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Effect of solvent polarity of phytochemical analysis of different Moroccan sites of Opuntia ficus-indica (L.) Mill. pads

Year 2024, Volume: 11 Issue: 1, 93 - 104, 05.02.2024
https://doi.org/10.21448/ijsm.1354740

Abstract

The current study focuses on the study of phenolic compounds in methanol, dichloromethane, and chloroform extracts as solvents with increasing polarity, in order to quantify the secondary metabolites of Opuntia ficus indica pads collected from three Moroccan sites (Oulad Boubker, Imzouren, and Skoura). The findings showthat betalains and carotenoids were present, in varying amounts depending on the study area. The methanolic extracts had the concentrations of compounds. When analyzing the chemical composition using Gas Chromatography-Mass Spectrometry, it was found that linoleic acid was the unsaturated fatty acid accounting for 14.279%. Palmitic acid, fatty acid derivatives and alkanes were also detected in all regions. Among the extracts Imzouren had the concentration of fatty acids (24.874%) followed by Oulad Boubker (13.907%) and Skoura (13.319%). However, oleic acid was detected only in the extract of Skoura. Hence, we were able to confirm that the chemical differences among the various Opuntia extracts were primarily influenced by the selection of the extracting solvent and the prevailing climatic and geographical factors.

References

  • Alsaad, A.J.A., Altemimi, A.B., Aziz, S.N., & Lakhssassi, N. (2019). Extraction and identification of cactus Opuntia dillenii seed oil and its added value for human health benefits. Pharmacognosy Journal, 11(3), 579–587. https://doi.org/10.5530/pj.2019.11.92
  • Amorim-Carrilho, K.T., Cepeda, A., Fente, C., & Regal, P. (2014). Review of methods for analysis of carotenoids. Trends in Analytical Chemistry, 56, 49 73. https://doi.org/10.1016/j.trac.2013.12.011
  • Bensadón, S., Hervert-Hernández, D., Sáyago-Ayerdi, S. G., & Goñi, I. (2010). By-products of Opuntia ficus-indica as a source of antioxidant dietary fiber. Plant foods for human nutrition (Dordrecht, Netherlands), 65(3), 210–216. https://doi.org/10.1007/s11130-010-0176-2
  • Boutakiout, A. (2015). Etude physico-chimique, biochimique et stabilité d’un nouveau produit: jus de cladode du figuier de Barbarie marocain (Opuntia ficus-indica et Opuntia megacantha). Doctoral thesis, Agronomie, Université Sultan Moulay Slimane, Morocco.
  • Bouzoubaâ, Z., Essoukrati, Y., Tahrouch, S., Hatimi, A., Gharby, S., & Harhar, H. (2014). Etude physico-chimique de deux variétés de figuier de barbarie ('Achefri'et'Amouslem') du Sud marocain. Les technologies de laboratoire, 8(34).
  • Cai, Y., Sun, M., Wu, H., Huang, R., & Corke, H. (1998). Characterization and Quantification of Betacyanin Pigments from Diverse Amaranthus Species. Journal of Agricultural and Food Chemistry, 46(6), 2063-2070. https://doi.org/10.1021/jf9709966
  • Castellanos-Santiago, E., & Yahia, E.M. (2008). Identification and quantification of betalains from the fruits of 10 mexican prickly pear cultivars by high-performance liquid chromatography and electrospray ionization mass spectrometry. Journal of agricultural and food chemistry, 56(14), 5758–5764. https://doi.org/10.1021/jf800362t
  • De Santiago, E., Domínguez-Fernández, M., Cid, C., & De Peña, M.P. (2018). Impact of cooking process on nutritional composition and antioxidants of cactus cladodes (Opuntia ficus indica). Food Chemistry, 240, 1055 1062. https://doi.org/10.1016/j.foodchem.2017.08.039
  • De Wit, M., du Toit, A., Osthoff, G., & Hugo, A. (2019). Cactus pear antioxidants: a comparison between fruit pulp, fruit peel, fruit seeds and cladodes of eight different cactus pear cultivars (Opuntia ficus-indica and Opuntia robusta). Food Measure, 13, 2347–2356. https://doi.org/10.1007/s11694-019-00154-z
  • Dias, M.G., Camões, M.F.G., & Oliveira, L. (2009). Carotenoids in traditional Portuguese fruits and vegetables. Food Chemistry, 113(3), 808 815. https://doi.org/10.1016/j.foodchem.2008.08.002
  • Djeridane, A., Yousfi, M., Nadjemi, B., Vidal, N., Lesgards, J.F., & Stocker, P. (2007). Screening of some Algerian medicinal plants for the phenolic compounds and their antioxidant activity. European Food Research and Technology, 224, 801 809. https://doi.org/10.1007/s00217-006-0361-6
  • Gallegos-Infante, J.A., Rocha-Guzman, N.E., González-Laredo, R.F., Reynoso-Camacho, R., Medina-Torres, L., & Cervantes-Cardozo, V. (2009). Effect of air flow rate on the polyphenols content and antioxidant capacity of convective dried cactus pear cladodes (Opuntia ficus indica). International journal of food sciences and nutrition, 60(sup2), 80-87. https://doi.org/10.1080/09637480802477691
  • Guevara-Figueroa, T., Jiménez-Islas, H., Reyes-Escogido, M.D., Mortensen, A.G., Laursen, B.B., Lin, L., Léon-Rodríguez, A.D., Fomsgaard, I.S., & Rosa, A.P. (2010). Proximate composition, phenolic acids, and flavonoids characterization of commercial and wild nopal (Opuntia spp.). Journal of Food Composition and Analysis, 23, 525 532. https://doi.org/10.1016/j.jfca.2009.12.003
  • Hernández-Urbiola, M.I., Pérez-Torrero, E., & Rodríguez-García, M.E. (2011). Chemical analysis of nutritional content of prickly pads (Opuntia ficus indica) at varied ages in an organic harvest. International journal of environmental research and public health, 8(5), 1287–1295. https://doi.org/10.3390/ijerph8051287
  • Inglese, P., Saenz, C., Mondragon, C., Nefzaoui, A., Louhaichi, M. (2018). Ecologie, culture et utilisations du figuier de barbarie. Rome, Italy: FAO.
  • ISO (Ed.). (2003). Technical Specification. Crocus sativus L. Saffron. Ed, ISO, Geneva, Switzerland. TS 3632–1/2.
  • Jaramillo-Flores, M.E., González-Cruz, L., Cornejo-Mazon, M., Dorantes-Alvarez, L., Gutierrez-Lopez, G.F., & Hernandez-Sanchez, H. (2003). Effect of thermal treatment on the antioxidant activity and content of carotenoids and phenolic compounds of cactus pear pads (Opuntia ficus-indica). Food science and technology international, 9(4), 271-278. https://doi.org/10.1177/108201303036093
  • Kosalec, I., Pepeljnjak, S., Bakmaz, M., & Vladimir-Knežević, S.A.N.D.A. (2005). Flavonoid analysis and antimicrobial activity of commercially available propolis products. Acta Pharmaceutica, 55(4), 423-430.
  • Lahlou, F.A., Hmimid, F., Tahiri Jouti, N., Bellali, F., Loutfi, M., & Bourhim, N. (2014). Phytochemical screening chemical composition and toxicity of Euphorbia echinus. Journal of Biological and Chemical Research, 31, 21-38.
  • Liu, W., Fu, Y.J., Zu, Y.G., Tong, M.H., Wu, N., Liu, X.L., & Zhang, S. (2009). Supercritical carbon dioxide extraction of seed oil from Opuntia dillenii Haw. and its antioxidant activity. Food Chemistry, 114(1), 334-339. https://doi.org/10.1016/j.foodchem.2008.09.049
  • Loukili, E. H., Abrigach, F., Bouhrim, M., Bnouham, M., Fauconnier, M. L., & Ramdani, M. (2021). Chemical composition and physicochemical analysis of Opuntia dillenii extracts grown in Morocco. Journal of Chemistry, 2021, 1 11. https://doi.org/10.1155/2021/8858929
  • Marangoni, F., Agostoni, C., Borghi, C., Catapano, A.L., Cena, H., Ghiselli, A., ... & Poli, A. (2020). Dietary linoleic acid and human health: Focus on cardiovascular and cardiometabolic effects. Atherosclerosis, 292, 90 98. https://doi.org/10.1016/j.atherosclerosis.2019.11.018
  • Mena, P., Tassotti, M., Andreu, L., Nuncio-Jáuregui, N., Legua, P., Del Rio, D., & Hernández, F. (2018). Phytochemical characterization of different prickly pear (Opuntia ficus-indica (L.) Mill.) cultivars and botanical parts: UHPLC-ESI-MSn metabolomics profiles and their chemometric analysis. Food Research International, 108, 301 308. https://doi.org/10.1016/j.foodres.2018.03.062
  • Mouas, N.T., Kabouche, Z., Bellel, N., & Chertout, L.K. (2021). Opuntia ficus-indica a Mediterranean Diet Product. Proceedings of the 1st International Electronic Conference on Biological Diversity, Ecology and Evolution, online, 15 31 March, 68. https://doi.org/10.3390/BDEE2021-09403
  • Moussaoui. B. (2020). Propriétés biologiques d’extraits des pads d'Opuntia Ficus Indica (L). Doctoral thesis, Universite Abdelhamid Ibn Badis Mostaganem, Algérie.
  • Msaddak. L. (2018). Propriétés techno-fonctionnelles et substances bioactives de deux ingrédients alimentaires pads du figuier de barbarie et feuilles de vigne [Unpublished Doctoral thesis]. Université de Gabès, Tunisie.
  • Piga, A. (2004). Cactus pear: a fruit of nutraceutical and functional importance. Journal of the professional association for Cactus development, 6, 9-22.
  • Sanchez-Gonzalez, N., Jaime-Fonseca, M.R., San Martin-Martinez, E., & Zepeda, L.G. (2013). Extraction, stability, and separation of betalains from Opuntia joconostle cv. using response surface methodology. Journal of agricultural and food chemistry, 61(49), 11995–12004. https://doi.org/10.1021/jf401705h
  • Sass-Kiss, A., Kiss, J., Milotay, P., Kerek, M.M., & Toth-Markus, M. (2005). Differences in anthocyanin and carotenoid content of fruits and vegetables. Food Research International, 38(8–9), 1023-1029. https://doi.org/10.1016/j.foodres.2005.03.014
  • Serra, A.T., Poejo, J., Matias, A.A., Bronze, M.R., & Duarte, C.M. (2013). Evaluation of Opuntia spp. derived products as antiproliferative agents in human colon cancer cell line (HT29). Food Research International, 54(1), 892 901. https://doi.org/10.1016/j.foodres.2013.08.043
  • Singleton, V.L., Orthofer, R., & Lamuela-Raventós, R.M. (1999). [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In Methods in Enzymology, (299), 152-178. Academic Press. https://doi.org/10.1016/S0076-6879(99)99017-1
  • Tsao, R., & Deng, Z. (2004). Separation procedures for naturally occurring antioxidant phytochemicals. Journal of Chromatography, B, Analytical Technologies in the Biomedical and Life Sciences, 812(1-2), 85–99. https://doi.org/10.1016/j.jchromb.2004.09.028
  • Amjad Khan, W., Chun-Mei, H., Khan, N., Iqbal, A., Lyu, S.W., & Shah, F. (2017). Bioengineered Plants Can Be a Useful Source of Omega-3 Fatty Acids. BioMed research international, 2017, 7348919. https://doi.org/10.1155/2017/7348919
  • Willis, R. (1998). Improved method for measuring hydrolyzable tannins using potassium iodate. Analyst, 123(3), 435-439. https://doi.org/10.1039/A706862J

Effect of solvent polarity of phytochemical analysis of different Moroccan sites of Opuntia ficus-indica (L.) Mill. pads

Year 2024, Volume: 11 Issue: 1, 93 - 104, 05.02.2024
https://doi.org/10.21448/ijsm.1354740

Abstract

The current study focuses on the study of phenolic compounds in methanol, dichloromethane, and chloroform extracts as solvents with increasing polarity, in order to quantify the secondary metabolites of Opuntia ficus indica pads collected from three Moroccan sites (Oulad Boubker, Imzouren, and Skoura). The findings showthat betalains and carotenoids were present, in varying amounts depending on the study area. The methanolic extracts had the concentrations of compounds. When analyzing the chemical composition using Gas Chromatography-Mass Spectrometry, it was found that linoleic acid was the unsaturated fatty acid accounting for 14.279%. Palmitic acid, fatty acid derivatives and alkanes were also detected in all regions. Among the extracts Imzouren had the concentration of fatty acids (24.874%) followed by Oulad Boubker (13.907%) and Skoura (13.319%). However, oleic acid was detected only in the extract of Skoura. Hence, we were able to confirm that the chemical differences among the various Opuntia extracts were primarily influenced by the selection of the extracting solvent and the prevailing climatic and geographical factors.

References

  • Alsaad, A.J.A., Altemimi, A.B., Aziz, S.N., & Lakhssassi, N. (2019). Extraction and identification of cactus Opuntia dillenii seed oil and its added value for human health benefits. Pharmacognosy Journal, 11(3), 579–587. https://doi.org/10.5530/pj.2019.11.92
  • Amorim-Carrilho, K.T., Cepeda, A., Fente, C., & Regal, P. (2014). Review of methods for analysis of carotenoids. Trends in Analytical Chemistry, 56, 49 73. https://doi.org/10.1016/j.trac.2013.12.011
  • Bensadón, S., Hervert-Hernández, D., Sáyago-Ayerdi, S. G., & Goñi, I. (2010). By-products of Opuntia ficus-indica as a source of antioxidant dietary fiber. Plant foods for human nutrition (Dordrecht, Netherlands), 65(3), 210–216. https://doi.org/10.1007/s11130-010-0176-2
  • Boutakiout, A. (2015). Etude physico-chimique, biochimique et stabilité d’un nouveau produit: jus de cladode du figuier de Barbarie marocain (Opuntia ficus-indica et Opuntia megacantha). Doctoral thesis, Agronomie, Université Sultan Moulay Slimane, Morocco.
  • Bouzoubaâ, Z., Essoukrati, Y., Tahrouch, S., Hatimi, A., Gharby, S., & Harhar, H. (2014). Etude physico-chimique de deux variétés de figuier de barbarie ('Achefri'et'Amouslem') du Sud marocain. Les technologies de laboratoire, 8(34).
  • Cai, Y., Sun, M., Wu, H., Huang, R., & Corke, H. (1998). Characterization and Quantification of Betacyanin Pigments from Diverse Amaranthus Species. Journal of Agricultural and Food Chemistry, 46(6), 2063-2070. https://doi.org/10.1021/jf9709966
  • Castellanos-Santiago, E., & Yahia, E.M. (2008). Identification and quantification of betalains from the fruits of 10 mexican prickly pear cultivars by high-performance liquid chromatography and electrospray ionization mass spectrometry. Journal of agricultural and food chemistry, 56(14), 5758–5764. https://doi.org/10.1021/jf800362t
  • De Santiago, E., Domínguez-Fernández, M., Cid, C., & De Peña, M.P. (2018). Impact of cooking process on nutritional composition and antioxidants of cactus cladodes (Opuntia ficus indica). Food Chemistry, 240, 1055 1062. https://doi.org/10.1016/j.foodchem.2017.08.039
  • De Wit, M., du Toit, A., Osthoff, G., & Hugo, A. (2019). Cactus pear antioxidants: a comparison between fruit pulp, fruit peel, fruit seeds and cladodes of eight different cactus pear cultivars (Opuntia ficus-indica and Opuntia robusta). Food Measure, 13, 2347–2356. https://doi.org/10.1007/s11694-019-00154-z
  • Dias, M.G., Camões, M.F.G., & Oliveira, L. (2009). Carotenoids in traditional Portuguese fruits and vegetables. Food Chemistry, 113(3), 808 815. https://doi.org/10.1016/j.foodchem.2008.08.002
  • Djeridane, A., Yousfi, M., Nadjemi, B., Vidal, N., Lesgards, J.F., & Stocker, P. (2007). Screening of some Algerian medicinal plants for the phenolic compounds and their antioxidant activity. European Food Research and Technology, 224, 801 809. https://doi.org/10.1007/s00217-006-0361-6
  • Gallegos-Infante, J.A., Rocha-Guzman, N.E., González-Laredo, R.F., Reynoso-Camacho, R., Medina-Torres, L., & Cervantes-Cardozo, V. (2009). Effect of air flow rate on the polyphenols content and antioxidant capacity of convective dried cactus pear cladodes (Opuntia ficus indica). International journal of food sciences and nutrition, 60(sup2), 80-87. https://doi.org/10.1080/09637480802477691
  • Guevara-Figueroa, T., Jiménez-Islas, H., Reyes-Escogido, M.D., Mortensen, A.G., Laursen, B.B., Lin, L., Léon-Rodríguez, A.D., Fomsgaard, I.S., & Rosa, A.P. (2010). Proximate composition, phenolic acids, and flavonoids characterization of commercial and wild nopal (Opuntia spp.). Journal of Food Composition and Analysis, 23, 525 532. https://doi.org/10.1016/j.jfca.2009.12.003
  • Hernández-Urbiola, M.I., Pérez-Torrero, E., & Rodríguez-García, M.E. (2011). Chemical analysis of nutritional content of prickly pads (Opuntia ficus indica) at varied ages in an organic harvest. International journal of environmental research and public health, 8(5), 1287–1295. https://doi.org/10.3390/ijerph8051287
  • Inglese, P., Saenz, C., Mondragon, C., Nefzaoui, A., Louhaichi, M. (2018). Ecologie, culture et utilisations du figuier de barbarie. Rome, Italy: FAO.
  • ISO (Ed.). (2003). Technical Specification. Crocus sativus L. Saffron. Ed, ISO, Geneva, Switzerland. TS 3632–1/2.
  • Jaramillo-Flores, M.E., González-Cruz, L., Cornejo-Mazon, M., Dorantes-Alvarez, L., Gutierrez-Lopez, G.F., & Hernandez-Sanchez, H. (2003). Effect of thermal treatment on the antioxidant activity and content of carotenoids and phenolic compounds of cactus pear pads (Opuntia ficus-indica). Food science and technology international, 9(4), 271-278. https://doi.org/10.1177/108201303036093
  • Kosalec, I., Pepeljnjak, S., Bakmaz, M., & Vladimir-Knežević, S.A.N.D.A. (2005). Flavonoid analysis and antimicrobial activity of commercially available propolis products. Acta Pharmaceutica, 55(4), 423-430.
  • Lahlou, F.A., Hmimid, F., Tahiri Jouti, N., Bellali, F., Loutfi, M., & Bourhim, N. (2014). Phytochemical screening chemical composition and toxicity of Euphorbia echinus. Journal of Biological and Chemical Research, 31, 21-38.
  • Liu, W., Fu, Y.J., Zu, Y.G., Tong, M.H., Wu, N., Liu, X.L., & Zhang, S. (2009). Supercritical carbon dioxide extraction of seed oil from Opuntia dillenii Haw. and its antioxidant activity. Food Chemistry, 114(1), 334-339. https://doi.org/10.1016/j.foodchem.2008.09.049
  • Loukili, E. H., Abrigach, F., Bouhrim, M., Bnouham, M., Fauconnier, M. L., & Ramdani, M. (2021). Chemical composition and physicochemical analysis of Opuntia dillenii extracts grown in Morocco. Journal of Chemistry, 2021, 1 11. https://doi.org/10.1155/2021/8858929
  • Marangoni, F., Agostoni, C., Borghi, C., Catapano, A.L., Cena, H., Ghiselli, A., ... & Poli, A. (2020). Dietary linoleic acid and human health: Focus on cardiovascular and cardiometabolic effects. Atherosclerosis, 292, 90 98. https://doi.org/10.1016/j.atherosclerosis.2019.11.018
  • Mena, P., Tassotti, M., Andreu, L., Nuncio-Jáuregui, N., Legua, P., Del Rio, D., & Hernández, F. (2018). Phytochemical characterization of different prickly pear (Opuntia ficus-indica (L.) Mill.) cultivars and botanical parts: UHPLC-ESI-MSn metabolomics profiles and their chemometric analysis. Food Research International, 108, 301 308. https://doi.org/10.1016/j.foodres.2018.03.062
  • Mouas, N.T., Kabouche, Z., Bellel, N., & Chertout, L.K. (2021). Opuntia ficus-indica a Mediterranean Diet Product. Proceedings of the 1st International Electronic Conference on Biological Diversity, Ecology and Evolution, online, 15 31 March, 68. https://doi.org/10.3390/BDEE2021-09403
  • Moussaoui. B. (2020). Propriétés biologiques d’extraits des pads d'Opuntia Ficus Indica (L). Doctoral thesis, Universite Abdelhamid Ibn Badis Mostaganem, Algérie.
  • Msaddak. L. (2018). Propriétés techno-fonctionnelles et substances bioactives de deux ingrédients alimentaires pads du figuier de barbarie et feuilles de vigne [Unpublished Doctoral thesis]. Université de Gabès, Tunisie.
  • Piga, A. (2004). Cactus pear: a fruit of nutraceutical and functional importance. Journal of the professional association for Cactus development, 6, 9-22.
  • Sanchez-Gonzalez, N., Jaime-Fonseca, M.R., San Martin-Martinez, E., & Zepeda, L.G. (2013). Extraction, stability, and separation of betalains from Opuntia joconostle cv. using response surface methodology. Journal of agricultural and food chemistry, 61(49), 11995–12004. https://doi.org/10.1021/jf401705h
  • Sass-Kiss, A., Kiss, J., Milotay, P., Kerek, M.M., & Toth-Markus, M. (2005). Differences in anthocyanin and carotenoid content of fruits and vegetables. Food Research International, 38(8–9), 1023-1029. https://doi.org/10.1016/j.foodres.2005.03.014
  • Serra, A.T., Poejo, J., Matias, A.A., Bronze, M.R., & Duarte, C.M. (2013). Evaluation of Opuntia spp. derived products as antiproliferative agents in human colon cancer cell line (HT29). Food Research International, 54(1), 892 901. https://doi.org/10.1016/j.foodres.2013.08.043
  • Singleton, V.L., Orthofer, R., & Lamuela-Raventós, R.M. (1999). [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In Methods in Enzymology, (299), 152-178. Academic Press. https://doi.org/10.1016/S0076-6879(99)99017-1
  • Tsao, R., & Deng, Z. (2004). Separation procedures for naturally occurring antioxidant phytochemicals. Journal of Chromatography, B, Analytical Technologies in the Biomedical and Life Sciences, 812(1-2), 85–99. https://doi.org/10.1016/j.jchromb.2004.09.028
  • Amjad Khan, W., Chun-Mei, H., Khan, N., Iqbal, A., Lyu, S.W., & Shah, F. (2017). Bioengineered Plants Can Be a Useful Source of Omega-3 Fatty Acids. BioMed research international, 2017, 7348919. https://doi.org/10.1155/2017/7348919
  • Willis, R. (1998). Improved method for measuring hydrolyzable tannins using potassium iodate. Analyst, 123(3), 435-439. https://doi.org/10.1039/A706862J
There are 34 citations in total.

Details

Primary Language English
Subjects Plant Biochemistry
Journal Section Articles
Authors

Sara Razzak 0000-0002-5141-6052

Marouane Aoujı This is me 0000-0002-5884-0016

Chaima Sabrı This is me 0009-0003-1540-645X

Hiba Benchehıda This is me 0009-0002-6174-2879

Mariame Taıbı 0000-0003-2137-8566

Youness Taboz This is me 0000-0003-2485-9592

Publication Date February 5, 2024
Submission Date September 7, 2023
Published in Issue Year 2024 Volume: 11 Issue: 1

Cite

APA Razzak, S., Aoujı, M., Sabrı, C., Benchehıda, H., et al. (2024). Effect of solvent polarity of phytochemical analysis of different Moroccan sites of Opuntia ficus-indica (L.) Mill. pads. International Journal of Secondary Metabolite, 11(1), 93-104. https://doi.org/10.21448/ijsm.1354740
International Journal of Secondary Metabolite

e-ISSN: 2148-6905