Antimicrobial activity of Patchouli essential oil (Pogostemon cablin B.) against skin microbiome

Year 2025, Volume: 12 Issue: 4, 820 - 831

Aqila Hafizha Hanun , Miftahul Silvani Andra Wahdini Elin Julianti Afrillia Nuryanti Garmana Muhamad İnsanu

https://doi.org/10.21448/ijsm.1485651

Abstract

: Patchouli Essential Oil (PEO) is derived from the leaves of the patchouli plant (Pogostemon cablin) and is renowned for its distinct fragrance and therapeutic properties. PEO exhibits potent antibacterial activity against various skin pathogens implicated in acne, dermatitis, dandruff, and other skin infections. This study aims to standardize and determine the potential of PEO to inhibit bacteria and fungi that cause skin problems. Standardization of patchouli essential oil (PEO) by the requirements of the Indonesian National Standards (SNI) for patchouli oil (06-2385-2006) shows that seven test characteristics meet the requirements, including organoleptic, specific gravity, solubility in ethanol, acid number, ester number, optical rotation, and patchouli alcohol content. The antibacterial and antifungal activity test was carried out qualitatively by measuring the diameters of the inhibition zones using the agar disc diffusion method, quantitatively by determining the value of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) or minimum fungicidal concentration (MFC) using the broth microdilution method and agar diffusion method, respectively. PEO has strong antibacterial and antifungal activity against Propionibacterium acnes ATCC 11827, Staphylococcus aureus ATCC 6538, Staphylococcous epidermidis ATCC 1228, and Malassezia furfur ATCC 14521 with MIC–MBC values of 312.5 μg/mL, 156.25–312.5 μg/mL, 187.5–375 μg/mL, and 900–>1000 μg/mL respectively. Based on the TLC-Bioautography test, compounds with antibacterial activity are considered sesquiterpenes.

Keywords

Antibacterial , Antifungal , Patchouli , Essential oil , Skin microbiome

References

• Abram, P.H., Putri, W., Nuryanti, S., & Sabang, S.M. (2021). Characterization of patchouli oil (Pogostemon cablin Benth) production of Tinombala Village, Ongka Malino District, Parigi Moutong Regency. Journal of Physics: Conference Series, 2126(1). https://doi.org/10.1088/1742-6596/2126/1/012024
• Aisyah, Y., Anwar, S.H., & Annisa, Y. (2020). Effect of the Fractional Distillation on an Increment Patchouli Alcohol Content in Patchouli Oil. Iceo 2019, 80 85. https://doi.org/10.5220/0009957100800085
• Carmona-Cruz, S., Orozco-Covarrubias, L., & Sáez-de-Ocariz, M. (2022). The human skin microbiome in selected cutaneous diseases. Frontiers in Cellular and Infection Microbiology, 12(March), 1–9. https://doi.org/10.3389/fcimb.2022.834135
• Chiller, K., Selkin, B.A., & Murakawa, G.J. (2001). Skin microflora and bacterial infections of the skin. Journal of Investigative Dermatology Symposium Proceedings, 6(3), 170–174. https://doi.org/10.1046/j.0022-202x.2001.00043.x
• Das, K. (2015). Patchouli (Pogostemon Cablin Benth) Oils. In Essential Oils in Food Preservation, Flavor and Safety. Elsevier Inc. https://doi.org/10.1016/B978-0-12-416641-7.00072-9
• Donelian, A., Carlson, L.H.C., Lopes, T.J., & Machado, R.A.F. (2009). Comparison of extraction of patchouli (Pogostemon cablin) essential oil with supercritical CO2 and by steam distillation. Journal of Supercritical Fluids, 48(1), 15 20. https://doi.org/10.1016/j.supflu.2008.09.020
• Eisenstein, M. (2020). The skin microbiome. Nature, 588(7838), S209. https://doi.org/10.1038/d41586-020-03523-7
• Handayani, R., A, S.R., & Gumilar, I. (2015). Karakteristik Fisiko-Kimia Minyak Biji Bintaro (Cerbera manghas L) dan Potensinya sebagai Bahan Baku Pembuatan Biodiesel. Jurnal Akuatika, VI(2), 177–186. http://jurnal.unpad.ac.id/akuatika/article/viewFile/7479/3436
• Junren, C., Xiaofang, X., Mengting, L., Qiuyun, X., Gangmin, L., Huiqiong, Z., Guanru, C., Xin, X., Yanpeng, Y., Fu, P., & Cheng, P. (2021). Pharmacological activities and mechanisms of action of Pogostemon cablin Benth: A review. Chinese Medicine (United Kingdom), 16(1), 1–20. https://doi.org/10.1186/s13020-020-00413-y
• Kronstrand, R., & Jones, A.W. (2000). Drugs of Abuse | Analysis. Encyclopedia of Forensic Sciences, 598–610. https://doi.org/10.1006/rwfs.2000.0494
• Kühbacher, A., Burger-Kentischer, A., & Rupp, S. (2017). Interaction of candida species with the skin. Microorganisms, 5(2), 1–12. https://doi.org/10.3390/microorganisms5020032
• Kuspradini, H., Sinta, S.S., & Putri, A.S. (2021). Karakteristik Minyak Atsiri dari Tumbuhan Aromatik Hutan Tropis Jenis Litsea spp dan Potensinya sebagai Antimikroba. Minyak Atsiri: Produksi Dan Aplikasinya Untuk Kesehatan, 50–84.
• Leung, B.K.F. (2018). The Introduction to Microbiology. Christianity in Modern China. https://doi.org/10.1007/978-981-10-6665-8_1
• Lubis, A., Mandang, T., Hermawan, W., & Sutrisno. (2022). Characterization of the yield and quality of Patchouli oil based on the size of chopping and drying type. IOP Conference Series: Earth and Environmental Science, 1038(1), 0–10. https://doi.org/10.1088/1755-1315/1038/1/012075
• Maurya, A., Prasad, J., Das, S., & Dwivedy, A.K. (2021). Essential oils and their application in food safety. Frontiers in Sustainable Food Systems, 5(May). https://doi.org/10.3389/fsufs.2021.653420
• Saunte, D.M.L., Gaitanis, G., & Hay, R.J. (2020). Malassezia-Associated Skin Diseases, the Use of Diagnostics and Treatment. Frontiers in Cellular and Infection Microbiology, 10(March), 1–11. https://doi.org/10.3389/fcimb.2020.00112
• Schommer, N.N., & Gallo, R.L. (2013). Structure and function of the human skin microbiome. Trends in microbiology, 21(12), 660–668. https://doi.org/10.1016/j.tim.2013.10.001
• Srivastava, S., Lal, R.K., Singh, V.R., Rout, P.K., Padalia, R.C., Yadav, A.K., Bawitlung, L., Bhatt, D., Maurya, A.K., Pal, A., Bawankule, D.U., Mishra, A., Gupta, P., & Chanotiya, C. S. (2022). Chemical investigation and biological activities of Patchouli (Pogostemon cablin (Blanco) Benth) essential oil. Industrial Crops and Products, 188(PA), 115504. https://doi.org/10.1016/j.indcrop.2022.115504
• Taguri, T., Tanaka, T., & Kouno, I. (2006). Antibacterial spectrum of plant polyphenols and extracts depending upon hydroxyphenyl structure. Biological and Pharmaceutical Bulletin, 29(11), 2226–2235. https://doi.org/10.1248/bpb.29.2226
• Taylor, T.A., & Unakal, C.G. (2023). Staphylococcus aureus Infection. StatPearls Publishing LLC. https://www.ncbi.nlm.nih.gov/books/NBK441868/#article-29453.s14
• Vijaya Chandra, S.H., Srinivas, R., Dawson, T.L., & Common, J.E. (2021). Cutaneous Malassezia: Commensal, Pathogen, or Protector? Frontiers in Cellular and Infection Microbiology, 10(January), 1–16. https://doi.org/10.3389/fcimb.2020.614446
• Wei, A., & Shibamoto, T. (2007). Antioxidant activities and volatile constituents of various essential oils. Journal of Agricultural and Food Chemistry, 55(5), 1737 1742. https://doi.org/10.1021/jf062959x
• Xian, Y.F., Li, Y.C., Ip, S.P., Lin, Z.X., Lai, X.P., & Su, Z.R. (2011). Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages. Experimental and Therapeutic Medicine, 2(3), 545 550. https://doi.org/10.3892/etm.2011.233