In vitro antioxidant potential of eleven medicinal herbs in Sri Lanka: Correlation with phenols and flavonoids

Year 2025, Volume: 12 Issue: 3, 630 - 644, 04.09.2025

Pathumi Ariyarathna Rizliya Visvanathan Isuri Rathnayake Terrence Madhujith Ruvini Liyanage

https://doi.org/10.21448/ijsm.1562753

Abstract

Antioxidants play a crucial role in preventing and treating non-communicable diseases (NCDs) by scavenging free radicals. Medicinal herbs, used for centuries in traditional healthcare systems, have been gaining attention recently due to the negative effects of synthetic medicines. This study assessed the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity of aqueous extracts from eleven commonly used Sri Lankan Ayurvedic plants and determined the relationship between their phenolic and flavonoid content with antioxidant activities. TPC and TFC were measured using the Folin-Ciocalteu and Aluminum chloride methods, respectively. Antioxidant activity was evaluated using ABTS, FRAP, and DPPH assays. Pearson correlation analysis assessed the relationship between TPC and TFC with antioxidant activity. Phyllanthus emblica (PE) showed the highest TPC, TFC, and antioxidant activity (p≤0.05) significantly. TPC and TFC exhibited significantly positive correlations with FRAP and ABTS assays while the DPPH assay showed a negative correlation. Phenols and flavonoids in the selected extracts may significantly contribute to the antioxidant activity measured by ABTS and FRAP assays, while other secondary metabolites and their synergism effect may influence the DPPH assay. The significant antioxidant properties of PE, highlight its potential to treat various NCDs. Further studies are essential to determine their bioactivities, effective doses, and toxicity levels.

Keywords

Medicinal plants , Antioxidant activity , Total phenolic content , Total flavonoid content

Ethical Statement

N/A

Supporting Institution

N/A

References

• Adebiyi, O.E., Olayemi, F.O., Ning-Hua, T., & Guang-Zhi, Z. (2017). In vitro antioxidant activity, total phenolic and flavonoid contents of ethanol extract of stem and leaf of Grewia carpinifolia. Beni-Suef University Journal of Basic and Applied Sciences, 6(1), 10–14. https://doi.org/10.1016/j.bjbas.2016.12.003
• Ali, S.S., Kasoju, N., Luthra, A., Singh, A., Sharanabasava, H., Sahu, A., & Bora, U. (2008). Indian medicinal herbs as sources of antioxidants. Food Research International, 41(1), 1–15. https://doi.org/10.1016/j.foodres.2007.10.001
• Amin, I., Norazaidah, Y., & Hainida, K.I.E. (2006). Antioxidant activity and phenolic content of raw and blanched Amaranthus species. Food Chemistry, 94(1), 47 52. https://doi.org/10.1016/j.foodchem.2004.10.048
• Atanassova, M., Georgieva, S., & Ivancheva, K. (2011). Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs. Journal of the University of Chemical Technology and Metallurgy, 46(1), 81–88.
• Baba, S.A., & Malik, S.A. (2015). Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah University for Science, 9(4), 449 454. https://doi.org/10.1016/j.jtusci.2014.11.001
• Bhattacharya, S.K., Bhattacharya, D., Sairam, K., & Ghosal, S. (2002). Effect of bioactive tannoid principles of Emblica officinalis on ischemia-reperfusion-induced oxidative stress in rat heart. Phytomedicine, 9(2), 171–174. https://doi.org/10.1078/0944-7113-00090
• Blainski, A., Lopes, G.C., & De Mello, J.C.P. (2013). Application and analysis of the Folin-Ciocalteu method for the determination of the total phenolic content from Limonium brasiliense L. Molecules, 18(6), 6852–6865. https://doi.org/10.3390/molecules18066852
• Brand-Williams, W., Cuvelier, M.E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25–30.
• Cai, Y., Luo, Q., Sun, M., & Corke, H. (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sciences, 74(17), 2157–2184. https://doi.org/10.1016/j.lfs.2003.09.047
• Charoenteeraboon, J., Ngamkitidechakul, C., Soonthornchareonnon, N., Jaijoy, K., & Sireeratawong, S. (2010). Antioxidant activities of the standardized water extract from fruit of Phyllanthus emblica Linn. Songklanakarin Journal of Science and Technology, 32(6), 599-604.
• Choi, C.W., Kim, S.C., Hwang, S.S., Choi, B.K., Ahn, H.J., Lee, M.Y., Park, S.H., & Kim, S.K. (2002). Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Science 163(2002), 1161-1168. https://www.elsevier.com/locate/plantsci
• Deepa, N., Kaur, C., Singh, B., & Kapoor, H.C. (2006). Antioxidant activity in some red sweet pepper cultivars. Journal of Food Composition and Analysis, 19(6–7), 572–578. https://doi.org/10.1016/j.jfca.2005.03.005
• Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P., & Vidal, N. (2006). Antioxidant activity of some Algerian medicinal plant extracts containing phenolic compounds. Food Chemistry, 97(4), 654 660. https://doi.org/10.1016/j.foodchem.2005.04.028
• Dudonné, S., Vitrac, X., Coutiére, P., Woillez, M., & Mérillon, J.M. (2009). Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. Journal of Agricultural and Food Chemistry, 57(5), 1768–1774. https://doi.org/10.1021/jf803011r
• Fadlinizal Abd Ghafar, M., Nagendra Prasad, K., Kin Weng, K., & Ismail, A. (2010). Flavonoid, hesperidine, total phenolic contents and antioxidant activities from Citrus species. African Journal of Biotechnology, 9(3), 326 330. http://www.academicjournals.org/AJB
• Fu, L., Xu, B.T., Xu, X.R., Gan, R.Y., Zhang, Y., Xia, E.Q., & Li, H.B. (2011). Antioxidant capacities and total phenolic contents of 62 fruits. Food Chemistry, 129(2), 345–350. https://doi.org/10.1016/j.foodchem.2011.04.079
• Ghasemi, K., Ghasemi, Y., & Ali Ebrahimzadeh, M. (2009). Antioxidant activity, phenol, and flavonoid contents of 13 citrus species peels and tissues. Pakistan Journal of Pharmaceutical Sciences, 22(3), 277-281.
• Gupta, A.D., Pundeer, V., Bande, G., Dhar, S., Ranganath, I.R., & Kumari, G.S. (2009). Evaluation of antioxidant activity of four folk antidiabetic medicinal plants of India. Pharmacologyonline, 1, 200–208.
• Ikram, E.H.K., Eng, K.H., Jalil, A.M.M., Ismail, A., Idris, S., Azlan, A., Nazri, H.S.M., Diton, N.A.M., & Mokhtar, R.A.M. (2009). Antioxidant capacity and total phenolic content of Malaysian underutilized fruits. Journal of Food Composition and Analysis, 22(5), 388–393. https://doi.org/10.1016/j.jfca.2009.04.001
• Krishnaraju, A.V., Rao, T.V.N., Sundararaju, D., Vanisree, M., Tsay, H.-S., & Subbaraju, G.V. (2005). Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay. International Journal of Applied Science and Engineering, 3(2), 125–134.
• Li, H.B., Wong, C.C., Cheng, K.W., & Chen, F. (2008). Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT - Food Science and Technology, 41(3), 385–390. https://doi.org/10.1016/J.LWT.2007.03.011
• Lobo, V., Chavan, A., Phatak, A., & Chandra, N. (2010). Antioxidant and free radical scavenging activity of Hygrophila schulli (Buch. -Ham.) Almeida and Almeida seeds. Advances in Bio Research, 1(2), 72-78.
• Maneetong, S. (2019). Simple extraction for the scanning of antioxidant activity of vegetables and fruits in Buriram, Thailand by DPPH, ABTS, and FRAP assays. Creative Science, 11(3), 114–121.
• Poltanov, E.A., Shikov, A.N., Dorman, H.J.D., Pozharitskaya, O.N., Makarov, V.G., Tikhonov, V.P., & Hiltunen, R. (2009). Chemical and antioxidant evaluation of Indian gooseberry (Emblica officinalis Gaertn., syn. Phyllanthus emblica L.) supplements. Phytotherapy Research, 23(9), 1309–1315. https://doi.org/10.1002/ptr
• Pozharitskaya, O.N., Ivanova, S.A., Shikov, A.N., & Makarov, V.G. (2007). Separation and evaluation of free radical-scavenging activity of phenol components of Emblica officinalis extract by using an HPTLC-DPPH• method. Journal of Separation Science, 30(9), 1250–1254. https://doi.org/10.1002/jssc.200600532
• Sadeer, N.B., Montesano, D., Albrizio, S., Zengin, G., & Mahomoodally, M.F. (2020). The versatility of antioxidant assays in food science and safety—chemistry, applications, strengths, and limitations. Antioxidants, 9(8), 1–39. https://doi.org/10.3390/antiox9080709
• Sapkota, B.K., Khadayat, K., Sharma, K., Raut, B.K., Aryal, D., Thapa, B.B., & Parajuli, N. (2022). Phytochemical analysis and antioxidant and antidiabetic activities of extracts from Bergenia ciliata, Mimosa pudica, and Phyllanthus emblica. Advances in Pharmacological and Pharmaceutical Sciences, 2022, 1–11. https://doi.org/10.1155/2022/4929824
• Saxena, M., Saxena, J., Nema, R., Singh, D., & Gupta, A. (2013). Phytochemistry of medicinal plants. Journal of Pharmacognosy and Phytochemistry, 1(6), 168-182.
• Seo, C., An, H.W., Han, W., Lee, J.W., Shrestha, K.K., Jung, W.K., Shin, J.H., & Lee, S.G. (2023). Screening of antioxidant capacity of Nepali medicinal plants with a novel singlet oxygen scavenging assay. Food Science and Biotechnology, 32(2), 221 228. https://doi.org/10.1007/s10068-022-01175-z
• Shahidi, F., & Ambigaipalan, P. (2015). Phenolics and polyphenolics in foods, beverages, and spices: Antioxidant activity and health effects—a review. Journal of Functional Foods, 18(B), 820–897. https://doi.org/10.1016/j.jff.2015.06.018
• Suryanarayana, P., Kumar, P.A., Saraswat, M., Petrash, J.M., & Reddy, G.B. (2004). Inhibition of aldose reductase by tannoid principles of Emblica officinalis: Implications for the prevention of sugar cataract. Molecular Vision, 10, 148–154.
• Tugume, P., & Nyakoojo, C. (2019). Ethno-pharmacological survey of herbal remedies used in the treatment of pediatric diseases in Buhunga parish, Rukungiri District, Uganda. BMC Complementary and Alternative Medicine, 19(353), 1-10. https://doi.org/10.1186/s12906-019-2763-6
• Tupe, R.S., Kemse, N.G., & Khaire, A.A. (2013). Evaluation of antioxidant potentials and total phenolic contents of selected Indian herbs powder extracts. International Food Research Journal, 20(3), 1053-1063.
• Ulewicz-Magulska, B., & Wesolowski, M. (2023). Antioxidant activity of medicinal herbs and spices from plants of the Lamiaceae, Apiaceae, and Asteraceae families: Chemometric interpretation of the data. Antioxidants, 12(2039), 1 19. https://doi.org/10.3390/antiox12122039
• Verma, A.R., Vijayakumar, M., Mathela, C.S., & Rao, C.V. (2009). In vitro and in vivo antioxidant properties of different fractions of Moringa oleifera leaves. Food and Chemical Toxicology, 47(9), 2196–2201. https://doi.org/10.1016/j.fct.2009.06.005
• World Health Organization. (2023). Non-communicable diseases. World Health Organization. https://www.who.int/news-room/fact-sheets/detail/noncommunicable-diseases