Year 2020, Volume 79 , Issue 2, Pages 151 - 156 2020-12-25

Evaluation of In vitro Wound Healing Activity of Thymoquinone

Murat PEKMEZ [1] , Nihan Sultan MILAT [2]


Objective: Nigella sativa has been extensively investigated as an important potential agent for the healing of wounds and there have been numerous studies regarding its effect. Although thymoquinone (TQ) is a well-known active constituent of Nigella sativa, studies in to the usability of TQ on wound healing are still insufficient. In this study, we aimed to evaluate the in vitro wound healing potential of TQ. Materials and Methods: NIH/3T3 mouse embryonic fibroblast cells were used to evaluate the wound healing effect of TQ. Different concentrations of TQ (0.1, 1 and 10 μM) were applied to the cells and their cytotoxic effect on cells after 24- and 48- hours was measured by MTT assay. Its effect on wound healing after 18- and 24- hours recovery was examined by in vitro scratch assay. Also, the level of β-catenin, an effective protein in the process of healing wounds, was determined by Western blot assay. Results: MTT analysis indicated that 0.1, 1 and 10 μM doses of TQ had increased the cell numbers. In vitro scratch assay data showed that treatment with 1 and 10 μM TQ resulted in a statistically significant wound closure activity (91.35% and 90.84%, respectively) compared to the control. Additionally, we observed a statistically significant increase in the β-catenin protein level which supported our data. Conclusion: Our results demonstrated that TQ increases both the viability of NIH/3T3 cells and its wound closure activity in vitro, and that it has the effect of increasing crucial protein β-catenin. This study suggests that TQ may be a valuable substance for the healing of wounds and that its usability should be investigated.
Thymoquinone, wound healing, NIH/3T3
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Primary Language en
Subjects Science
Journal Section Research Articles
Authors

Orcid: 0000-0002-6150-8372
Author: Murat PEKMEZ (Primary Author)
Institution: ISTANBUL UNIVERSITY
Country: Turkey


Orcid: 0000-0002-0795-8030
Author: Nihan Sultan MILAT
Institution: ISTANBUL UNIVERSITY
Country: Turkey


Supporting Institution There are no funders to report for this submission.
Dates

Application Date : January 2, 2020
Acceptance Date : June 30, 2020
Publication Date : December 25, 2020

Bibtex @research article { iufsjb806654, journal = {European Journal of Biology}, issn = {2602-2575}, eissn = {2618-6144}, address = {}, publisher = {Istanbul University}, year = {2020}, volume = {79}, pages = {151 - 156}, doi = {}, title = {Evaluation of In vitro Wound Healing Activity of Thymoquinone}, key = {cite}, author = {Pekmez, Murat and Mılat, Nihan Sultan} }
APA Pekmez, M , Mılat, N . (2020). Evaluation of In vitro Wound Healing Activity of Thymoquinone . European Journal of Biology , 79 (2) , 151-156 . Retrieved from https://dergipark.org.tr/en/pub/iufsjb/issue/55111/806654
MLA Pekmez, M , Mılat, N . "Evaluation of In vitro Wound Healing Activity of Thymoquinone" . European Journal of Biology 79 (2020 ): 151-156 <https://dergipark.org.tr/en/pub/iufsjb/issue/55111/806654>
Chicago Pekmez, M , Mılat, N . "Evaluation of In vitro Wound Healing Activity of Thymoquinone". European Journal of Biology 79 (2020 ): 151-156
RIS TY - JOUR T1 - Evaluation of In vitro Wound Healing Activity of Thymoquinone AU - Murat Pekmez , Nihan Sultan Mılat Y1 - 2020 PY - 2020 N1 - DO - T2 - European Journal of Biology JF - Journal JO - JOR SP - 151 EP - 156 VL - 79 IS - 2 SN - 2602-2575-2618-6144 M3 - UR - Y2 - 2020 ER -
EndNote %0 European Journal of Biology Evaluation of In vitro Wound Healing Activity of Thymoquinone %A Murat Pekmez , Nihan Sultan Mılat %T Evaluation of In vitro Wound Healing Activity of Thymoquinone %D 2020 %J European Journal of Biology %P 2602-2575-2618-6144 %V 79 %N 2 %R %U
ISNAD Pekmez, Murat , Mılat, Nihan Sultan . "Evaluation of In vitro Wound Healing Activity of Thymoquinone". European Journal of Biology 79 / 2 (December 2020): 151-156 .
AMA Pekmez M , Mılat N . Evaluation of In vitro Wound Healing Activity of Thymoquinone. Eur J Biol. 2020; 79(2): 151-156.
Vancouver Pekmez M , Mılat N . Evaluation of In vitro Wound Healing Activity of Thymoquinone. European Journal of Biology. 2020; 79(2): 151-156.
IEEE M. Pekmez and N. Mılat , "Evaluation of In vitro Wound Healing Activity of Thymoquinone", European Journal of Biology, vol. 79, no. 2, pp. 151-156, Dec. 2020