A comparative study of the antioxidant, antibacterial, and cytotoxic activities of different varieties of imported ripe Cavendish banana

Year 2024, Volume: 11 Issue: 4, 623 - 632

Faisal Said Hamed Al-abri Salem Said Jaroof Al Touby Mohammad Amzad Hossain

Abstract

Traditionally, Omanis used bananas to treat gastrointestinal disorders, constipation, and diarrhea. The study aims to determine the pharmacological and toxicological activities of two imported banana varieties collected from the local fruit market. Extracts were prepared separately by soaking the bananas in methanol for seventy-two hours. Then, the extracts were fractionated with various solvents with increasing patterns of polarity to give corresponding crude extracts. All extracts were used to determine their antioxidant, antibacterial, and cytotoxic activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH), agar gel diffusion, and brine shrimp lethality methods. In the Indian ripe bananas, the highest antioxidant activity was obtained from the ethyl acetate and the lowest in water crude extract. However, in the bananas from the Philippines, the highest activity was in chloroform extract and the lowest in water extract. Both extracts displayed moderate antibacterial activity at different concentrations. The range of inhibition was 0-19 mm against Gram-positive and negative bacterial strains. Both banana extracts showed significant cytotoxic activity at all working concentrations. Crude extracts killed all nauplii at the highest concentration of 500 μg/mL. In Indian bananas, the highest cytotoxic activity was found in the water crude extract with an LC50 value of 27.35μg/mL. The lowest was in ethyl acetate and methanol extracts with an LC50 value of 57.54 μg/mL. Almost similar results were obtained from the Philippines. In conclusion, the polar crude extracts prepared from both varieties of ripe bananas have significant pharmacological and toxicological activities. Therefore, polar banana extracts might be agents that can be used as antibiotics.

Keywords

Cavendish banana, Crude extracts, Antioxidant activity, Antibacterial activity, Cytotoxicity activity

Project Number

N/A

Thanks

We are grateful to the University of Nizwa, Nizwa, Sultanate of Oman, for providing all logistic support to complete the present research. We also thank the staff of the Pharmaceutical Chemistry Laboratory for their assistance in carrying out the project successfully. Finally, thanks to the Writing Center (TWC) for their professional assistance throughout the writing process of my research project.

References

• Adinarayana, K.P.S., & Babu, A.P. (2011). Antioxidant activity and cytotoxicity of ethanolic extracts from the rhizome of Musa acuminata. Natural Science, 3(4), 291-294.
• Ahmed, M.A.E., Zeinab, A.S., Alaa, A.G., Hanan, F.A., Faten, A.E., & Habiba, A.A. (2016). Identification of phenolic compounds from banana peel (Musa paradaisica L.) as antioxidant and antibacterial agents. Journal of Chemical and Pharmaceutical Research, 8(4), 46-55.
• Anilreddy, B. (2009). Preparation, characterization and biological evaluation of some overview of Dodonaea viscosa Linn. Journal of Pharmaceutical Science and Technology, 1(1), 1-9.
• Al Hadhrami, R.M.S., & Hossain, M.A. (2016). Evaluation of antioxidant, antibacterial and cytotoxic activities of seed crude extracts of Ammi majus grown in Oman. Egyptian Journal of Basic and Applied Sciences, 3(4), 329-334.
• Al-matani, S.K., Al-Wahaibi, R.N.S., & Hossain, M.A. (2015). In vitro evaluation of the total phenolic and flavonoid contents and the antibacterial and cytotoxicity activities of crude fruit extracts with different polarities from Ficus sycomorus.Pacific Science Review A: Natural Science and Engineering, 17(3), 103-108.
• Ayoola-Oresanya, I.O., Sonibare, M.A., Gueye, B., Rajneesh Paliwal, R., Abberton, M.T., & Morlock, G.E., (2020), Effect-directed profiling and identification of bioactive metabolites from field, in vitro-grown and acclimatized Musa spp. accessions using high-performance thin-layer chromatography-mass spectrometry. Journal of Chromatography A, 1616(4), 460774.
• Aziza, S.R.A., & Hossain, M.A. (2015). Study on total phenolics and antioxidant activity of leaves crude extracts of Annona squamosal traditionally used for the treatment of cancerous tumours. Asian Pacific Journal of Tropical Disease, 5(Suppl 1), S142-S144.
• Barroso, W.A., Abreu, I.C., Ribeiro, L.S., Rocha, C.Q., de Souza, H.P., & Lima, T.M. (2019). Chemical composition and cytotoxic screening of Musa cavendish green peels extract: Antiproliferative activity by activation of different cellular death types. Toxicology In Vitro, 59, 179-186.
• Daimari, M., & Swargiary, A. (2020). Study of Phytochemical content and antioxidant properties of Musa balbisiana Corm Extract. Indian Journal of Pharmaceutical Sciences, 82(4), 707-712.
• Debabandya, M., Sabyasachi, M., & Namrata, S. (2010). Banana and its byproducts utilization: An overview. Journal Science Indian Research, 69, 323-29.
• Fagbemi, J.F., Ugoji, E., Adenipekun, T., & Adelowotan, O. (2009). Evaluation of the antimicrobial properties of unripe banana (Musa sapientum L.), lemon grass (Cymbopogon citratus S.) and turmeric (Curcuma longa L.) on pathogens. African Journal of Biotechnology, 8(7), 1176-1182.
• Fernanda, R.R., Andréa, F.A., Egle, M.A.S., & Sandra F.A. (2016). Phytochemical compounds and antioxidant capacity of tucum-do-cerrado (Bactris setosa Mart), Brazil’s Native Fruit. Nutrients, 8, 109-120
• Juliana, M.C., Bárbara, H.M., Prado, E.F., & Amanda, L.T.D. (2014). Evaluation of antimicrobial and cytotoxic activities of plant extracts from southern minas gerais cerrado. Revista do Instituto de Medicina Tropical de São Paulo, 58(1), 13-20.
• Liu, R.H. (2004). Potential synergy of phytochemicals in cancer prevention: mechanism of action. The Journal of Nutrition, 134(12), 3479S-3485S.
• Natcharee, P., & Sudip, K.R. (2011), Physical and antimicrobial properties of banana flour/chitosan biodegradable and self-sealing films used for preserving fresh-cut vegetables. LWT - Food Science and Technology, 44(10), 2310-2315.
• Nessma, A.Z (2015), Antioxidant, antitumor, antimicrobial studies and quantitative phytochemical estimation of ethanolic extracts of selected fruit peels. International Journal of Current Microbiology and Applied Sciences, 4(5), 298-309.
• Oliveira, L., Freire, C.S., Silvestre, A.J., & Cordeiro, N. (2008). Lipophilic extracts from banana fruit residues: a source of valuable phytosterols. Journal of Agricultural and Food Chemistry, 56(20), 9520-9524.
• Pereira, A., & Maraschin, M. (2015). Banana (Musa spp.) from peel to pulp: ethnopharmacology, source of bioactive compounds and its relevance for human health. Journal of Ethnopharmacology, 160, 149-163.
• Rafaela, G.M., Gloria, M.L., Monica, G. (2010). Antioxidant activity in banana peel extracts: Testing extraction conditions and related bioactive compounds. Food Chemistry, 119(3),1030-1039.
• Ramzi, A., Mothana, A., Salah, A., Hasson, S., Althawab, F., Alaghbari, S., & Lindequist, U. (2010). Antimicrobial, antioxidant and cytotoxic activities and phytochemical screening of some Yemeni medicinal plants. Complementary Alternative Medicine, 7(3), 323–325.
• Rao, N.M., Prasad, S.H.K.R., & Jyothirmayi, N. (2012). Efficacy of ripened and unripenedfruit extracts of Musa paradisiaca L (Bontha cultivar) against human pathogens. International Journal of Pharmacy and Pharmaceutical, 4(1), 454-460.
• Rao, M., Muhammad, A., & Khamsah, S.M. (2016). Phytochemical screening, total flavonoid and phenolic content assays of various solvent extracts of tepal of Musa paradisiaca. Malaysian Journal of Analytical Sciences, 20(5), 1181-1190
• Reinisalo, M., Kårlund, A., Koskela, A., Kaarniranta, K., & Karjalainen, R.O. (2015). Polyphenol stilbenes: molecular mechanisms of defence against oxidative stress and aging-related diseases. Oxidative Medical Cell Longev, 1–24.
• Tahiya, H., Amira, H., Hossain, M., Weli, A., & Al-Riyami, Q. (2014). Comparative study of phytochemical screening, antioxidant and antimicrobial capacities of fresh and dry leaves crude plant extracts of Datura metel L. Journal of King Suad University-Science, 26(3), 237-243.
• Weli, A.M., AL-Hinai, J.R., Al-Mjrafi, J.M., Alnaaimi, J.R., Hossain, M.A., Saeed, S., & Aktar, M.S. (2014). Effect of different polarities leaves crude extracts of Omani Juniperus excelsa in antioxidant, antimicrobial, and cytotoxic activities and their biochemical screening. Asian Pacific Journal of Reproduction, 3(3), 218-223.
• Zafar, I.M., Saleha, A., Hoque, M.M.E., & Sohel, R.M. (2011). Antimicrobial and cytotoxic properties of different extracts of Musa sapientum L. subsp. sylvestris. International Research Journal of Pharmacy, 2, 62-65.