SWAP-70 Antikoru Pozitif Multipl Skleroz Serum Antikorlarının İntraventriküler Uygulamasının Motor Aktivite ve Beyin Histolojisine Etkileri

Year 2018, Volume: 8 Issue: 1, 7 - 10, 30.04.2018

Recai Türkoğlu , Canan Ulusoy Vuslat Yılmaz

https://doi.org/10.26650/experimed.2018.377256

Abstract

DOI:
10.26650/experimed.2018.377256

Amaç: SWAP-70 antikorları yakın zaman önce
multipl skleroz (MS) olgularında gösterilmiş ve hastalığın prognozu ile
ilişkili oldukları bulunmuştur. Bu antikorların sinir sistemi üzerine olan
olası etkilerinin aydınlatılması amaçlanmıştır.

Gereç ve Yöntem: SWAP-70 antikoru pozitif
ve negatif olan dörder MS olgusunun ve 5 sağlıklı olgunun serum havuzlarından
IgG saflaştırıldı ve farelerin yan ventriküllerine uygulandı (her grup için
n=10).

Bulgular: Tedavi grupları arasında motor
aktivite testi ile değerlendirilen kas gücü açısından fark saptanmadı. Benzer
şekilde farklı IgG grupları arasında beyin immünohistokimya özellikleri
açısından fark bulunamadı.

Sonuç: Bu pasif transferle oluşturulan MS
hayvan modelinde MS IgG’sinin belirgin bir nörotoksik veya nörokoruyucu etkisi
gösterilemedi.

Keywords

IgG, santral sinir sistemi, Multipl skleroz, lokomotor aktivite, histoloji

Impact of Intraventrícular Administration of SWAP-70 Antibody Positive Multiple Sclerosis Serum Antibodies on Motor Activity and Brain Histology

Abstract

DOI: 10.26650/experimed.2018.377256

Objectives: Antibodies directed against
SWAP-70 have been recently identified in multiple sclerosis (MS) patients and
found to be associated with disease prognosis. Our aim was to find out the
potential actions of these antibodies on the nervous system.

Material and Method: Purified IgG was
obtained from pooled sera from 4 each MS patients with and without SWAP-70
antibodies and 5 healthy controls and administered into lateral ventricles of
mice (n=10 for each group).

Results: There were no significant differences
among treatment groups by means of muscle strength, which was evaluated by
motor activity test. Similarly, no significant differences could be found in
brain immunohistochemistry examinations among different IgG groups.

Conclusion: Our results argue against a
profound neurotoxic or neuroprotective action of MS IgG on neurons in this
passive transfer animal model of MS.

Keywords

Multiple sclerosis, IgG, locomotor activity, central nervous system, histology

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