Research Article
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Susceptibility of bacterial species isolated from mares to ozonated sunflower oil

Year 2023, Volume: 10 Issue: 1, 119 - 123, 26.03.2023
https://doi.org/10.21448/ijsm.1167867

Abstract

Sunflower oil is known for its therapeutic properties and culinary use. It is an important alimentary source of tocopherol and unsaturated fatty acids, and is used especially for wound healing. Studies on its antimicrobial potential, however, are lacking. The ozonation of oils of vegetable sources has been explored to enhance their therapeutic properties; however, studies that provide evidence of such benefits are still lacking. In the field of veterinary medicine, such data are even more scarce. In this study, the antimicrobial activity of ozonated sunflower oil was compared to that of non-ozonated oil, in an in vitro system, against strains of Staphylococcus aureus and Escherichia coli, isolated from intrauterine lavages of mares with endometritis. Tests were conducted using the minimum inhibitory concentration method. The ozonated oil was effective against S. aureus, whereas it was not against E. coli isolates. Our data open doors for discussion on the use of sunflower oil, with or without ozone treatment, for therapeutic purposes in veterinary medicine.

Supporting Institution

None

Project Number

None

Thanks

We are thankful to Lorena K. Alcântara, BS.Pharm, Sp.Pharm, for the relevant discussions on this work.

References

  • Ávila, A., Diniz, N.C., Serpa, R.T., Chaves, M., Viu, M., & de Oliveira, R.A. (2022). Effectiveness of Ozone Therapy in The Treatment of Endometritis in Mares. Journal of Equine Veterinary Science, 112, 103900. https://doi.org/10.1016/j.jevs.2022.103900
  • Clinical and Laboratory Standards Institute - CLSI. 2018. Performance standards for antimicrobial susceptibility testing; 27th informational supplement. M100-S28. Clinical and Laboratory Standards Institute, Wayne, PA.
  • Di Mauro, R., Cantarella, G., Bernardini, R., Di Rosa, M., Barbagallo, I., Distefano, A., Longhitano, L., Vicario, N., Nicolosi, D., Lazzarino, G., Tibullo, D., Gulino, M. E., Spampinato, M., Avola, R., & Li Volti, G. (2019). The Biochemical and Pharmacological Properties of Ozone: The Smell of Protection in Acute and Chronic Diseases. International Journal of Molecular Sciences, 20(3), 634. https://doi.org/10.3390/ijms20030634
  • Dias-Souza, M.V., Dos Santos, R.M., de Siqueira, E.P., & Ferreira-Marçal, P.H. (2017). Antibiofilm activity of cashew juice pulp against Staphylococcus aureus, high performance liquid chromatography/diode array detection and gas chromatography-mass spectrometry analyses, and interference on antimicrobial drugs. Journal of Food and Drug Analysis, 25(3), 589–596. https://doi.org/10.1016/j.jfda.2016.07.009
  • Dias-Souza, M.V., Dias, C.G., & Ferreira-Marçal, P.H. (2018). Interactions of natural products and antimicrobial drugs: Investigations of a dark matter in chemistry. Biointerface Research in Applied Chemistry, 8(3). 3259–3264
  • Filho, J. G.O., & Egea, M. B. (2021). Sunflower seed byproduct and its fractions for food application: An attempt to improve the sustainability of the oil process. Journal of Food Science, 86(5), 1497–1510. https://doi.org/10.1111/1750-3841.15719
  • Karasawa, T., & Steyger, P.S. (2011). Intracellular mechanisms of aminoglycoside-induced cytotoxicity. Integrative Biology: Quantitative Biosciences from Nano to Macro, 3(9), 879–886. https://doi.org/10.1039/c1ib00034a
  • Kassab, Z., El Achaby, M., Tamraoui, Y., Sehaqui, H., Bouhfid, R., & Qaiss, A. (2019). Sunflower oil cake-derived cellulose nanocrystals: Extraction, physico-chemical characteristics and potential application. International Journal of Biological Macromolecules, 136, 241–252. https://doi.org/10.1016/j.ijbiomac.2019.06.049
  • Krkl, C., Yiğit, M.V., Özercan, İ.H., Aygen, E., Gültürk, B., & Artaş, G. (2016). The Effect of Ozonated Olive Oil on Neovascularizatıon in an Experimental Skin Flap Model. Advances in Skin & Wound Care, 29(7), 322 327. https://doi.org/10.1097/01.ASW.0000484172.04260.46
  • Lai, X., Han, M.L., Ding, Y., Chow, S.H., Le Brun, A.P., Wu, C.M., Bergen, P.J., Jiang, J.H., Hsu, H.Y., Muir, B.W., White, J., Song, J., Li, J., & Shen, H.H. (2022). A polytherapy based approach to combat antimicrobial resistance using cubosomes. Nature Communications, 13(1), 343. https://doi.org/10.1038/s41467-022-28012-5
  • Poljšak, N., Kreft, S., & Kočevar Glavač, N. (2020). Vegetable butters and oils in skin wound healing: Scientific evidence for new opportunities in dermatology. Phytotherapy Research: PTR, 34(2), 254–269. https://doi.org/10.1002/ptr.6524
  • Rai, A., Mohanty, B., & Bhargava, R. (2016). Supercritical extraction of sunflower oil: A central composite design for extraction variables. Food Chemistry, 192, 647–659. https://doi.org/10.1016/j.foodchem.2015.07.070
  • Rauf, S., Jamil, N., Tariq, S.A., Khan, M., Kausar, M., & Kaya, Y. (2017). Progress in modification of sunflower oil to expand its industrial value. Journal of the Science of Food and Agriculture, 97(7), 1997–2006. https://doi.org/10.1002/jsfa.8214
  • Schwarz, S., Loeffler, A., & Kadlec, K. (2017). Bacterial resistance to antimicrobial agents and its impact on veterinary and human medicine. Veterinary Dermatology, 28(1), 82–e19. https://doi.org/10.1111/vde.12362
  • Sechi, L.A., Lezcano, I., Nunez, N., Espim, M., Duprè, I., Pinna, A., Molicotti, P., Fadda, G., & Zanetti, S. (2001). Antibacterial activity of ozonized sunflower oil (Oleozon). Journal of Applied Microbiology, 90(2), 279–284. https://doi.org/10.1046/j.1365-2672.2001.01235.x
  • Tabassum N., & Vidyasagar, G. M. (2014). In-vitro antimicrobial activity of edible oils against human pathogens causing skin infections. International Journal of Pharmaceutical Sciences and Research, 5(10), 4493-4498. https://doi.org/10.13040/IJPSR.0975-8232.5(10).4493-98
  • Weis, C., Cuénod, A., Rieck, B., Dubuis, O., Graf, S., Lang, C., Oberle, M., Brackmann, M., Søgaard, K.K., Osthoff, M., Borgwardt, K., & Egli, A. (2022). Direct antimicrobial resistance prediction from clinical MALDI-TOF mass spectra using machine learning. Nature Medicine, 28(1), 164–174. https://doi.org/10.1038/s41591-021-01619-9

Susceptibility of bacterial species isolated from mares to ozonated sunflower oil

Year 2023, Volume: 10 Issue: 1, 119 - 123, 26.03.2023
https://doi.org/10.21448/ijsm.1167867

Abstract

Sunflower oil is known for its therapeutic properties and culinary use. It is an important alimentary source of tocopherol and unsaturated fatty acids, and is used especially for wound healing. Studies on its antimicrobial potential, however, are lacking. The ozonation of oils of vegetable sources has been explored to enhance their therapeutic properties; however, studies that provide evidence of such benefits are still lacking. In the field of veterinary medicine, such data are even more scarce. In this study, the antimicrobial activity of ozonated sunflower oil was compared to that of non-ozonated oil, in an in vitro system, against strains of Staphylococcus aureus and Escherichia coli, isolated from intrauterine lavages of mares with endometritis. Tests were conducted using the minimum inhibitory concentration method. The ozonated oil was effective against S. aureus, whereas it was not against E. coli isolates. Our data open doors for discussion on the use of sunflower oil, with or without ozone treatment, for therapeutic purposes in veterinary medicine.

Project Number

None

References

  • Ávila, A., Diniz, N.C., Serpa, R.T., Chaves, M., Viu, M., & de Oliveira, R.A. (2022). Effectiveness of Ozone Therapy in The Treatment of Endometritis in Mares. Journal of Equine Veterinary Science, 112, 103900. https://doi.org/10.1016/j.jevs.2022.103900
  • Clinical and Laboratory Standards Institute - CLSI. 2018. Performance standards for antimicrobial susceptibility testing; 27th informational supplement. M100-S28. Clinical and Laboratory Standards Institute, Wayne, PA.
  • Di Mauro, R., Cantarella, G., Bernardini, R., Di Rosa, M., Barbagallo, I., Distefano, A., Longhitano, L., Vicario, N., Nicolosi, D., Lazzarino, G., Tibullo, D., Gulino, M. E., Spampinato, M., Avola, R., & Li Volti, G. (2019). The Biochemical and Pharmacological Properties of Ozone: The Smell of Protection in Acute and Chronic Diseases. International Journal of Molecular Sciences, 20(3), 634. https://doi.org/10.3390/ijms20030634
  • Dias-Souza, M.V., Dos Santos, R.M., de Siqueira, E.P., & Ferreira-Marçal, P.H. (2017). Antibiofilm activity of cashew juice pulp against Staphylococcus aureus, high performance liquid chromatography/diode array detection and gas chromatography-mass spectrometry analyses, and interference on antimicrobial drugs. Journal of Food and Drug Analysis, 25(3), 589–596. https://doi.org/10.1016/j.jfda.2016.07.009
  • Dias-Souza, M.V., Dias, C.G., & Ferreira-Marçal, P.H. (2018). Interactions of natural products and antimicrobial drugs: Investigations of a dark matter in chemistry. Biointerface Research in Applied Chemistry, 8(3). 3259–3264
  • Filho, J. G.O., & Egea, M. B. (2021). Sunflower seed byproduct and its fractions for food application: An attempt to improve the sustainability of the oil process. Journal of Food Science, 86(5), 1497–1510. https://doi.org/10.1111/1750-3841.15719
  • Karasawa, T., & Steyger, P.S. (2011). Intracellular mechanisms of aminoglycoside-induced cytotoxicity. Integrative Biology: Quantitative Biosciences from Nano to Macro, 3(9), 879–886. https://doi.org/10.1039/c1ib00034a
  • Kassab, Z., El Achaby, M., Tamraoui, Y., Sehaqui, H., Bouhfid, R., & Qaiss, A. (2019). Sunflower oil cake-derived cellulose nanocrystals: Extraction, physico-chemical characteristics and potential application. International Journal of Biological Macromolecules, 136, 241–252. https://doi.org/10.1016/j.ijbiomac.2019.06.049
  • Krkl, C., Yiğit, M.V., Özercan, İ.H., Aygen, E., Gültürk, B., & Artaş, G. (2016). The Effect of Ozonated Olive Oil on Neovascularizatıon in an Experimental Skin Flap Model. Advances in Skin & Wound Care, 29(7), 322 327. https://doi.org/10.1097/01.ASW.0000484172.04260.46
  • Lai, X., Han, M.L., Ding, Y., Chow, S.H., Le Brun, A.P., Wu, C.M., Bergen, P.J., Jiang, J.H., Hsu, H.Y., Muir, B.W., White, J., Song, J., Li, J., & Shen, H.H. (2022). A polytherapy based approach to combat antimicrobial resistance using cubosomes. Nature Communications, 13(1), 343. https://doi.org/10.1038/s41467-022-28012-5
  • Poljšak, N., Kreft, S., & Kočevar Glavač, N. (2020). Vegetable butters and oils in skin wound healing: Scientific evidence for new opportunities in dermatology. Phytotherapy Research: PTR, 34(2), 254–269. https://doi.org/10.1002/ptr.6524
  • Rai, A., Mohanty, B., & Bhargava, R. (2016). Supercritical extraction of sunflower oil: A central composite design for extraction variables. Food Chemistry, 192, 647–659. https://doi.org/10.1016/j.foodchem.2015.07.070
  • Rauf, S., Jamil, N., Tariq, S.A., Khan, M., Kausar, M., & Kaya, Y. (2017). Progress in modification of sunflower oil to expand its industrial value. Journal of the Science of Food and Agriculture, 97(7), 1997–2006. https://doi.org/10.1002/jsfa.8214
  • Schwarz, S., Loeffler, A., & Kadlec, K. (2017). Bacterial resistance to antimicrobial agents and its impact on veterinary and human medicine. Veterinary Dermatology, 28(1), 82–e19. https://doi.org/10.1111/vde.12362
  • Sechi, L.A., Lezcano, I., Nunez, N., Espim, M., Duprè, I., Pinna, A., Molicotti, P., Fadda, G., & Zanetti, S. (2001). Antibacterial activity of ozonized sunflower oil (Oleozon). Journal of Applied Microbiology, 90(2), 279–284. https://doi.org/10.1046/j.1365-2672.2001.01235.x
  • Tabassum N., & Vidyasagar, G. M. (2014). In-vitro antimicrobial activity of edible oils against human pathogens causing skin infections. International Journal of Pharmaceutical Sciences and Research, 5(10), 4493-4498. https://doi.org/10.13040/IJPSR.0975-8232.5(10).4493-98
  • Weis, C., Cuénod, A., Rieck, B., Dubuis, O., Graf, S., Lang, C., Oberle, M., Brackmann, M., Søgaard, K.K., Osthoff, M., Borgwardt, K., & Egli, A. (2022). Direct antimicrobial resistance prediction from clinical MALDI-TOF mass spectra using machine learning. Nature Medicine, 28(1), 164–174. https://doi.org/10.1038/s41591-021-01619-9
There are 17 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences
Journal Section Articles
Authors

Gabriel Souza Dos Santos This is me 0000-0002-2801-0214

Arthur Azevedo Perpétuo This is me 0000-0002-3863-8718

Marcus Vinícius Dias Souza 0000-0001-5723-5095

Project Number None
Publication Date March 26, 2023
Submission Date August 28, 2022
Published in Issue Year 2023 Volume: 10 Issue: 1

Cite

APA Dos Santos, G. S., Perpétuo, A. A., & Dias Souza, M. V. (2023). Susceptibility of bacterial species isolated from mares to ozonated sunflower oil. International Journal of Secondary Metabolite, 10(1), 119-123. https://doi.org/10.21448/ijsm.1167867
International Journal of Secondary Metabolite

e-ISSN: 2148-6905