Di-(2-ethylhexyl)-phthalate
(DEHP) is used in polyethylene terephthalate plastics and bisphenol-A (BPA) is
used to make polycarbonate plastics. The health-concerning effects of DEHP and
BPA indicate their potential toxic effects. The Wnt/β-catenin signaling pathway is necessary for embryonic
development. The c-MYC proto-oncogene (MYC) has been identified as a Wnt/β-catenin target gene, and MYC is a transcription
factor that regulates the expression of many gene products related to cell
proliferation, growth, differentiation, and apoptosis. Activation and
overexpression of the MYC gene has been reported in many human cancers and
during tumorigenesis. The aim of this study was to investigate the relationship
between oxidant-antioxidant balance and c-myc in BPA- and DEHP-exposed
zebrafish embryos. The real-time-polymerase chain reaction was used to
determine c-myc expression. Lipid peroxidation (LPO), nitric oxide (NO) levels,
and superoxide dismutase (SOD) and glutathione-S-transferase (GST) activities
were determined in homogenates prepared from pooled embryo samples. Increased
expression of c-myc and decreased GST activity were observed in the BPA and
DEHP groups. NO levels increased and SOD activity decreased in the BPA group,
whereas LPO increased in the DEHP group. Disruption of oxidant-antioxidant
balance in DEHP- and BPA-exposed zebrafish embryos was associated with
increased c-myc expression and may be an important mechanism for the toxic
effects of these chemicals.
Journal Section | Research Articles |
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Authors | |
Publication Date | December 27, 2017 |
Submission Date | October 16, 2017 |
Published in Issue | Year 2017 Volume: 76 Issue: 1 |