First report on determination of bioelement, vitamin content and antioxidant properties of Verbascum golawanense

Year 2025, Volume: 12 Issue: 3, 582 - 592, 04.09.2025

Ahmet Bakır , Suat Ekin , Mehmet Fırat

https://doi.org/10.21448/ijsm.1584793

Abstract

This study was designed to investigate the antioxidant, antiradical potential and element and vitamin (A, E, K and C) levels in leaf extracts of Verbascum golawanense belonging to Scrophulariaceae family. For this purpose, DPPH, ABTS, anti-haemolytic activity, total flavonoid, phenolic content, total antioxidant capacity and vitamin C content were determined and measured spectrophotometrically. Vitamin A, E and K were analysed by HPLC and elemental analysis was performed by ICP-OES method. Phenolic, flavonoid and antioxidant capacity of the plant were determined as 5.861 ± 0.212 mg GA/g, 26.422 ± 0.613 mg QE/g and 124.359 ± 2.562 mM AA/g, respectively. K, Na, Fe, V, retinol, α-tocopherol, phylloquinone and vitamin C were determined as 3.086 ± 0.0131 mmol/kg, 2.260 ± 0.0266 mmol/kg, 1.1465 ± 0.0731 mmol/kg, 29.326 ± 3.072 µmol/kg, 0.1107 ± 0.013 µmol/kg, 0.4002 ± 0.16 µmol/kg, 1.101 ± 0.118 µmol/kg and 2552.0126 ± 187.056 mg/100g, respectively. The IC50 values related to the in vitro antioxidant properties of DPPH, ABTS and anti-haemolytic activity assays were 19.170 ± 0.615 μg/mL, 26.877 ± 0.461 μg/mL and 64.87 ± 2.24 μg/mL. In vitro studies revealed that the plant has a good antioxidant potential. It was determined that the vitamin and element content was high and total phenol, flavonoid and antioxidant potential were at reasonable levels. ABTS and DPPH tests showed a promising antioxidant power. These results indicated that the extract could be a potential alternative and could be investigated for the discovery and development of new chemical compounds for the treatment of various diseases.

Keywords

Verbascum golawanense , Antioxidant activity , Methanol extract , Bioelement , Vitamin

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