Experimental Investigations on the Antioxidant, Anti-Quorum Sensing, and Antibiofilm Activities of Ethanolic Extracts from Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys, and Thymus longicaulis subsp. longicaulis

Year 2025, Volume: 6 Issue: 2, 102 - 110, 27.08.2025

Ayşegül Akkoyunlu , Gorkem Dulger , Bahar Gedik , Başaran Dülger

https://doi.org/10.53811/ijtcmr.1683587

Abstract

Objective: The aim of this study was to evaluate the oxidant and antioxidant capacities, anti-quorum detection (QS) and antibiofilm activities of ethanol extracts obtained from Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys and Thymus longicaulis subsp. longicaulis.
Materials and Methods: Antibiofilm activities were evaluated using the microplate method against a biofilm-forming Escherichia coli reporter strain. Anti-QS activities were evaluated qualitatively using the well diffusion method with Chromobacterium violaceum ATCC 12472 as a biosensor. Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were measured using commercial kits.
Results: All three plant extracts showed strong biofilm inhibition. T. chamaedrys subsp. chamaedrys showed the highest antibiofilm activity with 94.07% inhibition at 12.5 µg/mL. In the anti-QS screening, T. longicaulis subsp. longicaulis extract showed the largest violacein inhibition zone (21.67±3.51 mm) at 75 mg/mL. This extract also exhibited the most favorable oxidative profile with the highest TAS and lowest OSI values among the tested samples.
Conclusion: Ethanol extracts of the tested medicinal plants, especially T. longicaulis subsp. longicaulis, have significant antibiofilm, anti-QS and antioxidant activities. The findings indicate the potential of the extracts as natural agent sources to control the virulence properties of pathogens.

Keywords

biofilm , Chromobacterium violaceum ATCC 12472 , oxidative stres index

Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys ve Thymus longicaulis subsp. longicaulis'ten Elde Edilen Etanolik Ekstraktların Antioksidan, Anti-Quorum Algılama ve Antibiyofilm Aktiviteleri Üzerine Deneysel Araştırmalar

Abstract

Amaç: Bu çalışmanın amacı Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys ve Thymus longicaulis subsp. longicaulis'ten elde edilen etanol özütlerinin oksidan ve antioksidan kapasitelerini, anti-quorum tespiti (QS) ve antibiyofilm aktivitelerini değerlendirmektir.
Gereçler ve Yöntemler: Antibiyofilm aktiviteleri, biyofilm oluşturan bir Escherichia coli raportör suşuna karşı mikro plaka yöntemi kullanılarak değerlendirildi. Anti-QS aktiviteleri, biyosensör olarak Chromobacterium violaceum ATCC 12472 ile kuyu difüzyon yöntemi kullanılarak nitel olarak değerlendirildi. Toplam antioksidan durumu (TAS), toplam oksidan durumu (TOS) ve oksidatif stres indeksi (OSI) ticari kitler kullanılarak ölçüldü.
Sonuçlar: Üç bitki özütü de güçlü biyofilm inhibisyonu gösterdi. T. chamaedrys subsp. chamaedrys, 12,5 µg/mL'de %94,07 inhibisyonla en yüksek antibiyofilm aktivitesini gösterdi. Anti-QS taramasında, T. longicaulis subsp. longicaulis özütü, 75 mg/mL'de en büyük violacein inhibisyon bölgesini (21,67±3,51 mm) gösterdi. Bu özüt ayrıca test edilen örnekler arasında en yüksek TAS ve en düşük OSI değerleriyle en uygun oksidatif profili sergiledi.
Sonuç: Test edilen tıbbi bitkilerin, özellikle T. longicaulis subsp. longicaulis'in etanol özütleri, önemli antibiyofilm, anti-QS ve antioksidan aktivitelere sahiptir. Bulgular, özütlerin patojenlerin virülans özelliklerini kontrol etmek için doğal ajan kaynakları olarak potansiyelini göstermektedir.

Keywords

Biyofilm , Chromobacterium violaceum ATCC 12472 , Oksidatif stres indeksi

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