Antioxidant activity, analgesic activity, and phytochemical analysis of Ammi majus (L.) extracts

Year 2023, Volume: 10 Issue: 1, 23 - 37, 26.03.2023

Otman El-guourramı Soufiane Drıoua Mouna Ameggouz Najoua Salhı Karima Sayah Gokhan Zengin , Ahmed Zahıdı , Anass Doukkalı Hanane Benzeıd

https://doi.org/10.21448/ijsm.1139246

Cited By: 3

Abstract

Ammi majus (L.) is commonly used to cure many diseases in Moroccan folk medicine, especially vitiligo. This research tries to evaluate the phytochemical constituents of two aqueous extracts (E1; Maceration; 48 h) and (E2: Infusion; 1h) and three organic fractions (F1: Cyclohexane), (F2: Ethyl acetate (EtOAc)) and (F3: Ethanolic (EtOH)) of A. majus (L.) seeds, as well as to study the antioxidant and analgesic activity of the species. Phytochemical analysis, antioxidant activity (DPPH, FRAP, ABTS, and TAC tests), and analgesic activity (writhing and tail immersion were induced by Acetic acid tests) were analyzed according to the literature. A quantitative phytochemical study indicate that the E1 had the highest content of total polyphenols (26.95 ± 0.53 mg GAE/g extract) and flavonoids (37.92 ± 0.46 mg QE/g extract), while F3 showed a promising flavonol content (24.26±0.08 mg QE/g extract). Tannins were found to be high in F1 (59.27 ± 0.16 mg CE/g extract) and F2 (57.65 ± 1.18 mg CE/g extract). Antioxidant results reveals that DPPH (IC50 = 179.68 ± 0.47 μg/mL) and FRAP (EC50 = 367.03 ± 0.12 μg/mL) show to E1 a high antioxidant activity. Regarding the analgesic activity of the different studied extracts, it was found that E1 has a high peripheral analgesic effect with 62.32 % and a high central analgesic potential throughout the experimentation at 500 mg/kg. Our studies demonstrated for the first time that A. majus seeds extracts have high antioxidant and analgesic activities through different analysis techniques.

Keywords

Ammi majus, Phytochemical analysis, Antioxidant, Analgesic

Thanks

It is a genuine pleasure to express my deep sense of thanks and gratitude to my guide professor Mohammed OULAD BOUYAHYA IDRISSI, and, all members of Laboratory of Analytical Chemistry and Bromatology of the Faculty of Medicine and Pharmacy in Rabat, Morocco.

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