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Histological and Electron Microscopical Observations on the Testis and Male Accessory Glands of Poecilimon ataturki Ünal, 1999 (Orthoptera, Tettigoniidae)

Year 2021, , 75 - 81, 17.12.2021
https://doi.org/10.26650/EurJBiol.2021.1001795

Abstract

Objective: The literature has many studies in the world about the morphology and histology of insect tissues, especially structures related to reproductive systems. However, there are no studies about the biology of Poecilimon ataturki Ünal, 1999 (Orthoptera, Tettigoniidae). For this reason, we aimed that the morphology and the structure of the testes and the accessory glands in P. ataturki are revealed.

Materials and Methods: In the present study, the histology and morphology of organs of the reproductive system in male P. ataturki, an endemic species in Turkey was examined by light microscope, scanning electron microscope, transmission electron microscope, and stereomicroscope.

Results: The reproductive system of male P. ataturki is composed of two testes, two vas deferens and short and long accessory glands. Each testis includes numerous follicles where the sperm generation occurs in. There are cysts in the follicles, one of which is in a particular sperm development. 3 different development stages are observed in follicles as growth zone, maturation zone and transformation zone. During these developmental stages, first spermatocytes reproduce by mitosis, and then they turn into spermatids by meiosis. Spermatozoa are also formed by the transformation of spermatids. Thus, the stages of spermatogenesis and spermiogenesis are completed. The accessory glands, whose main task is to produce secretion in order to facilitate the feeding of sperm and their transfer to the female, consist of many tubular structures, long and short. It is seen that it consists of single-layered epithelial tissue in cross sections of the accessory gland tubules.

Conclusion: As a consequence of this work, it has been evinced that male reproductive system elements belonging to P. ataturki show high similarity with the male reproductive systems of other species in the Tettigoniidae family.

Thanks

The authors owe Prof. Dr. Mustafa ÜNAL from Abant İzzet Baysal University a great debt of gratitude for diagnosis of the species studied and Gazi University Academic Writing and Research Center for their help and support in the proofreading of the current study.

References

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  • 27. Mari IP, Gigliolli AAS, Nanya S, Portela-Castro ALB. Histological and electron microscopy observations on the testis and spermatogen-esis of the butterfly Dione juno (Cramer, 1779) and Agraulis vanilla (Linnaeus, 1758) (Lepidoptera: Nymphalidae). Micron 2018; 109: 11-21. google scholar
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  • 29. Zhang BB, Lyu QH, Hua BZ. Male reproductive system and sperm ultrastructure of Furcatopanorpa longihypovalva (Hua and Cai, 2009) (Mecoptera, Panorpidae) and its phylogenetic implication. Zool Anz 2016; 246: 41-6. google scholar
  • 30. Pitnick S, Markow TA. Large-male advantages associated with costs of sperm production in Drosophila hydei, a species with giant sperm. Proc Natl Acad Sci 1994; 91(20): 9277-81. google scholar
  • 31. Snook RR. Sperm in competiton: not playing by the numbers. Trends Ecol Evol 2005; 20: 46-53. google scholar
  • 32. Werner M, Simmons LW. Insect sperm motility. Biol Rev 2008; 83: 191-2. google scholar
  • 33. Dallai R, Gottardo M, Beutel RG. Structure and evolution of insect sperm: New interpretations in the age of phylogenomics. Annu Rev Entomol 2016; 61: 1-23. google scholar
  • 34. Happ GM. Maturation of the male reproductive systems and its en-docrine regulation. Annu Rev Entomol 1992; 37: 303-20. google scholar
  • 35. Odhiambo TR. The architecture of the accessory reproductive glands of the male desert locust: III components of the muscular wall. Tissue Cell 1970; 2(2): 233-48. google scholar
  • 36. Gallois D, Cassier P. Cytodifferentiation and maturation in the male accessory glands of Locusta migratoria migratoriodes (R. and F.) (Orthoptera: Acrididae). Int J Insect Morphol Embryol 1991; 20(3): 141-55. google scholar
  • 37. Viscuso R, Narcisi L, Sottile L, Brundo MV. Role of male accessory glands in spermatodesm reorganization in Orthoptera Tettigoni-oidea. Tisssue Cell 2001; 33(1): 33-9. google scholar
Year 2021, , 75 - 81, 17.12.2021
https://doi.org/10.26650/EurJBiol.2021.1001795

Abstract

References

  • 1. Gillott C. Male accessory gland secretions: Modulators of female reproductive physiology and behavior. Annu Rev Entomol 2003; 48: 163-84. google scholar
  • 2. Silva DSM, Cossolin JFS, Pereira MR, Lino-Neto J, Sperber CF, Serrao JE. Male reproductive tract and spermatozoa ultrastructure in the grasshopper Orphulella punctata (De Geer, 1773) (Insecta, Orthop-tera, Caelifera). Microsc Res Tech 2018; 81(2): 250-5. google scholar
  • 3. Stephens K, Cardullo RA, Thaler CD. Culex pipiens sperm motility is initiated by a trypsin-like protease from male accessory glands. Mol Reprod Dev 2018; 85(5): 440-8. google scholar
  • 4. Polat I, Amutkan Mutlu D, Ünal M, Suludere Z. Histology and ultra-structure of the testis and vas deferens in Pseudochorthippus par-allelus parallelus (Orthoptera, Acrididae). Microsc Res Tech 2019; 82(9): 1461-70. google scholar
  • 5. Polat I, Amutkan Mutlu D, Suludere Z. Accessory glands of male reproductive system in Pseudochorthippus parallelus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae): A light and electron microscopic study. Microsc Res Tech 2020; 83(3): 232-8. google scholar
  • 6. White MJD. Patterns of spermatogenesis in grasshoppers. Aust J Zool 1954; 3: 222-6. google scholar
  • 7. Chapman RF. The Insect Structure and Function. 5th ed. UK: Cam-bridge University Press; 2013. google scholar
  • 8. Klowden MJ. Physiological Systems in Insects. 3rd ed. London, UK: Akademic Press; 2013. google scholar
  • 9. Sharma V, Pandey AK, Kumar A, Misra S, Gupta HP, Gupta S, et al. Functional male accessory glands and fertility in Drosophila re-quire novel ecdysone receptor. PLoS Genet 2017; 13(5): 1-26. google scholar
  • 10. Gotoh A, Shigenobu S, Yamaguchi K, Kobayashi S, Ito F, Tsuji K. Transcriptome characterization of male accessory glands in ants to identify molecules involved in their reproductive success. Insect Mol Biol 2018; 27(2): 212-20. google scholar
  • 11. Ünal M. Notes on Orthoptera of Western Turkey, with description of a new genus and four new species. J Orthoptera Res 1999; 8: 243-55. google scholar
  • 12. Ünal M. Notes on Orthoptera of Western Turkey, with description of a new genus and four new species. J Orthoptera Res 2000; 9: 89-102. google scholar
  • 13. Ünal M. Poecilimon Fischer (Orthoptera: Tettigoniidae: Phanerop-terinae) species of the Batı Karadeniz region of Turkey, NW Anato-lia. Trans Am Entomol Soc 2003; 129(3-4): 361-87. google scholar
  • 14. Ünal M. Phaneropterinae (Orthoptera: Tettigoniidae) from Turkey and the Middle East. Trans Am Entomol Soc 2005; 131(3-4): 25-448. google scholar
  • 15. Ünal M. Phaneropterinae (Orthoptera: Tettigoniidae) from Turkey and the Middle East II. Trans. Am Entomol Soc 2010; 136(1-2): 12583. google scholar
  • 16. Liu X, Zhang J, Ma E, Guo Y. Studies on the phylogenetic relation-ship of Acridoidea based on the male follicle morphology (Ortho-ptera: Acridoidea). Orient Insects 2005; 39: 21-32. google scholar
  • 17. Marchini D, Brundo MV, Sottile L, Viscuso R. Structure of male ac-cessory glands of Bolivarus siculus (Fischer) (Orthoptera, Tettigoni-idae) and protein analysis of their secretions. J Morphol 2009; 270: 880-91. google scholar
  • 18. Jones N, Taub-Montemayor T, Rankin MA. Fluorescein-dextran se-questration in the reproductive tract of the migratory grasshopper Melanoplus sanguinipes (Orthoptera, Acridiidae). Micron 2013; 46: 80-4. google scholar
  • 19. Viscuso R, Brundo MV, Marletta A, Vitale DGM. Fine structure of the male genital tracts of some Acrididae and Tettigoniidae (Insect: Or-thoptera). Acta Zool 2015; 96(4): 418-27. google scholar
  • 20. Vitale DGM, Brundo MV, Viscuso R. Morphological and ultrastruc-tural organization of the male genital apparatus of some Aphidi-dae (Insect, Homoptera). Tissue Cell 2011; 43: 271-82. google scholar
  • 21. Nandchahal N. Reproductive organs of Gryllodes sigillatus (Walker) (Orthoptera: Gryllidae). J Nat Hist 1972; 6: 125-31. google scholar
  • 22. Vitale DGM, Viscuso R, D’Urso V, Gibilras S, Sardella A, Marletta A, et al. Morphostructural analysis of the male reproductive system and DNA barcoding in Balclutha brevis Lindberg 1954 (Homoptera, Cicadellidae). Micron 2015; 79: 36-45. google scholar
  • 23. O’woma OO, Chigozirim UP, Emmanuel O, Chukwuebuka EM. Re-productive and survival strategies utilized by insect. A review. Am J Zool Res 2016; 4(1): 1-6. google scholar
  • 24. Kotze RCM, Muller N, Du Plessis L, Van der Horst G. The importance of the insect sperm: Sperm ultrastructure of Hermetia illucens (black soldier fly). Tissue Cell 2019; 59: 44-50. google scholar
  • 25. Sturm R. Stereological analysis of the sperm number in the testicu-lar follicles of the Australian field cricket (Insecta: Orthoptera). Linz Biol Beit 2018; 50(1): 865-72. google scholar
  • 26. Polat I. Poecilimon cervus Karabağ, 1950’un Sindirim, Boşaltım, Dişi ve Erkek Üreme Sisteminin Ültrastrüktürel Özellikleri. G.Ü., Fen Bilimleri Enstitüsü, Doktora Tezi. 2016. google scholar
  • 27. Mari IP, Gigliolli AAS, Nanya S, Portela-Castro ALB. Histological and electron microscopy observations on the testis and spermatogen-esis of the butterfly Dione juno (Cramer, 1779) and Agraulis vanilla (Linnaeus, 1758) (Lepidoptera: Nymphalidae). Micron 2018; 109: 11-21. google scholar
  • 28. Novais AM, Dias G, Lino-Neto J. Testicular, spermatogenesis and sperm morphology in Martarega bentoi Heteroptera: Notonecti-dae). Arthropod Struct Dev 2017; 46: 635-43. google scholar
  • 29. Zhang BB, Lyu QH, Hua BZ. Male reproductive system and sperm ultrastructure of Furcatopanorpa longihypovalva (Hua and Cai, 2009) (Mecoptera, Panorpidae) and its phylogenetic implication. Zool Anz 2016; 246: 41-6. google scholar
  • 30. Pitnick S, Markow TA. Large-male advantages associated with costs of sperm production in Drosophila hydei, a species with giant sperm. Proc Natl Acad Sci 1994; 91(20): 9277-81. google scholar
  • 31. Snook RR. Sperm in competiton: not playing by the numbers. Trends Ecol Evol 2005; 20: 46-53. google scholar
  • 32. Werner M, Simmons LW. Insect sperm motility. Biol Rev 2008; 83: 191-2. google scholar
  • 33. Dallai R, Gottardo M, Beutel RG. Structure and evolution of insect sperm: New interpretations in the age of phylogenomics. Annu Rev Entomol 2016; 61: 1-23. google scholar
  • 34. Happ GM. Maturation of the male reproductive systems and its en-docrine regulation. Annu Rev Entomol 1992; 37: 303-20. google scholar
  • 35. Odhiambo TR. The architecture of the accessory reproductive glands of the male desert locust: III components of the muscular wall. Tissue Cell 1970; 2(2): 233-48. google scholar
  • 36. Gallois D, Cassier P. Cytodifferentiation and maturation in the male accessory glands of Locusta migratoria migratoriodes (R. and F.) (Orthoptera: Acrididae). Int J Insect Morphol Embryol 1991; 20(3): 141-55. google scholar
  • 37. Viscuso R, Narcisi L, Sottile L, Brundo MV. Role of male accessory glands in spermatodesm reorganization in Orthoptera Tettigoni-oidea. Tisssue Cell 2001; 33(1): 33-9. google scholar
There are 37 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Damla Amutkan Mutlu 0000-0002-4780-8520

İrmak Polat 0000-0001-7230-4589

Zekiye Suludere 0000-0002-1207-5814

Publication Date December 17, 2021
Submission Date September 29, 2021
Published in Issue Year 2021

Cite

AMA Amutkan Mutlu D, Polat İ, Suludere Z. Histological and Electron Microscopical Observations on the Testis and Male Accessory Glands of Poecilimon ataturki Ünal, 1999 (Orthoptera, Tettigoniidae). Eur J Biol. December 2021;80(2):75-81. doi:10.26650/EurJBiol.2021.1001795