Research Article
BibTex RIS Cite

Karyotypes of Southeastern Turkish Scorpions Hottentotta saulcyi and Buthacus macrocentrus (Scorpiones: Buthidae)

Year 2019, Volume: 78 Issue: 2, 111 - 116, 06.12.2019

Abstract

Objective: The species Hottentotta saulcyi is widely distributed from Mardin to Hakkari while the distribution of Buthacus macrocentrus is limited to the south-east of Turkey (only Şanlıurfa). The aim of this study is to analyze the cytogenetic structure of Hottentotta saulcyi and Buthacus macrocentrus. Materials and Methods: The specimens were collected during the night from Şırnak and Şanlıurfa using a UV lamp. The male Hottentotta saulcyi were collected from Şırnak and male and female Buthacus macrocentrus from Şanlıurfa. Chromosome preparations were made using cells from the male testes and the female ovariuteri of the species studied. Chromosome preparations were made using the classical spreading method. Results: The diploid chromosome number for Hottentotta saulcyi was 2n=14, and 2n=28 for Buthacus macrocentrus. Conclusion: The karyotypes of Hottentotta saulcyi and Buthacus macrocentrus have been presented for the first time. Both analyzed species have holocentric chromosomes that gradually decrease in size. Quadrivalent and hexavalent were observed during the first meiotic division in males of Buthacus macrocentrus. 

Supporting Institution

The project was financially supported by the Sinop University BAP Unit.

Project Number

FEF 1901-13-03

References

  • 1. Rein JO. The Scorpion Files. Trondheim: Norwegian University of Science and Technology. [Accessed 2019.01.23]. Available from https://www.ntnu.no/ub/scorpion-files 2019.
  • 2. Dunlop JA, Penney D. Fossil Arachnids. Monograph Series Volume 2. Siri Scientific Press, Manchester 2012; 192 pp.
  • 3. Schneider MC, Mattos VF, Cella DM. The scorpion cytogenetic database. Available in www.arthropodacytogenetics.bio.br/ scorpiondatabase 2019.
  • 4. Mattos VF, Cella DM, Carvalho LS, Candido DM, Schneider MC. High chromosome variability and the presence of multivalent associations in buthid scorpions. Chrom Res 2013; 21:121–36.
  • 5. Šťáhlavský F, Koç H, Yağmur EA. The first record of karyotypes in Leiurus abdullahbayrami and Compsobuthus matthiesseni (Scorpiones: Buthidae) from Turkey. North-West J Zool 2014; 10 (2): 355-8.
  • 6. Sadílek D, Nguyen P, Koç H, Kovarik F, Yağmur EA, Šťáhlavský F. Molecular cytogenetics of Androctonus scorpions: an oasis of calm in the turbulent karyotype evolution of the diverse family Buthidae. Biol J Linn Soc 2015; 115: 69-76.
  • 7. Karataş Ay, Uçak M, Karataş Ah, Eroğlu O. Records of chromosomal data of some scorpions (Arachnida: Scorpiones) from Turkey. Hacettepe J Biol&Chem 2019; 47(1): 1-6.
  • 8. Kovařík F. A revision of the genus Hottentotta Birula, 1908, with descriptions of four new species (Scorpiones, Buthidae). Euscorpius 2007; 58: 1–109.
  • 9. Yağmur EA, Koç K, Yalçın M. Distribution of Hottentotta saulcyi (Simon 1880) (Scorpiones: Buthidae) in Turkey. Euscorpius 2008; 76: 1– 8.
  • 10. Crucitti P, Vignoli V. Gli scorpioni (Scorpiones) dell’Anatolia sudorientale (Turchia). Boll Mus Reg Sci Nat 2002; 19 (2): 433-80.
  • 11. Qumsiyeh MB, Salman INA, Salsaa’ M, Amr ZS. Records of scorpions from the Palestinian Territories, with the first chromosomal data (Arachnida: Scorpiones). Zool Middle East 2013; 59: 70–6.
  • 12. Sharma GP, Parshad R, Joneja MG. Chromosome mechanism in the males of three species of scorpions (Scorpiones–Buthidae). Res Bull Panjab Uni 1959; 10: 197-207.
  • 13. Venkatanarasimhiah CB, Rajasekarasetty MR. Contributions to the cytology of Indian scorpions. Experientia 1964; 21: 154.
  • 14. Gupta AKD, Sarker SK. A study of the meiotic chromosomes of the scorpion Buthus tamulus Fabr. Curr Sci 1965; 2: 54-5.
  • 15. Newlands G, Martindale CB. The buthid scorpion fauna of ZimbabweRhodesia with checklists and keys to the genera and species, distributions and medical importance (Arachnida: Scorpiones). South African Ins Med Res 1980; 67: 51-77.
  • 16. Levy G, Amitai P. Scorpiones, In: Fauna Palaestina, Arachnida I., Israel Acad. Sci. Human., Jerusalem 1980; 130 pp.
  • 17. Fet V, Lowe G. Family Buthidae. pp. 54-286. In: Fet V, Sissom WD, Lowe G, Braunwalder ME. Catalog of the Scorpions of the World (1758-1998). The New York Entomological Society, New York 2000; 690 pp.
  • 18. Kovařík F. Taxonomic position of species of the genus Buthacus Birula, 1908 Described by Ehrenberg and Lourenço, and description of a new species (Scorpiones: Buthidae). Euscorpius 2005; 28: 1–13.
  • 19. Lourenço WR. Further considerations on the genus Buthacus Birula, 1908 (Scorpiones, Buthidae), with a description of one new species and two new subspecies. Bol SEA 2006; 38: 59-70.
  • 20. Yağmur EA, Yalçın M, Çalışır G. Distribution of Androctonus crassicauda (Olivier, 1807) and Buthacus macrocentrus (Ehrenberg, 1828) (Scorpiones: Buthidae) in Turkey. Serket 2008; 11(1): 13-8.
  • 21. Kovařík F, Lowe G, Šťáhlavský F. Review of northwestern African Buthacus, with description of Buthacus stockmanni sp. n. from Morocco and western Sahara (Scorpiones, Buthidae). Euscorpius 2016; 236: 1-18.
  • 22. Traut W. Pachytene mapping in the female silkworm, Bombyx mori L. (Lepidoptera). Chromosoma 1976; 58: 275-84.
  • 23. Schneider C.A., Rasband W.S., Eliceiri K.W. NIH Image to ImageJ: 25 years of image analysis. Nat Meth 2012; 9: 671–5.
  • 24. Sakamoto, Y., Zacaro A.A. LEVAN, an ImageJ plugin for morphological cytogenetic analysis of mitotic and meiotic chromosomes. http:// rsbweb.nih.gov/ij/plugins/levan/levan.html 2009.
  • 25. Shanahan C. Cytogenetics of Australian scorpions. I. Interchange polymorphism in the family Buthidae. Genome 1989; 32:882–9.
  • 26. Shanahan C., Hayman, D. Synaptonemal complex formation in male scorpions exhibiting achiasmate meiosis and structural heterozygosity. Genome 1990; 33: 914–26
Year 2019, Volume: 78 Issue: 2, 111 - 116, 06.12.2019

Abstract

Project Number

FEF 1901-13-03

References

  • 1. Rein JO. The Scorpion Files. Trondheim: Norwegian University of Science and Technology. [Accessed 2019.01.23]. Available from https://www.ntnu.no/ub/scorpion-files 2019.
  • 2. Dunlop JA, Penney D. Fossil Arachnids. Monograph Series Volume 2. Siri Scientific Press, Manchester 2012; 192 pp.
  • 3. Schneider MC, Mattos VF, Cella DM. The scorpion cytogenetic database. Available in www.arthropodacytogenetics.bio.br/ scorpiondatabase 2019.
  • 4. Mattos VF, Cella DM, Carvalho LS, Candido DM, Schneider MC. High chromosome variability and the presence of multivalent associations in buthid scorpions. Chrom Res 2013; 21:121–36.
  • 5. Šťáhlavský F, Koç H, Yağmur EA. The first record of karyotypes in Leiurus abdullahbayrami and Compsobuthus matthiesseni (Scorpiones: Buthidae) from Turkey. North-West J Zool 2014; 10 (2): 355-8.
  • 6. Sadílek D, Nguyen P, Koç H, Kovarik F, Yağmur EA, Šťáhlavský F. Molecular cytogenetics of Androctonus scorpions: an oasis of calm in the turbulent karyotype evolution of the diverse family Buthidae. Biol J Linn Soc 2015; 115: 69-76.
  • 7. Karataş Ay, Uçak M, Karataş Ah, Eroğlu O. Records of chromosomal data of some scorpions (Arachnida: Scorpiones) from Turkey. Hacettepe J Biol&Chem 2019; 47(1): 1-6.
  • 8. Kovařík F. A revision of the genus Hottentotta Birula, 1908, with descriptions of four new species (Scorpiones, Buthidae). Euscorpius 2007; 58: 1–109.
  • 9. Yağmur EA, Koç K, Yalçın M. Distribution of Hottentotta saulcyi (Simon 1880) (Scorpiones: Buthidae) in Turkey. Euscorpius 2008; 76: 1– 8.
  • 10. Crucitti P, Vignoli V. Gli scorpioni (Scorpiones) dell’Anatolia sudorientale (Turchia). Boll Mus Reg Sci Nat 2002; 19 (2): 433-80.
  • 11. Qumsiyeh MB, Salman INA, Salsaa’ M, Amr ZS. Records of scorpions from the Palestinian Territories, with the first chromosomal data (Arachnida: Scorpiones). Zool Middle East 2013; 59: 70–6.
  • 12. Sharma GP, Parshad R, Joneja MG. Chromosome mechanism in the males of three species of scorpions (Scorpiones–Buthidae). Res Bull Panjab Uni 1959; 10: 197-207.
  • 13. Venkatanarasimhiah CB, Rajasekarasetty MR. Contributions to the cytology of Indian scorpions. Experientia 1964; 21: 154.
  • 14. Gupta AKD, Sarker SK. A study of the meiotic chromosomes of the scorpion Buthus tamulus Fabr. Curr Sci 1965; 2: 54-5.
  • 15. Newlands G, Martindale CB. The buthid scorpion fauna of ZimbabweRhodesia with checklists and keys to the genera and species, distributions and medical importance (Arachnida: Scorpiones). South African Ins Med Res 1980; 67: 51-77.
  • 16. Levy G, Amitai P. Scorpiones, In: Fauna Palaestina, Arachnida I., Israel Acad. Sci. Human., Jerusalem 1980; 130 pp.
  • 17. Fet V, Lowe G. Family Buthidae. pp. 54-286. In: Fet V, Sissom WD, Lowe G, Braunwalder ME. Catalog of the Scorpions of the World (1758-1998). The New York Entomological Society, New York 2000; 690 pp.
  • 18. Kovařík F. Taxonomic position of species of the genus Buthacus Birula, 1908 Described by Ehrenberg and Lourenço, and description of a new species (Scorpiones: Buthidae). Euscorpius 2005; 28: 1–13.
  • 19. Lourenço WR. Further considerations on the genus Buthacus Birula, 1908 (Scorpiones, Buthidae), with a description of one new species and two new subspecies. Bol SEA 2006; 38: 59-70.
  • 20. Yağmur EA, Yalçın M, Çalışır G. Distribution of Androctonus crassicauda (Olivier, 1807) and Buthacus macrocentrus (Ehrenberg, 1828) (Scorpiones: Buthidae) in Turkey. Serket 2008; 11(1): 13-8.
  • 21. Kovařík F, Lowe G, Šťáhlavský F. Review of northwestern African Buthacus, with description of Buthacus stockmanni sp. n. from Morocco and western Sahara (Scorpiones, Buthidae). Euscorpius 2016; 236: 1-18.
  • 22. Traut W. Pachytene mapping in the female silkworm, Bombyx mori L. (Lepidoptera). Chromosoma 1976; 58: 275-84.
  • 23. Schneider C.A., Rasband W.S., Eliceiri K.W. NIH Image to ImageJ: 25 years of image analysis. Nat Meth 2012; 9: 671–5.
  • 24. Sakamoto, Y., Zacaro A.A. LEVAN, an ImageJ plugin for morphological cytogenetic analysis of mitotic and meiotic chromosomes. http:// rsbweb.nih.gov/ij/plugins/levan/levan.html 2009.
  • 25. Shanahan C. Cytogenetics of Australian scorpions. I. Interchange polymorphism in the family Buthidae. Genome 1989; 32:882–9.
  • 26. Shanahan C., Hayman, D. Synaptonemal complex formation in male scorpions exhibiting achiasmate meiosis and structural heterozygosity. Genome 1990; 33: 914–26
There are 26 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Halil Koc 0000-0003-0429-2824

Ersen Aydin Yagmur This is me 0000-0002-0396-3975

Frantisek šťáhlavský This is me 0000-0002-8520-9166

Project Number FEF 1901-13-03
Publication Date December 6, 2019
Submission Date May 16, 2019
Published in Issue Year 2019 Volume: 78 Issue: 2

Cite

AMA Koc H, Yagmur EA, šťáhlavský F. Karyotypes of Southeastern Turkish Scorpions Hottentotta saulcyi and Buthacus macrocentrus (Scorpiones: Buthidae). Eur J Biol. December 2019;78(2):111-116.