Epigenetic factors of the effect of UV-C and X-ray presowing seeds radiation exposure in Matricaria chamomilla L. genotypes

Year 2024, Volume: 11 Issue: 2, 305 - 314, 03.06.2024

Daryna Sokolova , Alexandra Kravets Vladyslav Zhuk Ludmila Hlushchenko

https://doi.org/10.21448/ijsm.1358437

Abstract

In a series of experiments using both X-ray and UV-C radiation exposure a parallel study of several pharmacological characteristics of the Matricaria chamomilla L. genotype group was carried out. The data concerning the changes in the productivity of pharmacological raw materials and stimulation of the synthesis of low molecular weight antioxidants as markers of secondary metabolism induction have been published earlier. In this study, the data on the relationship between the stimulation of the synthesis of secondary metabolites under different types of irradiation and the epigenetic changes in the plant organism are presented. It was shown that DNA methylation was switched to the de novo mode in plants of all studied genotypes of M. chamomilla under both types of irradiation. That indicates changes in the epigenetic program of the plant organism. Comparison of the epigenetic pattern between control and irradiated samples, based on the difference in DNA methylation patterns in terms of a statistical indicator, shows that there is no unambiguous relationship between the epigenetic changes and increasing yield of antioxidant synthesis. This is additional evidence of the diversity of metabolic rearrangements and adaptive strategies of the plant organism under radiation exposure even within one species.

Keywords

UV-C, X-ray exposure, Remote effects, Secondary metabolites, Epigenetic distance

References

• Alothman, M., Bhat, R., & Karim, A.A. (2009). Effects of radiation processing on phytochemicals and antioxidants in plant produce. Trends in Food Science & Technology, 20(5), 201–212. https://doi.org/10.1016/j.tifs.2009.02.003
• Ausubel F.M. (2004) Current protocols in molecular biology. Biophotometer Operating Manual. http://www.eppendorf.com
• Сoleman, M.A., Yin, E., Peterson, L.E., Nelson, D., Sor-ensen, K., Tucker, J.D., & Wyrobek, A.J. (2005). Low dose irradiation alters the transcript profiles of human lymphoblastoid cells including genes associated with cytogenetic radioadaptive responses. Radiation Research, 164(41), 369-382. https://doi.org/10.1667/RR3356.1
• Dai, J., & Mumper, R.J. (2010). Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties. Molecules, 15(10), 7313-7352. https://doi.org/10.3390/molecule
• Danchenko, M., Skultety, L., Rashydov, N.M., Berezhna, V.V., Mátel, L., Salaj, T., Pret'ová, A., & Hajduch, M. (2009). Proteomic analysis of mature soybean seeds from the chernobyl area suggests plant adaptation to the contaminated environment. Journal of Proteome Research, 8(6), 2915-2922. https://doi.org/10.1021/pr900034u
• Flores, K.B., Wolschin, F., & Amdam, G.V. (2013). The role of methylation of DNA in environmental adaptation. Integrative and Comparative Biology, 53(2), 359-372. https://doi.org/10.1093/icb/ict019
• Hauser, M-T., Aufsatz, W., Jonak, C., & Luschnig, Ch. (2011). Transgenerational Epigenetic Inheritance in Plants. Biochimia Biophysica Acta, 1809(8), 459 468. https://doi.org/10.1016/j.bbagrm.2011.03.007
• Hernández, H.G., Tse, M.Y., Pang, S.C., Arboleda, H., & Forero, D.A. (2013). Optimizing methodologies for PCR-based DNA methylation analysis. BioTechniques, 55(4), 181–187. https://doi.org/10.2144/000114087
• Hassan, W., Noreen, H., Rehman, S., Gul, S., Kamal, M.A., Kamdem, J.P., Zaman, B., & da Rocha, J.B.T. (2017). Oxidative Stress and Antioxidant Potential of One Hundred Medicinal Plants. Current Topics in Medicinal Chemistry, 17(12), 1336 1370. https://doi.org/10.2174/1568026617666170102125648
• Jan, S., Parween, T., Siddiqi, T.O., & Mahmooduzzafar. (2012). Effect of gamma radiation on morphological, biochemical, and physiological aspects of plants and plant products. Environmental Reviews, 20(1), 17–39. https://doi.org/10.1139/a11-021
• Kaur, S., & Mondal, P. (2014). Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants. Journal of Microbiology and Experimentation, 1(1), 23-28. https://doi.org/10.15406/jmen.2014.01.00005
• Klein, F.R.S., Reis, A., Kleinowski, A.M., Telles, R.T., Amarante, L. do, Peters, J.A., & Braga, E.J.B. (2018). UV-B radiation as an elicitor of secondary metabolite production in plants of the genus Alternanthera. Acta Botanica Brasilica, 32(4), 615 623. https://doi.org/10.1590/0102-33062018abb0120
• Klubicova, K., Danchenko, M., Skultety, L., Berezhna, V.V., Rashydov, N.M., & Hajduch M. (2013). Radioactive chernobyl environment has produced high-oil flax seeds that show proteome alterations related to carbon metabolism during seed development. Journal of Proteome Research, 12(11), 4799–4806. https://doi.org/10.1021/pr400528m
• Kravets, A., & Sokolova, D. (2019). Epigenetic Factors of Biological Variability and Individual Sensitivity to Biotic Stresses. Global Journal of Science Frontier Research: C Biological Science, 20(1), 1-7. https://doi.org/10.1080/09553002.2020.1767819
• Kravets, A.Р., & Sokolova, D.A. (2020). Epigenetic factors of individual radiosensitivity and adaptive capacity. International Journal of Radiation Biology, 96(8), 999-1009. https://doi.org/10.1080/09553002.2020.1767819
• Sokolova, D.A., Kravets, A.P., & Vengzhen, G.S. (2013). An Analysis of the Correlation between the Changes in Satellite DNA Methylation Patterns and Plant Cell Responses to the Stress. CellBio, 2, 163-171. https://doi.org/10.4236/cellbio.2013.23018
• Kravets, A.P., Sokolova, D.A., Vengzhen, G.S., & Grodzinsky, D.M. (2013). Fractionated UV-C irradiation effects on the changes of transcribed and satellite DNA methylation profile and unstable chromosomal aberration yield. Radiation Biology Radioecology, 53(6), 583-592. https://pubmed.ncbi.nlm.nih.gov/25486740/
• Kravets, A.P., Sokolova, D.A., Zhuk, V.V., Sakada, V.I., Glushenko, L.A., & Kuchuk, M.V. (2021). The method of stimulating the synthesis of antioxidants in the raw materials of medicinal plants through pre-sowing UV-C irradiation of seeds. Patent of Ukraine No. 149151 [Data set]. Patent and trademark office. Kyiv, Ukraine. https://ukrpatent.org/uk/articles/bases2
• Nei, M. (1974). A new measure of genetic distance. Genetic distance. Plenum Press. https://doi.org/10.1111/j.1469-1809.1977.tb02032.x
• Ng, H.-H., & Bird, A. (1999). DNA methylation and chromatin modification. Current Opinion in Genetics & Development, 9, 158-163. https://doi.org/10.1016/s0959-437x(99)80024-0
• Nocchi, N., Duarte, H.M., Pereira, R.C., Konno, T.U.P., & Soares, A.R. (2020). Effects of UV-B radiation on secondary metabolite production, antioxidant activity, photosynthesis and herbivory interactions in Nymphoides humboldtiana (Menyanthaceae). Journal of Photochemistry and Photobiology B: Biology, 112-121. https://doi.org/10.1016/j.jphotobiol
• Shylina, Y.V, Pchelovska, S.V., & Litvinov, S.V. (2018). Method to increase flavonoid content in medicinal plant raw material with pre-sowing radiation exposure of seeds. Patent of Ukraine No. 129749 [Data set]. Patent and trademark office. Kyiv, Ukraine. https://ukrpatent.org/uk/articles/bases2
• Sokolova, D., Kravets, A., Zhuk, V., Sakada V., & Gluschenko, L. (2021). Productivity of medicinal raw materials by different genotypes of Matricia Chammomila L. is affected with pre-sowing radiation exposure of seeds. International Journal of Secondary Metabolites, 8(2), 127-135. https://doi.org/10.21448/ijsm.889817
• Teif, V.B. (2015). Nucleosome positioning: resources and tools online. Briefings in Bioinformatics, 17(15), 745-757. https://doi.org/10.1093/bib/bbv086
• Xu, J., Chen, G., Hermanson, P.J., Xu, Q., Sun, C., Chen, W., Kan, Q., Li, M., Crisp, P.A., Yan, J., Li, L., Springer, N.M., & Li, Q. (2019). Population–level analysis reveal the widespread occurrence and phenotypic consequence of DNA methylation variation not tagged by genetic variation in maize. Genome Biology, 20, 243-260. https://doi.org/10.1186/s13059-019-1859-0
• Zhuk, V., Sokolova, D., Kravets, A., Sakada, V., & Gluschenko, L. (2021). Efficiency of pre-sowing seeds by UV-C and X-ray exposure on the accumulation of antioxidants in inflorescence of plants of Matricaria chamomilla L. genotypes. International Journal of Secondary Metabolites, 8(3), 186–194. https://doi.org/10.21448/ijsm.889817