Phytochemical analysis and antioxidant evaluation of methanolic extracts from Lepidium sativum seeds and Averrhoa carambola fruits: A comparative analysis

Year 2025, Volume: 12 Issue: 3, 604 - 614, 04.09.2025

Suchita Gupta , Reena Gupta

https://doi.org/10.21448/ijsm.1574497

Abstract

This study compares the antioxidant potential and phytochemical composition of methanolic extracts from Lepidium sativum (seeds) and Averrhoa carambola (fruits), chosen due to their traditional medicinal uses and differing plant parts, to explore their potential health benefits. The total phenolic content (TPC), total flavonoid content, and various in vitro antioxidant assays—including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, hydrogen peroxide scavenging, reducing power, superoxide dismutase (SOD) scavenger, and ferric thiocyanate tests—were evaluated. The results revealed that Lepidium sativum exhibited significantly higher TPC (270.34 mg/gm) and flavonoid content (85.05 mg/gm) compared to Averrhoa carambola (TPC: 49.04 mg/gm, flavonoids: 21.10 mg/gm). Additionally, Lepidium sativum consistently showed superior antioxidant efficacy across all assays, with higher inhibition percentages and lower inhibitory concentration 50 (IC50) values for DPPH, hydrogen peroxide, and superoxide dismutase scavenging. The pronounced antioxidant activity of Lepidium sativum, likely due to its enriched phenolic and flavonoid content, highlights its potential as a natural remedy for managing oxidative stress-related diseases and warrants further investigation into its therapeutic applications for oxidative damage management.

Keywords

Oxidative stress , Antioxidants , Lepidium sativum , Averrhoa carambola , Hydrogen peroxide , Oxidative damage

References

• Ahsan, S.K., Tariq, M., Ageel, M., Alyahya, M.A., & Shah, A.H. (1989). Studies on some herbal drugs used in fracture healing. International Journal of Crude Drug Research, 27(4), 235-239. https://doi.org/10.3109/13880208909116909
• Alara, O.R., Abdurahman, N.H., Ukaegbu, C.I., & Kabbashi, N.A. (2019). Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques. Journal of Taibah University for Science, 13(1), 414-422. https://doi.org/10.1080/16583655.2019.1582460
• Ali, M.S., Amin, MR., Kamal, CM., & Hossain, M.A. (2013). In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the A. philippense L. leaves. Asian Pacific Journal of Tropical Biomedicine, 3(6), 464-469. https://doi.org/10.1016/S2221-1691(13)60097-0
• Arika, W., Kibiti, C.M., Njagi, J.M., & Ngugi, M.P. (2019). In vitro antioxidant properties of dichloromethanolic leaf extract of Gnidia glauca (Fresen) as a promising antiobesity drug. Journal of Evidence Based Integrative Medicine, 2019, 24. https://doi.org/10.1177/2515690X19883258
• Bhat, A.H., Dar, K.B., Anees, S., Zargar, M.A., Masood, A., Sofi, M.A., & Ganie, S.A. (2015). Oxidative stress, mitochondrial dysfunction and neurodegenerative diseases; a mechanistic insight. Biomedicine & Pharmacotherapy, 74, 101 110. https://doi.org/10.1016/j.biopha.2015.07.025
• Chatoui, K., Harhar, H., El, K.T., & Tabyaoui, M. (2020). Chemical composition and antioxidant capacity of Lepidium sativum seeds from four regions of Morocco. Evidence-Based Complementary and Alternative Medicine, 2020(1), 7302727. https://doi.org/10.1155/2020/7302727
• Choi, C.W., Kim, S.C., Hwang, S.S., Choi, B.K., Ahn, H.J., Lee, M.Y., Park, S.H., & Kim, S.K. (2002). Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Science, 163(6), 1161-1168. https://doi.org/10.1016/S0168-9452(02)00332-1
• Chopra, R.N., Nayar, S.L., & Chopra, I.C. (1992). Glossary of Indian medicinal plants. Council of Scientific & Industrial Research.
• Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P., & Vidal, N. (2006). Antioxidant activity of some Algerian medicinal plant’s extracts containing phenolic compounds. Food Chemistry, 97(4), 654 660. https://doi.org/10.1016/j.foodchem.2005.04.028
• Eddouks, M., Maghrani, M., Zeggwagh, N.A., & Michel, J.B. (2005). Study of the hypoglycaemic activity of Lepidium sativum L. aqueous extract in normal and diabetic rats. Journal of Ethnopharmacology, 97(2), 391-395. https://doi.org/10.1016/j.jep.2004.11.030.
• Ghaima, K.K., Hashim, N.M., & Ali, S.A .(2013). Antibacterial and antioxidant activities of ethyl acetate extract of nettle (Urtica dioica) and dandelion (Taraxacum officinale). Journal of Applied Pharmaceutical Science, 3(5), 96-99. https://doi.org/10.7324/JAPS.2013.3518
• Goyal, M.R., & Suleria, H.A. (2019). Human health benefits of plant bioactive compounds: potentials and prospects. CRC Press.
• Harborne, A.J. (1998). Phytochemical methods a guide to modern techniques of plant analysis. springer science & business media.
• Kokate, C.K. (2006). Preliminary phytochemical analysis. Practical Pharmacognosy. New Delhi: Vallabh Prakashan, 1, 111.
• Knekt, P., Kumpulainen, J., Järvinen, R., Rissanen, H., Heliövaara, M., Reunanen, A., Hakulinen, T., & Aromaa, A. (2002). Flavonoid intake and risk of chronic diseases. American Journal of Clinical Nutrition, 76(3), 560 568. https://doi.org/10.1093/ajcn/76.3.560
• Malar, J., Chairman, K., Singh, A.R., Vanmathi, J.S., Balasubramanian, A., & Asanthi, K. (2014). Antioxidative activity of different parts of the plant Lepidium sativum Linn. Biotechnology Reports, 3, 95-98. https://doi.org/10.1016/j.btre.2014.05.006
• Manda, H., Vyas, K., Pandya, A., & Singhal, G. (2012). A complete review on: Averrhoa carambola. World Journal of Pharmacy and Pharmaceutical Sciences, 1(1), 17-33.
• Miller, A.L. (1996). Antioxidant flavonoids: structure, function and clinical usage. Alternative Medicine Review, 1(2), 103-111.
• Moriasi, G.A., Ireri, A.M., & Ngugi, M.P. (2020). In vivo cognitive‐enhancing, ex vivo malondialdehyde‐lowering activities and phytochemical profiles of aqueous and methanolic stem bark extracts of Piliostigma thonningii (Schum.). International Journal of Alzheimer's Disease, 2020(1), 1367075. https://doi.org/10.1155/2020/1367075
• Mwihia, S.K. (2017). In vitro antibacterial and antioxidant activities of methanolic and dichloromethanolic seed extracts of Kenyan Annona squamosa Linn. Kenyatta University: Nairobi, Kenya. https://doi.org/10.13140/RG.2.2.22179.40489
• Nadkarni, A.K. (2007). Dr. KM Nadkarni's Indian materia medica: with Ayurvedic, Unani-tibbi, Siddha, allopathic, homeopathic, naturopathic & home remedies, appendices & indexes. Popular Prakashan.
• Ndhlala, A.R., Moyo, M., Van, & Staden J. (2001). Natural antioxidants: fascinating or mythical biomolecules. Molecules, 15(10), 6905 30. https://doi.org/10.3390/molecules15106905
• Nishikimi, M., Rao, N.A., & Yagi, K. (1972). The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochemical and Biophysical Research Communications, 46(2), 849-54. https://doi.org/10.1016/S0006-291X(72)80218-3
• Ojiewo, C., Tenkouano, A., d’A. Hughes, J., & Keating, J.D.H. (2013). Diversifying diets: Using indigenous vegetables to improve profitability, nutrition and health in Africa. In J. Fanzo, D. Hunter, T. Borelli, & F. Mattei (Eds.), Diversifying food and diets: Using agricultural biodiversity to improve nutrition and health (pp. 291-302). Routledge. https://doi.org/10.4324/9780203127261
• Panche, A.N., Diwan, A.D., & Chandra, S.R. (2016). Flavonoids: an overview. Journal of Nutritional Science, 5, 47. https://doi.org/10.1017/jns.2016.41
• Paranjape, A.N., & Mehta, A.A. (2006). Study on clinical efficacy of Lepidium sativum seeds in treatment of bronchial asthma . Iranian Journal of Pharmacology and Therapeutics, 5, 55-59.
• Patel, M.V., Patel, K.B., Gupta, S., Michalsen, A., Stapelfeldt, E. & Kessler, C.S. (2015). A complex multiherbal regimen based on Ayurveda medicine for the management of hepatic cirrhosis complicated by ascites: nonrandomized, uncontrolled, single group, open‐label observational clinical study. Evidence-based Complementary and Alternative Medicine, 2015(1), 613182. https://doi.org/10.1155/2015/613182
• Patil, A.G., Patil, D.A., Phatak, A.V., & Chandra, N. (2010). Physical and chemical characteristics of carambola (Averrhoa carambola L.) fruit at three stages of maturity. International Journal of Applied Biology and Pharmaceutical Technology, 1(2), 624-629.
• Pietta, P.G. (2000). Flavonoids as antioxidants. Journal of Natural Products, 63(7), 1035-42. https://doi.org/10.1021/np9904509
• Quettier-Deleu, C., Gressier, B., Vasseur, J., Dine, T., Brunet, C., Luyckx, M., Cazin, M., Cazin, J.C., Bailleul, F., & Trotin, F. (2000). Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. Journal of Ethnopharmacology, 72(1-2), 35-42. https://doi.org/10.1016/S0378-8741(00)00196-3
• Rajashekar, C.B., Carey, E.E., Zhao, X., & Oh, M.M. (2009). Health-promoting phytochemicals in fruits and vegetables: Impact of abiotic stresses and crop production practices. Functional Plant Science and Biotechnology, 3(1), 30-38.
• Ruch, RJ., Cheng, SJ., & Klaunig, J.E. (1989). Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 10(6), 1003-8. https://doi.org/10.1093/carcin/10.6.1003
• Sharief, M., & Gani, Z.H. (2004). Garden cress (Lepidium sativum) seeds as oral contraceptive plant in mice. Saudi Med. J., 25(7), 965-966.
• Shirwaikar, A., Patel, B., Kamariya, Y., Parmar, V., & Khan, S. (2011). In vitro free radical scavenging potential of defatted ethanolic extract of the seeds of Lepidium sativum Linn. Chinese Journal of Natural Medicines, 9(6), 435 40. https://doi.org/10.3724/SP.J.1009.2011.00435
• Tan, B.L., Norhaizan, M.E., Liew, W.P., & Sulaiman Rahman, H. (2018). Antioxidant and oxidative stress: a mutual interplay in age-related diseases. Frontiers in Pharmacology, 9, 1162. https://doi.org/10.3389/fphar.2018.01162
• Ulusu, F.( 2023). Zinc fertilizer applications to Ocimum basilicum L. under water stress: Changes in the total phenolic and flavonoid content, essential oil compounds and morphological properties. Journal of Production Agriculture, 4(2), 117 27. https://doi.org/10.56430/japro.1340577
• Ulusu, F.(2024). Effects of fertilizer type on phenolic compounds, essential oil content, and biological activities of Coriandrum sativum L. Anatolian journal of botany, 8(2), 106-13. https://doi.org/10.30616/ajb.1455761
• Ulusu, F., Sarilmaz, A., Ulusu, Y., & Ozel, F. (2024). Quaternary nanorods: promising versatile agents for cancer therapy, antimicrobial strategies and free radical neutralization. Particulate Science and Technology, 42(6), 944-52. https://doi.org/10.1080/02726351.2023.2299869
• Vertuani, S., Angusti, A., & Manfredini. S. (2004). The antioxidants and pro-antioxidants network: an overview. Current Pharmaceutical Design, 10(14), 1677 94. https://doi.org/10.2174/1381612043384655
• Wang, X.Q., Zou, X.R., & Zhang, Y.C. (2016). From “kidneys govern bones” to chronic kidney disease, diabetes mellitus, and metabolic bone disorder: a crosstalk between traditional Chinese medicine and modern science. Evidence-Based Complementary and Alternative Medicine, 2016(1), 4370263. https://doi.org/10.1155/2016/4370263
• Warrier, P.K. (1993). Indian medicinal plants: a compendium of 500 species. Orient Blackswan.
• Wright, C.I., Van-Buren, L., Kroner, CI., & Koning, M.M. (2007). Herbal medicines as diuretics: a review of the scientific evidence. Journal of Ethnopharmacology, 114(1), 1-31. https://doi.org/10.1016/j.jep.2007.07.023
• Yasmin, N., & Kaushal, S.O. (2023). In vitro Evaluation of Antioxidant Activity of Ripe Fruits of Averrhoa carambola L. Current Trends in Biotechnology and Pharmacy, 17(1), 688-696. https://doi.org/10.5530/ctbp.2023.1.10