Comprehensive analysis of antibacterial and antioxidant properties in Calotropis gigantean leaf extracts

Year 2025, Volume: 12 Issue: 1, 33 - 45

Hazqail Umar Khan Muhammad Shahid Cholistani Eliza Iqbal Kashif Kareem , Hafiz Muhammad Kashif Zahoor Muhammad Farhan Hafiz Shozab Ahmad Khan Muhammad Pervaiz Bhatti

Abstract

Calotropis gigantea Linn., commonly known as arka, is a wild tropical plant from the Asclepiadacea family with various medicinal properties. Previous studies have stated its antioxidant and antibacterial properties. For this purpose, leaf extracts of C. gigantea were prepared using a variety of solvents: ethanol, methanol, and n-hexane. For phytochemical bioactive compounds identification LC-MS analysis was performed, and to evaluate antibacterial activity against different gram positive (Bacillus subtilis, Bacillus muralis) and gram negative (E. coli, Acetobactor rhizospherensis) bacterial strains well diffusion method was applied. Antioxidant activity was observed using ABTS (2,2′-azino-bis-(3-ethylenebenzothiazoline)-6-sulfonic acid assay) and DPPH (2,2-diphenylpicrylhydrazyl assa) assays. The LC-MS (liquid chromatography-mass spectrometry) analysis revealed that ethanol leaf extract contained the highest number of compounds (14), while a 50% ethanol-methanol mixture had the fewest. In antimicrobial activity tests, methanol leaf extract exhibited the greatest inhibition zone against Bacillus subtilis (8 mm), and n-hexane the smallest (2 mm). Ethanol leaf extract had the highest inhibition against E. coli (9.5 mm), with methanol showing the lowest (4 mm). For Acetobacter rhizopherensis, ethanol extract demonstrated the largest inhibition zone (7.75 mm), while n-hexane showed the smallest (6 mm). Against Bacillus muralis, n-hexane showed the highest inhibition (5.75 mm), and methanol the lowest (3.5 mm). The ABTS assay indicated that ethanol extract had the highest inhibition activity (9.73%), and n-hexane the lowest (5.64%). The DPPH assay revealed that methanol extract had the highest radical remaining activity (99.68%), with n-hexane having the lowest (97.19%). C. gigantea is a prospective source of antioxidant and antibacterial agent.

Keywords

Calotropis gigantea, Leaf extract, Antibacterial, Antioxidant, LC-MS

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References

• Al-Maskri, A.Y., Hanif, M.A., Al-Maskari, M.Y., Abraham, A.S., Al-sabahi, J.N., & Al-Mantheri, O. (2011). Essential oil from Ocimum basilicum (Omani Basil): a desert crop. Natural product communications, 6(10), 1934578X1100601020.
• Alam, M.A., Habib, M.R., Farjana, N., Khalequzzaman, M., & Karim, M.R. (2009). Insecticidal activity of root bark of Calotropis gigantea L. against Tribolium castaneum (Herbst). World Journal of Zoology, 4(2), 90-95.
• Alam, M.A., Habib, M.R., Nikkon, R., Rahman, M., & Karim, M.R. (2008). Antimicrobial Activity of Akanda (Calotropis gigantea L.) on Some Pathogenic Bacteria. Bangladesh Journal of Scientific and Industrial Research, 43(3), 397-404.
• Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G., & Lightfoot, D.A. (2017). Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6(4), 42.
• Argal, A., & Pathak, A.K. (2006). CNS activity of Calotropis gigantea roots. Journal of Ethnopharmacology, 106(1), 142-145.
• Bhatia, S., Al-Harrasi, A., Kumar, A., Behl, T., Sehgal, A., Singh, S., Sharma, N., Anwer, M. K., Kaushik, D., & Mittal, V. (2022). Anti-migraine activity of freeze-dried latex obtained from Calotropis gigantea Linn. Environmental Science and Pollution Research, 1-19.
• Borah, S., Sarkar, P., & Sharma, H.K. (2020). Chemical Profiling, Free Radical Scavenging and Anti acetylcholinesterase Activities of Essential Oil from Curcuma caesia of Arunachal Pradesh, India. Pharmacognosy Research, 12(1).
• Chitme, H., Chandra, R., & Kaushik, S. (2005). Evaluation of antipyretic activity of Calotropis gigantea (Asclepiadaceae) in experimental animals. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 19(5), 454-456.
• Chitme, H.R., Chandra, R., & Kaushik, S. (2004). Studies on anti-diarrhoeal activity of Calotropis gigantea R. Br. in experimental animals. J Pharm Pharm Sci, 7(1), 70-75.
• Choudhary, N., Siddiqui, M., Azmat, S., & Khatoon, S. (2013). Tinospora cordifolia: ethnobotany, phytopharmacology and phytochemistry aspects. International Journal of Pharmaceutical Sciences and Research, 4(3), 891.
• Deshmukh, P.T., Fernandes, J., Atul, A., & Toppo, E. (2009). Wound healing activity of Calotropis gigantea root bark in rats. Journal of Ethnopharmacology, 125(1), 178-181.
• Gyawali, R., Bhattarai, B., Bajracharya, S., Bhandari, S., Bhetwal, P., Bogati, K., … Joshi, R. (2020). α-amylase inhibition, antioxidant activity and phytochemical analysis of Calotropis gigantea (L.) Dryand. Journal of Health and Allied Sciences, 10(1), 77-81.
• Habib, M.R., & Karim, M.R. (2009). Antimicrobial and cytotoxic activity of di-(2-ethylhexyl) phthalate and anhydrosophoradiol-3-acetate isolated from Calotropis gigantea (Linn.) flower. Mycobiology, 37(1), 31-36.
• Julius, O., Oluwasusi, V., Ibiyemi, M., & Oluwatobi, F. (2021). Antibacterial Activities and Phytochemical Screening of Crude Extracts of Calotropis gigantea (Giant Milk Weed). South Asian Journal of Research in Microbiology, 9(3), 24-31.
• Klūga, A., Terentjeva, M., Vukovic, N.L., & Kačániová, M. (2021). Antimicrobial activity and chemical composition of essential oils against pathogenic microorganisms of freshwater fish. Plants, 10(7), 1265.
• Kumar, G., Karthik, L., & Rao, K.B. (2010a). Antibacterial activity of aqueous extract of Calotropis gigantea leaves–an in vitro study. International journal of pharmaceutical Sciences Review and Research, 4(2), 141-144.
• Kumar, G., Karthik, L., & Rao, K.B. (2010b). Antimicrobial activity of latex of Calotropis gigantea against pathogenic microorganisms-an in vitro study. Pharmacologyonline, 3(3), 155-163.
• Kumar, P.S., Chezhian, A., Raja, P.S., & Sathiyapriya, J.S. (2012). Computational selections of terpenes present in the plant Calotropis gigantea as mosquito larvicide’s by blocking the sterol carrying protein, AeSCP-2. Bangladesh Journal of Pharmacology, 7(1), 1-5.
• Lodhi, G., Kumar Singh, H., Pant, K.K., & Hussain, Z. (2009). Hepatoprotective effects of Calotropis gigantea extract against carbon tetrachloride induced liver injury in rats. Acta Pharmaceutica, 59(1), 89-96.
• Mahajan, R., & Badgujar, S. (2008). Phytochemical investigations of some laticiferous plants belonging to Khandesh region of Maharashtra. Ethnobotanical Leaflets, 2008(1), 151.
• Mandal, B.K., Mandal, R., Limbu, D., Adhikari, M.D., Chauhan, P.S., & Das, R. (2022). Green synthesis of AgCl nanoparticles using Calotropis gigantea: characterization and their enhanced antibacterial activities. Chemical Physics Letters, 801, 139699.
• Merzaia, A.B., Riaz, H., Rehman, R., Nisar, S., & Azeem, M.W. (2017). A review of toxicity, therapeutic and biological activities of Calotropis. IJCBS, 112017, 58-64.
• Muhammad, A.H., Masoud, Y.A.-M., Awatif, A.-M., Ahmed, A.-S., Ahmed, Y.A.-M., & Jamal, N.A.-S. (2011). Essential oil composition, antimicrobial and antioxidant activities of unexplored Omani basil. Journal of Medicinal Plants Research, 5(5), 751-757.
• Nalwaya, N., Pokharna, G., Deb, L., & Jain, N. K. (2009). Wound healing activity of latex of Calotropis gigantea. Int J Pharm Pharm Sci., 1(1), 176-181.
• Namrata, S., Jain, N.K., Pushpendra, K., Navneet, G., Pathak, A.K., & Mehta, S.C. (2010). In vitro antioxidant activity of Calotropis gigantea hydroalcohlic leaves extract. Der Pharmacia Lettre, 2(3), 95-100.
• Osungunna, M.O. (2021). Screening of medicinal plants for antimicrobial activity: Pharmacognosy and microbiological perspectives. Journal of Microbiology, Biotechnology and Food Sciences, 2021, 727-735.
• Patel, H.V., Patel, J.D., & Patel, B. (2014). Comparative efficacy of phytochemical analysis and antioxidant activity of methanolic extract of Calotropis gigantea and Calotropis procera. Int J Life Sci Biotechnol Pharm Res, 5(2), 107-113.
• Pathak, A., & Argal, A. (2007). Analgesic activity of Calotropis gigantea flower. Fitoterapia, 78(1), 40-42.
• Rajamohan, S., Kalaivanan, P., Sivangnanam, H., & Rajamanickam, M. (2014). Antioxidant, Antimicrobial activities and GC-MS analysis of Calotropis gigantea white flowers. The Journal of Phytopharmacology, 3(6), 405-409.
• Rajesh, R., Gowda, C.R., Nataraju, A., Dhananjaya, B., Kemparaju, K., & Vishwanath, B. (2005). Procoagulant activity of Calotropis gigantea latex associated with fibrin (ogen) olytic activity. Toxicon, 46(1), 84-92.
• Rani, R., Sharma, D., Chaturvedi, M., & Yadav, J.P. (2019). Phytochemical analysis, antibacterial and antioxidant activity of Calotropis procera and Calotropis gigantea. The Natural Products Journal, 9(1), 47-60.
• Sadowska-Bartosz, I., & Bartosz, G. (2022). Evaluation of the antioxidant capacity of food products: Methods, applications and limitations. Processes, 10(10), 2031.
• Sangeetha, K., Steffi, P., Selvi, B.T., & Priyadarshni, S. (2020). Phytochemical Evaluation, GC-MS Analysis of Phytoactive Compounds and Antibacterial Activity Studies from Calotropis gigantea. Journal of Pharmaceutical Sciences and Research, 12(6), 789-794.
• Shahzadi, I., Nadeem, R., Hanif, M.A., Mumtaz, S., Jilani, M.I., & Nisar, S. (2017). Chemistry and biosynthesis pathways of plant oleoresins: important drug sources. IJCBS, 12, 18-52.
• Sharma, A.P., & Tripathi, B. (2009). Assessment of atmospheric PAHs profile through Calotropis gigantea R. Br. leaves in the vicinity of an Indian coal-fired power plant. Environmental Monitoring and Assessment, 149(1), 477-482.
• Sharma, S., Kumari, A., & Sharma, M. (2016). Comparative GC-MS analysis of bioactive compounds in methanolic extract of Calotropis gigantea (L) WT Aiton leaf and latex. International Journal of Pharmacognosy and Phytochemical Research, 8(11), 1823-1827.
• Sivapalan, S., Dharmalingam, S., Venkatesan, V., Angappan, M., & Ashokkumar, V. (2023). Phytochemical analysis, anti-inflammatory, antioxidant activity of Calotropis gigantea and its therapeutic applications. Journal of Ethnopharmacology, 303, 115963.
• Srivastava, M.P., Awasthi, K., & Kumari, P. (2020). Antimicrobial Activity of Calotropis gigantea against Staphylococcus aureus: Eco-Friendly Management. International Journal of Plant and Environment, 6(1), 94-98.
• Tiwari, A., Singh, S., & Singh, S. (2014). Chemical analysis of leaf extracts of Calotropis procera. International Journal of Scientific and Research Publications, 4(1), 407-424.
• Wang, Z.-N., Wang, M.-Y., Mei, W.-L., Han, Z., & Dai, H.-F. (2008). A new cytotoxic pregnanone from Calotropis gigantea. Molecules, 13(12), 3033-3039.
• Yesmin, M.N., Uddin, S.N., Mubassara, S., & Akond, M.A. (2008). Antioxidant and antibacterial activities of Calotropis procera Linn. American-Eurasian Journal of Agricultural & Environmental Sciences, 4(5), 550-553.