Effect of ZnO nano priming on germination and root length of soybean seeds (Glycine max L.)

Year 2025, Volume: 12 Issue: 1, 204 - 215

Burcu Akbay , Fehime Sevil Yalçın

Abstract

Nano-priming is a pioneering method of treating seeds that improves seed germination, growth, and yield by imparting resilience to several plant stressors. Zinc oxide (ZnO) is a nanomaterial with a specific surface area, high pore volume, low toxicity, and an extended lifetime, and used in nano-priming. This study aimed to determine the effect of ZnO nanoparticles (NPs) on seed germination and root length in determining the optimum concentration of ZnO-NPs for soya plants. The transmission of electron microscopy (TEM) and zeta potential measurements were used to characterize ZnO-NPs. Soya seeds were treated with different concentrations of ZnO-NPs (0, 250, 500, 1000 and 2000 mgL-1) for 24 h. to determine the optimum concentration of ZnO-NPs for selected variants. After priming, the germination percentage and root length of each treatment were measured. The effect of ZnO nanoparticles (in soya plants was investigated by comparing them with seeds germinated in a control (hydro-priming) medium. The investigation demonstrated that the high concentration of ZnO NPs had an adverse impact on both seed germination and root length. Based on this, it was suggested that studies should be conducted including different concentrations of ZnO nanoparticles, which are thought to have a complex structure, to understand the mechanism of action, to find the appropriate concentration for soybean plants, and to increase seed germination.

Keywords

Seed priming, Metaloxide nanoparticles, Germination percentage, Root length, Glycine max L.

Ethical Statement

Canakkale Onsekiz Mart University/ Postgraduate Education Institute Ethics Committee Scientific Research Ethics Committee, E-84026528-050.01.04-2200303364.

Supporting Institution

This study is a part of Burcu AKBAY's master's thesis supported by Çanakkale Onsekiz Mart University BAP with approval date 15.05.2022.

Project Number

FYL2022-3972

Thanks

We would like to thank Assoc. Prof. Dr. Volkan Eskizeybek and Research Assistant Hakan Güven for providing the laboratory conditions for this study and Dr. Lecturer Bahri İzci for helping with statistical analyses. We would like to thank Prof. Dr Bahri İzci for his help in statistical analyses.

Effect of ZnO nano priming on germination and root length of soya (Glycine max L.) plant seeds

Abstract

Nano-priming is a pioneering method of treating seeds that improves seed germination, growth, and yield by imparting resilience to several plant stressors. Zinc oxide (ZnO) is a nanomaterial with a specific surface area, high pore volume, low toxicity, and an extended lifetime, and used in nano-priming. This study aimed to determine the effect of ZnO nanoparticles (NPs) on seed germination and root length in determining the optimum concentration of ZnO-NPs for soya plants. The transmission of electron microscopy (TEM) and zeta potential measurements were used to characterize ZnO-NPs. Soya seeds were treated with different concentrations of ZnO-NPs (0, 250, 500, 1000 and 2000 mgL-1) for 24 h. to determine the optimum concentration of ZnO-NPs for selected variants. After priming, the germination percentage and root length of each treatment were measured. The effect of ZnO nanoparticles (in soya plants was investigated by comparing them with seeds germinated in a control (hydro-priming) medium. The investigation demonstrated that the high concentration of ZnO NPs had an adverse impact on both seed germination and root length. Based on this, it was suggested that studies should be conducted including different concentrations of ZnO nanoparticles, which are thought to have a complex structure, to understand the mechanism of action, to find the appropriate concentration for soybean plants, and to increase seed germination.

Keywords

Seed priming, Metaloxide nanoparticles, Germination percentage, Root length, Glycine max L.

Ethical Statement

Canakkale Onsekiz Mart University/ Postgraduate Education Institute Ethics Committee Scientific Research Ethics Committee, E-84026528-050.01.04-2200303364.

Supporting Institution

This study is a part of Burcu AKBAY's master's thesis supported by Çanakkale Onsekiz Mart University BAP with approval date 15.05.2022.

Project Number

FYL2022-3972

Thanks

We would like to thank Assoc. Prof. Dr. Volkan Eskizeybek and Research Assistant Hakan Güven for providing the laboratory conditions for this study and Dr. Lecturer Bahri İzci for helping with statistical analyses. We would like to thank Prof. Dr Bahri İzci for his help in statistical analyses.

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