Research Article
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Year 2022, , 251 - 256, 29.12.2022
https://doi.org/10.26650/EurJBiol.2022.1167842

Abstract

Project Number

Project number of 1919B012004906

References

  • 1. Chanchal DK, Alok S, Rashi S, Bijauliya RK, Yadav RD, Sabharwal M. Various medicinal plants used in the treatment of anticancer activity. Int J Pharm Sci Res 2018; 9(4): 1424-9. google scholar
  • 2. Ohiagu FO, Chikezie PC, Chikezie CM, Enyoh CE. Anticancer activity of Nigerian medicinal plants: a review. Futur J Pharm Sci 2021; 7: 70. google scholar
  • 3. Khalil MIM, Ibrahim MM, El-Gaaly GA, Sultan AS. Trigonella foenum(Fenugreek) Induced Apoptosis in Hepatocellular Carcinoma Cell Line, HepG2, Mediated by Upregulation of p53 and Proliferating Cell Nuclear Antigen. BioMed Res Int 2015; 2015: 914645. google scholar
  • 4. Al-Oqail MM, Farshori NN, Al-Sheddi ES, Musarrat J, Al-Khedhairy AA, Siddiqui MA. In vitro cytotoxic activity of seed oil of fenugreek against various cancer cell lines. Asian Pac J Cancer Prev 2013; 14(3): 1829-32. google scholar
  • 5. Marshall GM, Carter DR, Cheung BB, Liu T, Mateos MK, Meyerowitz JG et al. The prenatal origins of cancer. Nat Rev Cancer 2014; 14(4): 277-89. google scholar
  • 6. Li J, Pang J, Liu Y, Zhang J, Zhang C, Shen G et al. Suppression of RRM2 inhibits cell proliferation, causes cell cycle arrest and promotes the apoptosis of human neuroblastoma cells and in human neuroblastoma RRM2 is suppressed following chemotherapy. Oncol rep 2018; 40(1): 355-60. google scholar
  • 7. Cortelo PC, Demarque DP, Dusi RG, Albernaz LC, Braz-Filho R, Goncharova EI et al. A Molecular Networking Strategy: High-Throughput Screening and Chemical Analysis of Brazilian Cerrado Plant Extracts against Cancer Cells. Cells 2021; 10(3): 691. google scholar
  • 8. Aydemir EA, Simsek E, Imir N, Göktürk RS, Yesilada E, Fiskin K. Cytotoxic and apoptotic effects of Ebenus boissieri Barbey on human lung cancer cell line A549. Pharmacogn Mag 2015; 11(42): 37-45. google scholar
  • 9. Khoja KK, Howes MJR, Hider R, Sharp PA, Farrell IW, Latunde-Dada GO. Cytotoxicity of Fenugreek Sprout and Seed Extracts and Their Bioactive Constituents on MCF-7 Breast Cancer Cells. Nutrients 2022; 14(4): 784. google scholar
  • 10. Wani SA, Kumar P. Fenugreek: A review on its nutraceutical properties and utilization in various food products. J Saudi Soc Agric Sci 2018; 17(2), 97-106. google scholar
  • 11. Rahmati-Yamchi M, Ghareghomi S, Haddadchi G, Milani M, Aghaz-ade M, Daroushnejad H. Fenugreek extract diosgenin and pure diosgenin inhibit the hTERT gene expression in A549 lung cancer cell line. Mol Biol 2014; 41: 6247-52. google scholar
  • 12. Zameer S, Najmi AK, Vohora D, Akhtar M. A review on therapeutic potentials of Trigonella foenum graecum (fenugreek) and its chemical constituents in neurological disorders: Complementary roles to its hypolipidemic, hypoglycemic, and antioxidant potential. Nutr Neurosci 2018; 21(8): 539-45. google scholar
  • 13. Sebastian KS, Thampan RV. Differential effects of soybean and fenugreek extracts on the growth of MCF-7 cells. Chem Biol Interact 2007; 170(2): 135-43. google scholar
  • 14. Khoja KK, Howes MJR, Hider R, Sharp PA, Farrell IW, Latunde-Dada GO. Cytotoxicity of Fenugreek Sprout and Seed Extracts and Their Bioactive Constituents on MCF-7 Breast Cancer Cells. Nutrients 2022; 14(4): 784. google scholar
  • 15. Khoja KK, Shaf G, Hasan TN, Syed NA, Al-Khalifa AS, Al-Assaf AH, Alshatwi AA. Fenugreek, a naturally occurring edible spice, kills MCF-7 human breast cancer cells via an apoptotic pathway. Asian Pac J Cancer Prev 2011; 12(12): 3299-304. google scholar
  • 16. Kaviarasan S, Ramamurty N, Gunasekaran P, Varalakshmi E, Anuradha CV. Fenugreek (Trigonella foenum graecum) seed extract prevents ethanol-induced toxicity and apoptosis in chang liver cells. Alcohol & Alcoholism 2006; 41(3): 267-73. google scholar
  • 17. Syed QA, Rashid Z, Ahmad MH, Shukat R, Ishaq A, Muhammad N, et al. Nutritional and therapeutic properties of fenugreek (Trigonella foenum-graecum): a review. Int J Food Prop 2020; 23: 1777-91. google scholar
  • 18. Verma KS, Singh KS, Mathur A. In vitro cytotoxicity of Calotropis procera and Trigonella foenumgraecum against human cancer cell lines. J Chem Pharm Res 2010; 2(4): 861-5. google scholar
  • 19. Nelson VK, Sahoo NK, Sahu M, Sudhan HH, Pullaiah CP, Muralikrish-na KS. In vitro anticancer activity of Eclipta alba whole plant extract on colon cancer cell HCT-116. BMC Complement Med Ther 2020; 20(1): 355. google scholar
  • 20. Alrumaihi FA, Khan MA, Allemailem KS, Alsahli MA, Almatroudi A, Younus H, et al. Methanolic fenugreek seed extract induces p53-dependent mitotic catastrophe in breast cancer cells, leading to apoptosis. J Inflamm Res 2021; 16(14): 1511-35. google scholar
  • 21. Sitarek P, Kowalczyk T, Santangelo S, Bialas JA, Toma M, Wieczfins-ka J. The Extract of Leonurus sibiricus transgenic roots with AtPAP1 transcriptional factor induces apoptosis via DNA damage and down regulation of selected epigenetic factors in human cancer cells. Neurochem Res 2018; 43: 1363-70. google scholar
  • 22. Alshatwi AA, Shafi G, Hasan TN, Syed NA, Khoja KK. Fenugreek induced apoptosis in breast cancer MCF-7 cells mediated independently by fas receptor change. Asian Pac J Cancer Prev 2013; 14(10): 5783-8. google scholar

Anti-Cancer Effects of Trigonella foenum in Neuroblastoma Cell Line

Year 2022, , 251 - 256, 29.12.2022
https://doi.org/10.26650/EurJBiol.2022.1167842

Abstract

Objective: Current approaches focus on the use of natural compounds in plants in the treatment of cancer. Trigonella foenum (Fenugreek) is mainly used in medicine, food, and cosmetics due to its bioactive and aromatic compounds. Many compounds with medicinal effects are found in Fenugreek seeds. These compounds have been demonstrated to be effective on numerous cancer cells. Neuroblastoma is one of the most common extracranial solid malignancies in children. In our study, the effects of Fenugreek on cell cytotoxicity, cell damage, migration, and sphere formation in neuroblastoma cells were investigated. Materials and Methods: The powder extract was dissolved in DMEM and filtered to prepare a stock solution of Fenugreek. We used MTT assay for the detection of cytotoxic effects of Fenugreek on SH-SY5Y cells, crystal violet staining for the analysis of morphological change in cells, wound healing test for the cell migration analysis, and agarose gel electrophoresis for detecting DNA damage. We created a spheroid to test the effect we observed in 2D culture, in 3D culture as well. Results: The findings of our study showed that Fenugreek treatments caused significant reductions in cell proliferation and migration capacities in 2D neuroblastoma cells at IC50 (1500 μg/ml) doses, respectively. In addition Fenugreek changed cell morphology and increased DNA fragmentation. Furthermore, Fenugreek caused disruption of 3D spheroid formation of SHSY-5Y cells in a dose- and time-dependent manner. Conclusion: The determination of the anti-cancer effect of Fenugreek on neuroblastoma cancer may be a useful and feasible intervention in neuroblastoma patients in the light of further studies and with the help of new nano drug delivery systems.

Supporting Institution

The Scientific and Technological Research Institution

Project Number

Project number of 1919B012004906

Thanks

Our study was approved and supported by The Scientific and Technological Research Institution of Turkey Projects 2209-A by the Project number of 1919B012004906

References

  • 1. Chanchal DK, Alok S, Rashi S, Bijauliya RK, Yadav RD, Sabharwal M. Various medicinal plants used in the treatment of anticancer activity. Int J Pharm Sci Res 2018; 9(4): 1424-9. google scholar
  • 2. Ohiagu FO, Chikezie PC, Chikezie CM, Enyoh CE. Anticancer activity of Nigerian medicinal plants: a review. Futur J Pharm Sci 2021; 7: 70. google scholar
  • 3. Khalil MIM, Ibrahim MM, El-Gaaly GA, Sultan AS. Trigonella foenum(Fenugreek) Induced Apoptosis in Hepatocellular Carcinoma Cell Line, HepG2, Mediated by Upregulation of p53 and Proliferating Cell Nuclear Antigen. BioMed Res Int 2015; 2015: 914645. google scholar
  • 4. Al-Oqail MM, Farshori NN, Al-Sheddi ES, Musarrat J, Al-Khedhairy AA, Siddiqui MA. In vitro cytotoxic activity of seed oil of fenugreek against various cancer cell lines. Asian Pac J Cancer Prev 2013; 14(3): 1829-32. google scholar
  • 5. Marshall GM, Carter DR, Cheung BB, Liu T, Mateos MK, Meyerowitz JG et al. The prenatal origins of cancer. Nat Rev Cancer 2014; 14(4): 277-89. google scholar
  • 6. Li J, Pang J, Liu Y, Zhang J, Zhang C, Shen G et al. Suppression of RRM2 inhibits cell proliferation, causes cell cycle arrest and promotes the apoptosis of human neuroblastoma cells and in human neuroblastoma RRM2 is suppressed following chemotherapy. Oncol rep 2018; 40(1): 355-60. google scholar
  • 7. Cortelo PC, Demarque DP, Dusi RG, Albernaz LC, Braz-Filho R, Goncharova EI et al. A Molecular Networking Strategy: High-Throughput Screening and Chemical Analysis of Brazilian Cerrado Plant Extracts against Cancer Cells. Cells 2021; 10(3): 691. google scholar
  • 8. Aydemir EA, Simsek E, Imir N, Göktürk RS, Yesilada E, Fiskin K. Cytotoxic and apoptotic effects of Ebenus boissieri Barbey on human lung cancer cell line A549. Pharmacogn Mag 2015; 11(42): 37-45. google scholar
  • 9. Khoja KK, Howes MJR, Hider R, Sharp PA, Farrell IW, Latunde-Dada GO. Cytotoxicity of Fenugreek Sprout and Seed Extracts and Their Bioactive Constituents on MCF-7 Breast Cancer Cells. Nutrients 2022; 14(4): 784. google scholar
  • 10. Wani SA, Kumar P. Fenugreek: A review on its nutraceutical properties and utilization in various food products. J Saudi Soc Agric Sci 2018; 17(2), 97-106. google scholar
  • 11. Rahmati-Yamchi M, Ghareghomi S, Haddadchi G, Milani M, Aghaz-ade M, Daroushnejad H. Fenugreek extract diosgenin and pure diosgenin inhibit the hTERT gene expression in A549 lung cancer cell line. Mol Biol 2014; 41: 6247-52. google scholar
  • 12. Zameer S, Najmi AK, Vohora D, Akhtar M. A review on therapeutic potentials of Trigonella foenum graecum (fenugreek) and its chemical constituents in neurological disorders: Complementary roles to its hypolipidemic, hypoglycemic, and antioxidant potential. Nutr Neurosci 2018; 21(8): 539-45. google scholar
  • 13. Sebastian KS, Thampan RV. Differential effects of soybean and fenugreek extracts on the growth of MCF-7 cells. Chem Biol Interact 2007; 170(2): 135-43. google scholar
  • 14. Khoja KK, Howes MJR, Hider R, Sharp PA, Farrell IW, Latunde-Dada GO. Cytotoxicity of Fenugreek Sprout and Seed Extracts and Their Bioactive Constituents on MCF-7 Breast Cancer Cells. Nutrients 2022; 14(4): 784. google scholar
  • 15. Khoja KK, Shaf G, Hasan TN, Syed NA, Al-Khalifa AS, Al-Assaf AH, Alshatwi AA. Fenugreek, a naturally occurring edible spice, kills MCF-7 human breast cancer cells via an apoptotic pathway. Asian Pac J Cancer Prev 2011; 12(12): 3299-304. google scholar
  • 16. Kaviarasan S, Ramamurty N, Gunasekaran P, Varalakshmi E, Anuradha CV. Fenugreek (Trigonella foenum graecum) seed extract prevents ethanol-induced toxicity and apoptosis in chang liver cells. Alcohol & Alcoholism 2006; 41(3): 267-73. google scholar
  • 17. Syed QA, Rashid Z, Ahmad MH, Shukat R, Ishaq A, Muhammad N, et al. Nutritional and therapeutic properties of fenugreek (Trigonella foenum-graecum): a review. Int J Food Prop 2020; 23: 1777-91. google scholar
  • 18. Verma KS, Singh KS, Mathur A. In vitro cytotoxicity of Calotropis procera and Trigonella foenumgraecum against human cancer cell lines. J Chem Pharm Res 2010; 2(4): 861-5. google scholar
  • 19. Nelson VK, Sahoo NK, Sahu M, Sudhan HH, Pullaiah CP, Muralikrish-na KS. In vitro anticancer activity of Eclipta alba whole plant extract on colon cancer cell HCT-116. BMC Complement Med Ther 2020; 20(1): 355. google scholar
  • 20. Alrumaihi FA, Khan MA, Allemailem KS, Alsahli MA, Almatroudi A, Younus H, et al. Methanolic fenugreek seed extract induces p53-dependent mitotic catastrophe in breast cancer cells, leading to apoptosis. J Inflamm Res 2021; 16(14): 1511-35. google scholar
  • 21. Sitarek P, Kowalczyk T, Santangelo S, Bialas JA, Toma M, Wieczfins-ka J. The Extract of Leonurus sibiricus transgenic roots with AtPAP1 transcriptional factor induces apoptosis via DNA damage and down regulation of selected epigenetic factors in human cancer cells. Neurochem Res 2018; 43: 1363-70. google scholar
  • 22. Alshatwi AA, Shafi G, Hasan TN, Syed NA, Khoja KK. Fenugreek induced apoptosis in breast cancer MCF-7 cells mediated independently by fas receptor change. Asian Pac J Cancer Prev 2013; 14(10): 5783-8. google scholar
There are 22 citations in total.

Details

Primary Language English
Journal Section Themed Articles - Research Articles
Authors

İrem Ürkmez This is me 0000-0002-5466-3323

Hatice İlayhan Karahan Çöven This is me 0000-0002-3371-7345

Aslı Eldem This is me 0000-0003-3510-6748

Melek Pehlivan 0000-0001-8755-4812

Project Number Project number of 1919B012004906
Publication Date December 29, 2022
Submission Date August 30, 2022
Published in Issue Year 2022

Cite

AMA Ürkmez İ, Karahan Çöven Hİ, Eldem A, Pehlivan M. Anti-Cancer Effects of Trigonella foenum in Neuroblastoma Cell Line. Eur J Biol. December 2022;81(2):251-256. doi:10.26650/EurJBiol.2022.1167842