Research Article
BibTex RIS Cite

In vitro antimicrobial and antioxidant activities of Sambucus williamsii and Sambucus pendula

Year 2024, Volume: 11 Issue: 2, 191 - 199, 03.06.2024
https://doi.org/10.21448/ijsm.1353669

Abstract

The present study aimed to compare the in vitro antimicrobial and antioxidant activities of Sambucus williamsii and Sambucus pendula. The antimicrobial activity of the two plants was evaluated using the disc diffusion and minimal inhibition concentration (MIC) method against three Gram-positive bacteria (Bacillus cereus ATCC 11778, Bacillus subtilis ATCC 9327 and Listeria monocytogenes ATCC 15313), four Gram-negative bacteria (Escherichia coli ATCC 15489, Psendomonas aeruginosa ATCC 27853, Pseudomonas fluorescens KCCM 41443 and Salmonella typhimurium KCCM 11862) and one yeast (Saccharomyces cerevisiae IFO 1950). The tested ether and ethyl acetate fractions of ethanol extract from Sambucus williamsii showed significant antimicrobial activity against Bacillus cereus and Pseudomonas fluorescens; conversely, the ether fraction of Sambucus pendula leaf showed no clear zone formation against any tested bacteria. In general, the fractions of the two Sambucus species exhibited a lower MIC against tested Gram-negative bacteria than the tested Gram-positive bacteria. The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay. The results showed that the half inhibitory concentration (IC50) for the ethanol extract of Sambucus williamsii was the lower value, which means the greater antioxidant activity. On the other hand, the IC50 value of the hot water extract of Sambucus pendula was the lower value. The richness of the total polyphenol contents of the two Sambucus implies their potential as raw material sources for the pharmaceutical and cosmeceutical industries.

Supporting Institution

Sunchon National University

References

  • Akhtar, N., Ihsan-ul-Haq, & Mirza, B. (2018). Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species Arabian Journal of Chemistry, 11(8), 1223-1235. https://doi.org/10.1016/j.arabjc.2015.01.013
  • Barak, T.H., Celep, E., İnan, Y., & Yeşilada, E. (2020) In vitro human digestion simulation of the bioavailability and antioxidant activity of phenolics from Sambucus ebulus L. fruit extracts. Food Bioscience, 37, 100711. https://doi.org/10.1016/j.fbio.2020.100711
  • Blois, M.S. (1958). Antioxidant determination by the use of a stable free radical. Nature, 26, 1199-1202.
  • Chae, J.W., & Cho, Y.J. (2012). Antioxidant activity of extracts from Sambucus williamsii var. coreana. Korean Journal of Plant Resources, 25(4), 363-371.
  • Ding, H.-Y., Chou, T.-H., Lin, R.-J., Chan, L.-P., Wang, G.-H., & Liang, C.-H. (2011). Antioxidant and antimelanogenic behaviors of Paeonia suffruticosa. Plant Foods for Human Nutrition, 66, 275–284. https://doi.org/10.1007/s11130-011-0235-3.
  • Dulf, F.V., Vodnar, D.C., Dulf, E.-H., & Toşa, M.I. (2015). Total Phenolic Contents, Antioxidant Activities, and Lipid Fractions from Berry Pomaces Obtained by Solid-State Fermentation of Two Sambucus Species with Aspergillus niger. Journal of Agricultural and Food Chemistry, 63(13), 3489-3500. https://doi.org/10.1021/acs.jafc.5b00520
  • Hammer, K.A., Carson, C.F., & Riley, T.V. (1999). Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology, 86, 985 990. https://doi.org/10.1046/j.1365-2672.1999.00780.x
  • Fernández-Agulló, E., Pereira, M., Freire, P., Valentao, P., Andrade, J., González-Álvarez, J., & Pereira, J.A. (2013). Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts. Industrial Crops and Products, 42, 126–132. https://doi.org/10.1016/j.indcrop.2012.05.021
  • Hammer, K.A., Carson, C.F., & Riley, T.V. (1999) Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology, 86(6), 985 990. https://doi.org/10.1046/j.1365-2672.1999.00780.x
  • Jaitak, V., Sharma, K., Kalia, K., Kumar, N., Singh, H.P., Kaul, V., & Singh, B., (2010). Antioxidant activity of Potentilla fulgens: An alpine plant of western Himalaya. Journal of Food Composition and Analysis, 23, 142-147. https://doi.org/10.1016/j.jfca.2009.02.013
  • Khuda, F., Haq, Z.U., Ilahi, I., Ullah, R., Khan, A., Fouad, H., Khalil, A.A.K., Ullah, Z., Sahibzada, M.U.K., Shah, Y., Abbas, M., Iftikhar, T., & Batiha, G.E.-S. (2021). Synthesis of gold nanoparticles using Sambucus wightiana extract and investigation of its antimicrobial, anti-inflammatory, antioxidant and analgesic activities. Arabian Journal of Chemistry, 14(10), 103343. https://doi.org/10.1016/j.arabjc.2021.103343
  • Kumar, V., Sharma, N., Sourirajan, A., Khosla, P.K., & Dev, K., (2018). Comparative evaluation of antimicrobial and antioxidant potential of ethanolic extract and its fractions of bark and leaves of Terminalia arjuna from north-western Himalayas, India. Journal of Traditional Complementary Medicine, 8, 100 106. https://doi.org/10.1016/j.jtcme.2017.04.002
  • Lee, T.B. (1980). Illustrated Flora of Korea. HyangMoonSa, Seoul, Korea. pp 702-703.
  • Milena, V., Tatjana, M., Gökhan, Z., Ivana, B., Aleksandra, C., Mohammad, M.F., & Marija, R., (2019). Advantages of contemporary extraction techniques for the extraction of bioactive constituents from black elderberry (Sambucus nigra L.) flowers. Industrial Crops and Products, 136, 93-101. https://doi.org/10.1016/j.indcrop.2019.04.058
  • Młynarczyk, K., Walkowiak-Tomczak, D., & Łysiak, G.P. (2018). Bioactive properties of Sambucus nigra L. as a functional ingredient for food and pharmaceutical industry. Journal of Functional Foods, 40, 377-390.Omar, H., Al-Judaibiand, A., & El-Gendy, A. (2018). Antimicrobial, antioxidant, anticancer activity and phytochemical analysis of the red alga, Laurencia papillosa. International Journal of Pharmacology, 14, 572 583. https://doi.org/10.3923/ijp.2018.572.583
  • Moreno, M.I.N., Isla, M.I., Sampietro, A.R., Vattuone, M.A. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology, 71, 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  • Rungruang, R., Ratanathavorn, W., Boohuad, N., Selamassakul, O., Kaisangsri, N. (2021). Antioxidant and anti-aging enzyme activities of bioactive compounds isolated from selected Zingiberaceae plants. Agriculture and Natural Resources, 55, 153 160. https://doi.org/10.34044/j.anres.2021.55.1.20
  • Tian, Y., Puganen, A., Alakomi, H.-L., Uusitupa, A., Saarela, M., & Yang, B. (2018). Antioxidative and antibacterial activities of aqueous ethanol extracts of berries, leaves, and branches of berry plants. Food Research International, 106, 291 303. https://doi.org/10.1016/j.foodres.2017.12.071
  • Velioglu, Y.S., Mazza, G., Gao, L., & Oomah, B.D. (1998). Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry, 46, 4113-4117.

In vitro antimicrobial and antioxidant activities of Sambucus williamsii and Sambucus pendula

Year 2024, Volume: 11 Issue: 2, 191 - 199, 03.06.2024
https://doi.org/10.21448/ijsm.1353669

Abstract

The present study aimed to compare the in vitro antimicrobial and antioxidant activities of Sambucus williamsii and Sambucus pendula. The antimicrobial activity of the two plants was evaluated using the disc diffusion and minimal inhibition concentration (MIC) method against three Gram-positive bacteria (Bacillus cereus ATCC 11778, Bacillus subtilis ATCC 9327 and Listeria monocytogenes ATCC 15313), four Gram-negative bacteria (Escherichia coli ATCC 15489, Psendomonas aeruginosa ATCC 27853, Pseudomonas fluorescens KCCM 41443 and Salmonella typhimurium KCCM 11862) and one yeast (Saccharomyces cerevisiae IFO 1950). The tested ether and ethyl acetate fractions of ethanol extract from Sambucus williamsii showed significant antimicrobial activity against Bacillus cereus and Pseudomonas fluorescens; conversely, the ether fraction of Sambucus pendula leaf showed no clear zone formation against any tested bacteria. In general, the fractions of the two Sambucus species exhibited a lower MIC against tested Gram-negative bacteria than the tested Gram-positive bacteria. The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay. The results showed that the half inhibitory concentration (IC50) for the ethanol extract of Sambucus williamsii was the lower value, which means the greater antioxidant activity. On the other hand, the IC50 value of the hot water extract of Sambucus pendula was the lower value. The richness of the total polyphenol contents of the two Sambucus implies their potential as raw material sources for the pharmaceutical and cosmeceutical industries.

References

  • Akhtar, N., Ihsan-ul-Haq, & Mirza, B. (2018). Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species Arabian Journal of Chemistry, 11(8), 1223-1235. https://doi.org/10.1016/j.arabjc.2015.01.013
  • Barak, T.H., Celep, E., İnan, Y., & Yeşilada, E. (2020) In vitro human digestion simulation of the bioavailability and antioxidant activity of phenolics from Sambucus ebulus L. fruit extracts. Food Bioscience, 37, 100711. https://doi.org/10.1016/j.fbio.2020.100711
  • Blois, M.S. (1958). Antioxidant determination by the use of a stable free radical. Nature, 26, 1199-1202.
  • Chae, J.W., & Cho, Y.J. (2012). Antioxidant activity of extracts from Sambucus williamsii var. coreana. Korean Journal of Plant Resources, 25(4), 363-371.
  • Ding, H.-Y., Chou, T.-H., Lin, R.-J., Chan, L.-P., Wang, G.-H., & Liang, C.-H. (2011). Antioxidant and antimelanogenic behaviors of Paeonia suffruticosa. Plant Foods for Human Nutrition, 66, 275–284. https://doi.org/10.1007/s11130-011-0235-3.
  • Dulf, F.V., Vodnar, D.C., Dulf, E.-H., & Toşa, M.I. (2015). Total Phenolic Contents, Antioxidant Activities, and Lipid Fractions from Berry Pomaces Obtained by Solid-State Fermentation of Two Sambucus Species with Aspergillus niger. Journal of Agricultural and Food Chemistry, 63(13), 3489-3500. https://doi.org/10.1021/acs.jafc.5b00520
  • Hammer, K.A., Carson, C.F., & Riley, T.V. (1999). Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology, 86, 985 990. https://doi.org/10.1046/j.1365-2672.1999.00780.x
  • Fernández-Agulló, E., Pereira, M., Freire, P., Valentao, P., Andrade, J., González-Álvarez, J., & Pereira, J.A. (2013). Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts. Industrial Crops and Products, 42, 126–132. https://doi.org/10.1016/j.indcrop.2012.05.021
  • Hammer, K.A., Carson, C.F., & Riley, T.V. (1999) Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology, 86(6), 985 990. https://doi.org/10.1046/j.1365-2672.1999.00780.x
  • Jaitak, V., Sharma, K., Kalia, K., Kumar, N., Singh, H.P., Kaul, V., & Singh, B., (2010). Antioxidant activity of Potentilla fulgens: An alpine plant of western Himalaya. Journal of Food Composition and Analysis, 23, 142-147. https://doi.org/10.1016/j.jfca.2009.02.013
  • Khuda, F., Haq, Z.U., Ilahi, I., Ullah, R., Khan, A., Fouad, H., Khalil, A.A.K., Ullah, Z., Sahibzada, M.U.K., Shah, Y., Abbas, M., Iftikhar, T., & Batiha, G.E.-S. (2021). Synthesis of gold nanoparticles using Sambucus wightiana extract and investigation of its antimicrobial, anti-inflammatory, antioxidant and analgesic activities. Arabian Journal of Chemistry, 14(10), 103343. https://doi.org/10.1016/j.arabjc.2021.103343
  • Kumar, V., Sharma, N., Sourirajan, A., Khosla, P.K., & Dev, K., (2018). Comparative evaluation of antimicrobial and antioxidant potential of ethanolic extract and its fractions of bark and leaves of Terminalia arjuna from north-western Himalayas, India. Journal of Traditional Complementary Medicine, 8, 100 106. https://doi.org/10.1016/j.jtcme.2017.04.002
  • Lee, T.B. (1980). Illustrated Flora of Korea. HyangMoonSa, Seoul, Korea. pp 702-703.
  • Milena, V., Tatjana, M., Gökhan, Z., Ivana, B., Aleksandra, C., Mohammad, M.F., & Marija, R., (2019). Advantages of contemporary extraction techniques for the extraction of bioactive constituents from black elderberry (Sambucus nigra L.) flowers. Industrial Crops and Products, 136, 93-101. https://doi.org/10.1016/j.indcrop.2019.04.058
  • Młynarczyk, K., Walkowiak-Tomczak, D., & Łysiak, G.P. (2018). Bioactive properties of Sambucus nigra L. as a functional ingredient for food and pharmaceutical industry. Journal of Functional Foods, 40, 377-390.Omar, H., Al-Judaibiand, A., & El-Gendy, A. (2018). Antimicrobial, antioxidant, anticancer activity and phytochemical analysis of the red alga, Laurencia papillosa. International Journal of Pharmacology, 14, 572 583. https://doi.org/10.3923/ijp.2018.572.583
  • Moreno, M.I.N., Isla, M.I., Sampietro, A.R., Vattuone, M.A. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology, 71, 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  • Rungruang, R., Ratanathavorn, W., Boohuad, N., Selamassakul, O., Kaisangsri, N. (2021). Antioxidant and anti-aging enzyme activities of bioactive compounds isolated from selected Zingiberaceae plants. Agriculture and Natural Resources, 55, 153 160. https://doi.org/10.34044/j.anres.2021.55.1.20
  • Tian, Y., Puganen, A., Alakomi, H.-L., Uusitupa, A., Saarela, M., & Yang, B. (2018). Antioxidative and antibacterial activities of aqueous ethanol extracts of berries, leaves, and branches of berry plants. Food Research International, 106, 291 303. https://doi.org/10.1016/j.foodres.2017.12.071
  • Velioglu, Y.S., Mazza, G., Gao, L., & Oomah, B.D. (1998). Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry, 46, 4113-4117.
There are 20 citations in total.

Details

Primary Language English
Subjects Basic Pharmacology
Journal Section Articles
Authors

Kyoung-sun Seo This is me 0000-0003-2397-5305

Kyeong Won Yun 0000-0002-9167-8348

Early Pub Date April 22, 2024
Publication Date June 3, 2024
Submission Date September 1, 2023
Published in Issue Year 2024 Volume: 11 Issue: 2

Cite

APA Seo, K.-s., & Yun, K. W. (2024). In vitro antimicrobial and antioxidant activities of Sambucus williamsii and Sambucus pendula. International Journal of Secondary Metabolite, 11(2), 191-199. https://doi.org/10.21448/ijsm.1353669
International Journal of Secondary Metabolite

e-ISSN: 2148-6905