Year 2020, Volume 79 , Issue 2, Pages 89 - 97 2020-12-25

Proteomic Analysis of the Protective Effect of Sodium Nitroprusside on Leaves of Barley Stressed by Salinity

Mustafa YILDIZ [1] , Melike CELIK [2] , Hakan TERZI [3]

Objective: The salinization of agricultural soils poses a serious challenge across the world. Although recent studies have shown that exogenous sodium nitroprusside (SNP) application can alleviate the harmful effects of salinity, the roles of SNP in the regulation of proteomic changes remain poorly understood. Materials and Methods: To unravel the protective roles of exogenous SNP in alleviating salt-induced damage in barley (Hordeum vulgare L.), proteomic analysis was carried out on the leaves of seedlings exposed to 100 mM NaCl stress following 200 M SNP pre-treatment. Results: Our results indicated that SNP pre-treatment restored the seedling growth reduced by salinity stress. Comparing 2-DE gels from the treatments showed that 24 proteins were differentially accumulated under SNP and/or NaCl stress treatments. Among them, 15 proteins were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Gene ontology analysis demonstrated that several pathways were regulated by SNP and/or NaCl treatments, including photosynthesis, protein metabolism, stress defense, and energy metabolism. Exogenous SNP increased the expression levels of 20 kDa chaperonin, proteasome subunit beta type-2, 2-Cys peroxiredoxin BAS1, ferredoxin-NADP reductase, thiazole biosynthetic enzyme 1-1, S-adenosylmethionine synthetase 3, and elongation factor Tu proteins in the leaves of barley seedlings under NaCl stress. Conclusion: Our results indicate that SNP pre-treatment may induce salinity tolerance growth inhibition through regulation of photosynthesis, activation of stress defence, degradation of damaged proteins, and the promoting of the synthesis of polyamines, proline, and GABA
Barley, Nitric oxide, Salinity stress, Sodium nitroprusside, Proteomics
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Primary Language en
Subjects Science
Journal Section Research Articles

Orcid: 0000-0002-6819-9891
Author: Mustafa YILDIZ (Primary Author)
Country: Turkey

Author: Melike CELIK
Country: Turkey

Author: Hakan TERZI
Country: Turkey

Supporting Institution The support of Afyon Kocatepe University Research Fund (Project No. 18.FEN.BİL.01) to this research is thankfully acknowledged.
Project Number 18.FEN.BİL.01
Thanks The authors gratefully acknowledge the Medicinal Genetics Laboratory of Afyonkarahisar Health Sciences University and the DEKART Proteomics Laboratory of Kocaeli University for their technical help. The authors also wish to thank Afyon Kocatepe University’s Foreign Language Support Unit for language editing.

Application Date : June 18, 2020
Acceptance Date : September 26, 2020
Publication Date : December 25, 2020

Bibtex @research article { iufsjb817025, journal = {European Journal of Biology}, issn = {2602-2575}, eissn = {2618-6144}, address = {}, publisher = {Istanbul University}, year = {2020}, volume = {79}, pages = {89 - 97}, doi = {}, title = {Proteomic Analysis of the Protective Effect of Sodium Nitroprusside on Leaves of Barley Stressed by Salinity}, key = {cite}, author = {Yıldız, Mustafa and Celık, Melike and Terzı, Hakan} }
APA Yıldız, M , Celık, M , Terzı, H . (2020). Proteomic Analysis of the Protective Effect of Sodium Nitroprusside on Leaves of Barley Stressed by Salinity . European Journal of Biology , 79 (2) , 89-97 . Retrieved from
MLA Yıldız, M , Celık, M , Terzı, H . "Proteomic Analysis of the Protective Effect of Sodium Nitroprusside on Leaves of Barley Stressed by Salinity" . European Journal of Biology 79 (2020 ): 89-97 <>
Chicago Yıldız, M , Celık, M , Terzı, H . "Proteomic Analysis of the Protective Effect of Sodium Nitroprusside on Leaves of Barley Stressed by Salinity". European Journal of Biology 79 (2020 ): 89-97
RIS TY - JOUR T1 - Proteomic Analysis of the Protective Effect of Sodium Nitroprusside on Leaves of Barley Stressed by Salinity AU - Mustafa Yıldız , Melike Celık , Hakan Terzı Y1 - 2020 PY - 2020 N1 - DO - T2 - European Journal of Biology JF - Journal JO - JOR SP - 89 EP - 97 VL - 79 IS - 2 SN - 2602-2575-2618-6144 M3 - UR - Y2 - 2020 ER -
EndNote %0 European Journal of Biology Proteomic Analysis of the Protective Effect of Sodium Nitroprusside on Leaves of Barley Stressed by Salinity %A Mustafa Yıldız , Melike Celık , Hakan Terzı %T Proteomic Analysis of the Protective Effect of Sodium Nitroprusside on Leaves of Barley Stressed by Salinity %D 2020 %J European Journal of Biology %P 2602-2575-2618-6144 %V 79 %N 2 %R %U
ISNAD Yıldız, Mustafa , Celık, Melike , Terzı, Hakan . "Proteomic Analysis of the Protective Effect of Sodium Nitroprusside on Leaves of Barley Stressed by Salinity". European Journal of Biology 79 / 2 (December 2020): 89-97 .
AMA Yıldız M , Celık M , Terzı H . Proteomic Analysis of the Protective Effect of Sodium Nitroprusside on Leaves of Barley Stressed by Salinity. Eur J Biol. 2020; 79(2): 89-97.
Vancouver Yıldız M , Celık M , Terzı H . Proteomic Analysis of the Protective Effect of Sodium Nitroprusside on Leaves of Barley Stressed by Salinity. European Journal of Biology. 2020; 79(2): 89-97.
IEEE M. Yıldız , M. Celık and H. Terzı , "Proteomic Analysis of the Protective Effect of Sodium Nitroprusside on Leaves of Barley Stressed by Salinity", European Journal of Biology, vol. 79, no. 2, pp. 89-97, Dec. 2020