Cytotoxic effect of Trigonella coerulescens subsp. ayvalikensis Erdoğan, Selvi & Tümen in prostate and colon cancer cell lines

Year 2024, Volume: 11 Issue: 4, 700 - 709, 03.11.2024

Sümeyye Aydoğan Türkoğlu , Selin Koç Fatma Poyrazlı Selami Selvi

https://doi.org/10.21448/ijsm.1485930

Abstract

Trigonella coerulescens subsp. ayvalikensis is an annual local endemic taxon distributed only in Ayvalık/Balıkesir district and evaluated in the Vulnerable Category (VU). Trigonella L. is an important genus with medicinal and economic value in the Fabaceae family. Seeds of the genus Trigonella are known to contain several groups of secondary metabolites, the most abundant compounds being steroidal saponins, as well as flavones, isoflavones, and polysaccharides. In our study, the cytotoxic effect on two different cancer cell lines namely, PC-3 (prostate) and SW480 (colon), was investigated by extracting Trigonella coerulescens subsp. ayvalikensis with different solvents. The cytotoxic effects of extracts obtained from plant seeds with different solvents (hexane, methanol, ethanol, acetone) were investigated. The MTT test was used to examine the cytotoxic effect, which was studied with PC-3 and SW480 cancer cell lines. The different concentrations (23.45 µg/µL, 46.875 µg/µL, 93.75 µg/µL, 187.5 µg/µL, 375 µg/µL) of seed extracts were applied to the cells at different times points (24h, 48h and 72h) and absorbance was taken at 550 nm in the spectrophotometer. As a result of cytotoxic studies, it was observed that hexane extract had the most reducing effect on PC-3 compared to the control groups. In the SW480 cell line, a proliferative effect was observed in extracts prepared with methanol, hexane, and acetone in the early period of 24 hours. In the later period (72 hours), the extract prepared with hexane and acetone showed the most cytotoxic effect on SW480 cells.

Keywords

Trigonella, Prostate cancer, Colon cancer, Cytotoxicity

Project Number

-

References

• Abas, A.S.M., & Naguib, D.M. (2019). Effect of germination on anticancer activity of Trigonella foenum seeds extract. Biocatalysis and agricultural biotechnology, 18, 101067. https://doi.org/10.1016/j.bcab.2019.101067
• Ahmed, S.I., Hayat, M.Q., Zahid, S., Tahir, M., Mansoor, Q., Ismail, M., Keck, K., & Bates, R. (2017). Isolation and identification of flavonoids from anticancer and neuroprotective extracts of Trigonella foenum graecum. Tropical Journal of Pharmaceutical Research, 16(6), 1391-1398. https://doi.org/10.4314/tjpr.v16i6.25
• Akan, H., Ekici, M., & Aytac, Z. (2020). The synopsis of the genus Trigonella L. (Fabaceae) in Turkey. Turkish Journal of Botany, 44(6), 670-693. https://doi.org/10.3906/bot-2004-63
• Akinmoladun, A.C., Falaiye, O.E., Ojo, O.B., Adeoti, A., Amoo, Z.A., & Olaleye, M.T. (2022). Effect of extraction technique, solvent polarity, and plant matrix on the antioxidant properties of Chrysophyllum albidum G. Don (African Star Apple). Bulletin of the National Research Centre, 46(1), 40. https://doi.org/10.1186/s42269-022-00718-y
• Al-Dabbagh, B., Elhaty, I.A., Al Hrout, A.A., Al Sakkaf, R., El-Awady, R., Ashraf, S.S., & Amin, A. (2018). Antioxidant and anticancer activities of Trigonella foenum-graecum, Cassia acutifolia and Rhazya stricta. BMC complementary and alternative medicine, 18, 1-12. https://doi.org/10.1186/s12906-018-2285-7
• Allaoui, A., Gascón, S., Benomar, S., Quero, J., Osada, J., Nasri, M., Rodriguez-Yoldi, M.J., & Boualga, A. (2019). Protein hydrolysates from fenugreek (Trigonella foenum graecum) as nutraceutical molecules in colon cancer treatment. Nutrients, 11(4), 724. https://doi.org/10.3390/nu11040724
• Baytop, T. (1999). Türkiye’de bitkiler ile tedavi: Geçmişte ve bugün (2nd ed.). [Treatment with plants in Turkey: Past and present]. Nobel Tıp Kitabevleri, ISBN: 975-420-021- 1. İstanbul, 480s. (in Turkish).
• Çoklar, H., & Akbulut, M. (2016). Alıç (Crataegus orientalis) meyvesinin antioksidan aktivitesi ve fenolik bileşiklerinin ekstraksiyonu üzerine farklı çözgenlerin etkisi [Effect of different solvents on extraction of phenolic compounds and antioxidant activity of hawthorn (Crataegus orientalis) fruits]. Derim, 33(2), 237 248. https://doi.org/10.16882/derim.2016.267908
• Cömert Önder, F., Ay, M., Aydoğan Türkoğlu, S., Tura Köçkar, F., & Celik, A. (2016). Antiproliferative activity of Humulus lupulus extracts on human hepatoma (Hep3B), colon (HT-29) cancer cells and proteases, tyrosinase, β-lactamase enzyme inhibition studies. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(1), 90 98. https://doi.org/10.3109/14756366.2015.1004060
• Dzah, C.S., Duan, Y., Zhang, H., Wen, C., Zhang, J., Chen, G., & Ma, H. (2020). The effects of ultrasound assisted extraction on yield, antioxidant, anticancer and antimicrobial activity of polyphenol extracts: A review. Food Bioscience, 35, 100547. https://doi.org/10.1016/j.fbio.2020.100547
• Erdoğan, E., Selvi, S., & Tümen, G. (2017). Trigonella coerulescens subsp. ayvalikensis (Fabaceae), a new taxon from Balıkesir, western Anatolia. Phytotaxa, 319(2), 167-174. https://doi.org/10.11646/phytotaxa.319.2.5
• Goyal, S., Gupta, N., Kumar, A., Chatterjee, S., & Nimesh, S. (2018). Antibacterial, anticancer and antioxidant potential of silver nanoparticles engineered using Trigonella foenum‐graecum seed extract. IET Nanobiotechnology, 12(4), 526-533. https://doi.org/10.1049/iet-nbt.2017.0089
• Güner, A., Aslan, S., Ekim, T., Vural, M., & Babaç, M.T. (2012). Türkiye bitkileri listesi (damarlı bitkiler) [List of plants in Turkey (vascular plants)]. Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını. (in Turkish).
• Habib-Martin, Z.A., Hammad, H.M., Afifi, F.U., Zihlif, M., Al-Ameer, H.J., Saleh, M.M., Abaza, I.F. & Nassar, Z.D. (2017). In vitro and in vivo evaluation of the antiangiogenic activities of Trigonella foenum-graecum extracts. Asian Pacific Journal of Tropical Biomedicine, 7(8), 732-738. https://doi.org/10.1016/j.apjtb.2017.07.013
• Iranmanesh, M., Mohebbati, R., Forouzanfar, F., Roshan, M.K., Ghorbani, A., Nik, M.J., Soukhtanloo, M. (2018). In vivo and in vitro effects of ethanolic extract of Trigonella foenum-graecum L. seeds on proliferation, angiogenesis and tube formation of endothelial cells. Research in Pharmaceutical Sciences, 13(4), 343-352, https://doi.org/10.4103/1735-5362.235161
• Koç, H. (2002). Bitkilerle sağlıklı yaşama [Healthy living with plants]. Kültür Eserleri Dizisi. ISBN: 975-17-2925-4. (in Turkish)
• Król-Kogus, B., Głód, D., & Krauze-Baranowska, M. (2020). Qualitative and quantitative HPLC-ELSD-ESI-MS analysis of steroidal saponins in fenugreek seed. Acta Pharmaceutica, 70(1), 89-99. https://doi.org/10.2478/acph-2020-0013
• Krol-Kogus B, Hałasa R, Głod D, Krauze-Baranowska M and Pobłocka-Olech L. (2021). 2D LC as a tool for standardization of Foenugraeci semen extracts containing compounds with anti Helicobacter pylori activity, Food Function, 12(6), 2686. https://doi.org/10.1039/D1FO00226K
• Mahapatra, K., Ghosh, A.K., De, S., Ghosh, N., Sadhukhan, P., Chatterjee, S., Ghosh, R., Sil, P.C., & Roy, S. (2020). Assessment of cytotoxic and genotoxic potentials of a mononuclear Fe (II) Schiff base complex with photocatalytic activity in Trigonella. Biochimica et Biophysica Acta (BBA) General Subjects, 1864(3), 129503. https://doi.org/10.1016/j.bbagen.2019.129503
• Nawaz, H., Shad, M.A., Rehman, N., Andaleeb, H., & Ullah, N. (2020). Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds. Brazilian Journal of Pharmaceutical Sciences, 56, e17129. http://dx.doi.org/10.1590/s2175-97902019000417129
• POWO. (2024). Trigonella L. Plants of the World Online. Royal Botanic Gardens, Kew. https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:30107635-2 (Accessed July 15, 2022).
• Ribeiro, D.A., Camilo, C.J., Nonato, C.D.F.A., Rodrigues, F.F.G., Menezes, I.R.A., Ribeiro-Filho, J., Xiao, J., Almeida Souza, M.M., & da Costa, J.G.M. (2020). Influence of seasonal variation on phenolic content and in vitro antioxidant activity of Secondatia floribunda A. DC. (Apocynaceae). Food Chemistry, 315, 126277. https://doi.org/10.1016/j.foodchem.2020.126277
• Salem, N., Msaada, K., Hamdaoui, G., Limam, F., & Marzouk, B. (2011). Variation in phenolic composition and antioxidant activity during flower development of safflower (Carthamus tinctorius L.). Journal of Agricultural and Food Chemistry, 59(9), 4455–4463. https://doi.org/10.1021/jf1049134
• Stefanowicz-Hajduka, J., Krol-Kogus, B., Sparzak-Stefanowskab, B., Kimelb, K., Ochockaa J. R., Krauze-Baranowska, M. (2021). Cytotoxic activity of standardized extracts, a fraction, and individual secondary metabolites from fenugreek seeds against SKOV-3, HeLa and MOLT 4 cell lines. Pharmaceutıcal Biology, 59(1), 424 437. https://doi.org/10.1080/13880209.2021.1903047
• Thakur, R.S., & Ahirwar, B. (2019). A steroidal derivative from Trigonella foenum-graecum L. that induces apoptosis in vitro and in vivo. Journal of Food and Drug Analysis, 27(1), 231–239. https://doi.org/10.1016/j.jfda.2018.05.001
• Tokur, O., & Aksoy, A. (2017). In vitro sitotoksisite testleri. Harran Üniversitesi Veterinerlik Fakültesi Dergisi, 6(1), 112-118. https://dergipark.org.tr/tr/download/article-file/320938