Carotenoid content, phytochemical screening, and antioxidant potential of Kantutay (Lantana camara L.), Katuray (Sesbania grandiflora L.), and Blue Ternate (Clitoria ternatea L.) flowers in the Philippines

Year 2024, Volume: 11 Issue: 3, 445 - 461

Melanie D. Piedad Lexter Natividad

https://doi.org/10.21448/ijsm.1389365

Abstract

Natural product chemistry plays a pivotal role in drug discovery and development. This study investigated the phytochemical profiles and antioxidant capacities of three prominent Philippine plants: Blue ternate (Clitoria ternatea), Kantutay (Lantana camara), and Katuray (Sesbania grandiflora). The primary aim is to provide a comprehensive assessment of their bioactive constituents and evaluate their potential for pharmacological applications. Phytochemical screening identified a diverse array of compounds, including flavonoids, tannins, glycosides, terpenoids, and other major chemical constituent classes, highlighting their therapeutic potential. The pigment analysis revealed substantial variations, with Blue ternate exhibiting the highest concentration, suggesting it as a promising source of carotenoids. Thin Layer Chromatography (TLC) and chlorophyll analysis further revealed distinct compound profiles. Total Phenolic Content (TPC) analysis and the DPPH radical scavenging method marked Blue ternate to have the highest phenolic content and the most potent antioxidant activity among the plant samples. These findings collectively emphasize the significant therapeutic potential of these plants, warranting further exploration for pharmaceutical development.

Keywords

Lantana camara, Sesbania grandiflora, Clitoria ternatea, Carotenoid, Phytochemicals, Antioxidant potential

References

• Albuquerque, B.R., Heleno, S.A., Oliveira, M.B.P., Barros, L., & Ferreira, I.C. (2021). Phenolic compounds: Current industrial applications, limitations, and future challenges. Food & Function, 12(1), 14-29. https://doi.org/10.1039/D0FO02324H
• Al-Snafi, E.A. (2016). Pharmacological importance of Clitoria Ternatea – A review. IOSR Journal of Pharmacy, 6(3), 68 83. ISSN: 2319 4219. https://www.researchgate.net/publication/313742374_Pharmacological_importance_of_Clitoria_Ternatea_-_A_review
• Arun, A., Karthikeyan, P., Sagadevan, P., Umamaheswari, R., & Rex Peo, R. (2014). Phytochemical screening of Sesbania grandiflora (Linn). International Journal of Biosciences and Nanosciences, 1(2), 33-36.
• Avalaskar, A.N., Itankar, P.R., Joshi, V.S., Agrawal, M., & Vyas, J. (2011). Phytochemical and TLC studies of ethanolic extract of Sesbania grandiflora (Fabaceae). International Journal of Pharmacology and Technology Research, 3(3), 1346-1349.
• Aykul, S., & Martinez-Hackert, E. (2016). Determination of the half-maximal inhibitory concentration using biosensor-based protein interaction analysis. Analytic Biochemistry, 508, 97-103. https://doi.org/10.1016/j.ab.2016.06.025
• Baessa, M., Rodrigues, M.J., Pereira, C., Santos, T., da Rosa Neng, N., Nogueira, J.M.F., Barreira, L., Varela, J., Ahmed, H., Asif, S., Boukhari, S.A., Kayani, W.K., Khawaja Shafique Ahmad, Zengin, G., A., Custódio, L. (2019). A comparative study of the in vitro enzyme inhibitory and antioxidant activities of Butea monosperma (Lam.) Taub. and Sesbania grandiflora (L.) Poiret from Pakistan: New sources of natural products for public health problems. South African Journal of Botany, 120, 146 156, https://doi.org/10.1016/j.sajb.2018.04.006
• Baluran, S.M., Natividad, L.R., Fernando, S.I., & Velasco, R.R. (2018a). Determination of pigments, antioxidant potential and vitamin contents of Benguet pine (Pinus kesiya) pollen. International Journal of Biology, Pharmacy, and Allied Sciences (IJBPAS), 7(9), 1643-1651. https://doi.org/10.31032/IJBPAS/2018/7.9.4517
• Baluran, S.M., Velasco, R.R., & Natividad, L.R. (2018b). Analyses of pigments, antioxidants, vitamins and immunostimulatory effect of Khasi pine (Pinus kesiya) pollen in Nile tilapia (Oreochromis niloticus L.). Ascendens Asia Journal of Multidisciplinary Research Abstracts, 2(5), 26. https://ojs.aaresearchindex.com/index.php/AAJMRA/article/view/3958
• Begum, S., Wahab, A., Siddiqui, B. S., & Qamar, F. (2000). Nematicidal constituents of the aerial parts of Lantana camara. Journal of Natural Products, 63(6), 765-767. https://doi.org/10.1021/np9903548
• Bhatt, I.D., Rawat, S., & Rawal, R.S. (2013). Antioxidants in medicinal plants. In: Chandra, S., LATA, H., Varma, A. (eds) Biotechnology for Medicinal Plants. Springer. https://doi.org/10.1007/978-3-642-29974-2_13
• Biology Dictionary. (2018). Carotenoids. https://biologydictionary.net/carotenoids/
• Bhokre, C., Gadhe, K., & Joshi, A. (2022). Assessment of nutritional properties of Sesbania grandiflora flower and leaves. The Pharma Innovation Journal, 11(6), 90-94.
• Brandi, M. L. (1992). Flavonoids: biochemical effects and therapeutic application. Bone and Mineral, 19(S1), S3-S14. https://doi.org/10.1016/0169-6009(92)90861-7
• Casuga, K.M.S., & Natividad, L.R. (2023). Comparative analysis of pigment contents, phytochemical constituents, and antioxidant properties of selected plants in the Philippines. International Research Journal of Science, Technology, Education, and Management, 3(4), 10-20.
• Chayaratanasin, P., Barbieri, M.A., Suanpairintr, N., & Adisakwattana, S. (2015). Inhibitory effect of Clitoria Ternatea flower petal extract on fructose-induced protein glycation and oxidation-dependent damages to albumin in vitro. BMC Complement Alternative Medicine, 15(1), 1-9. https://doi.org/10.1186/s12906-015-0546-2
• Chung, K.T., Wong, T.Y., Wei, C.I., Huang, Y.W., & Lin, Y. (2010). Tannins and human health: a review. Critical Reviews in Food Science and Nutrition, 38(6), 421-464. https://doi.org/10.1080/10408699891274273
• Day, M.D., Wiley, C.J., Playford, J., & Zalucki, M.P. (2003). Lantana: current management status and future prospects. Australian Centre for International Agricultural Research, Number 114054. https://doi.org/10.22004/ag.econ.114054
• Dethe, U.L., Joshi, S.S., Desai, S.S., & Aparadh, V.T. (2013). Screening of bioactive compounds of Sesbania grandiflora and Pistia stratiotes. Indian Journal of Advances in Plant Research, 1(1), 27-30.
• Dias, M.G., Camões, M.F., & Oliveira, L. (2008). Uncertainty estimation and in-house method validation of HPLC analysis of carotenoids for food composition data production. Food Chemistry, 109(4), 815-824. https://doi.org/10.1016/j.foodchem.2007.12.026
• El Aziz, M.M.A., Ashour, A.S., & Melad, A.S.G. (2019). A review on saponins from medicinal plants: chemistry, isolation, and determination. Journal of Nanomedicine Research, 7(4), 282-288. http://dx.doi.org/10.15406/jnmr.2019.07.00199
• Fernando, W.G. (2012). Plants: An international scientific open access journal to publish all facets of plants, their functions and interactions with the environment and other living organisms. Plants, 1(1), 1-5. https://doi.org/10.3390/plants1010001
• Garcia, N.G., Daigdigan, V.D., Landingin, E.J., Garcia, G., & Natividad, L. (2023). Antibacterial Effect of Basil (Ocimum basilicum) and Table Salt as Alternative Disinfectants against Staphylococcus aureus. Mindanao Journal of Science and Technology, 21(2), 140-152.
• Ghisalberti, E.L. (2000). Lantana camara L. (Verbenaceae). Fitoterapia, 71(5), 467-486. https://doi.org/10.1016/S0367-326X(00)00202-1
• Gollen, B., Mehla, J., & Gupta, P. (2018). Clitoria Ternatea Linn: A herb with potential pharmacological activities: Future prospects as therapeutic Herbal Medicine. Journal of Pharmacological Reports, 3(1), 141.
• Gomase, P., Gomase, P., Anjum, S., Shakil, S., & Shahnvaj, K.M. (2012). Sesbania sesban Linn: A review on its ethnobotany, phytochemical, and pharmacological profile. Asian Journal of Biomedical and Pharmaceutical Sciences, 2(12), 11-14.
• Goodman, M., Bostick, R.M., Kucuk, O., & Jones, D.P. (2011). Clinical trials of antioxidants as cancer prevention agents: Past, present, future. Free Radical Biology and Medicine, 51(5), 1068-1084. https://doi.org/10.1016/j.freeradbiomed.2011.05.018
• Gul, H., Nisa, Z., Jabeen, A., Maryam, & Sajad, M.A. (2020). An evaluation of phytochemical screening of Lantana camara Linn. (An invasive plant in Pakistan). Pure and Applied Biology, 9(3), 1856-1863. http://dx.doi.org/10.19045/bspab.2020.90198
• Gundran, N.L., Ramirez, L., Rodolfo, A.W., Natividad, L., & Alili, R. (2018). Antimicrobial resistance and molecular detection of resistant genes in Escherichia coli isolates in poultry using polymerase chain reaction (PCR) assay. Synergist, 1, 26-31.
• Hasan, N., Osman, H., Mohamad, S., Chong, W.K., Awang, K., & Zahariluddin, A.S.M. (2012). The chemical components of Sesbania grandiflora root and their antituberculosis activity. Pharmaceuticals, 5(8), 882-889. https://doi.org/10.3390/ph5080882
• Healthline. (2017). Carotenoids: Everything you need to know. https://www.healthline.com/health/carotenoids
• Hemalatha, T., & Kailasam, S.P. (2022). Carotenoid screening in selected flowers. Journal of Xi’an Shiyou University, Natural Science Edition, 18(12), 1160-1173.
• Janarny, G., Ranaweera, K.K.D.S., & Gunathilake, K.D.P.P. (2021). Antioxidant activity of hydro-methanolic extracts of Sri Lankan edible flowers. Biocatalysis and Agricultural Biotechnology, 35, 102081. https://doi.org/10.1016/j.bcab.2021.102081
• Khanbabaee, K., & Ree, T. (2001). Tannins: classification and definition. Natural Product Reports, 18(6), 641-649. https://doi.org/10.1039/b101061l
• Kato-Noguchi, H., & Kurniadie, D. (2021). Allelopathy of Lantana camara as an invasive plant. Plants, 10(5), 1028. https://doi.org/10.3390/plants10051028
• Kelemu. S., Cardona, C., & Segura, G. (2004). Antimicrobial and insecticidal protein isolated from seeds of Clitoria Ternatea, a tropical forage legume. Plant Physiology and Biochemistry, 42(11), 867-873. https://doi.org/10.1016/j.plaphy.2004.10.013
• Kirtikar, K.R., & Basu, B.D. (1995). Indian medicinal plants (2nd ed.). Bishen Singh and Mahendra pal singh, Allahabad, 2:084-1087.
• Kohli, R.K., Batish, D.R., Singh, H.P., & Dogra, K.S. (2006). Status, invasiveness and environmental threats of three tropical American invasive weeds (Parthenium hysterophorus L., Ageratum conyzoides L., Lantana camara L.) in India. Biological Invasion, 8, 1501-1510. https://doi.org/10.1007/s10530-005-5842-1
• Ludwiczuk, A., Skalicka-Woźniak, K., & Georgiev, M.I. (2017). Terpenoids. Pharmacognosy, 233-266. https://doi.org/10.1016/B978-0-12-802104-0.00011-1
• Malik, J.K., Manvi, F.V., Alagawadi, K.R., & Noolvi, M. (2008). Evaluation of anti-inflammatory activity of Gymnema sylvestre leaves extract in rats. International Journal of Green Pharmacy, 2(2), 114-115. https://doi.org/10.22377/ijgp.v2i2.42.
• Manzoor, M., Anwar, F., Sultana, B., & Mushtaq, M. (2013). Variation in antioxidant and antimicrobial activities in Lantana camara L. flowers in relation to extraction methods. Acta Scientiarum Polonorum Technologia Alimentaria, 12(3), 283 294. https://pubmed.ncbi.nlm.nih.gov/24584957/.
• Masinas, K., Reyes, R.F., Iglesi, P.I., Natividad, L., & Merculio, J. (2018). Pharmaceutical Grade Pectin from Chinese Honey Orange (Citrus poonensis) and Dalanghita (Citrus nobilis) Peelings. Synergist, 1, 62-67.
• Meléndez-Martínez, A.J., Benítez, A., Corell, M., Hernanz, D., Mapelli-Brahm, P., Stinco, C., & Coyago-Cruz, E. (2021). Screening for innovative sources of carotenoids and phenolic antioxidants among flowers. Foods, 10(11), 2625. https://doi.org/10.3390/foods10112625
• Moghimipour, E., & Handali, S. (2014). Saponin: properties, methods of evaluation, and applications. Annual Research and Review in Biology, 5(3), 207 220. http://dx.doi.org/10.9734/ARRB/2015/11674
• Munir, A.A. (1996). A taxonomic review of Lantana camara L. and L. montevidensis (Spreng.) Briq. (Verbenaceae) in Australia. Journal of the Adelaide Botanic Garden, 17, 1-27.
• National Nutritional Council. (2022). The health wonders of Blue Ternate. https://www.nnc.gov.ph/regional-offices/mindanao/region-ix-zamboanga-peninsula/8926-the-health-wonders-of-blue-Ternate
• Natividad, L., Astrero, M.F., Basinga, L., & Calang, M.K. (2014). Effects of Different Systemic Insecticides in Carotenoid Content, Antibacterial Activity and Morphological Characteristics of Tomato (Solanum lycopersicum var Diamante). Asia Pacific Journal of Multidisciplinary Research, 2(1), 209-213.
• Natividad, L., & Rafael, R. (2014). Carotenoid Analyses and Antibacterial Assay of Annato (Bixa orellana L.), Carrot (Daucus carota L.), Corn (Zea mays L.) and Tomato (Solanum lycopersicum L.) Extracts. Research Journal of Recent Sciences, 3(3), 40-45.
• Navarro, D.P., Del Rosario, C., Pangilinan, M.R. III, Natividad, L., & Cardenio, P. (2018). In Vitro Anthelmintic Property of Indigofera zollingeriana and Leucaena leucocephala Leaves Extracts against Gastro-Intestinal Nematodes in Goats (Capra aegagrus hircus). Synergist, 1, 18 – 25. https://ssrn.com/abstract=4220485
• Negi, G.C.S., Sharma, S., Vishvakarma, S.C.R., Samant, S.S., Maikhuri, R.K., Prasad, R.C., & Palni, L.M.S. (2019). Ecology and use of Lantana camara in India. The Botanical Review, 85, 109-130. https://doi.org/10.1007/s12229-019-09209-8
• Ortinero, C., Rafael, R., Rayos, C.E., Bautista, K., Feliciano, M.A., Natividad, L., Natividad, G. (2021). Distilled Spirit Extraction of Phenolic Antioxidants from Fruit and Vegetable Residues. Journal of Ecological Engineering, 22(1), 125 131. https://doi.org/10.12911/22998993/128864
• Paiva, S.A.R., & Russell, R.M. (1999). β – carotene and other carotenoids. Journal of the American College of Nutrition, 18(5), 426 433. https://doi.org/10.1080/07315724.1999.10718880
• Panawala, L. (2017). Difference between chlorophyll a and b. PEDIAA. Available online: http://pediaa.com/difference-between-chlorophyll-a-and-b/
• Panche, A.N., Diwan, A.D., & Chandra, S.R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5, e-47. https://doi.org/10.1017/jns.2016.41
• Pant, D.R., Pant, N.D., Saru, D.B., Yadav, U.N., & Khanal, D.P. (2017). Phytochemical screening and study of antioxidant, antimicrobial, antidiabetic, anti-inflammatory and analgesic activities of extracts from stem wood of Pterocarpus marsupium Roxburgh. Journal of Intercultural Ethnopharmacology, 6(2), 170 176. https://doi.org/10.5455/jice.20170403094055
• Purkait, M.K., Haldar, D., & Duarah, P. (2023). Chapter 2 – Utilization of various parts of the plant for extraction of phytochemicals and high-throughput screening techniques. Advances in Extraction and Applications of Bioactive Phytochemicals, 29 53. https://doi.org/10.1016/B978-0-443-18535-9.00004-1
• Ragupathy, S., & Newmaster, S.G. (2009). Valorizing the ‘Irulas’ traditional knowledge of medicinal plants in the Kodiakkarai Reserve Forest, India. Journal of Ethnobiology and Ethnomedicine, 5(10). https://doi.org/10.1186/1746-4269-5-10
• Roberts, M.F., & Wink, M. (1998). Alkaloids: Biochemistry, ecology, and medicinal applications. Springer Science + Business Media, LLC. https://doi.org/10.1007/978-1-4757-2905-4
• Rodriguez-Amaya, D.B. (2001). A Guide to Carotenoid Analysis in Foods. OMNI Research, ILSI Human Nutrition Institute, United States of America. Available online: https://pdf.usaid.gov/pdf_docs/Pnacq929.pdf
• Sardhara, R.A., & Gopal, S. (2013). Qualitative phytochemical screening of different solvent extracts of Tinospora cordifolia stem and Lantana camara flower. International Research Journal of Pharmaceutical and Applied Sciences, 3(5), 210-213.
• Serrano, J., Puupponen‐Pimiä, R., Dauer, A., Aura, A.M., & Saura‐Calixto, F. (2009). Tannins: current knowledge of food sources, intake, bioavailability and biological effects. Molecular Nutrition & Food Research, 53(S2), S310 S329. https://doi.org/10.1002/mnfr.200900039
• Sharma, O.P., Sharma, S., Pattabhi, V., Mahato, S.B., & Sharma, P.D. (2007). A review of the hepatotoxic plant Lantana camara. Critical Reviews in Toxicology, 37(4), https://doi.org/10.1080/10408440601177863
• Sieniawska, E., & Baj, T. (2017). Tannins. Pharmacognosy, 199 232. https://doi.org/10.1016/B978-0-12-802104-0.00010-X
• Singh, S.P. (1996). Biological control, in 50 years of crop. Science Research in India; Indian Council of Agricultural Research: New Delhi, India, 88-116.
• Srivastava, V., Khaan, S., & Banerjee, S. (2009). An evaluation of genetic fidelity of encapsulation microshoots of the medicinal plant: Cineraria maritime following six months of strong. Plant Cell, Tissue and Organ Culture, 99(2), 193-198. http://dx.doi.org/10.1007/s11240-009-9593-z
• Srivastava, P., Singh, M., Devi, G., & Chaturvedi, R. (2014). Chapter 30 – Herbal medicine and biotechnology for the benefit of human health. Animal Biotechnology, 563-575. https://doi.org/10.1016/B978-0-12-416002-6.00030-4
• Szalay, J. (2015). What are carotenoids? Live Science. https://www.livescience.com/52487-carotenoids.html
• Torres, R.C., Parcon, M.R.V., Esmundo, H.J.N., Canillo, D.C.P., & Ramil., C.C. (2021). Antioxidant activity and phytochemical constituents of Philippine Clitoria Ternatea flowers as a potential therapeutic agent against infectious diseases. Biological Sciences and Pharmaceutical Research, 10(2), 12-18. https://doi.org/10.15739/ibspr.22.003
• Udayangani, S. (2011). Difference between chlorophyll a and b. Difference Between website. https://www.differencebetween.com/difference-between-chlorophyll-a-and-vs-b/
• Veeresham, C. (2012). Natural products derived from plants as a source of drugs. Journal of Advanced Pharmaceutical Technology and Research, 3(4), 200 201. https://doi.org/10.4103/2231-4040.104709
• Velasco, R.R., Dollente, D.J.M., Natividad, L.R., & Abella, T.A. (2018). Benguet Pine Pollen (Pinus kesiya) as natural source of Phytoandrogen. International Journal of Biology, Pharmacy, and Allied Sciences (IJBPAS), 7(6), 1121–1132
• Wagh, V.A., Wagh, K.V., Tandale, Y.N., & Salve, S.A. (2009). Phytochemical, pharmacological and phytopharmaceutics aspects of Sesbania grandiflora (Hadga): A review. Journal of Pharmacy and Research, 2(5), 889-892.
• Weerasinghe, D., Upeka, K.A., Gunathilake, P., & Prasanna, K.D. (2020). Antioxidant properties of selected culinary flowers. Annals: Food Science & Technology, 21(4), 783-791. https://afst.valahia.ro/wp-content/uploads/2022/09/IV.2_Upeka.pdf