioxidant Chemistry of Green Tea Catechins: Oxidation Products of (-)-Epigallocatechin Gallate and (-)-Epigallocatechin with Peroxidase. Journal of Food Lipids, 7, 275–282.