The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season

Ayhan Ata; Muhammed Enes Inanc; Sukru Gungor

The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season

Abstract

Bu çalışmanın amacı, sezon dışı dönemde değişik oranlardaki α-tokoferolün koç spermasının dondurulabilirliğine etkisini incelemektir. Çalışmada 4 adet Merinos koçu (2- 3 yaşlarında ve Bademli/Burdur’da özel bir işletmede) kullanıldı. Elektroejakülatör kullanılarak koçlardan alınan ejakulatlardan > %80 motilite ve 1.5 x109 spermatozoa/ml üzerinde yoğunluğu bulunan ejakulatlar birleştirildi. Birleştirilen ejakulat beş eşit gruba ayrılarak 0 mg (kontrol), 100 µM, 200 µM, 400µM or 800 µM α-tokoferol içeren tris ile sulandırıldı, 4 C⁰’de ekibrilasyon yapılarak mini payetlerde donduruldu ve sıvı azotta depo edildi. Payetler daha sonda spermatolojik analizler için 37 C⁰’de 30 sn çözdürüldü. Kontrol grubuna (37.50±7.90; 26.85±3.17) göre sırası ile en yüksek motilite ve membran bütünlüğü 100 µM grupta (47.77±4.40; 34.83±2.64) tespit edildi (p<0.05). Ayrıca gruplar arası anormal sperma muayene sonuçlarında istatistiksel bir farklılık bulunmadı (p>0.05). Sonuç olarak, 100 µM α-tokoferol koç sperma dondurma sulandırıcılarına kontrol grubu ile karşılaştırıldığında sezon dışında motilite ve membran bütünlüğünü iyileştirdiği için eklenmelidir.

Keywords

α-tokoferol,koç sperması dondurulabilirliliği,sezon dışı,motilite,membran bütünlüğü,anormal spermatozoa oranı

References

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Details

Primary Language

English

Subjects

Veterinary Surgery

Journal Section

Research Article

Authors

Ayhan Ata This is me

Muhammed Enes Inanc

Sukru Gungor This is me

Publication Date

December 22, 2018

Submission Date

April 1, 2018

Acceptance Date

August 1, 2018

Published in Issue

Year 2018 Volume: 1 Number: 2

IZ

https://izlik.org/JA48EL78KD

Cite

RIS / Bibtex

APA

Ata, A., Inanc, M. E., & Gungor, S. (2018). The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season. International Journal of Veterinary and Animal Research, 1(2), 44-46. https://izlik.org/JA48EL78KD

AMA

1.Ata A, Inanc ME, Gungor S. The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season. International Journal of Veterinary and Animal Research. 2018;1(2):44-46. https://izlik.org/JA48EL78KD

Chicago

Ata, Ayhan, Muhammed Enes Inanc, and Sukru Gungor. 2018. “The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season”. International Journal of Veterinary and Animal Research 1 (2): 44-46. https://izlik.org/JA48EL78KD.

EndNote

Ata A, Inanc ME, Gungor S (December 1, 2018) The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season. International Journal of Veterinary and Animal Research 1 2 44–46.

IEEE

[1]A. Ata, M. E. Inanc, and S. Gungor, “The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season”, International Journal of Veterinary and Animal Research, vol. 1, no. 2, pp. 44–46, Dec. 2018, [Online]. Available: https://izlik.org/JA48EL78KD

ISNAD

Ata, Ayhan - Inanc, Muhammed Enes - Gungor, Sukru. “The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season”. International Journal of Veterinary and Animal Research 1/2 (December 1, 2018): 44-46. https://izlik.org/JA48EL78KD.

JAMA

1.Ata A, Inanc ME, Gungor S. The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season. International Journal of Veterinary and Animal Research. 2018;1:44–46.

MLA

Ata, Ayhan, et al. “The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season”. International Journal of Veterinary and Animal Research, vol. 1, no. 2, Dec. 2018, pp. 44-46, https://izlik.org/JA48EL78KD.

Vancouver

1.Ayhan Ata, Muhammed Enes Inanc, Sukru Gungor. The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season. International Journal of Veterinary and Animal Research [Internet]. 2018 Dec. 1;1(2):44-6. Available from: https://izlik.org/JA48EL78KD