HRLC-MS analysis of methanolic leaf extract of Morus macroura Miq.

Year 2025, Volume: 12 Issue: 4, 755 - 762

Priyanka Sutar , Dattatraya K. Gaikwad

Abstract

The present study aimed to analyze the key chemical constituents of the methanolic leaf extract of Morus macroura using high-resolution liquid chromatography-mass spectrometry (HR-LCMS). Commonly known as king white mulberry, M. macroura is a medicinal herb traditionally used to treat various ailments. The HR-LCMS analysis revealed the presence of organic compounds, including amino acids and phenols, in both positive and negative electrospray ionization (ESI) chromatograms. Notable compounds identified in the positive ESI chromatogram included m-coumaric acid, gibberellin A-105, neomethymycin, and iproniazide. Similarly, the negative ESI chromatogram detected compounds such as 1,25-dihydroxy vitamin D3-26,23-lactone, alangimarckine, tyrosyl-isoleucine, hypoglycin B, tubulosine, and 2-phenylaminoadenosine. The presence of these bioactive compounds suggests that M. macroura leaf extract holds potential for use in future pharmaceutical applications.

Keywords

Moraceae , Morus macroura , HRLC-MS , methanolic extract , phytochemicals

Thanks

To The Head, Department of Botany, Shivaji University, Kolhapur for providing facilities during the work.

References

• Budiman, A., Aulifa, D.L., Kusuma, A.S., & Sulastri, A. (2017). Antibacterial and antioxidant activity of black mulberry (Morus nigra L.) extract for acne treatment. Pharmacognosy Journal, 9(5), 611-614.
• Byung, H.K., Eun, H.Y., & Yu, C.L. (2019). Anticancer activity of Tubulosine through suppression of Interleukin-6- Induced Janus kinase 2/Signal Transducer and Activation of Transcription 3 signaling. Journal of Breast Cancer, 22(3), 362-374.
• Das, B.C., & Krishnaswami (1965). Some observations on interspecific hybridization in mulberry. Indian J. Sericulture, 4, 1-8.
• Duke, J.A., & Wain, K.K. (1981). Medicinal Plants of the World. Computer Index with More than 85,000 Entries, 3 Vols.
• Entzeroth, M., & Ratty, A. (2017). Monoamine oxidase inhibitors—revisiting a therapeutic principle. Open Journal of Depression, 631-68. https://doi.org/10.4236/ojd.2017.62004
• Farrag, E.K., Kassem, M.E.S., Bayoumi, D., Shake, S.E., Afifi, M.S. (2017). Phytochemical study, phenolic profile and antigastric ulcer activity of Morus macroura Miq. fruits extract. J App Pharm Sci., 7(05) 152-160. https://doi.org/10.7324/JAPS.2017.70527
• Hăbeanu, M., Gheorghe, A., & Mihalcea, T. (2023). Silkworm Bombyx mori—sustainability and economic opportunity, particularly for Romania. Agriculture, 13(6), 1209. https://doi.org/10.3390/agriculture13061209
• Hardart, G.E., Sullivan, G.W., Carper, H.T., & Mandell, G.L., (1991). Adenosine and 2-phenyl aminoadenosine (cv-1808) inhiboit human neutropnil bactericidal function. Infection and Immunity, 59(3), 885-889.
• Jasmansyah, Hakim, E.H., & Syah, Y.M. (2019). Antibacterial constituents from Morus macroura. Res. J. Chem. Environ., 23(11), 37-40.
• Jena, L., Waghmare, P., Kashikar, S., Kumar, S., & Harinath, B.C., (2014). Compulational approach to understanding the mechanism of action of isoniazid, an anti-TB drug. International Journal of Mycobacteriology, 3, 276-282.
• Lei, B., Wei, C.J., & Tu, S.C. (2000). Action mechanism of antitubercular isoniazid. Activation by mycobacterium tuberculosis Kat G, isolation, and characterization of inha inhibitor. Journal of Biology and chemistry, 275, 2520-2526.
• Liang, Y., Quenelle, D, Vogel, J.L., Mascaro, C., Ortega, A., & Kristie, T.M. (2013). A novel selective LSD1/KDM1A inhibitor epigenetically blocks herpes simplex virus lytic replication and reactivation from latency. mBio. 5, 4(1), e00558 12. https://doi.org/10.1128/mBio.00558-12
• Macedo, D., Filho, J., Sousa, J.M.C., Nadia, C., Soares de., Quevedo, J., Barichello, T., Junior, H.V.N. (2017). Antidepressant, antimicrobial or both? Gut microbiota dysbiosis in depression and possible implications of the antimicrobial effects of antidepressant drugs for antidepressant effectiveness. Journal of Affective Disorders, 208, 22-32.
• Vijayan, K. (2009). Approaches for enhancing salt tolerance in mulberry (Morus L.) A review Plant Omics J, 2(1), 41-59.
• Wu, H., Sun, N., Lou, D., Geng, T., Lu, F., Li, Y., Wang, S., & Zhu, G. (2025). Unveiling the unique aroma of Morus macroura through integrated volatile metabolome and transcriptome analysis, Scientia Horticulturae, 339, 113814. https://doi.org/10.1016/j.scienta.2024.113814
• Yang, C.X., Liu, S.Y., Zerega, N.J.C., Stull, G.W., Gardner, E.M., Tian, Q., Gu, W., Lu, Q., Folk, R.A., Kates, H.R., Guralnick, R.P., Soltis, D.E., Soltis, P.S., Wang, Y.H., & Yi, T.S. (2023). Phylogeny and biogeography of Morus (Moraceae). Agronomy 13(8), 2021. https://doi.org/10.3390/agronomy13082021